Abstract:
:Chlamydomonas reinhardtii adapts to the stress of CO(2)-limiting conditions through the induction of a set of genes including CAH1, which encodes a periplasmic carbonic anhydrase. CAH1 is up-regulated under low-CO(2) conditions (air containing 0.04% [v/v] CO(2)) in the presence of light, whereas it is down-regulated under high-CO(2) conditions (5% [v/v] CO(2)) or in the dark. In an effort to identify cis-elements involved in the transcriptional regulation of CAH1, a series of 5'-nested deletions of the region upstream of CAH1 were fused to a promoterless arylsulfatase reporter gene (ARS). The upstream region from -651 to +41 relative to the transcription start site was sufficient to regulate the expression of ARS with kinetics similar to those of endogenous CAH1. Deletion of the region between -651 and -294 resulted in a significant decrease in the level of arylsulfatase activity expressed under low-CO(2) conditions. The 543-bp upstream region from -651 to -109, without any promoter elements, CAAT-box, or TATA-box, could confer CO(2) and light responsiveness on the beta(2)-tubulin minimal promoter. This 543-bp region was divided into two parts: a 358-bp silencer region from -651 to -294, which represses the minimal promoter activity under high-CO(2) conditions, and a 185-bp enhancer region from -293 to -109, which activates the promoter under low-CO(2) conditions in the presence of light.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kucho Ki,Ohyama K,Fukuzawa Hdoi
10.1104/pp.121.4.1329subject
Has Abstractpub_date
1999-12-01 00:00:00pages
1329-38issue
4eissn
0032-0889issn
1532-2548journal_volume
121pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Acropetal mass flow of water is demonstrated in two submerged angiosperms, Lobelia dortmanna L. and Sparganium emersum Rehman by means of guttation measurements. Transpiration is absent in truly submerged plants, but the presence of guttation verifies that long-distance water transport takes place. Use of tritiated wa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.4.1369
更新日期:1993-12-01 00:00:00
abstract::Calcium phosphate-boric acid treatments and UDP-glucose both elicited aniline blue fluorescent, periodic acid-Schiff's reagent-resistant, deposits in association with the cell walls of cowpea (Vigna sinensis [Torner] Savi cv. Early Ramshorn) tissue. Those deposits induced by calcium phosphateboric acid treatment ultra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.2.366
更新日期:1982-02-01 00:00:00
abstract::The effect of light intensity on the rates of ammonium and nitrate uptake and of CO(2) fixation has been determined in intact Anacystis nidulans cells. Ammonium uptake became saturated at photon flux values of about 60 microeinsteins per square meter per second, whereas both nitrate uptake and CO(2) fixation reached s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.686
更新日期:1986-06-01 00:00:00
abstract::Endogenous factors which determine the photosynthetic capacity of the leaf were studied in the fully expanded, primary leaves of young seedings of bean (cv. Bulgarian). Following removal of the shoot above the primary leaf node and excision of all axillary buds, the primary leaves increased in area and thickness, in c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.2.285
更新日期:1979-08-01 00:00:00
abstract::The relationship between the rate of orthophosphate (Pi) transport into the stroma and the rate of CO(2) fixation by intact chloroplasts was investigated. High Pi concentrations in the medium lead to a depletion of stromal metabolites, due to excessive Pi transport into the stroma, resulting in the inhibition of CO(2)...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.4.574
更新日期:1980-04-01 00:00:00
abstract::The esterification of methylecgonine (2-carbomethoxy-3β-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase. The enzyme is capabl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.248187
更新日期:2015-01-01 00:00:00
abstract::This study examines the capacity of intact spinach (Spinacia oleracea L.) chloroplasts to fix (14)CO(2) when supplied with Benson-Calvin cycle intermediates in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). Under these conditions, substantial (14)CO(2) fixation occurred in the light but not in the dar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.2.321
更新日期:1983-06-01 00:00:00
abstract::The ATPase activity of a membrane fraction from soybean (Glycine max L.) root and callus cells, presumed to be enriched in plasma membrane, has been characterized with respect to ion stimulation, pH requirement, and nucleotide specificity. The enzyme from both sources was activated by divalent cations (Mg(2+) > Mn(2+)...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.2.264
