Transgenic tobacco and Arabidopsis plants expressing the two multifunctional sorghum cytochrome P450 enzymes, CYP79A1 and CYP71E1, are cyanogenic and accumulate metabolites derived from intermediates in Dhurrin biosynthesis.

Abstract:

:Novel cyanogenic plants have been generated by the simultaneous expression of the two multifunctional sorghum (Sorghum bicolor [L.] Moench) cytochrome P450 enzymes CYP79A1 and CYP71E1 in tobacco (Nicotiana tabacum cv Xanthi) and Arabidopsis under the regulation of the constitutive 35S promoter. CYP79A1 and CYP71E1 catalyze the conversion of the parent amino acid tyrosine to p-hydroxymandelonitrile, the aglycone of the cyanogenic glucoside dhurrin. CYP79A1 catalyzes the conversion of tyrosine to p-hydroxyphenylacetaldoxime and CYP71E1, the subsequent conversion to p-hydroxymandelonitrile. p-Hydroxymandelonitrile is labile and dissociates into p-hydroxybenzaldehyde and hydrogen cyanide, the same products released from dhurrin upon cell disruption as a result of pest or herbivore attack. In transgenic plants expressing CYP79A1 as well as CYP71E1, the activity of CYP79A1 is higher than that of CYP71E1, resulting in the accumulation of several p-hydroxyphenylacetaldoxime-derived products in the addition to those derived from p-hydroxymandelonitrile. Transgenic tobacco and Arabidopsis plants expressing only CYP79A1 accumulate the same p-hydroxyphenylacetaldoxime-derived products as transgenic plants expressing both sorghum cytochrome P450 enzymes. In addition, the transgenic CYP79A1 Arabidopsis plants accumulate large amounts of p-hydroxybenzylglucosinolate. In transgenic Arabidopsis expressing CYP71E1, this enzyme and the enzymes of the pre-existing glucosinolate pathway compete for the p-hydroxyphenylacetaldoxime as substrate, resulting in the formation of small amounts of p-hydroxybenzylglucosinolate. Cyanogenic glucosides are phytoanticipins, and the present study demonstrates the feasibility of expressing cyanogenic compounds in new plant species by gene transfer technology to improve pest and disease resistance.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Bak S,Olsen CE,Halkier BA,Møller BL

doi

10.1104/pp.123.4.1437

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

1437-48

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

123

pub_type

杂志文章
  • FLAVODIIRON2 and FLAVODIIRON4 proteins mediate an oxygen-dependent alternative electron flow in Synechocystis sp. PCC 6803 under CO2-limited conditions.

    abstract::This study aims to elucidate the molecular mechanism of an alternative electron flow (AEF) functioning under suppressed (CO2-limited) photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], reaches a maximum shortly...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.249987

    authors: Shimakawa G,Shaku K,Nishi A,Hayashi R,Yamamoto H,Sakamoto K,Makino A,Miyake C

    更新日期:2015-02-01 00:00:00

  • Iron uptake by plants from microbial siderophores : a study with 7-nitrobenz-2 oxa-1,3-diazole-desferrioxamine as fluorescent ferrioxamine B analog.

    abstract::The synthetically produced fluorescent siderophore NBD-desferrioxamine B (NBD-DFO), an analog of the natural siderophore ferrioxamine B, was used to study iron uptake by plants. Short-term (10-hour) (55)Fe uptake rates by cotton (Gossypium spp.) and maize (Zea mays L.) plants from the modified siderophore were similar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.4.1329

    authors: Bar-Ness E,Hadar Y,Chen Y,Shanzer A,Libman J

    更新日期:1992-08-01 00:00:00

  • Synthesis and apparent turnover of Acid invertase in relation to invertase inhibitor in wounded sweet potato root tissue.

