Comparing the in vivo function of α-carboxysomes and β-carboxysomes in two model cyanobacteria.

Abstract:

:The carbon dioxide (CO2)-concentrating mechanism of cyanobacteria is characterized by the occurrence of Rubisco-containing microcompartments called carboxysomes within cells. The encapsulation of Rubisco allows for high-CO2 concentrations at the site of fixation, providing an advantage in low-CO2 environments. Cyanobacteria with Form-IA Rubisco contain α-carboxysomes, and cyanobacteria with Form-IB Rubisco contain β-carboxysomes. The two carboxysome types have arisen through convergent evolution, and α-cyanobacteria and β-cyanobacteria occupy different ecological niches. Here, we present, to our knowledge, the first direct comparison of the carboxysome function from α-cyanobacteria (Cyanobium spp. PCC7001) and β-cyanobacteria (Synechococcus spp. PCC7942) with similar inorganic carbon (Ci; as CO2 and HCO3-) transporter systems. Despite evolutionary and structural differences between α-carboxysomes and β-carboxysomes, we found that the two strains are remarkably similar in many physiological parameters, particularly the response of photosynthesis to light and external Ci and their modulation of internal ribulose-1,5-bisphosphate, phosphoglycerate, and Ci pools when grown under comparable conditions. In addition, the different Rubisco forms present in each carboxysome had almost identical kinetic parameters. The conclusions indicate that the possession of different carboxysome types does not significantly influence the physiological function of these species and that similar carboxysome function may be possessed by each carboxysome type. Interestingly, both carboxysome types showed a response to cytosolic Ci, which is of higher affinity than predicted by current models, being saturated by 5 to 15 mm Ci. This finding has bearing on the viability of transplanting functional carboxysomes into the C3 chloroplast.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Whitehead L,Long BM,Price GD,Badger MR

doi

10.1104/pp.114.237941

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

398-411

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.114.237941

journal_volume

165

pub_type

杂志文章
  • Arabidopsis Pht1;5 mobilizes phosphate between source and sink organs and influences the interaction between phosphate homeostasis and ethylene signaling.

    abstract::Phosphorus (P) remobilization in plants is required for continuous growth and development. The Arabidopsis (Arabidopsis thaliana) inorganic phosphate (Pi) transporter Pht1;5 has been implicated in mobilizing stored Pi out of older leaves. In this study, we used a reverse genetics approach to study the role of Pht1;5 i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.174805

    authors: Nagarajan VK,Jain A,Poling MD,Lewis AJ,Raghothama KG,Smith AP

    更新日期:2011-07-01 00:00:00

  • Properties of Phosphoglycolate Phosphatase from Chlamydomonas reinhardtii and Anacystis nidulans.

    abstract::The levels of activity of 2-phosphoglycolate phosphatase in the green algae, Chlamydomonas reinhardtii and Chlorella vulgaris, were in the range of 37 to 60 micromoles per milligram chlorophyll per hour and in the blue-green algae, Anacystis nidulans and Anabaena variabilis were 204 to 310 micromoles per milligram chl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.2.394

    authors: Husic HD,Tolbert NE

    更新日期:1985-10-01 00:00:00

  • Role and interrelationship of Gα protein, hydrogen peroxide, and nitric oxide in ultraviolet B-induced stomatal closure in Arabidopsis leaves.

    abstract::Heterotrimeric G proteins have been shown to transmit ultraviolet B (UV-B) signals in mammalian cells, but whether they also transmit UV-B signals in plant cells is not clear. In this paper, we report that 0.5 W m(-2) UV-B induces stomatal closure in Arabidopsis (Arabidopsis thaliana) by eliciting a cascade of intrace...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.211623

    authors: He JM,Ma XG,Zhang Y,Sun TF,Xu FF,Chen YP,Liu X,Yue M

    更新日期:2013-03-01 00:00:00

  • The relationship of the peroxidative indoleacetic Acid oxidase system to in vivo ethylene synthesis in cotton.

    abstract::Since peroxidase and manganese have been implicated in both auxin destruction and ethylene production, the effect of auxins and high tissue levels of manganese on the peroxidative indoleacetic acid oxidase system and the internal level of ethylene was determined in cotton (Gossypium hirsutum L. cv. Watson GL-7). The h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.4.555

    authors: Fowler JL,Morgan PW

    更新日期:1972-04-01 00:00:00

  • Transport of rubidium absorbed by the apex of the bean root.

