Storage Protein Synthesis during Oat (Avena sativa L.) Seed Development.

Abstract:

:Oat (Avena sativa L.) seeds harvested at 2-day intervals from anthesis to maturity were tested for their ability to incorporate [(35)S]sulfate into protein. Incorporation of [(35)S]sulfate into TCA-insoluble material began 2 to 4 days postanthesis (DPA), reached a peak 14 to 16 DPA, and was barely detectable by 24 DPA. Incorporation of label into globulin was parallel to total protein accumulation, and averaged about 85% of the total protein synthesis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total protein extracted from developing seeds indicated that some polypeptides coinciding with the alpha and beta globulin subunits were present 2 to 4 DPA, but the full complement of globulin polypeptides was not present until 10 DPA. Immunoprecipitation of in vivo labeled seed extracts showed that globulin polypeptides and the 59 kilodalton precursor were present at early stages of development (4 DPA). Quantitation of dot blot analysis, using an oat globulin cDNA clone as a probe, indicated that one species of oat globulin mRNA was most abundant 15 DPA, which is during the peak time of storage protein synthesis.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Luthe DS

doi

10.1104/pp.84.2.337

subject

Has Abstract

pub_date

1987-06-01 00:00:00

pages

337-40

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

84

pub_type

杂志文章
  • Developmental and environmental regulation of the Nicotiana plumbaginifolia cytosolic Cu/Zn-superoxide dismutase promoter in transgenic tobacco.

    abstract::Superoxide dismutases (SODs) play a key role in the cellular defense against reactive oxygen species. To study the transcriptional regulation at the cellular level, the promoter of the Nicotiana plumbaginifolia cytosolic gene encoding Cu/ZnSOD (SODCc) was fused to the beta-glucuronidase (GUS) reporter gene (gusA) and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.3.873

    authors: Hérouart D,Van Montagu M,Inzé D

    更新日期:1994-03-01 00:00:00

  • A CCCH-type zinc finger nucleic acid-binding protein quantitatively confers resistance against rice bacterial blight disease.

    abstract::Bacterial blight is a devastating disease of rice (Oryza sativa) caused by Xanthomonas oryzae pv oryzae (Xoo). Zinc finger proteins harboring the motif with three conserved cysteine residues and one histidine residue (CCCH) belong to a large family. Although at least 67 CCCH-type zinc finger protein genes have been id...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.191379

    authors: Deng H,Liu H,Li X,Xiao J,Wang S

    更新日期:2012-02-01 00:00:00

  • Embryo Development in Phaseolus vulgaris: II. Analysis of Selected Inorganic Ions, Ammonia, Organic Acids, Amino Acids, and Sugars in the Endosperm Liquid.

    abstract::Endosperm liquid bathes the embryo of Phaseolus vulgaris from the heart stage through the late cotyledon state. This liquid was aspirated from many ovules of the same stage, pooled, and analyzed for the following constituents and parameters: [List: see text]. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.3.454

    authors: Smith JG

    更新日期:1973-03-01 00:00:00

  • Loss of Ribulose 1,5-Diphosphate Carboxylase and Increase in Proteolytic Activity during Senescence of Detached Primary Barley Leaves.

    abstract::Symptoms typical of senescence occurred in green detached primary barley (Hordeum vulgare L.) leaves placed in darkness and in light. Chlorophyll, total soluble protein, ribulose 1,5-diphosphate carboxylase protein and activity each progressively decreased in darkness and to a lesser extent in light. In all treatments...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.6.1009

    authors: Peterson LW,Huffaker RC

    更新日期:1975-06-01 00:00:00

  • Specificity for Nicotinamide Adenine Dinucleotide and Nicotinamide Adenine Dinucleotide Phosphate of Nitrate Reductase from the Salt-tolerant Alga Dunaliella parva.

    abstract::Nitrate reductase of the salt-tolerant alga Dunaliella parva could utilize NADPH as well as NADH as an electron donor. The two pyridine nucleotide-dependent activities could not be separated by either ion exchange chromatography on DEAE-cellulose or gel filtration on Sepharose 4B. The NADPH-dependent activity was not ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.1.57

    authors: Heimer YM

    更新日期:1976-07-01 00:00:00

  • Alteration of soybean complex lipid biosynthesis by s-ethyl dipropylthiocarbamate.

