Necessary conditions for isolation of tightly coupled higher plant mitochondria.

abstract：:To improve seed iron (Fe) content and bioavailability, it is crucial to decipher the mechanisms that control Fe storage during seed development. In Arabidopsis (Arabidopsis thaliana) seeds, most Fe is concentrated in insoluble precipitates, with phytate in the vacuoles of cells surrounding the vasculature of the embry...

journal_title：Plant physiology

authors： Mary V,Schnell Ramos M,Gillet C,Socha AL,Giraudat J,Agorio A,Merlot S,Clairet C,Kim SA,Punshon T,Guerinot ML,Thomine S

更新日期：2015-09-01 00:00:00

abstract：:A selected strain of wild oat (Avena fatua L.) seed has been shown to lose dormancy rapidly during moist soil incubation at 25 C, whereas seed kept similarly at 5 C maintained a high level of dormancy.The activities of cytosolic dehydrogenase enzymes of the pentose phosphate pathway were assayed throughout a period of...

journal_title：Plant physiology

authors： Adkins SW,Ross JD

更新日期：1981-07-01 00:00:00

abstract：:Light-dependent formation of ribulose-1,5 diphosphate is completely inhibited by low concentrations of 3-(3,4-dichlorophenyl)-1,1-dimethylurea which do not severely affect cyclic photophosphorylation. Also in Scenedesmus mutant number 11, capable of cyclic photophosphorylation, cellular ribulose-1,5 diphosphate-levels...

journal_title：Plant physiology

authors： Klob W,Kandler O,Tanner W

更新日期：1972-06-01 00:00:00

abstract：:Extracts of the tendrils of Pisum sativum, Var. Alaska, exhibit adenosine triphosphatase activity which is inversely proportional to the amount the tendrils have coiled. The specific viscosity of the extract decreases when ATP is added. This evidence indicates a possible role of a contractile adenosine triphosphatase ...

journal_title：Plant physiology

authors： Jaffe MJ,Galston AW

更新日期：1967-06-01 00:00:00

abstract：:The response of the membrane potential to HCO(3) (-) supply has been studied in the cyanobacterium Anabaena variabilis strain M-3 under various conditions. Changes in potential were followed with the aid of the lipophilic cation tetraphenyl phosphonium bromide.Addition of HCO(3) (-) to CO(2)-depleted cells resulted in...

journal_title：Plant physiology

authors： Kaplan A,Zenvirth D,Reinhold L,Berry JA

更新日期：1982-04-01 00:00:00

abstract：:The mechanism of sucrose transport was investigated in plasma membrane (PM) vesicles isolated from spinach (Spinacia oleracea L.) leaves. PM vesicles were isolated by aqueous two-phase partitioning and were equilibrated in pH 7.8 buffer containing K(+). The vesicles rapidly accumulated sucrose in the presence of a tra...

journal_title：Plant physiology

authors： Slone JH,Buckhout TJ,Vanderwoude WJ

更新日期：1991-06-01 00:00:00

abstract：:With the aim to specifically study the molecular mechanisms behind photoinhibition of photosystem I, stacked spinach (Spinacia oleracea) thylakoids were irradiated at 4 degrees C with far-red light (>715 nm) exciting photosystem I, but not photosystem II. Selective excitation of photosystem I by far-red light for 130 ...

journal_title：Plant physiology

authors： Tjus SE,Scheller HV,Andersson B,Møller BL

更新日期：2001-04-01 00:00:00

abstract：:Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the starchy endosperm protein of rice (Oryza sativa L. Japonica cv Koshihikari) during seed development confirmed that storage protein begins to accumulate about 5 days after flowering. Two polypeptide groups, 22 to 23 and 37 to 39 kilodaltons, the ...

journal_title：Plant physiology

authors： Yamagata H,Sugimoto T,Tanaka K,Kasai Z

更新日期：1982-10-01 00:00:00

abstract：:Terpenes are specialized plant metabolites that act as attractants to pollinators and as defensive compounds against pathogens and herbivores, but they also play an important role in determining the quality of horticultural food products. We show that the genome of cultivated apple (Malus domestica) contains 55 putati...

journal_title：Plant physiology

authors： Nieuwenhuizen NJ,Green SA,Chen X,Bailleul EJ,Matich AJ,Wang MY,Atkinson RG

更新日期：2013-02-01 00:00:00

abstract：:By comparing growth under five different temperature and irradiance regimes (20[deg]C and 800, 250, and 50[mu]mol m-2 s-1 and 5[deg]C and 250 and 50 [mu]mul m-2 s-1), we have examined the effects of light, temperature, and the relative reduction state of photosystem II on plant morphology, freezing tolerance (lethal t...

