Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivation.

Abstract:

:A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of which more than 100 could be identified. Analyte responses could be determined mostly with ses less than 10%. Wild-type cells of C. reinhardtii strain CC-125 subjected to nitrogen-, phosphorus-, sulfur-, or iron-depleted growth conditions develop highly distinctive metabolite profiles. Individual metabolites undergo marked changes in their steady-state levels. Compared to control conditions, sulfur-depleted cells accumulated 4-hydroxyproline more than 50-fold, whereas the amount of 2-ketovaline was reduced to 2% of control levels. The contribution of each compound to the differences observed in the metabolic phenotypes is summarized in a quantitatively rigorous way by principal component analysis, which clearly discriminates the cells from different growth regimes and indicates that phosphorus-depleted conditions induce a deficiency syndrome quite different from the response to nitrogen, sulfur, or iron starvation.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Bölling C,Fiehn O

doi

10.1104/pp.105.071589

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

1995-2005

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.105.071589

journal_volume

139

pub_type

杂志文章
  • Dynamic Maize Responses to Aphid Feeding Are Revealed by a Time Series of Transcriptomic and Metabolomic Assays.

    abstract::As a response to insect attack, maize (Zea mays) has inducible defenses that involve large changes in gene expression and metabolism. Piercing/sucking insects such as corn leaf aphid (Rhopalosiphum maidis) cause direct damage by acquiring phloem nutrients as well as indirect damage through the transmission of plant vi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01039

    authors: Tzin V,Fernandez-Pozo N,Richter A,Schmelz EA,Schoettner M,Schäfer M,Ahern KR,Meihls LN,Kaur H,Huffaker A,Mori N,Degenhardt J,Mueller LA,Jander G

    更新日期:2015-11-01 00:00:00

  • Calcium-regulated proteolysis of eEF1A.

    abstract::Eukaryotic elongation factor 1alpha (eEF1A) can be post-translationally modified by the addition of phosphorylglycerylethanolamine (PGE). [(14)C]Ethanolamine was incorporated into the PGE modification, and with carrot (Daucus carota L.) suspension culture cells, eEF1A was the only protein that incorporated detectable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.957

    authors: Ransom-Hodgkins WD,Brglez I,Wang X,Boss WF

    更新日期:2000-03-01 00:00:00

  • The biogenesis of ethylene in penicillium digitatum.

    abstract::The origin of the ethylene carbon skeleton in Penicillium digitatum appears to be intimately associated with the Krebs cycle acids, particularly the middle carbon atoms of dicarboxylic acids. Among the other compounds studied, certain carbon atoms of beta-alanine, propionic acid, and methionine can be incorporated int...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.12.1959

    authors: Jacobsen DW,Wang CH

    更新日期:1968-12-01 00:00:00

  • Auxin-binding Sites of Maize Coleoptiles Are Localized on Membranes of the Endoplasmic Reticulum.

    abstract::Sites in maize (Zea mays L.) coleoptile homogenates that reversibly bind naphthalene-1-acetic acid with high affinity and may represent receptor sites for auxins are located primarily on cellular membranes that show the enzymic and buoyant density characteristics of membranes of the rough endoplasmic reticulum. The si...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.4.594

    authors: Ray PM

    更新日期:1977-04-01 00:00:00

  • Expression of Umbelopsis ramanniana DGAT2A in seed increases oil in soybean.

    abstract::Oilseeds are the main source of lipids used in both food and biofuels. The growing demand for vegetable oil has focused research toward increasing the amount of this valuable component in oilseed crops. Globally, soybean (Glycine max) is one of the most important oilseed crops grown, contributing about 30% of the vege...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.123042

    authors: Lardizabal K,Effertz R,Levering C,Mai J,Pedroso MC,Jury T,Aasen E,Gruys K,Bennett K

    更新日期:2008-09-01 00:00:00

  • A white spruce gene catalog for conifer genome analyses.

    abstract::Several angiosperm plant genomes, including Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), poplar (Populus trichocarpa), and grapevine (Vitis vinifera), have been sequenced, but the lack of reference genomes in gymnosperm phyla reduces our understanding of plant evolution and restricts the potential impacts ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.179663

    authors: Rigault P,Boyle B,Lepage P,Cooke JE,Bousquet J,MacKay JJ

    更新日期:2011-09-01 00:00:00

  • The role of cytokinin in sieve tube regeneration and callose production in wounded coleus internodes.