    abstract::Soybean (Glycine max [L.] Merr. var. Glabrous D62-7812) plants were grown in aerated Hoagland and Arnon mineral nutrient solution containing 0 or 2.6 mum S-ethyl dipropylthiocarbamate (EPTC) in a growth chamber. After 19 days exposure to EPTC, total leaf fresh weight was reduced 18% by 2.6 mum EPTC while total leaf fa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.1.86

    authors: Wilkinson RE,Smith AE

    更新日期:1977-07-01 00:00:00

  • Calcium transport in tonoplast and endoplasmic reticulum vesicles isolated from cultured carrot cells.

    abstract::Two active calcium (Ca(2+)) transport systems have been identified and partially characterized in membrane vesicles isolated from cultured carrot cells (Daucus carota Danvers). Both transport systems required MgATP for activity and were enhanced by 10 millimolar oxalate. Ca(2+) transport in membrane vesicles derived f...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.2.549

    authors: Bush DR,Sze H

    更新日期:1986-02-01 00:00:00

  • Root Exudates of Various Host Plants of Rhizobium leguminosarum Contain Different Sets of Inducers of Rhizobium Nodulation Genes.

    abstract::Rhizobium promoters involved in the formation of root nodules on leguminous plants are activated by flavonoids in plant root exudate. A series of Rhizobium strains which all contain the inducible Rhizobium leguminosarum nodA promoter fused to the Escherichia coli lacZ gene, and which differ only in the source of the r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.4.1298

    authors: Zaat SA,Wijffelman CA,Mulders IH,van Brussel AA,Lugtenberg BJ

    更新日期:1988-04-01 00:00:00

  • Direct evidence for rapid degradation of Bacillus thuringiensis toxin mRNA as a cause of poor expression in plants.

    abstract::It is well established that the expression of Bacillus thuringiensis (B.t.) toxin genes in higher plants is severely limited at the mRNA level, but the cause remains controversial. Elucidating whether mRNA accumulation is limited transcriptionally or posttranscriptionally could contribute to effective gene design as w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.4.1445

    authors: De Rocher EJ,Vargo-Gogola TC,Diehn SH,Green PJ

    更新日期:1998-08-01 00:00:00

  • Interactions between Mild NaCl Stress and Red Light during Lettuce (Lactuca sativa L. cv Grand Rapids) Seed Germination.

    abstract::The sensitivity of lettuce (Lactuca sativa L. cv Grand Rapids) seeds to red light was reduced by NaCl concentrations which had no effect upon the germination of continuously illuminated seeds. The germination capacity of the seeds was fully restored by increased red light exposures. Indirect evidence indicates that Na...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.1.149

    authors: Scorer KN,Epel BL,Waisel Y

    更新日期:1985-09-01 00:00:00

  • Procedures for the Generation of Mature Chlamydomonas reinhardtii Zygotes for Molecular and Biochemical Analyses.

    abstract::Zygotes represent an important stage in the sexual cycle of the unicellular green alga Chlamydomonas reinhardtii. To study zygote germination at a molecular level, a protocol was elaborated for the generation of zygotes in large quantities and a method was developed for the extraction from zygotes of RNA that could be...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.2.512

    authors: Wegener D,Treier U,Beck CF

    更新日期:1989-06-01 00:00:00

  • Selective enhancement of alternative path capacity in plant storage organs in response to ethylene plus oxygen: a comparative study.

    abstract::The respiration rise in bulky storage organs induced by ethylene plus pure O(2) is accompanied by an increase in the CN-resistant respiration, or alternative path. Whereas a lesser respiratory stimulation in response to ethylene is demonstrable in air and increased by peeling, ethylene-induced alternative path develop...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.5.1036

    authors: Theologis A,Laties GG

    更新日期:1982-05-01 00:00:00

  • Role of roots in regulating the growth rate and cytokinin content in leaves.

    abstract::The role of roots in the enhancement of cytokinin content and leaf growth of Phaseolus vulgaris plants after decapitation and partial defoliation was investigated. Partial excision of the roots of plants which were decapitated above the primary leaf node resulted in a reduction of leaf growth and soluble proteins accu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.1.76

    authors: Carmi A,Staden JV

    更新日期:1983-09-01 00:00:00

  • Relationship between Freezing Tolerance of Root-Tip Cells and Cold Stability of Microtubules in Rye (Secale cereale L. cv Puma).

