Abstract:
:The kinetic properties of the adenosine 5[prime]-diphosphate/adenosine 5[prime]-triphosphate (ADP/ATP) translocator from pea (Pisum sativum L.) root plastids were determined by silicone oil filtering centrifugation and compared with those of spinach (Spinacia oleracea L.) chloroplasts and pea leaf mitochondria. In addition, the ADP/ATP transporting activities from the above organelles were reconstituted into liposomes. The Km(ATP) value of the pea root ADP/ATP translocator was 10 [mu]M and that for ADP was 46 [mu]M. Corresponding values of the spinach ADP/ATP translocator were 25 [mu]M and 28 [mu]M, respectively. Comparable results were obtained for the reconstituted ATP transport activities. The transport was highly specific for ATP and ADP. Adenosine 5[prime]-monophosphate (AMP) caused only a slight inhibition and phosphoenolpyruvate and inorganic pyrophosphate caused no inhibition of ATP uptake. With pea root plastids and spinach chloroplasts, Km values >1 mM were obtained for ADP-glucose. Since the concentrations of ATP and ADP-glucose in the cytosolic compartment of spinach leaves have been determined as 2.5 and 0.6 mM, respectively, a transport of ADP-glucose by the ADP/ATP translocator does not appear to have any physiological significance in vivo. Although both the plastidial and the mitochondrial ADP/ATP translocators were inhibited to some extent by carboxyatractyloside, no immunological cross-reactivity was detected between the plastidial and the mitochondrial proteins. It seems probable that these proteins derive from different ancestors.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Schunemann D,Borchert S,Flugge UI,Heldt HWdoi
10.1104/pp.103.1.131subject
Has Abstractpub_date
1993-09-01 00:00:00pages
131-137issue
1eissn
0032-0889issn
1532-2548pii
103/1/131journal_volume
103pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The fluorescent compound trisodium, 3-hydroxy-5,8,10-pyrenetrisulfonate (PTS) was used to quantify the apoplastic flux through red pine (Pinus resinosa Ait.) root systems-that portion of the total water flux reaching the xylem without ever crossing a semipermeable membrane. Flow was induced by pressure through detoppe...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.1.21
更新日期:1985-01-01 00:00:00
abstract::Despite generating an obvious mutant phenotype, whether the Arabidopsis (Arabidopsis thaliana) proton gradient regulation5 (pgr5) mutation influences cyclic electron transport (CET) around PSI is a topic of debate. Results of electrochromic shift analysis show that proton conductivity across the thylakoid membrane (gH...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00850
更新日期:2020-09-01 00:00:00
abstract::The absorption characteristics of Cd(2+) by 10- to 12-day-old soybean plants (Glycine max cv Williams) were investigated with respect to influence of Cd concentration on adsorption to root surfaces, root absorption, transport kinetics and interaction with the nutrient cations Cu(2+), Fe(2+), Mn(2+), and Zn(2+). The fr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.3.844
更新日期:1983-11-01 00:00:00
abstract::Two maize hybrids were grown under growth chamber conditions on solution or vermiculite medium that contained 2.5, 7.5, or 15 millimolar nitrate. The objectives were to determine: (a) the effect of nitrate supply on N metabolism and growth and (b) the interrelationship between nitrate uptake, flux, and reduction on th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.6.1184
更新日期:1980-12-01 00:00:00
abstract::The existence of sphingolipid- and sterol-enriched microdomains, known as lipid rafts, in the plasma membrane (PM) of eukaryotic cells is well documented. To obtain more insight into the lipid molecular species required for the formation of microdomains in plants, we have isolated detergent (Triton X-100)-resistant me...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.091496
更新日期:2007-01-01 00:00:00
abstract::The effect of the exogenous and endogenous NO(3) (-) concentration on net uptake, influx, and efflux of NO(3) (-) and on nitrate reductase activity (NRA) in roots was studied in Phaseolus vulgaris L. cv. Witte Krombek. After exposure to NO(3) (-), an apparent induction period of about 6 hours occurred regardless of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.754
更新日期:1982-09-01 00:00:00