    abstract::Cytokinin proved to be a controlling factor in sieve tube regeneration around wounded collateral bundles in an in vivo system in which the endogenous cytokinin level had been minimized. Both kinetin and zeatin were applied in aqueous solution to the bases of excised, mature internodes of Coleus blumei Benth. that had ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.3.982

    authors: Aloni R,Baum SF,Peterson CA

    更新日期:1990-07-01 00:00:00

  • BIN2, a new brassinosteroid-insensitive locus in Arabidopsis.

    abstract::Brassinosteroids (BRs) play important roles throughout plant development. Although many genes have been identified that are involved in BR biosynthesis, genetic approaches in Arabidopsis have led to the identification of only one gene, BRI1, that encodes a membrane receptor for BRs. To expand our knowledge of the mole...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.127.1.14

    authors: Li J,Nam KH,Vafeados D,Chory J

    更新日期:2001-09-01 00:00:00

  • R2R3-NaMYB8 regulates the accumulation of phenylpropanoid-polyamine conjugates, which are essential for local and systemic defense against insect herbivores in Nicotiana attenuata.

    abstract::Although phenylpropanoid-polyamine conjugates (PPCs) occur ubiquitously in plants, their biological roles remain largely unexplored. The two major PPCs of Nicotiana attenuata plants, caffeoylputrescine (CP) and dicaffeoylspermidine, increase dramatically in local and systemic tissues after herbivore attack and simulat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.151738

    authors: Kaur H,Heinzel N,Schöttner M,Baldwin IT,Gális I

    更新日期:2010-03-01 00:00:00

  • Effect of cold acclimation on bulk tissue electrical impedance: I. Measurements with birdsfoot trefoil at subfreezing temperatures.

    abstract::The resistive and reactive components of electrical impedance were measured for birdsfoot trefoil (Lotus corniculatus L.) stems at freezing temperatures to -8 degrees C. As temperature decreased the specific resistance at frequencies between 49 hertz and 1.11 megahertz of stems from cold acclimated plants increased mo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.275

    authors: Stout DG

    更新日期:1988-01-01 00:00:00

  • StGCPRP, a potato gene strongly expressed in stomatal guard cells, defines a novel type of repetitive proline-rich proteins.

    abstract::Guard cells represent a highly differentiated cell type within the epidermis of plant leaves and stems. They respond to many endogenous and environmental signals and thereby modify the size of the stomatal pore they surround. We identified a novel gene that is highly expressed in guard cells of potato (Solanum tuberos...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.677

    authors: Menke U,Renault N,Mueller-Roeber B

    更新日期:2000-03-01 00:00:00

  • The Polyadenylation Factor Subunit CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30: A Key Factor of Programmed Cell Death and a Regulator of Immunity in Arabidopsis.

    abstract::Programmed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. Indeed, incompatible plant-pathogen interactions are well known to induce the hypersensitive response, a localized cell death. Mutational analyses have identified several key PCD components, and we r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.236083

    authors: Bruggeman Q,Garmier M,de Bont L,Soubigou-Taconnat L,Mazubert C,Benhamed M,Raynaud C,Bergounioux C,Delarue M

    更新日期:2014-06-01 00:00:00

  • Changes in the Content of Two Glutamate Synthase Proteins in Spikelets of Rice (Oryza sativa) Plants during Ripening.

    abstract::Nitrogen accumulation in the apical spikelets on the primary branches of the main stem of rice plants have been studied during the ripening process (0-35 d after flowering). The level of NADH-dependent glutamate synthase (GOGAT) protein and activity increased 4- and 6-fold, respectively, in the first 15 d after flower...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.4.1257

    authors: Hayakawa T,Yamaya T,Mae T,Ojima K

    更新日期:1993-04-01 00:00:00

  • Oxygen Uptake and Photosynthesis of the Red Macroalga, Chondrus crispus, in Seawater: Effects of Oxygen Concentration.

    abstract::With an experimental system developed for aquatic plants using the mass spectrometry technique and infrared gas analysis of CO(2), we studied the responses to various O(2) concentrations of gas exchanges with the red macroalga Chondrus crispus S. The results were as follows. (a) Irrespective of the CO(2) concentration...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.3.545

    authors: Brechignac F,Andre M

    更新日期:1985-07-01 00:00:00

  • Metabolism of tween-Fatty Acid esters by cultured soybean cells : kinetics of incorporation into lipids, subsequent turnover, and associated changes in endogenous Fatty Acid synthesis.