    abstract::Rubidium-86 was applied to the terminal 5.0 mm region of intact roots for a 6 hour period. The plants were then harvested after 0, 18, 24, 48, 72, or 96 hours additional growth in a rubidium-free continuously renewed nutrient solution. A substantial portion of the rubidium absorbed in a region of the root devoid of fu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.4.583

    authors: Steward KK,Koontz HV

    更新日期:1968-04-01 00:00:00

  • Interactions between Mild NaCl Stress and Red Light during Lettuce (Lactuca sativa L. cv Grand Rapids) Seed Germination.

    abstract::The sensitivity of lettuce (Lactuca sativa L. cv Grand Rapids) seeds to red light was reduced by NaCl concentrations which had no effect upon the germination of continuously illuminated seeds. The germination capacity of the seeds was fully restored by increased red light exposures. Indirect evidence indicates that Na...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.1.149

    authors: Scorer KN,Epel BL,Waisel Y

    更新日期:1985-09-01 00:00:00

  • Identification and gene expression of anaerobically induced enolase in Echinochloa phyllopogon and Echinochloa crus-pavonis.

    abstract::Enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11) has been identified as an anaerobic stress protein in Echinochloa oryzoides based on the homology of its internal amino acid sequence with those of enolases from other organisms, by immunological reactivity, and induction of catalytic activity during anaerobic str...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.2.433

    authors: Fox TC,Mujer CV,Andrews DL,Williams AS,Cobb BG,Kennedy RA,Rumpho ME

    更新日期:1995-10-01 00:00:00

  • Water Transport across Maize Roots : Simultaneous Measurement of Flows at the Cell and Root Level by Double Pressure Probe Technique.

    abstract::A double pressure probe technique was used to measure simultaneously water flows and hydraulic parameters of individual cells and of excised roots of young seedlings of maize (Zea mays L.) in osmotic experiments. By following initial flows of water at the cell and root level and by estimating the profiles of driving f...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.1.305

    authors: Zhu GL,Steudle E

    更新日期:1991-01-01 00:00:00

  • A leaky mutation in DWARF4 reveals an antagonistic role of brassinosteroid in the inhibition of root growth by jasmonate in Arabidopsis.

    abstract::The F-box protein CORONATINE INSENSITIVE1 (COI1) plays a central role in jasmonate (JA) signaling and is required for all JA responses in Arabidopsis (Arabidopsis thaliana). To dissect JA signal transduction, we isolated the partially suppressing coi1 (psc1) mutant, which partially suppressed coi1 insensitivity to JA ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.140202

    authors: Ren C,Han C,Peng W,Huang Y,Peng Z,Xiong X,Zhu Q,Gao B,Xie D

    更新日期:2009-11-01 00:00:00

  • Antisense suppression of 2-cysteine peroxiredoxin in Arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism.

    abstract::The aim of this study was to characterize the effect of decreased 2-cysteine peroxiredoxin (2-CP) on the leaf anti-oxidative system in Arabidopsis. At three stages of leaf development, two lines of transgenic Arabidopsis mutants with decreased contents of chloroplast 2-CP were compared with wild type and a control lin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.2.823

    authors: Baier M,Noctor G,Foyer CH,Dietz KJ

    更新日期:2000-10-01 00:00:00

  • Involvement of the histone acetyltransferase AtHAC1 in the regulation of flowering time via repression of FLOWERING LOCUS C in Arabidopsis.

    abstract::Histone acetylation is an important posttranslational modification correlated with gene activation. In Arabidopsis (Arabidopsis thaliana), the histone acetyltransferase AtHAC1 is homologous to animal p300/CREB (cAMP-responsive element-binding protein)-binding proteins, which are the main histone acetyltransferases par...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.095521

    authors: Deng W,Liu C,Pei Y,Deng X,Niu L,Cao X

    更新日期:2007-04-01 00:00:00

  • Inhibition of the Hill Reaction by Tris and Restoration by Electron Donation to Photosystem II.

    abstract::Experiments in which chloroplasts were washed with tris and tricine buffers at different pH's indicated that the non-protonated (uncharged) form of tris was inhibitory to the Hill reaction while the protonated form of tris and the zwitterionic forms of tricine were non-inhibitory. Buffers analogous to tris and tricine...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.3.435

    authors: Yamashita T,Butler WL

    更新日期:1969-03-01 00:00:00

  • Changes in Esterification of the Uronic Acid Groups of Cell Wall Polysaccharides during Elongation of Maize Coleoptiles.

    abstract::Cell walls of grasses have two major polysaccharides that contain uronic acids, the hemicellulosic glucuronoarabinoxylans and the galactosyluronic acid-rich pectins. A technique whereby esterified uronic acid carboxyl groups are reduced selectively to yield their respective 6,6-dideuterio neutral sugars was used to de...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.2.646

    authors: Kim JB,Carpita NC

    更新日期:1992-02-01 00:00:00

  • Metabolism of Indole-3-Acetic Acid: III. Identification of Metabolites Isolated from Crown Gall Callus Tissue.

