Direct measurement of calcium transport across chloroplast inner-envelope vesicles

abstract：:The proton transport properties of plasma membrane and tonoplast vesicles isolated from red beet (Beta vulgaris L.) storage tissue were examined and compared. Membrane vesicles isolated with 250 millimolar KCl in the homogenization media and recovered at low density following sucrose density gradient centrifugation di...

journal_title：Plant physiology

authors： Giannini JL,Briskin DP

更新日期：1987-07-01 00:00:00

abstract：:Flowering of the facultative long-day plant Arabidopsis is controlled by several endogenous and environmental factors, among them gibberellins (GAs) and day length. The promotion of flowering by long days involves an endogenous clock that interacts with light cues provided by the environment. Light, and specifically p...

journal_title：Plant physiology

authors： Blázquez MA,Trénor M,Weigel D

更新日期：2002-12-01 00:00:00

abstract：:Auxin-deprived, mannitol-supplemented, suspension-cultured pear (Pyrus communis L. Passe Crassane) fruit cells produce large quantities (20-40 nanoliters ethylene per 10(6) cells per hour) of ethylene in response to auxins, CuCl(2) or 1-amino-cyclopropane-1-carboxylic acid (ACC). Maximum rates of production are achiev...

journal_title：Plant physiology

authors： Puschmann R,Romani R

更新日期：1983-12-01 00:00:00

abstract：:Evidence is presented that intracellular ammonium is trapped in vacuoles of maize (Zea mays L.) root tips because of rapid movement of ammonia between cytoplasm and vacuoles. The concentration of cytoplasmic ammonium is estimated to be <15 mum at extracellular ammonium concentrations up to 1 mm. The implications for p...

journal_title：Plant physiology

authors： Roberts JK,Pang MK

更新日期：1992-11-01 00:00:00

abstract：:It has been suggested by others that the spectrophotometric assay of phenylalanine ammonia-lyase based on changes in absorbance at 290 nm may be complicated by a side reaction involving transamination from phenylalanine onto alpha-keto acids. This would lead to the production of phenylpyruvate which would spontaneousl...

journal_title：Plant physiology

authors： Saunders JA,McClure JW

更新日期：1974-09-01 00:00:00

abstract：:Evidence for photosynthetic linear electron transport in guard cells was obtained with two sensitive methods of high spacial resolution. Light-dependent diaminobenzidine oxidation (an indicator of PSI) and DCMU-sensitive, light-dependent thiocarbamyl nitroblue tetrazolium reduction (an indicator of PSII) were observed...

journal_title：Plant physiology

authors： Vaughn KC,Outlaw WH

更新日期：1983-02-01 00:00:00

abstract：:Previous work indicated more polysomes bound to pea (Pisum sativum cv Progress No. 9) thylakoids in light than in the dark, in vivo (LE Fish, AT Jagendorf 1982 Plant Physiol 69: 814-825). With isolated intact chloroplasts incubated in darkness, addition of MgATP had no effect but 24 to 74% more RNA was thylakoid-bound...

journal_title：Plant physiology

authors： Hurewitz J,Jagendorf AT

更新日期：1987-05-01 00:00:00

abstract：:Nonautotrophic CO(2) metabolism in Opuntia echinocarpa roots was studied with techniques of manometry and radiometry. The roots were grown in a one-quarter strength nutrient solution for several days; the distal 2 cm was used for physiological studies. The roots assimilated significant quantities of (14)CO(2) and appe...

journal_title：Plant physiology

authors： Ting IP,Dugger WM

更新日期：1966-03-01 00:00:00

abstract：:White light (25 watts per square meter) induced an increase in plasma membrane K(+)-channel activity and a 30- to 70-millivolt transient membrane depolarization (completed in 2-3 minutes) in Arabidopsis thaliana leaf mesophyll cells. Transport characteristics of three types of ion channels in the plasma membrane were ...

journal_title：Plant physiology

authors： Spalding EP,Slayman CL,Goldsmith MH,Gradmann D,Bertl A

更新日期：1992-05-01 00:00:00

abstract：:Light-induced conversion of NAD to NADP was investigated in higher plants. Upon illumination, conversion of NAD to NADP was observed in intact leaves of wheat and pea following incubation in the dark. This conversion was also observed in mesophyll protoplasts of wheat leaves when they were isolated in the dark or isol...

journal_title：Plant physiology

authors： Muto S,Miyachi S

更新日期：1981-08-01 00:00:00

abstract：:Polyamines are small ubiquitous molecules that have been involved in nearly all developmental processes, including the stress response. Nevertheless, no direct evidence of a role of polyamines in the wound response has been described. We have studied the expression of genes involved in polyamine biosynthesis in respon...