    abstract::Uptake of Tween-fatty acid esters and incorporation of the fatty acids into lipids by soybean (Glycine max [L.] Merr.) suspension cultures was investigated, together with subsequent turnover of the incorporated fatty acids and associated changes in endogenous fatty acid synthesis. Tween uptake was saturable, and fatty...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.3.780

    authors: Terzaghi WB

    更新日期:1986-11-01 00:00:00

  • Isolation and Freezing Tolerances of Mesophyll Cells from Cold-hardened and Nonhardened Winter Rye.

    abstract::A method is described for the release of large quantities of mesophyll cells from leaves of both cold-hardened and nonhardened winter rye seedlings by a combination of enzymic and physical maceration. Such preparations usually contain a certain percentage of nonviable cells and are thus not suitable for quantitative b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.5.906

    authors: Singh J

    更新日期:1981-05-01 00:00:00

  • Rapid osmotic adjustment by a succulent halophyte to saline shock.

    abstract::The objective of this research was to measure the short term osmotic adjustment of Salicornia europaea L. ssp. rubra (A. Nels) Breitung when suddenly exposed to 100 millimolar NaCl. Plants were grown hydroponically, shocked with 100 millimolar NaCl added to the culture solution, and stem tips analyzed for free inorgan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.1.100

    authors: McNulty IB

    更新日期:1985-05-01 00:00:00

  • Active Transport of Inorganic Carbon Increases the Rate of O(2) Photoreduction by the Cyanobacterium Synechococcus UTEX 625.

    abstract::Chlorophyll a fluorescence of Synechococcus UTEX 625 was quenched during the transport of inorganic carbon, even when CO(2) fixation was inhibited by iodoacetamide. Measurements with a pulse modulation fluorometer showed that at least 75% of the quenching was due to oxidation of Q(a), the primary acceptor of photosyst...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.6

    authors: Miller AG,Espie GS,Canvin DT

    更新日期:1988-09-01 00:00:00

  • Giant flowers of Southern magnolia are hydrated by the xylem.

    abstract::Flowering depends upon long-distance transport to supply water for reproductive mechanisms to function. Previous physiological studies suggested that flowers operated uncoupled from stem xylem transport and received water primarily from the phloem. We demonstrate that the water balance of Southern magnolia (Magnolia g...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.136127

    authors: Feild TS,Chatelet DS,Brodribb TJ

    更新日期:2009-07-01 00:00:00

  • Genome-wide detection of condition-sensitive alternative splicing in Arabidopsis roots.

    abstract::Iron (Fe) deficiency is a world-wide nutritional disorder in both plants and humans, resulting from its restricted bioavailability for plants and, subsequently, low Fe concentration in edible plant parts. Plants have evolved sophisticated mechanisms to alleviate Fe deficiency, with the aim of recalibrating metabolic f...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.217778

    authors: Li W,Lin WD,Ray P,Lan P,Schmidt W

    更新日期:2013-07-01 00:00:00

  • C4 Photosynthetic Gene Expression in Light- and Dark-Grown Amaranth Cotyledons.

    abstract::The patterns of expression for genes encoding several C4 photosynthetic enzymes were examined in light-grown and dark-grown (etiolated) cotyledons of amaranth (Amaranthus hypochondriacus), a dicotyledonous C4 plant. The large subunit and small subunit of ribulose-1,5-bisphosphate carboxylase (RuBPCase), phosphoenolpyr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.4.1085

    authors: Wang JL,Long JJ,Hotchkiss T,Berry JO

    更新日期:1993-08-01 00:00:00

  • Sulfur transfer through an arbuscular mycorrhiza.

    abstract::Despite the importance of sulfur (S) for plant nutrition, the role of the arbuscular mycorrhizal (AM) symbiosis in S uptake has received little attention. To address this issue, 35S-labeling experiments were performed on mycorrhizas of transformed carrot (Daucus carota) roots and Glomus intraradices grown monoxenicall...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.129866

    authors: Allen JW,Shachar-Hill Y

    更新日期:2009-01-01 00:00:00

  • Association between Membrane Phase Properties and Dehydration Injury in Soybean Axes.

