Slow-growth phenotype of transgenic tomato expressing apoplastic invertase.

Abstract:

:The growth of transgenic tomato (Lycopersicon esculentum) plants that express in their apoplast yeast invertase under the control of the cauliflower mosaic virus 35S promoter is severely inhibited. The higher the level of invertase, the greater the inhibition of growth. A second phenotypic characteristic of these transgenic plants is the development of yellow and necrotic spots on the leaves, and leaf curling. Again the severity of the symptoms is correlated with the level of invertase. These symptoms do not develop in shaded leaves indicating the need for photosynthesis. Keeping the plants in the dark for a prolonged period (24 hours) results in the disappearance of leaf starch from the control plants, but not from the plants with apoplastic invertase. These results are consistent with the interpretation that apoplastic invertase prevents photosynthate export from source leaves and that phloem loading includes an apoplastic step.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Dickinson CD,Altabella T,Chrispeels MJ

doi

10.1104/pp.95.2.420

subject

Has Abstract

pub_date

1991-02-01 00:00:00

pages

420-5

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

95

pub_type

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