Abstract:
:The molecular weight and isoelectric point of the plasma membrane H(+)-ATPase from red beet storage tissue were determined using N,N'-dicyclohexylcarbodiimide (DCCD) and a H(+)-ATPase antibody. When plasma membrane vesicles were incubated with 20 micromolar [(14)C]-DCCD at 0 degrees C, a single 97,000 dalton protein was visualized on a fluorograph of a sodium dodecyl sulfate polyacrylamide gel. A close correlation between [(14)C]DCCD labeling of the 97,000 dalton protein and the extent of ATPase inhibition over a range of DCCD concentration suggests that this 97,000 dalton protein is a component of the plasma membrane H(+)-ATPase. An antibody raised against the plasma membrane H(+)-ATPase of Neurospora crassa cross-reacted with the 97,000 dalton DCCD-binding protein, further supporting the identity of this protein. Immunoblots of two-dimensional gels of red beet plasma membrane vesicles indicated the isoelectric point of the H(+)-ATPase to be 6.5.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Oleski NA,Bennett ABdoi
10.1104/pp.83.3.569subject
Has Abstractpub_date
1987-03-01 00:00:00pages
569-72issue
3eissn
0032-0889issn
1532-2548journal_volume
83pub_type
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