abstract::Rhizomes are organs of fundamental importance to plant competitiveness and invasiveness. We have identified genes expressed at substantially higher levels in rhizomes than other plant parts, and explored their functional categorization, genomic organization, regulatory motifs, and association with quantitative trait l...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082891
更新日期:2006-11-01 00:00:00
abstract::AGL15 (AGAMOUS-like 15), a member of the MADS-domain family of regulatory factors, accumulates preferentially in the organs and tissues derived from double fertilization in flowering plants (i.e. the embryo, suspensor, and endosperm). The developmental role of AGL15 is still undefined. If it is involved in embryogenes...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.1.121
更新日期:1999-05-01 00:00:00
abstract::A technique is presented for the isolation of vacuoles from Sedum telephium L. leaves. Leaf material is digested enzymically to produce protoplasts rapidly which are partially lysed by gentle osmotic shock and the inclusion of 5 millimolar ethyleneglycol-bis (beta-aminoethyl ether)N,N'-tetraacetic acid in the wash med...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.3.379
更新日期:1980-09-01 00:00:00
abstract::Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added. Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium. Little or no growth o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.5.598
更新日期:1970-05-01 00:00:00
abstract::Chloroplasts have been isolated from 4- to 6-day-old corn (Zea mays) leaves capable of assimilating 45 micromoles CO(2) per milligram chlorophyll per hour. The effects of various factors such as inorganic phosphate, reducing agents, inhibitors, intermediates of the photosynthetic carbon reduction cycle, organic acids,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.4.607
更新日期:1972-04-01 00:00:00
abstract::Excision of spinach (Spinacia oleracea L.) leaves had no effect on photosynthetic rates, but altered normal carbon partitioning to favor increased formation of starch and decreased formation of sucrose. The changes were evident within 2 hours after excision. Concurrently, leaf fructose-2,6-bisphosphate content increas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.2.445
更新日期:1984-02-01 00:00:00
abstract::Nitrogen-limited and nitrogen-sufficient cell cultures of Selenastrum minutum (Naeg.) Collins (Chlorophyta) were used to investigate the dependence of NH(4) (+) assimilation on exogenous CO(2). N-sufficient cells were only able to assimilate NH(4) (+) maximally in the presence of CO(2) and light. Inhibition of photosy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.1.192
更新日期:1991-01-01 00:00:00
abstract::The rhizophore of Selaginella willdenovii Baker develops from the ventral angle meristem. The morphological nature of this organ has been in dispute. The purpose of this investigation was to obtain physiological evidence to support the contention that the rhizophore is a root and not a shoot. This was accomplished by ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.5.738
更新日期:1974-05-01 00:00:00
abstract::The initial burst of respiratory activity (Q(o) (2)) of imbibing soybean (Glycine max [L.] Merr. var. Wayne) seed tissue is cyanide-insensitive, and sensitive to salicylhydroxamate: presumptive evidence for the presence of alternate respiration. The initial O(2) consumption is also highly sensitive to propyl gallate. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.3.470
更新日期:1978-09-01 00:00:00
abstract::When cyclic photophosphorylation is inhibited in Chlorella vulgaris cells by carbonylcyanide-trifluoromethoxy phenylhy-drazone, photosynthetic CO(2)-fixation under anaerobic conditions exhibits a distinct lag. Under the same conditions, the light-dependent formation of ribulose diphosphate shows also this lag. It is c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.5.825
更新日期:1973-05-01 00:00:00
abstract::In a study on 3-day maize (Zea mays) seedlings, grown on nitrate, requirements were established for the maximum extraction and optimum stabilization of nitrate reductase in vitro. With the primary root, 5 mm cysteine were required in the extraction medium, but for the scutellum, which has a high level of endogenous th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.3.191
更新日期:1973-09-01 00:00:00
abstract::A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.071589
更新日期:2005-12-01 00:00:00
abstract::We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogena...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.115246
更新日期:2008-05-01 00:00:00