    abstract::The response of cortical microtubules to low temperature and freezing was assessed for root tips of cold-acclimated and non-acclimated winter rye (Secale cereale L. cv Puma) seedlings using indirect immunofluorescence microscopy with antitubulin antibodies. Roots cooled to 0 or -3 degrees C were fixed for immunofluore...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.1.77

    authors: Kerr GP,Carter JV

    更新日期:1990-05-01 00:00:00

  • The rapid isolation of vacuoles from leaves of crassulacean Acid metabolism plants.

    abstract::A technique is presented for the isolation of vacuoles from Sedum telephium L. leaves. Leaf material is digested enzymically to produce protoplasts rapidly which are partially lysed by gentle osmotic shock and the inclusion of 5 millimolar ethyleneglycol-bis (beta-aminoethyl ether)N,N'-tetraacetic acid in the wash med...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.3.379

    authors: Kringstad R,Kenyon WH,Black CC

    更新日期:1980-09-01 00:00:00

  • Fructose 2,6-bisphosphate inhibition of phosphoglucomutase.

    abstract::Fructose 2,6-bisphosphate inhibits phosphoglucomutase noncompetitively with respect to the cofactor glucose 1,6-bisphosphate. Previous studies from our laboratory had shown that phosphoglucomutase was activated by fructose 2,6-bisphosphate in the absence of added glucose 1,6-bisphosphate. The fructose 2,6-bisphosphate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.4.980

    authors: Galloway CM,Dugger WM,Black CC

    更新日期:1988-12-01 00:00:00

  • Does ethylene play a role in the release of lateral buds (tillers) from apical dominance in oats?

    abstract::The growth of lateral buds (tillers), which are undergoing release from apical dominance, was measured in upright and gravistimulated intact Avena sativa L. cv. ;Victory' (oat) shoots as well as in isolated Avena stem segments treated with kinetin and sucrose. During release, the tiller bud initially shows a slow rate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.3.811

    authors: Harrison MA,Kaufman PB

    更新日期:1982-09-01 00:00:00

  • Disruption of the actin cytoskeleton results in the promotion of gravitropism in inflorescence stems and hypocotyls of Arabidopsis.

    abstract::The actin cytoskeleton is hypothesized to play a major role in gravity perception and transduction mechanisms in roots of plants. To determine whether actin microfilaments (MFs) are involved in these processes in stem-like organs, we studied gravitropism in Arabidopsis inflorescence stems and hypocotyls. Localization ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010804

    authors: Yamamoto K,Kiss JZ

    更新日期:2002-02-01 00:00:00

  • Metabolism of Indole-3-Acetic Acid: III. Identification of Metabolites Isolated from Crown Gall Callus Tissue.

    abstract::The metabolism of labeled indole-3-acetic acid (IAA-2-(14)C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were asp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.5.666

    authors: Feung CS,Hamilton RH,Mumma RO

    更新日期:1976-11-01 00:00:00

  • Stoichiometry of Photosystem I, Photosystem II, and Phycobilisomes in the Red Alga Porphyridium cruentum as a Function of Growth Irradiance.

    abstract::Cells of the red alga Porphyridium cruentum (ATCC 50161) exposed to increasing growth irradiance exhibited up to a three-fold reduction in photosystems I and II (PSI and PSII) and phycobilisomes but little change in the relative numbers of these components. Batch cultures of P. cruentum were grown under four photon fl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.3.1179

    authors: Cunningham FX,Dennenberg RJ,Mustardy L,Jursinic PA,Gantt E

    更新日期:1989-11-01 00:00:00

  • Two homologous low-temperature-inducible genes from Arabidopsis encode highly hydrophobic proteins.

    abstract::We have characterized two related cDNAs (RCI2A and RCI2B) corresponding to genes from Arabidopsis thaliana, the expression of which is transiently induced by low, nonfreezing temperatures. RCI2A and RCI2B encode small (54 amino acids), highly hydrophobic proteins that bear two potential transmembrane domains. They sho...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.2.569

    authors: Capel J,Jarillo JA,Salinas J,Martínez-Zapater JM

    更新日期:1997-10-01 00:00:00

  • The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro

    abstract::The effects of plant hormones and sucrose (Suc) on potato (Solanum tuberosum L.) tuberization were studied using in vitro cultured single-node cuttings. Tuber-inducing (high Suc) and -noninducing (low Suc or high Suc plus gibberellin [GA]) media were tested. Tuberization frequencies, tuber widths, and stolon lengths w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.2.575

    authors: Xu X,van Lammeren AA,Vermeer E,Vreugdenhil D

    更新日期:1998-06-01 00:00:00

  • Lignification in sugarcane: biochemical characterization, gene discovery, and expression analysis in two genotypes contrasting for lignin content.