    abstract::Previously we showed that acid invertase activity increased and then decreased rapidly in wounded sweet potato (Ipomoea batatas Liam.) root tissue, and that the tissue contained a heat-stable, proteinaceous inhibitor with a molecular weight of about 19,500 daltons.In response to wounding of sweet potato root tissue, i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.5.879

    authors: Matsushita K,Uritani I

    更新日期:1977-05-01 00:00:00

  • Inhibition of Barnyardgrass 4-Hydroxyphenylpyruvate Dioxygenase by Sulcotrione.

    abstract::4-Hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) was partially purified from barnyardgrass (Echinochloa crus-galli L.) leaves and assayed by high-performance liquid chromatography analysis of product formation or by the capture of released 14CO2. The bleaching herbicide sulcotrione [2-(2-chloro-4-methanesulfonylben...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.4.1429

    authors: Secor J

    更新日期:1994-12-01 00:00:00

  • Role and interrelationship of Gα protein, hydrogen peroxide, and nitric oxide in ultraviolet B-induced stomatal closure in Arabidopsis leaves.

    abstract::Heterotrimeric G proteins have been shown to transmit ultraviolet B (UV-B) signals in mammalian cells, but whether they also transmit UV-B signals in plant cells is not clear. In this paper, we report that 0.5 W m(-2) UV-B induces stomatal closure in Arabidopsis (Arabidopsis thaliana) by eliciting a cascade of intrace...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.211623

    authors: He JM,Ma XG,Zhang Y,Sun TF,Xu FF,Chen YP,Liu X,Yue M

    更新日期:2013-03-01 00:00:00

  • It's complicated: intraroot system variability of respiration and morphological traits in four deciduous tree species.

    abstract::Within branched root systems, a distinct heterogeneity of traits exists. Knowledge about the ecophysiology of different root types is critical to understand root system functioning. Classification schemes have to match functional root types as closely as possible to be used for sampling and modeling. Among ecophysiolo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.240267

    authors: Rewald B,Rechenmacher A,Godbold DL

    更新日期:2014-10-01 00:00:00

  • Protein cryoprotective activity of a cytosolic small heat shock protein that accumulates constitutively in chestnut stems and is up-regulated by low and high temperatures.

    abstract::Heat shock, and other stresses that cause protein misfolding and aggregation, trigger the accumulation of heat shock proteins (HSPs) in virtually all organisms. Among the HSPs of higher plants, those belonging to the small HSP (sHSP) family remain the least characterized in functional terms. We analyzed the occurrence...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.035857

    authors: Lopez-Matas MA,Nuñez P,Soto A,Allona I,Casado R,Collada C,Guevara MA,Aragoncillo C,Gomez L

    更新日期:2004-04-01 00:00:00

  • Lignification in sugarcane: biochemical characterization, gene discovery, and expression analysis in two genotypes contrasting for lignin content.

    abstract::Sugarcane (Saccharum spp.) is currently one of the most efficient crops in the production of first-generation biofuels. However, the bagasse represents an additional abundant lignocellulosic resource that has the potential to increase the ethanol production per plant. To achieve a more efficient conversion of bagasse ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.225250

    authors: Bottcher A,Cesarino I,Santos AB,Vicentini R,Mayer JL,Vanholme R,Morreel K,Goeminne G,Moura JC,Nobile PM,Carmello-Guerreiro SM,Anjos IA,Creste S,Boerjan W,Landell MG,Mazzafera P

    更新日期:2013-12-01 00:00:00

  • Storage Protein Synthesis during Oat (Avena sativa L.) Seed Development.

    abstract::Oat (Avena sativa L.) seeds harvested at 2-day intervals from anthesis to maturity were tested for their ability to incorporate [(35)S]sulfate into protein. Incorporation of [(35)S]sulfate into TCA-insoluble material began 2 to 4 days postanthesis (DPA), reached a peak 14 to 16 DPA, and was barely detectable by 24 DPA...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.2.337

    authors: Luthe DS

    更新日期:1987-06-01 00:00:00

  • CO(2) Enhancement of Growth and Photosynthesis in Rice (Oryza sativa) : Modification by Increased Ultraviolet-B Radiation.

