Analysis of metabolic flux phenotypes for two Arabidopsis mutants with severe impairment in seed storage lipid synthesis.

abstract：:Increasing the yield of oilseed crops is an important objective for biotechnologists. A number of individual genes involved in triacylglycerol metabolism have previously been reported to enhance the oil content of seeds when their expression is altered. However, it has yet to be established whether specific combinatio...

journal_title：Plant physiology

authors： van Erp H,Kelly AA,Menard G,Eastmond PJ

更新日期：2014-05-01 00:00:00

abstract：:A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result ...

journal_title：Plant physiology

authors： Bölling C,Fiehn O

更新日期：2005-12-01 00:00:00

abstract：:Radioactive gibberellin A(5) ((3)H-GA(5)) was synthesized from gibberellic acid. When it was applied to dwarf peas grown in the dark, an average of 3% was converted to another acid gibberellin within 48 hours. The biological activity of the metabolite did not account for the response to applied GA(5). GA(5) is therefo...

journal_title：Plant physiology

authors： Musgrave A,Kende H

更新日期：1970-01-01 00:00:00

abstract：:Two benzoyl-l-tyrosine p-nitroanilide hydrolases (BTPAases I and II) were purified from the etiolated leaves of Zea mays L. and characterized. BTPAase I was electrophoretically homogeneous and consisted of two identical subunits having a molecular weight of 53,000. The molecular weight of BTPAase II was 65,000. The Mi...

journal_title：Plant physiology

authors： Doi M,Shioi Y

更新日期：1987-07-01 00:00:00

abstract：:Fluxes of mineral nutrients in the xylem are strongly influenced by interactions with the surrounding stem tissues and are probably regulated by them. Toward a mechanistic understanding of these interactions, we applied stable isotope tracers of magnesium, potassium, and calcium continuously to the transpiration strea...

journal_title：Plant physiology

authors： Metzner R,Schneider HU,Breuer U,Thorpe MR,Schurr U,Schroeder WH

更新日期：2010-02-01 00:00:00

abstract：:The 7S seed storage protein (beta-conglycinin) of soybean (Glycine max [L]. Merr.) has three major subunits; alpha, alpha', and beta. Accumulation of the beta-subunit, but not the alpha- and alpha'-subunits, has been shown to be repressed by exogenously applied methionine to the immature cotyledon culture system (LP H...

journal_title：Plant physiology

authors： Fujiwara T,Hirai MY,Chino M,Komeda Y,Naito S

更新日期：1992-05-01 00:00:00

abstract：:The Craig-Gordon evaporative enrichment model of the hydrogen (deltaD) and oxygen (delta(18)O) isotopes of water was tested in a controlled-environment gas exchange cuvette over a wide range (400 per thousand deltaD and 40 per thousand delta(18)O) of leaf waters. (Throughout this paper we use the term "leaf water" to ...

journal_title：Plant physiology

authors： Roden JS,Ehleringer JR

更新日期：1999-08-01 00:00:00

abstract：:Elevated carbon dioxide (CO2) in leaves closes stomatal apertures. Research has shown key functions of the β-carbonic anhydrases (βCA1 and βCA4) in rapid CO2-induced stomatal movements by catalytic transmission of the CO2 signal in guard cells. However, the underlying mechanisms remain unclear, because initial studies...

journal_title：Plant physiology

authors： Hu H,Rappel WJ,Occhipinti R,Ries A,Böhmer M,You L,Xiao C,Engineer CB,Boron WF,Schroeder JI

更新日期：2015-10-01 00:00:00

abstract：:Carotenoids are essential pigments of the photosynthetic apparatus and an indispensable component of the human diet. In addition to being potent antioxidants, they also provide the vitamin A precursor beta-carotene. In tomato (Solanum lycopersicum) fruits, carotenoids accumulate in specialized plastids, the chromoplas...

journal_title：Plant physiology

authors： Apel W,Bock R

更新日期：2009-09-01 00:00:00

abstract：:We functionally characterized the grape (Vitis vinifera) VvPIP2;4N (for Plasma membrane Intrinsic Protein) aquaporin gene. Expression of VvPIP2;4N in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that VvPIP2;...

journal_title：Plant physiology

authors： Perrone I,Gambino G,Chitarra W,Vitali M,Pagliarani C,Riccomagno N,Balestrini R,Kaldenhoff R,Uehlein N,Gribaudo I,Schubert A,Lovisolo C

更新日期：2012-10-01 00:00:00

abstract：:The Arabidopsis type II peroxiredoxin (PRXII) family is composed of six different genes, five of which are expressed. On the basis of the nucleotide and protein sequences, we were able to define three subgroups among the PRXII family. The first subgroup is composed of AtPRXII-B, -C, and -D, which are highly similar an...