abstract::The eukaryotic genome is duplicated exactly once per cell division cycle. A strategy that limits every replication origin to a single initiation event is tightly regulated by a multiprotein complex, which involves at least 20 protein factors. A key player in this regulation is the evolutionary conserved hexameric MCM2...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.074294
更新日期:2006-02-01 00:00:00
abstract::The ATP-dependence of the membrane potential of soybean (Glycine max L. cv Williams '79) roots was determined by correlating the decrease in ATP levels with the decrease in the membrane potential caused by carbonyl cyanide m-chlorophenylhydrazone (CCCP) or cyanide. The effects of the ATPase inhibitors, vanadate and fl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.1.1
更新日期:1984-05-01 00:00:00
abstract::Histone proteins play a central role in chromatin packaging, and modification of histones is associated with chromatin accessibility. SET domain [Su(var)3-9, Enhancer-of-zeste, Trithorax] proteins are one class of proteins that have been implicated in regulating gene expression through histone methylation. The relatio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.013722
更新日期:2003-06-01 00:00:00
abstract::The breakdown of sucrose to feed both hexoses into glycolytic carbon flow can occur by the sucrose synthase pathway. This uridine diphosphate (UDP) and pyrophosphate (PPi)-dependent pathway was biochemically characterized using soluble extracts from several plants. The sucrolysis process required the simultaneous pres...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.2.635
更新日期:1989-06-01 00:00:00
abstract::The light dependence of D1 phosphorylation is unique to higher plants, being constitutive in cyanobacteria and algae. In a photoautotrophic higher plant, Spirodela oligorrhiza, grown in greenhouse conditions under natural diurnal cycles of solar irradiation, the ratio of phosphorylated versus total D1 protein (D1-P in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013441
更新日期:2002-12-01 00:00:00
abstract::Syntheses are described for tetra and pentadeutero indole-3-acetic acid (IAA) labeled in positions 4, 5, 6, 7 or 2, 4, 5, 6, 7 of the indole moiety. Polydeuterated IAA is proposed as an internal standard for gas chromatographic-mass spectrometric analysis of IAA by selected ion monitoring. Nanogram amounts of IAA may ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.4.775
更新日期:1980-10-01 00:00:00
abstract::Alfalfa was grown hydroponically in 0, 0.6, and 4.8 millimolar K in order to determine the influence of tissue level of K on photosynthesis, dark respiration, photorespiration, stomatal and mesophyll resistance to CO(2), photosystem I and II activity, and synthesis and activity of ribulose 1,5-bisphosphate carboxylase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.5.878
更新日期:1979-05-01 00:00:00
abstract::Crude plasma membranes of corn (Zea mays L.) roots were obtained according to MI De Michelis and RM Spanswick (1986 Plant Physiol 81: 542-547). This preparation, which contained tightly sealed vesicles displaying Mg-ATP dependent H(+)-transport, was purified by phase partitioning. The percentage of inside-out vesicles...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.2.491
更新日期:1988-06-01 00:00:00
abstract::Side chains of sugar beet (Beta vulgaris) pectins, which are mainly composed of arabinose (Ara) and galactose (Gal) residues, are esterified by ferulic acid units. Enzymatic hydrolysis of beet cell walls yielded several feruloylated oligosaccharides, which were separated by hydrophobic interaction chromatography. Two ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.035311
更新日期:2004-03-01 00:00:00
abstract::Knowledge about physiological stress thresholds provides crucial information about plant performance and survival under drought. In this study, we report on the triphasic nature of the relationship between plant water potential (Ψ) at predawn and midday and describe a method that predicts Ψ at stomatal closure and tur...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00500
更新日期:2020-10-01 00:00:00
abstract::Photosynthetically active bundle sheath strands capable of assimilating up to 8 micromoles CO(2) per milligram chlorophyll per hour have been isolated from fully expanded leaves of Zea mays L. Mesophyll cell contamination of the preparations was negligible, as evidenced by light and electron microscopy and by a high r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.4.787
更新日期:1973-04-01 00:00:00