    abstract::Two cultivars of rice (Oryza sativa L.) IR-36 and Fujiyama-5 were grown at ambient (360 microbars) and elevated CO(2) (660 microbars) from germination through reproduction in unshaded greenhouses at the Duke University Phytotron. Growth at elevated CO(2) resulted in significant decreases in nighttime respiration and i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.2.473

    authors: Ziska LH,Teramura AH

    更新日期:1992-06-01 00:00:00

  • Growth of transplastomic cells expressing D-amino acid oxidase in chloroplasts is tolerant to D-alanine and inhibited by D-valine.

    abstract::Dual-conditional positive/negative selection markers are versatile genetic tools for manipulating genomes. Plastid genomes are relatively small and conserved DNA molecules that can be manipulated precisely by homologous recombination. High-yield expression of recombinant products and maternal inheritance of plastid-en...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.204107

    authors: Gisby MF,Mudd EA,Day A

    更新日期:2012-12-01 00:00:00

  • Biosynthesis of the Dihydrochalcone Sweetener Trilobatin Requires Phloretin Glycosyltransferase2.

    abstract::Epidemics of obesity and type 2 diabetes drive strong consumer interest in plant-based low-calorie sweeteners. Trilobatin is a sweetener found at high concentrations in the leaves of a range of crabapple (Malus) species, but not in domesticated apple (Malus × domestica) leaves, which contain trilobatin's bitter positi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00807

    authors: Wang Y,Yauk YK,Zhao Q,Hamiaux C,Xiao Z,Gunaseelan K,Zhang L,Tomes S,López-Girona E,Cooney J,Li H,Chagné D,Ma F,Li P,Atkinson RG

    更新日期:2020-10-01 00:00:00

  • Sterol C-24 methyltransferase type 1 controls the flux of carbon into sterol biosynthesis in tobacco seed.

    abstract::The first committed step in the conversion of cycloartenol into Delta(5) C24-alkyl sterols in plants is catalyzed by an S-adenosyl-methionine-dependent sterol-C24-methyltransferase type 1 (SMT1). We report the consequences of overexpressing SMT1 in tobacco (Nicotiana tabacum), under control of either the constitutive ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.004226

    authors: Holmberg N,Harker M,Gibbard CL,Wallace AD,Clayton JC,Rawlins S,Hellyer A,Safford R

    更新日期:2002-09-01 00:00:00

  • Ice-cap. A high-throughput method for capturing plant tissue samples for genotype analysis.

    abstract::High-throughput genotype screening is rapidly becoming a standard research tool in the post-genomic era. A major bottleneck currently exists, however, that limits the utility of this approach in the plant sciences. The rate-limiting step in current high-throughput pipelines is that tissue samples from living plants mu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.040774

    authors: Krysan P

    更新日期:2004-07-01 00:00:00

  • Diverse Allyl Glucosinolate Catabolites Independently Influence Root Growth and Development.

    abstract::Glucosinolates (GSLs) are sulfur-containing defense metabolites produced in the Brassicales, including the model plant Arabidopsis (Arabidopsis thaliana). Previous work suggests that specific GSLs may function as signals to provide direct feedback regulation within the plant to calibrate defense and growth. These GSLs...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00170

    authors: Katz E,Bagchi R,Jeschke V,Rasmussen ARM,Hopper A,Burow M,Estelle M,Kliebenstein DJ

    更新日期:2020-07-01 00:00:00

  • Localization, solubilization and characterization of plant membrane-associated calcium-dependent protein kinases.

    abstract::Membrane fractions from mature silver beet (Beta vulgaris) deveined leaf and leaf stem homogenates have associated Ca(2+) -dependent protein kinase. The Ca(2+) -dependent protein kinase activity is associated with plasma membranes (density 1.14-1.18 grams per cubic centimeter) as determined from copurification on isop...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.164

    authors: Klucis E,Polya GM

    更新日期:1988-09-01 00:00:00

  • Involvement of Macromolecule Biosynthesis in Auxin and Fusicoccin Enhancement of beta-Glucan Synthase Activity in Pea.

