Kinetic and spectral resolution of multiple nonphotochemical quenching components in Arabidopsis leaves.

Abstract:

:Using novel specially designed instrumentation, fluorescence emission spectra were recorded from Arabidopsis (Arabidopsis thaliana) leaves during the induction period of dark to high-light adaptation in order to follow the spectral changes associated with the formation of nonphotochemical quenching. In addition to an overall decrease of photosystem II fluorescence (quenching) across the entire spectrum, high light induced two specific relative changes in the spectra: (1) a decrease of the main emission band at 682 nm relative to the far-red (750-760 nm) part of the spectrum (Delta F(682)); and (2) an increase at 720 to 730 nm (Delta F(720)) relative to 750 to 760 nm. The kinetics of the two relative spectral changes and their dependence on various mutants revealed that they do not originate from the same process but rather from at least two independent processes. The Delta F(720) change is specifically associated with the rapidly reversible energy-dependent quenching. Comparison of the wild-type Arabidopsis with mutants unable to produce or overexpressing the PsbS subunit of photosystem II showed that PsbS was a necessary component for Delta F(720). The spectral change Delta F(682) is induced both by energy-dependent quenching and by PsbS-independent mechanism(s). A third novel quenching process, independent from both PsbS and zeaxanthin, is activated by a high turnover rate of photosystem II. Its induction and relaxation occur on a time scale of a few minutes. Analysis of the spectral inhomogeneity of nonphotochemical quenching allows extraction of mechanistically valuable information from the fluorescence induction kinetics when registered in a spectrally resolved fashion.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Lambrev PH,Nilkens M,Miloslavina Y,Jahns P,Holzwarth AR

doi

10.1104/pp.109.148213

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

1611-24

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.109.148213

journal_volume

152

pub_type

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