journal_title：Plant physiology

authors： Gray GR,Chauvin LP,Sarhan F,Huner N

更新日期：1997-06-01 00:00:00

abstract：:We investigated the role of gibberellins (GAs) in the effect of pat-2, a recessive mutation that induces facultative parthenocarpic fruit development in tomato (Lycopersicon esculentum Mill.) using near-isogenic lines with two different genetic backgrounds. Unpollinated wild-type Madrigal (MA/wt) and Cuarenteno (CU/wt...

journal_title：Plant physiology

authors： Fos M,Nuez F,García-Martínez JL

更新日期：2000-02-01 00:00:00

abstract：:The nitrite-reducing activity of the normal susceptible biotype of lambsquarters (Chenopodium album L.) was strongly inhibited by atrazine in the assay medium, both in the case of the in vivo assays of leaf discs in light, and in vitro photoreduction assays of crude extracts. In vitro assays of crude extracts with met...

journal_title：Plant physiology

authors： Lawrence JM,Foster RJ,Herrick HE

更新日期：1980-05-01 00:00:00

abstract：:This work was undertaken in an effort to reconcile the conflicting proline-accumulating responses of the barley (Hordeum vulgare L.) cultivars, Excelsior and Proctor, reported by Singh et al. (1972) and Hanson et al. (1976). It deals with the effects of different vapor pressure deficits (VPD) during growth and subsequ...

journal_title：Plant physiology

authors： Naidu BP,Aspinall D,Paleg LG

更新日期：1992-02-01 00:00:00

abstract：:In Chlamydomonas reinhardtii, a light-induced oxidative stress shifts the glutathione pool toward its oxidized form, resulting in a translational arrest of the large subunit (LSU) of Rubisco. We show here that the translational arrest of LSU is tightly coordinated with cessation of Rubisco assembly, and both processes...

journal_title：Plant physiology

authors： Cohen I,Knopf JA,Irihimovitch V,Shapira M

更新日期：2005-02-01 00:00:00

abstract：:A method has been devised for loading chloroplast inner envelope vesicles prepared from pea (Pisum sativum L. var Progress No. 9) or spinach (Spinacia oleracea L.) with 8-hydroxypyrene-1,3,6-trisulfonate (pyranine), a membrane impermeant, fluorescent pH indicator. Two known proton-linked transport activities of the in...

journal_title：Plant physiology

authors： Howitz KT,McCarty RE

更新日期：1988-04-01 00:00:00

abstract：:Abscisic acid (ABA) in the stem of akamatsu (Pinus densiflora) was identified and quantified by gas chromatography-mass spectrometry-selected ion monitoring using hexadeuterated ABA as an internal standard. tert-Butyldimethylsilyl ester was used as a derivative of ABA. This derivative had high sensitivity and selectiv...

journal_title：Plant physiology

authors： Funada R,Sugiyama T,Kubo T,Fushitani M

更新日期：1988-11-01 00:00:00

abstract：:Polyadenylated RNA was isolated from maize leaves and translated in vitro. In agreement with a previous report by others, we found among the translation products a 110-kilodalton pyruvate orthophosphate dikinase (PPDK) precursor that is about 16 kilodaltons larger than the polypeptide isolated from cells. This maize P...

journal_title：Plant physiology

authors： Aoyagi K,Bassham JA

更新日期：1985-08-01 00:00:00

abstract：:A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result ...

journal_title：Plant physiology

authors： Bölling C,Fiehn O

更新日期：2005-12-01 00:00:00

abstract：:The first committed step in the conversion of cycloartenol into Delta(5) C24-alkyl sterols in plants is catalyzed by an S-adenosyl-methionine-dependent sterol-C24-methyltransferase type 1 (SMT1). We report the consequences of overexpressing SMT1 in tobacco (Nicotiana tabacum), under control of either the constitutive ...

journal_title：Plant physiology

authors： Holmberg N,Harker M,Gibbard CL,Wallace AD,Clayton JC,Rawlins S,Hellyer A,Safford R

更新日期：2002-09-01 00:00:00

abstract：:The metabolic intermediate acetyl-CoA links anabolic and catabolic processes and coordinates metabolism with cellular signaling by influencing protein acetylation. In this study we demonstrate that in Arabidopsis (Arabidopsis thaliana), two distinctly localized acetate-activating enzymes, ACETYL-COA SYNTHETASE (ACS) i...

