Abstract:
:Serial analysis of gene expression was used to profile transcript levels in Arabidopsis roots and assess their responses to 2,4,6-trinitrotoluene (TNT) exposure. SAGE libraries representing control and TNT-exposed seedling root transcripts were constructed, and each was sequenced to a depth of roughly 32,000 tags. More than 19,000 unique tags were identified overall. The second most highly induced tag (27-fold increase) represented a glutathione S-transferase. Cytochrome P450 enzymes, as well as an ABC transporter and a probable nitroreductase, were highly induced by TNT exposure. Analyses also revealed an oxidative stress response upon TNT exposure. Although some increases were anticipated in light of current models for xenobiotic metabolism in plants, evidence for unsuspected conjugation pathways was also noted. Identifying transcriptome-level responses to TNT exposure will better define the metabolic pathways plants use to detoxify this xenobiotic compound, which should help improve phytoremediation strategies directed at TNT and other nitroaromatic compounds.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Ekman DR,Lorenz WW,Przybyla AE,Wolfe NL,Dean JFdoi
10.1104/pp.103.028019subject
Has Abstractpub_date
2003-11-01 00:00:00pages
1397-406issue
3eissn
0032-0889issn
1532-2548pii
pp.103.028019journal_volume
133pub_type
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