journal_title：Plant physiology

authors： Bréhélin C,Meyer EH,de Souris JP,Bonnard G,Meyer Y

更新日期：2003-08-01 00:00:00

abstract：:Legume-rhizobia symbiosis is a time-limited process due to the onset of senescence, which results in the degradation of host plant cells and symbiosomes. A number of transcription factors, proteases, and functional genes have been associated with nodule senescence; however, whether other proteases or transcription fac...

journal_title：Plant physiology

authors： Deng J,Zhu F,Liu J,Zhao Y,Wen J,Wang T,Dong J

更新日期：2019-12-01 00:00:00

abstract：:Root size and architecture are important crop plant traits, as they determine access to water and soil nutrients. The plant hormone cytokinin is a negative regulator of root growth and branching. Here, we generated transgenic barley (Hordeum vulgare) plants with an enlarged root system by enhancing cytokinin degradati...

journal_title：Plant physiology

authors： Ramireddy E,Hosseini SA,Eggert K,Gillandt S,Gnad H,von Wirén N,Schmülling T

更新日期：2018-07-01 00:00:00

abstract：:The particulate enzyme fraction from mung bean (Phaseolus aureus) seedlings catalyzes the incorporation of mannose from GDP-[(14)C]mannose into mannosyl-phosphoryl-dolichol and of N-acetylglucosamine from UDP-[(3)H]N-acetylglucosamine into N-acetylglucosamine-pyrophosphoryl-polyisoprenol. Bacitracin inhibits the trans...

journal_title：Plant physiology

authors： Ericson MC,Gafford J,Elbein AD

更新日期：1978-09-01 00:00:00

abstract：:The effect of atmospheric methyl jasmonate on the oxylipin pathway was investigated in leaves of tobacco (Nicotiana tabacum L.), cucumber (Cucumis sativa L.), and Arabidopsis thaliana (L.). Differential sensitivities of test plants to methyl jasmonate were observed. Thus, different concentrations of methyl jasmonate w...

journal_title：Plant physiology

authors： Avdiushko S,Croft KP,Brown GC,Jackson DM,Hamilton-Kemp TR,Hildebrand D

更新日期：1995-12-01 00:00:00

abstract：:Triacontanol applied to IR-8 rice (Oryza sativa L.) seedlings in nutrient solution caused an increase in dry weight during a 6-hour dark period. This increase was altered by atmospheric CO(2) and O(2) concentrations. The largest growth response occurred from 200 to 350 muliters/liter CO(2) with 5% O(2). The treated se...

journal_title：Plant physiology

authors： Bittenbender HC,Dilley DR,Wert V,Ries SK

更新日期：1978-05-01 00:00:00

abstract：:We have analyzed gene regulation of the Lhc supergene family in poplar (Populus spp.) and Arabidopsis (Arabidopsis thaliana) using digital expression profiling. Multivariate analysis of the tissue-specific, environmental, and developmental Lhc expression patterns in Arabidopsis and poplar was employed to characterize ...

journal_title：Plant physiology

authors： Klimmek F,Sjödin A,Noutsos C,Leister D,Jansson S

更新日期：2006-03-01 00:00:00

abstract：:Histone H2B monoubiquitination (H2Bub) is being recognized as a regulatory mechanism that controls a range of cellular processes in plants, but the molecular mechanisms of H2Bub that are involved in responses to biotic stress are largely unknown. In this study, we used wild-type and H2Bub loss-of-function mutations of...

journal_title：Plant physiology

authors： Hu M,Pei BL,Zhang LF,Li YZ

更新日期：2014-04-01 00:00:00

abstract：:Bicarbonate uptake by isolated chloroplast envelope membranes and intact chloroplasts of spinach (Spinacia oleracea L. var. Viroflay) in darkness exhibited a similar dependency upon temperature, pH, time, and concentrations of isolated or attached envelope membranes. This similarity in uptake properties demonstrates t...

journal_title：Plant physiology

authors： Poincelot RP

更新日期：1974-10-01 00:00:00

abstract：:Demand-driven signaling will contribute to regulation of sulfur acquisition and distribution within the plant. To investigate the regulatory mechanisms pedospheric sulfate and atmospheric H(2)S supply were manipulated in Brassica oleracea. Sulfate deprivation of B. oleracea seedlings induced a rapid increase of the su...

