Differential regulation of a family of apyrase genes from Medicago truncatula.

Abstract:

:Four putative apyrase genes were identified from the model legume Medicago truncatula. Two of the genes identified from M. truncatula (Mtapy1 and Mtapy4) are expressed in roots and are inducible within 3 h after inoculation with Sinorhizobium meliloti. The level of mRNA expression of the other two putative apyrases, Mtapy2 and Mtapy3, was unaffected by rhizobial inoculation. Screening of a bacterial artificial chromosome library of M. truncatula genomic DNA showed that Mtapy1, Mtapy3, and Mtapy4 are present on a single bacterial artificial chromosome clone. This apyrase cluster was mapped to linkage group seven. A syntenic region on soybean linkage group J was found to contain at least two apyrase genes. Screening of nodulation deficient mutants of M. truncatula revealed that two such mutants do not express apyrases to any detectable level. The data suggest a role for apyrases early in the nodulation response before the involvement of root cortical cell division leading to the nodule structure.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Cohn JR,Uhm T,Ramu S,Nam YW,Kim DJ,Penmetsa RV,Wood TC,Denny RL,Young ND,Cook DR,Stacey G

doi

10.1104/pp.125.4.2104

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

2104-19

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

125

pub_type

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