journal_title：Plant physiology

authors： Perez-Amador MA,Leon J,Green PJ,Carbonell J

更新日期：2002-11-01 00:00:00

abstract：:The occurrence of proteins able to oxidize polyphenols even in the absence of H2O2 was recently reported in mung bean (Vigna radiata L.) hypocotyl cell wall extracts (R. Goldberg, A. Chabanet, A.M. Catesson [1993] In K.G. Welinder, S.K. Rasmussen, C. Penel, H. Greppin, eds, Plant Peroxidases: Biochemistry and Physiolo...

journal_title：Plant physiology

authors： Chabanet A,Goldberg R,Catesson AM,Quinet-Szely M,Delaunay AM,Faye L

更新日期：1994-11-01 00:00:00

abstract：:We initially compared lipid peroxidation profiles in tobacco (Nicotiana tabacum) leaves during different cell death events. An upstream oxylipin assay was used to discriminate reactive oxygen species (ROS)-mediated lipid peroxidation from 9- and 13-lipoxygenase (LOX)-dependent lipid peroxidation. Free radical-mediated...

journal_title：Plant physiology

authors： Montillet JL,Chamnongpol S,Rustérucci C,Dat J,van de Cotte B,Agnel JP,Battesti C,Inzé D,Van Breusegem F,Triantaphylidès C

更新日期：2005-07-01 00:00:00

abstract：:Three populations of seedlings of Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) were subjected to recurrent drought cycles. Pressure-volume curves were developed to derive the osmotic and pressure potentials as functions of the symplastic water over a wide range of relative water content. The drought cycles induc...

journal_title：Plant physiology

authors： Joly RJ,Zaerr JB

更新日期：1987-02-01 00:00:00

abstract：:The localization of plasma membrane H(+)-ATPase has been studied at the optical microscope level utilizing frozen and paraffin sections of Avena sativa and Pisum sativum, specific anti-ATPase polyclonal antibody, and second antibody coupled to alkaline phosphatase. In leaves and stems the ATPase is concentrated at the...

journal_title：Plant physiology

authors： Parets-Soler A,Pardo JM,Serrano R

更新日期：1990-08-01 00:00:00

abstract：:Phosphate in the xylem exudate of tomato (Lycopersicon esculentum) plants was 70 to 98% inorganic phosphate (Pi), 2 to 30% P-choline, and less than 1% P-ethanolamine. Upon adding (32)Pi to the nutrient, Pi in xylem exudate had the same specific activity within 4 hours. P-choline and P-ethanolamine reached the same spe...

journal_title：Plant physiology

authors： Martin BA,Tolbert NE

更新日期：1983-10-01 00:00:00

abstract：:Although sessile in nature, plants are able to use a number of mechanisms to modify their morphology in response to changing environmental conditions. Differential growth is one such mechanism. Despite its importance in plant development, little is known about the molecular events regulating the establishment of diffe...

journal_title：Plant physiology

authors： Stowe-Evans EL,Harper RM,Motchoulski AV,Liscum E

更新日期：1998-12-01 00:00:00

abstract：:Heat shock factors (HSFs) are transcriptional regulators of the heat shock response. The major target of HSFs are the genes encoding heat shock proteins (HSPs), which are known to have a protective function that counteracts cytotoxic effects. To identify other HSF target genes, which may be important determinants for ...

journal_title：Plant physiology

authors： Panikulangara TJ,Eggers-Schumacher G,Wunderlich M,Stransky H,Schöffl F

更新日期：2004-10-01 00:00:00

abstract：:A new conjugate, 1-(gamma-L-glutamylamino)cyclopropane-1-carboxylic acid (GACC), of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is identified. The only previously identified conjugate of ACC is 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). GACC, not MACC, was the major conjugate formed ...

journal_title：Plant physiology

authors： Martin MN,Cohen JD,Saftner RA

更新日期：1995-11-01 00:00:00

abstract：:When (32)P(1) was supplied as a 15-minute pulse to normal Spirodela oligorrhiza plants, the first phospholipid to become fully labeled was phosphatidic acid. Phosphatidyl glycerol reached maximum labeling before the other major phospholipids. In phosphorus-deficient plants, however, phosphatidyl glycerol became labele...