journal_title：Plant physiology

authors： Buchner P,Stuiver CE,Westerman S,Wirtz M,Hell R,Hawkesford MJ,De Kok LJ

更新日期：2004-10-01 00:00:00

abstract：:Experiments with ethylene-insensitive tomato (Lycopersicon esculentum) and petunia (Petunia x hybrida) plants were conducted to determine if normal or adventitious root formation is affected by ethylene insensitivity. Ethylene-insensitive Never ripe (NR) tomato plants produced more below-ground root mass but fewer abo...

journal_title：Plant physiology

authors： Clark DG,Gubrium EK,Barrett JE,Nell TA,Klee HJ

更新日期：1999-09-01 00:00:00

abstract：:Sorghum (Sorghum bicolor L. Moench) accumulates the anthocyanin cyanidin 3-dimalonyl glucoside in etiolated mesocotyls in response to light. Inoculation with the nonpathogenic fungus Cochliobolus heterostrophus drastically reduced the light-induced accumulation of anthocyanin by repressing the transcription of the ant...

journal_title：Plant physiology

authors： Lo SC,Nicholson RL

更新日期：1998-03-01 00:00:00

abstract：:The unicellular green alga Chlorella kessleri can induce monosaccharide-H+ symport catalyzing the energy-dependent transport of D-glucose (D-Glc) and several other pentoses and hexoses across the plasmalemma. The gene coding for the inducible HUP1 monosaccharide-H+ symporter has been cloned and the protein has been ch...

journal_title：Plant physiology

authors： Stadler R,Wolf K,Hilgarth C,Tanner W,Sauer N

更新日期：1995-01-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) are fundamental components of the plant innate immune system. MPK3 and MPK6 are Arabidopsis (Arabidopsis thaliana) MAPKs activated by pathogens and elicitors such as oligogalacturonides (OGs), which function as damage-associated molecular patterns, and flg22, a well-known micr...

journal_title：Plant physiology

authors： Galletti R,Ferrari S,De Lorenzo G

更新日期：2011-10-01 00:00:00

abstract：:Four putative apyrase genes were identified from the model legume Medicago truncatula. Two of the genes identified from M. truncatula (Mtapy1 and Mtapy4) are expressed in roots and are inducible within 3 h after inoculation with Sinorhizobium meliloti. The level of mRNA expression of the other two putative apyrases, M...

journal_title：Plant physiology

authors： Cohn JR,Uhm T,Ramu S,Nam YW,Kim DJ,Penmetsa RV,Wood TC,Denny RL,Young ND,Cook DR,Stacey G

更新日期：2001-04-01 00:00:00

abstract：:Protein bodies were prepared from the cotyledons of pumpkin (Cucurbita sp.) seeds by employing a nonaqueous isolation method. Both light micrographic examination and the marker enzyme assays have shown that the isolated protein bodies were intact and contamination with other cell organelles or cytoplasmic components w...

journal_title：Plant physiology

authors： Hara-Nishimura I,Nishimura M,Matsubara H,Akazawa T

更新日期：1982-09-01 00:00:00

abstract：:The cloning and analysis of two different cDNA clones encoding putative maize (Zea mays L.) chitinases obtained by polymerase chain reaction (PCR) and cDNA library screening is described. The cDNA library was made from poly(A)+ RNA from leaves challenged with mercuric chloride for 2 d. The two clones, pCh2 and pCh11, ...

journal_title：Plant physiology

authors： Wu S,Kriz AL,Widholm JM

更新日期：1994-08-01 00:00:00

abstract：:Treatment of grass leaves with either a purified pectin lyase of Aspergillus japonicus or a purified xylanase of Trichoderma viride could lead to the isolation of some single leaf cells. However, a mixture of pectin lyase and xylanase brought about more rapid isolation of single cells than did either of the two enzyme...

journal_title：Plant physiology

authors： Ishii S

更新日期：1984-12-01 00:00:00

abstract：:The long-day plant Arabidopsis thaliana (L.) Heynh. flowers early in response to brief end-of-day (EOD) exposures to far-red light (FR) following a fluorescent short day of 8 h. FR promotion of flowering was nullified by subsequent brief red light (R) EOD exposure, indicating phytochrome involvement. The EOD response ...

journal_title：Plant physiology

authors： Bagnall DJ,King RW,Whitelam GC,Boylan MT,Wagner D,Quail PH

更新日期：1995-08-01 00:00:00

abstract：:Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate (I-1,2,3,4,5,6-P6). A number of phytases with varying specificities, properties, and localizations hydrolyze phytic acid present in cells. The specificity of hydrolysis of phytic acid by alkaline p...

journal_title：Plant physiology

authors： Barrientos L,Scott JJ,Murthy PP

更新日期：1994-12-01 00:00:00