StGCPRP, a potato gene strongly expressed in stomatal guard cells, defines a novel type of repetitive proline-rich proteins.

Abstract:

:Guard cells represent a highly differentiated cell type within the epidermis of plant leaves and stems. They respond to many endogenous and environmental signals and thereby modify the size of the stomatal pore they surround. We identified a novel gene that is highly expressed in guard cells of potato (Solanum tuberosum). It encodes a repetitive proline (Pro)-rich protein of 54 kD (491 amino acids) and was named StGCPRP (S. tuberosum guard cell Pro-rich protein). StGCPRP has a bipartite structure. The C-terminal part of StGCPRP contains a high percentage (46%) of Pro residues organized in distinct repetitive sequence motifs, whereas its extended N terminus is essentially free of Pros. StGCPRP represents the first member of a novel class of hybrid Pro-rich proteins that we designated NHyPRPs. In young but not in mature leaves, StGCPRP transcripts were also present at high levels in mesophyll cells (in addition to guard cells), indicating developmental regulation of StGCPRP gene expression. In addition, StGCPRP expression is regulated by environmental factors, as shown by a decrease in StGCPRP transcript levels under drought stress. Two proteins similar to StGCPRP were found to be encoded by the Arabidopsis genome, indicating that NHyPRPs are more widely distributed in higher plants.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Menke U,Renault N,Mueller-Roeber B

doi

10.1104/pp.122.3.677

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

677-86

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

122

pub_type

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