    abstract::In pea stem segments whose cuticle has been made permeable by abrading it, actinomycin D (ActD) and 80S ribosomal protein synthesis inhibitors such as cycloheximide (CHI) inhibit enhancement by indole 3-acetic acid (IAA) of the activity of the cell wall biosynthetic enzyme, glucan synthase I (GS). This supersedes earl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.2.523

    authors: Ray PM

    更新日期:1987-10-01 00:00:00

  • Inhibition of leaf process by p-fluorophenylalanine during induction of flowering in the cocklebur.

    abstract::The amino acid antimetabolite, DL-p-fluorophenylalanine (FPA), inhibited induction of flowering in the short-day cocklebur plant, Xanthium pensylvanicum Wall., primarily by interfering with processes occurring during the inductive dark period. At the concentrations used the inhibitor had little effect on subsequent ve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.7.1185

    authors: Miller J,Ross C

    更新日期:1966-09-01 00:00:00

  • Regulation of ribulose-1,5-bisphosphate carboxylase expression in second leaves of maize seedlings from low and high yield populations.

    abstract::Ribulose-1,5-bisphosphate carboxylase oxygenase (EC 4.1.1.39) (Rubisco) activity, Rubisco-protein, and Rubisco large and small subunit gene (rbcL and rbcS) transcripts were measured at seven stages of development in the second leaf of maize (Zea mays L.) seedlings belonging to low and high yield populations. During th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.541

    authors: Loza-Tavera H,Martínez-Barajas E,Sánchez-de-Jiménez E

    更新日期:1990-06-01 00:00:00

  • Specific reduction of wheat storage proteins by thioredoxin h.

    abstract::Gliadins and glutenins, the major storage proteins of wheat endosperm (Triticum durum, Desf. cv Monroe), were reduced in vitro by the NADP/thioredoxin system (NADPH, NADP-thioredoxin reductase and thioredoxin; in plants, the h type) from either the same source or the bacterium Escherichia coli. A more limited reducti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.3.919

    authors: Kobrehel K,Wong JH,Balogh A,Kiss F,Yee BC,Buchanan BB

    更新日期:1992-07-01 00:00:00

  • Analysis of Variability in the Amaranthus Bioassay for Cytokinins: Effects of Water Stress on Benzyladenine- and Fusicoccin-dependent Responses.

    abstract::The use of only the upper part of the hypocotyl and the cotyledons in the Amaranthus tricolor bioassay for cytokinins, instead of the whole seedling, was found to reduce the endogenous response and give a higher benzyladenine-dependent response. There is no marked difference in the uptake or metabolism of benzyladenin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.2.269

    authors: Elliott DC

    更新日期:1979-02-01 00:00:00

  • The mechanic state of "inner tissue" in the growing zone of sunflower hypocotyls and the regulation of its growth rate following excision.

    abstract::Spontaneous growth of isolated inner tissue from the etiolated sunflower (Helianthus annuus L.) hypocotyl growing zone was investigated. A new preparation technique allowed measurements starting 3 s after excision. Elongation with respect to the turgescent and plasmolized state was quantified in terms of relative grow...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.2.605

    authors: Peters WS,Tomos AD

    更新日期:2000-06-01 00:00:00

  • LTR_retriever: A Highly Accurate and Sensitive Program for Identification of Long Terminal Repeat Retrotransposons.

    abstract::Long terminal repeat retrotransposons (LTR-RTs) are prevalent in plant genomes. The identification of LTR-RTs is critical for achieving high-quality gene annotation. Based on the well-conserved structure, multiple programs were developed for the de novo identification of LTR-RTs; however, these programs are associated...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01310

    authors: Ou S,Jiang N

    更新日期:2018-02-01 00:00:00

  • New lv Mutants of Pea Are Deficient in Phytochrome B.