    abstract::Axes of soybean seeds are tolerant to dehydration at 6 hours of imbibition, but susceptible to dehydration injury if dried at 36 hours of imbibition. Smooth microsomal membranes were isolated from axes imbibed for 6 hours (dehydration tolerant state) and 36 hours (dehydration susceptible state) before and after dehydr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.3.759

    authors: Senaratna T,McKersie BD,Stinson RH

    更新日期:1984-11-01 00:00:00

  • Tracing cationic nutrients from xylem into stem tissue of French bean by stable isotope tracers and cryo-secondary ion mass spectrometry.

    abstract::Fluxes of mineral nutrients in the xylem are strongly influenced by interactions with the surrounding stem tissues and are probably regulated by them. Toward a mechanistic understanding of these interactions, we applied stable isotope tracers of magnesium, potassium, and calcium continuously to the transpiration strea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.143776

    authors: Metzner R,Schneider HU,Breuer U,Thorpe MR,Schurr U,Schroeder WH

    更新日期:2010-02-01 00:00:00

  • Calcium in the regulation of gravitropism by light.

    abstract::The red light requirement for positive gravitropism in roots of corn (Zea mays cv "Merit") provides an entry for examining the participation of calcium in gravitropism. Applications of calcium chelators inhibit the light response. Calcium channel blockers (verapamil, lanthanum) can also inhibit the light response, a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.4.1276

    authors: Perdue DO,LaFavre AK,Leopold AC

    更新日期:1988-01-01 00:00:00

  • Citrate-permeable channels in the plasma membrane of cluster roots from white lupin.

    abstract::White lupin (Lupinus albus) is well adapted to phosphorus deficiency by developing cluster roots that release large amounts of citrate into the rhizosphere to mobilize the sparingly soluble phosphorus. To determine the mechanism underlying citrate release from cluster roots, we isolated protoplasts from different type...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.046201

    authors: Zhang WH,Ryan PR,Tyerman SD

    更新日期:2004-11-01 00:00:00

  • Effect of Night Temperature on the Activity of Sucrose Phosphate Synthase, Acid Invertase, and Sucrose Synthase in Source and Sink Tissues of Rosa hybrida cv Golden Times.

    abstract::Sucrose phosphate synthase and acid invertase activities in the mature leaves of roses (Rosa hybrida cv Golden Times) were greater in plants grown under a higher night temperature than under a lower temperature regime. In young shoots, the activity of acid invertase was promoted by the lower temperature while that of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.2.447

    authors: Khayat E,Zieslin N

    更新日期:1987-06-01 00:00:00

  • Predicting Stomatal Closure and Turgor Loss in Woody Plants Using Predawn and Midday Water Potential.

    abstract::Knowledge about physiological stress thresholds provides crucial information about plant performance and survival under drought. In this study, we report on the triphasic nature of the relationship between plant water potential (Ψ) at predawn and midday and describe a method that predicts Ψ at stomatal closure and tur...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00500

    authors: Knipfer T,Bambach N,Hernandez MI,Bartlett MK,Sinclair G,Duong F,Kluepfel DA,McElrone AJ

    更新日期:2020-10-01 00:00:00

  • Proper levels of the Arabidopsis cohesion establishment factor CTF7 are essential for embryo and megagametophyte, but not endosperm, development.

    abstract::CTF7 is an essential gene in yeast that is required for the formation of sister chromatid cohesion. While recent studies have provided insights into how sister chromatid cohesion is established, less is known about how specifically CTF7 facilitates the formation of cohesion, and essentially nothing is known about how ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.157560

    authors: Jiang L,Yuan L,Xia M,Makaroff CA

    更新日期:2010-10-01 00:00:00

  • Arabidopsis putative selenium-binding protein1 expression is tightly linked to cellular sulfur demand and can reduce sensitivity to stresses requiring glutathione for tolerance.

    abstract::Selenium-Binding Protein1 (SBP1) gene expression was studied in Arabidopsis (Arabidopsis thaliana) seedlings challenged with several stresses, including cadmium (Cd), selenium {selenate [Se(VI)] and selenite [Se(IV)]}, copper (Cu), zinc (Zn), and hydrogen peroxide (H(2)O(2)) using transgenic lines expressing the lucif...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.144808

    authors: Hugouvieux V,Dutilleul C,Jourdain A,Reynaud F,Lopez V,Bourguignon J

    更新日期:2009-10-01 00:00:00