更新日期:1977-02-01 00:00:00
abstract::We used pulsed-field gel electrophoresis and restriction fragment mapping to analyze the structure of Medicago truncatula chloroplast DNA (cpDNA). We find most cpDNA in genome-sized linear molecules, head-to-tail genomic concatemers, and complex branched forms with ends at defined sites rather than at random sites as ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.112946
更新日期:2008-03-01 00:00:00
abstract::A circadian rhythm of O(2) evolution has been found in Euglena gracilis, Klebs strain Z. The rhythm persists for at least 5 days in constant dim light and temperature, but damps out in constant bright light. The phase of this rhythm can be shifted by a pulse of bright light and the period length is not changed over a ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.2.150
更新日期:1978-02-01 00:00:00
abstract::The trans-Golgi network (TGN) plays a central role in cellular secretion and has been implicated in sorting cargo destined for the plasma membrane. Previously, the Arabidopsis (Arabidopsis thaliana) echidna (ech) mutant was shown to exhibit a dwarf phenotype due to impaired cell expansion. However, ech also has a prev...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.227769
更新日期:2014-03-01 00:00:00
abstract::Indoleacetic acid oxidase in tobacco callus tissues (Nicotiana tabacum L., cultivar White Gold) was resolved into seven anionic isoenzymes by polyacrylamide gel disc electrophoresis. Different concentrations of kinetin and zeatin in the presence of indoleacetic acid affected the level of this enzyme, particularly two ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.2.181
更新日期:1971-02-01 00:00:00
abstract::In earlier ecophysiological studies that were conducted on Arabidopsis halleri plants, scientists focused on the mechanisms of Cd and Zn hyperaccumulation but did not take into consideration the environmental factors that can significantly affect the physiological responses of plants in situ. In this study, we investi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00212
更新日期:2017-09-01 00:00:00
abstract::Maize plants (Zea mays) attacked by caterpillars release a mixture of odorous compounds that attract parasitic wasps, natural enemies of the herbivores. We assessed the genetic variability of these induced volatile emissions among 31 maize inbred lines representing a broad range of genetic diversity used by breeders i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.039891
更新日期:2004-08-01 00:00:00
abstract::Stimulation of active oxygen metabolism occurs during the early stages of interactions involving bacteria and plant cell suspensions. Although many cellular processes are known to affect active oxygen metabolism in plants, it is not known which of these factors affect active oxygen levels during plant-bacteria interac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.353
更新日期:1995-05-01 00:00:00
abstract::Novel cyanogenic plants have been generated by the simultaneous expression of the two multifunctional sorghum (Sorghum bicolor [L.] Moench) cytochrome P450 enzymes CYP79A1 and CYP71E1 in tobacco (Nicotiana tabacum cv Xanthi) and Arabidopsis under the regulation of the constitutive 35S promoter. CYP79A1 and CYP71E1 cat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.4.1437
更新日期:2000-08-01 00:00:00
abstract::Photosynthetic rate, ribulose 1,5-bisphosphate carboxylase activity, specific leaf weight, and leaf concentrations of carbohydrates, proteins, chlorophyll, and inorganic phosphate were determined periodically from midbloom until maturity in leaves of soybean plants (Glycine max L., var. Hodgson) from which reproductiv...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.3.394
更新日期:1978-03-01 00:00:00
abstract::We examined the influence of aluminum and calcium (and certain other cations) on hormone transport in corn roots. When aluminum was applied unilaterally to the caps of 15 mm apical root sections the roots curved strongly away from the aluminum. When aluminum was applied unilaterally to the cap and 3H-indole-3-acetic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.3.890
更新日期:1988-01-01 00:00:00
abstract::Pear fruit cells (Pyrus communis L. cv Passe Crassane) stopped dividing when subcultured in a bioreactor under auxin starvation in the presence of 0.37 molar mannitol. The cessation of cell division was preceded by the accumulation of a specific basic polypeptide of 24 kilodalton. Readdition of 2.3 micromolar 2,4-dich...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.2.400