    abstract::The lv-1 mutant of pea (Pisum sativum L.) is deficient in responses regulated by phytochrome B (phyB) in other species but has normal levels of spectrally active phyB. We have characterized three further lv mutants (lv-2, lv-3, and lv-4), which are all elongated under red (R) and white light but are indistinguishable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.525

    authors: Weller JL,Nagatani A,Kendrick RE,Murfet IC,Reid JB

    更新日期:1995-06-01 00:00:00

  • Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation.

    abstract::Plant architecture is determined by genetic and developmental programs as well as by environmental factors. Sessile plants have evolved a subtle adaptive mechanism that allows them to alter their growth and development during periods of stress. Phytohormones play a central role in this process; however, the molecules ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.146803

    authors: Zhang SW,Li CH,Cao J,Zhang YC,Zhang SQ,Xia YF,Sun DY,Sun Y

    更新日期:2009-12-01 00:00:00

  • Both subunits of the circadian RNA-binding protein CHLAMY1 can integrate temperature information.

    abstract::The circadian RNA-binding protein CHLAMY1 from the green alga Chlamydomonas reinhardtii consists of two subunits named C1 and C3. Changes in the C1 level cause arrhythmicity of the phototaxis rhythm, while alterations in the level of C3 lead to acrophase shifts. Thus, CHLAMY1 is involved in maintaining period and phas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.118570

    authors: Voytsekh O,Seitz SB,Iliev D,Mittag M

    更新日期:2008-08-01 00:00:00

  • Purification and Properties of the Constitutive Arginase of Evernia prunastri.

    abstract::Constitutive arginase (molecular weight 330,000) 920-fold purified from Evernia prunastri thallus, is activated by putrescine, l-ornithine, and agmatine with K(a) values of 2.7, 1.1, and 5.8 millimolar, respectively. Constitutive arginase is also activated by endogenous l-arginine, reaching its maximum activity at 16 ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.4.1065

    authors: Martín-Falquina A,Legaz ME

    更新日期:1984-12-01 00:00:00

  • Effect of asymmetric auxin application on Helianthus hypocotyl curvature.

    abstract::Indole-3-acetic acid was applied asymmetrically to the hypocotyls of sunflower (Helianthus annuus L.) seedlings. After 5 hours on a clinostat, auxin gradients as small as 1 to 1.3 produced substantial (more than 60 degrees) hypocotyl curvature. This result suggests the asymmetric growth underlying hypocotyl gravitro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.2.466

    authors: Migliaccio F,Rayle DL

    更新日期:1989-01-01 00:00:00

  • Enzymic Analysis of Feruloylated Arabinoxylans (Feraxan) Derived from Zea mays Cell Walls I : Purification of Novel Enzymes Capable of Dissociating Feraxan Fragments from Zea mays Coleoptile Cell Wall.

    abstract::Three novel beta-xylan xylanohydrolases capable of dissociating ferulated arabinoxylan (Feraxan) from maize (Zea mays L. hybrid B73 x Mo17) coleoptile sections and two conventional beta-xylan xylanohydrolases (xylanases) were purified from a Bacillus subtilis industrial enzyme preparation (Novo Ban L-120). The Feraxan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.4.883

    authors: Nishitani K,Nevins DJ

    更新日期:1988-08-01 00:00:00

  • beta-Glucosidase Activity in Corn Roots: Problems in Subcellular Fractionation.

    abstract::Preliminary results from differential centrifugation experiments, washing treatments, and enrichment in linear sucrose gradients at a density of 1.09 grams per cubic centimeter all indicated that beta-glucosidase activity in corn root homogenates was associated with a membrane such as tonoplast. A subsequent sucrose d...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.4.861

    authors: Nagahashi G,Baker AF

    更新日期:1984-12-01 00:00:00