journal_title：Plant physiology

authors： Bieleski RL

更新日期：1972-05-01 00:00:00

abstract：:The effects of representative respiratory inhibitors were investigated on the coupled respiration of mung bean mitochondria using succinate and l-malate as substrates. The inhibitors studied were: (I) malonate, (II) amytal and rotenone, (III) antimycin A and 2-n-nonyl-4-hydroxyquinoline N-oxide (NOQNO), and (IV) cyani...

journal_title：Plant physiology

authors： Ikuma H,Bonner WD

更新日期：1967-11-01 00:00:00

abstract：:Membrane preparation (sedimenting between 13,000(g) and 80,000(g)) of germinating radish seeds (Raphanus sativus L.) was active in hydrolyzing ATP and, to a lesser extent, a variety of other phosphorylated compounds. Dicyclohexylcarbodiimide (DCCD) and diethylstilbestrol significantly inhibited the ATPase activity (40...

journal_title：Plant physiology

authors： Cocucci M,Ballarin-Denti A

更新日期：1981-08-01 00:00:00

abstract：:Mitochondria isolated from Kalanchoë daigremontiana, a Crassulacean acid metabolism plant, decarboxylate added malate to pyruvate at rates of up to 100 micromoles per hour per milligram original chlorophyll in the presence of ADP. Omitting ADP reduces this rate by approximately 50%. Antimycin A inhibits malate decarbo...

journal_title：Plant physiology

authors： Day DA

更新日期：1980-04-01 00:00:00

abstract：:In this article, we demonstrate the connection between intracellular iron storage and oxidative stress response in cyanobacteria. Iron is essential for the survival of all organisms. However, the redox properties that make iron a valuable cofactor also lead to oxidative interactions, resulting in the formation of harm...

journal_title：Plant physiology

authors： Shcolnick S,Summerfield TC,Reytman L,Sherman LA,Keren N

更新日期：2009-08-01 00:00:00

abstract：:The effects of CO2 enrichment on growth and N allocation of rice (Oryza sativa L.) were examined. The plants were grown hydroponically in growth chambers with a 14-h photoperiod (1000 [mu]mol quanta m-2 s-1) and a day/night temperature of 25/20[deg]C. From the 28th to 70th d after germination, the plants were exposed ...

journal_title：Plant physiology

authors： Makino A,Harada M,Sato T,Nakano H,Mae T

更新日期：1997-09-01 00:00:00

abstract：:Filaments of the blue-green alga Anabaena variabilis permeabilized by dimethylsulfoxide (DMSO) produce increased amounts of 5-aminolevulinate in the presence of levulinic acid. The metabolic activity of the filaments remains unperturbed in the presence of up to 7.5% (v/v) DMSO. Studies utilizing DMSO-permeabilized fil...

journal_title：Plant physiology

authors： Avissar YJ

更新日期：1983-05-01 00:00:00

abstract：:Serial analysis of gene expression was used to profile transcript levels in Arabidopsis roots and assess their responses to 2,4,6-trinitrotoluene (TNT) exposure. SAGE libraries representing control and TNT-exposed seedling root transcripts were constructed, and each was sequenced to a depth of roughly 32,000 tags. Mor...

journal_title：Plant physiology

authors： Ekman DR,Lorenz WW,Przybyla AE,Wolfe NL,Dean JF

更新日期：2003-11-01 00:00:00

abstract：:In Arabidopsis (Arabidopsis thaliana) the 466 pentatricopeptide repeat (PPR) proteins are putative RNA-binding proteins with essential roles in organelles. Roughly half of the PPR proteins form the plant combinatorial and modular protein (PCMP) subfamily, which is land-plant specific. PCMPs exhibit a large and variabl...

journal_title：Plant physiology

authors： Rivals E,Bruyère C,Toffano-Nioche C,Lecharny A

更新日期：2006-07-01 00:00:00

abstract：:The inhibitory activities of amatoxins on the growth of Chlamydomonas reinhardtii have been determined using a convenient assay based upon incubation in multiwell tissue culture plates followed by turbidimetric estimates of growth on a multiwell plate reader. Values for the inhibitory dosage at which growth is 50% of ...

journal_title：Plant physiology

authors： Dusek DM,Preston JF

更新日期：1988-05-01 00:00:00

abstract：:CTF7 is an essential gene in yeast that is required for the formation of sister chromatid cohesion. While recent studies have provided insights into how sister chromatid cohesion is established, less is known about how specifically CTF7 facilitates the formation of cohesion, and essentially nothing is known about how ...

journal_title：Plant physiology

authors： Jiang L,Yuan L,Xia M,Makaroff CA

更新日期：2010-10-01 00:00:00