更新日期:1987-10-01 00:00:00
abstract::Recently, a feedback inhibition of the chloroplastic 1-deoxy-D-xylulose 5-phosphate (DXP)/2-C-methyl-D-erythritol 4-phosphate (MEP) pathway of isoprenoid synthesis by end products dimethylallyl diphosphate (DMADP) and isopentenyl diphosphate (IDP) was postulated, but the extent to which DMADP and IDP can build up is n...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00470
更新日期:2015-06-01 00:00:00
abstract::The plant growth retardant alpha-cyclopropyl-alpha-(4-methyoxyphenyl)-5-pyrimidine methyl alcohol (ancymidol) and a series of analogs of this substance in which one or more of the substituents were varied were tested for their comparative biological activity. The compounds were tested as inhibitors of internode elonga...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.3.707
更新日期:1982-03-01 00:00:00
abstract::The flavoprotein (R)-(+)-mandelonitrile lyase (MDL; EC 4.1.2.10) plays a key role in cyanogenesis in rosaceous stone fruits. An MDL gene (mdl3) and its corresponding cDNA (MDL3) were isolated from black cherry (Prunus serotina) and characterized. The mdl3 gene contains 2292 bp of the 5' flanking region, the entire cod...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.4.1359
更新日期:1997-12-01 00:00:00
abstract::Explants of genetic tumors, tumors initiated by Agrobacterium tumefaciens strains B-6 and T-37, and excised pith plugs from Nicotiana glauca, N. langsdorffii, and N. glauca-langsdorffii were cultured on Murashige and Skoog medium. All cultures, pith callus and tumors with the exception of N. langsdorffii pith grew on ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.7.1073
更新日期:1969-07-01 00:00:00
abstract::Despite much study of the role of S-adenosylmethionine (SAM) in the methylation of DNA, RNA, and proteins, and as a cofactor for a wide range of biosynthetic processes, little is known concerning the intracellular transport of this essential metabolite. Screening of the Arabidopsis (Arabidopsis thaliana) genome yielde...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086975
更新日期:2006-11-01 00:00:00
abstract::Four legume species (four genera) were examined and found to contain hemagglutinins with properties similar to those which we have previously described for the enzymic hemagglutinin in Vigna radiata. Examination of extracts by gel filtration and ion exchange chromatography showed that an alpha-galactosidase activity e...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.4.618
更新日期:1980-04-01 00:00:00
abstract::Carbonic anhydrase (CA) (EC 4.2.1.1) enzymes catalyze the reversible hydration of CO2, a reaction that is important in many physiological processes. We have cloned and sequenced a full-length cDNA encoding an intracellular beta-CA from the unicellular green alga Coccomyxa. Nucleotide sequence data show that the isolat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.4.1341
更新日期:1998-08-01 00:00:00
abstract::Histone H2B monoubiquitination (H2Bub1) is an important regulatory mechanism in eukaryotic gene transcription and is essential for normal plant development. However, the function of H2Bub1 in reproductive development remains elusive. Here, we report rice (Oryza sativa) HISTONE MONOUBIQUITINATION1 (OsHUB1) and OsHUB2, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.256578
更新日期:2015-08-01 00:00:00
abstract::The mechanism of sucrose transport into vacuoles isolated from leaf tissue has been studied only in barley (Hordeum vulgare) mesophyll cells. In this tissue, sucrose transport was reported to be a facilitated diffusion. We have observed a facilitated diffusion of sucrose into vacuoles isolated from this tissue. Howeve...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.2.251
更新日期:1988-10-01 00:00:00
abstract::An apparatus for measuring simultaneously the surface electrical potentials along epicotyl was developed to study the largely bending situation by gravity. Potentials increased on the upper side and decreased on the lower side after the horizontal placement. The time course of electrical changes consisted of two compo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.1.193
更新日期:1991-09-01 00:00:00
abstract::The amino acid antimetabolite, DL-p-fluorophenylalanine (FPA), inhibited induction of flowering in the short-day cocklebur plant, Xanthium pensylvanicum Wall., primarily by interfering with processes occurring during the inductive dark period. At the concentrations used the inhibitor had little effect on subsequent ve...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.7.1185
更新日期:1966-09-01 00:00:00