Phosphatidylinositol synthesis in castor bean endosperm: cytidine diphosphate diglyceride:inositol transferase.

Abstract:

:CDP-diglyceride:inositol transferase in endoplasmic reticulum fractions from castor bean (Ricinus communis) endosperm was partially characterized. The enzyme had a pH optimum of 8.5 and required Mn(2+) for activity. Maximal activity was at 1.5 millimolar MnCl(2). A K(m) of 0.30 mM was calculated for myo-inositol and 1.35 millimolar was estimated for CDP-dipalmitoylglyceride. Concentrations of CDP-dipalmitoylglyceride above 1.2 millimolar inhibited the enzyme. A deoxycholate concentration of 0.1% (w/v) stimulated the reaction slightly while Triton X-100 inhibited at all concentrations tested. Some incorporation of myo-inositol into phosphatidylinositol occurred in the absence of CDP-diglyceride.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Sexton JC,Moore TS

doi

10.1104/pp.62.6.978

subject

Has Abstract

pub_date

1978-12-01 00:00:00

pages

978-80

issue

6

eissn

0032-0889

issn

1532-2548

journal_volume

62

pub_type

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