    abstract::The metabolism of labeled indole-3-acetic acid (IAA-2-(14)C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were asp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.5.666

    authors: Feung CS,Hamilton RH,Mumma RO

    更新日期:1976-11-01 00:00:00

  • Observations of hydrogen and oxygen isotopes in leaf water confirm the craig-gordon model under wide-ranging environmental conditions

    abstract::The Craig-Gordon evaporative enrichment model of the hydrogen (deltaD) and oxygen (delta(18)O) isotopes of water was tested in a controlled-environment gas exchange cuvette over a wide range (400 per thousand deltaD and 40 per thousand delta(18)O) of leaf waters. (Throughout this paper we use the term "leaf water" to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.4.1165

    authors: Roden JS,Ehleringer JR

    更新日期:1999-08-01 00:00:00

  • Red clover HCT2, a hydroxycinnamoyl-coenzyme A:malate hydroxycinnamoyl transferase, plays a crucial role in biosynthesis of phaselic acid and other hydroxycinnamoyl-malate esters in vivo.

    abstract::In red clover (Trifolium pratense) leaves, phaselic acid (2-O-caffeoyl-L-malate) accumulates to several mmol kg(-1) fresh weight and is a crucial component of a natural system that prevents protein breakdown during harvest and storage of this forage crop. Previously, we identified HCT2, a red clover gene encoding a hy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.166793

    authors: Sullivan ML,Zarnowski R

    更新日期:2011-03-01 00:00:00

  • Variable Chlorophyll a Fluorescence and CO(2) Uptake in Water-Stressed White Spruce Seedlings.

    abstract::Regulation of photosynthetic activity can contribute to the prevention of photodamage in stress resistant plants during exposure to drought or low temperatures. Responses to increasing levels of water stress were examined in seedlings of the stress resistant forest conifer, white spruce (Picea glauca [Moench.] Voss). ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.3.744

    authors: Toivonen P,Vidaver W

    更新日期:1988-03-01 00:00:00

  • Dehydroascorbate influences the plant cell cycle through a glutathione-independent reduction mechanism.

    abstract::Glutathione is generally accepted as the principal electron donor for dehydroascorbate (DHA) reduction. Moreover, both glutathione and DHA affect cell cycle progression in plant cells. But other mechanisms for DHA reduction have been proposed. To investigate the connection between DHA and glutathione, we have evaluate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.033548

    authors: Potters G,Horemans N,Bellone S,Caubergs RJ,Trost P,Guisez Y,Asard H

    更新日期:2004-04-01 00:00:00

  • Iron-sulfur centers and activities of the photosynthetic electron transport chain in iron-deficient cultures of the blue-green alga aphanocapsa.

    abstract::Cultures of the blue-green alga, Aphanocapsa, were grown under iron-limiting conditions and changes in concentration of redox components of the photosynthetic electron transport chain, particularly iron-sulfur centers, were monitored by spectroscopic methods. A moderate iron depletion (1/10 of the normal concentration...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.724

    authors: Sandmann G,Malkin R

    更新日期:1983-11-01 00:00:00

  • Thermal effect of CO(2) on apoplastic ice in rye and oat during freezing.

    abstract::Meristematic tissues from rye (Secale cereale) and oat (Avena sativa) were studied in an isothermal calorimeter at -3 degrees C. When the frozen tissue was placed in the calorimeter, the pressure increased within 4 d to 25 and 9 kPa above ambient pressure in the sample vessels containing crowns of rye and oat, respect...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.861

    authors: Livingston DP 3rd,Olien CR,Premakumar R

    更新日期:2000-03-01 00:00:00

  • A Variation of C(4) Leaf Anatomy in Arundinella hirta (Gramineae).

    abstract::The species Arundinella hirta L. posseses a striking variation of the leaf anatomy that is characteristic of C(4) grasses. In addition to a sheath of large, bright green cells around the vascular bundles, there are strands of large parenchyma cells which appear identical to the bundle sheath cells and which run parall...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.5.397

    authors: Crookston RK,Moss DN

    更新日期:1973-11-01 00:00:00

  • LEPS2, a phosphorus starvation-induced novel acid phosphatase from tomato.