journal_title：Plant physiology

authors： Fu X,Yang H,Pangestu F,Nikolau BJ

更新日期：2020-03-01 00:00:00

abstract：:Cell-cell and extracellular cell matrix (ECM) interactions provide cells with information essential for controlling morphogenesis, cell-fate specification, and cell death. In animals, one of the major groups of enzymes that degrade the ECM is the matrix metalloproteinases (MMPs). Here, we report the characterization o...

journal_title：Plant physiology

authors： Delorme VG,McCabe PF,Kim DJ,Leaver CJ

更新日期：2000-07-01 00:00:00

abstract：:Cysthathionine [gamma]-synthase (CS), an enzyme involved in methionine biosynthesis, was purified from an acetone powder prepared from wheat (Triticum aestivum L.). After several chromatographic steps and radiolabeling of the partially purified enzyme with sodium cyanoboro[3H]hydride, a single polypeptide with a molec...

journal_title：Plant physiology

authors： Kreft BD,Townsend A,Pohlenz HD,Laber B

更新日期：1994-04-01 00:00:00

abstract：:In vitro fatty acid transfer to form complex lipids was observed in crude cell extracts of Anabaena variabilis using [1-(14)C]palmitoyl-acyl carrier protein, [1-(14)C]stearoyl-acyl carrier protein, and [1-(14)C]oleoyl-acyl carrier protein substrates. The data indicated that there was a rapid transfer of the fatty acid...

journal_title：Plant physiology

authors： Lem NW,Stumpf PK

更新日期：1984-07-01 00:00:00

abstract：:In the absence of sulfur (S), Chlamydomonas reinhardtii increases the abundance of several transcripts encoding proteins associated with S acquisition and assimilation, conserves S amino acids, and acclimates to suboptimal growth conditions. A positive regulator, SAC1 (for sulfur acclimation protein 1), and a negative...

journal_title：Plant physiology

authors： Gonzalez-Ballester D,Pollock SV,Pootakham W,Grossman AR

更新日期：2008-05-01 00:00:00

abstract：:Inoculation of 2-day-old cowpea (Vigna unguiculata [L.] Walp.) seedlings with Rhizobium fredii USDA257 results in proficient nodulation of the tap root. The most abundant nodulation occurs in a region roughly corresponding to the position of the root tip at the time of inoculation. We have examined plant gene expressi...

journal_title：Plant physiology

authors： Trese AT,Pueppke SG

更新日期：1990-04-01 00:00:00

abstract：:Nitrate or ammonium was added to soybean (Glycine max L. Merrill cv Corsoy) plants grown in plastic pouches 10 days after nodules first appeared. By the third day of treatment with 10 millimolar nitrate, nitrogenase specific activity (per unit nodule weight) had decreased to 15% to 25% of that of untreated plants. Lon...

journal_title：Plant physiology

authors： Noel KD,Carneol M,Brill WJ

更新日期：1982-11-01 00:00:00

abstract：:Root respiration associated with symbiotic fixation in soybean (Glycine max [L.] Merr.) was estimated by four methods.Averaged over the life of the plant, the root respires 5.8 milligrams C per milligram N accumulated from fixation. When nitrogenase (C(2)H(2)) activity and root respiration were decreased by treating r...

journal_title：Plant physiology

authors： Patterson TG,Larue TA

更新日期：1983-07-01 00:00:00

abstract：:Activity of the enzyme ADPglucose pyrophosphorylase is known to be reduced in maize (Zea mays L.) endosperm mutants at two independent loci, Shrunken-2 (Sh(2)) and Brittle-2 (Bt(2)). Spinach leaf ADPglucose pyrophosphorylase has previously been shown to comprise two subunits of 51 and 54 kilodaltons. Anti-bodies raise...

journal_title：Plant physiology

authors： Preiss J,Danner S,Summers PS,Morell M,Barton CR,Yang L,Nieder M

更新日期：1990-04-01 00:00:00

abstract：:Oilseed plants like Arabidopsis (Arabidopsis thaliana) develop green photosynthetically active embryos. Upon seed maturation, the embryonic chloroplasts degenerate into a highly reduced plastid type called the eoplast. Upon germination, eoplasts redifferentiate into chloroplasts and other plastid types. Here, we descr...

journal_title：Plant physiology

authors： Ruppel NJ,Logsdon CA,Whippo CW,Inoue K,Hangarter RP

更新日期：2011-01-01 00:00:00

abstract：:The amino acid antimetabolite, DL-p-fluorophenylalanine (FPA), inhibited induction of flowering in the short-day cocklebur plant, Xanthium pensylvanicum Wall., primarily by interfering with processes occurring during the inductive dark period. At the concentrations used the inhibitor had little effect on subsequent ve...

journal_title：Plant physiology

authors： Miller J,Ross C

更新日期：1966-09-01 00:00:00