    abstract::Sugarcane (Saccharum spp.) is currently one of the most efficient crops in the production of first-generation biofuels. However, the bagasse represents an additional abundant lignocellulosic resource that has the potential to increase the ethanol production per plant. To achieve a more efficient conversion of bagasse ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.225250

    authors: Bottcher A,Cesarino I,Santos AB,Vicentini R,Mayer JL,Vanholme R,Morreel K,Goeminne G,Moura JC,Nobile PM,Carmello-Guerreiro SM,Anjos IA,Creste S,Boerjan W,Landell MG,Mazzafera P

    更新日期:2013-12-01 00:00:00

  • Effects of inorganic phosphate on the light dependent thylakoid energization of intact spinach chloroplasts.

    abstract::The light dependent energization of the thylakoid membrane was analyzed in isolated intact spinach (Spinacia oleracea L.) chloroplasts incubated with different concentrations of inorganic phosphate (Pi). Two independent methods were used: (a) the accumulation of [(14)C]5,5-dimethyl-2,4-oxazolidinedione and [(14)C] met...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.1.221

    authors: Heineke D,Stitt M,Heldt HW

    更新日期:1989-09-01 00:00:00

  • Chemical composition of the Prunus laurocerasus leaf surface. Dynamic changes of the epicuticular wax film during leaf development.

    abstract::The seasonal development of adaxial Prunus laurocerasus leaf surfaces was studied using newly developed methods for the mechanical removal of epicuticular waxes. During epidermal cell expansion, more than 50 microg leaf(-1) of alkyl acetates accumulated within 10 d, forming an epicuticular wax film approximately 30 nm...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.4.1725

    authors: Jetter R,Schäffer S

    更新日期:2001-08-01 00:00:00

  • Gains and Losses of Cis-regulatory Elements Led to Divergence of the Arabidopsis APETALA1 and CAULIFLOWER Duplicate Genes in the Time, Space, and Level of Expression and Regulation of One Paralog by the Other.

    abstract::How genes change their expression patterns over time is still poorly understood. Here, by conducting expression, functional, bioinformatic, and evolutionary analyses, we demonstrate that the differences between the Arabidopsis (Arabidopsis thaliana) APETALA1 (AP1) and CAULIFLOWER (CAL) duplicate genes in the time, spa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00320

    authors: Ye L,Wang B,Zhang W,Shan H,Kong H

    更新日期:2016-06-01 00:00:00

  • Direct measurement of calcium transport across chloroplast inner-envelope vesicles

    abstract::The initial rate of Ca2+ movement across the inner-envelope membrane of pea (Pisum sativum L.) chloroplasts was directly measured by stopped-flow spectrofluorometry using membrane vesicles loaded with the Ca2+-sensitive fluorophore fura-2. Calibration of fura-2 fluorescence was achieved by combining a ratiometric meth...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.4.1447

    authors: Roh MH,Shingles R,Cleveland MJ,McCarty RE

    更新日期:1998-12-01 00:00:00

  • Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen.

    abstract::Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate (I-1,2,3,4,5,6-P6). A number of phytases with varying specificities, properties, and localizations hydrolyze phytic acid present in cells. The specificity of hydrolysis of phytic acid by alkaline p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.4.1489

    authors: Barrientos L,Scott JJ,Murthy PP

    更新日期:1994-12-01 00:00:00

  • The last step in cocaine biosynthesis is catalyzed by a BAHD acyltransferase.

    abstract::The esterification of methylecgonine (2-carbomethoxy-3β-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase. The enzyme is capabl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.248187

    authors: Schmidt GW,Jirschitzka J,Porta T,Reichelt M,Luck K,Torre JC,Dolke F,Varesio E,Hopfgartner G,Gershenzon J,D'Auria JC

    更新日期:2015-01-01 00:00:00

  • Flow cytometry of spinach chloroplasts : determination of intactness and lectin-binding properties of the envelope and the thylakoid membranes.

    abstract::Intact spinach (Spinacia oleracea) chloroplasts, thylakoid membranes, and inside-out or right-side-out thylakoid vesicles have been characterized by flow cytometry with respect to forward angle light scatter, right angle light scatter, and chlorophyll fluorescence. Analysis of intact chloroplasts with respect to forwa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.3.1092

    authors: Schröder WP,Petit PX

    更新日期:1992-11-01 00:00:00