    abstract::Phosphate (Pi) is one of the least available plant nutrients found in the soil. A significant amount of phosphate is bound in organic forms in the rhizosphere. Phosphatases produced by plants and microbes are presumed to convert organic phosphorus into available Pi, which is absorbed by plants. In this study we descri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.2.728

    authors: Baldwin JC,Karthikeyan AS,Raghothama KG

    更新日期:2001-02-01 00:00:00

  • Oleate desaturation in young winter wheat root tissue.

    abstract::[1,2-(14)C]Acetate was incorporated into the lipids of young wheat (Triticum aestivum L. cv Kharkov 22 MC) root tissue, but predominantly into sterols. [1-(14)C]Ammonium oleate was initially incorporated mainly into phosphatidylcholine (PC), and later into triglycerides (TGs). Diglycerides (DGs) contained 16% of the l...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1526

    authors: Willemot C,Labrecque J

    更新日期:1982-11-01 00:00:00

  • Effects of inorganic phosphate on the light dependent thylakoid energization of intact spinach chloroplasts.

    abstract::The light dependent energization of the thylakoid membrane was analyzed in isolated intact spinach (Spinacia oleracea L.) chloroplasts incubated with different concentrations of inorganic phosphate (Pi). Two independent methods were used: (a) the accumulation of [(14)C]5,5-dimethyl-2,4-oxazolidinedione and [(14)C] met...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.1.221

    authors: Heineke D,Stitt M,Heldt HW

    更新日期:1989-09-01 00:00:00

  • Relative insensitivity of mitochondria in hardened and nonhardened rye coleoptile cells to freezing in situ.

    abstract::Mitochondria were isolated from excised coleoptiles of hardened and nonhardened winter rye (Secale cereale L. cv. Puma) seedlings which had been frozen extracellularly to different temperatures. No significant differences in the respiratory functions (ADP/O and respiratory control) were observed between mitochondria i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.5.713

    authors: Singh J,de la Roche AI,Siminovitch D

    更新日期:1977-11-01 00:00:00

  • Developmental Loss of Photosystem II Activity and Structure in a Chloroplast-Encoded Tobacco Mutant, Lutescens-1.

    abstract::Lutescens-1, a tobacco mutant with a maternally inherited dysfunction, displayed an unusual developmental phenotype. In vivo measurement of chlorophyll fluorescence revealed deterioration in photosystem II (PSII) function as leaves expanded. Analysis of thylakoid membrane proteins by polyacrylamide gel electrophoresis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.1.19

    authors: Chia CP,Duesing JH,Arntzen CJ

    更新日期:1986-09-01 00:00:00

  • N and C NMR determination of methionine metabolism in developing soybean cotyledons.

    abstract::The metabolism of d- and l-methionine by immature cotyledons of soybean (Glycine max, L. cv Elf) grown in culture has been investigated using solid-state (13)C and (15)N nuclear magnetic resonance. d-Methionine is taken up by the cotyledons and converted to an amide, most likely by N-malonylation. About 16% of the l-m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.3.698

    authors: Coker GT,Garbow JR,Schaefer J

    更新日期:1987-03-01 00:00:00

  • Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids.

    abstract::Hydroxamic acids, R-CONHOH, are inhibitors specific to the respiratory pathway through the alternate, cyanide-insensitive terminal oxidase of plant mitochondria. The nature of the R group in these compounds affects the concentration at which the hydroxamic acids are effective, but it appears that all hydroxamic acids ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.1.124

    authors: Schonbaum GR,Bonner WD Jr,Storey BT,Bahr JT

    更新日期:1971-01-01 00:00:00

  • Genome-Wide Analysis of Yield in Europe: Allelic Effects Vary with Drought and Heat Scenarios.

    abstract::Assessing the genetic variability of plant performance under heat and drought scenarios can contribute to reduce the negative effects of climate change. We propose here an approach that consisted of (1) clustering time courses of environmental variables simulated by a crop model in current (35 years × 55 sites) and fu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00621

    authors: Millet EJ,Welcker C,Kruijer W,Negro S,Coupel-Ledru A,Nicolas SD,Laborde J,Bauland C,Praud S,Ranc N,Presterl T,Tuberosa R,Bedo Z,Draye X,Usadel B,Charcosset A,Van Eeuwijk F,Tardieu F

    更新日期:2016-10-01 00:00:00

  • Chloroplast thylakoid protein phosphatase is a membrane surface-associated activity.

    abstract::Chloroplast thylakoid protein phosphatase activity was measured using (32)P-labeled histone as an exogenous substrate and an assay of the (32)Pi released involving formation of a phosphomolybdate complex and organic extraction. The activity was liberated from wheat (Triticum aestivum) thylakoids by washing the membran...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.1.238

    authors: Sun G,Bailey D,Jones MW,Markwell J

    更新日期:1989-01-01 00:00:00