Abstract:
:While pepper (Capsicum annuum) is a highly recalcitrant species for genetic transformation studies, plant virus-based vectors can provide alternative and powerful tools for transient regulation and functional analysis of genes of interest in pepper. In this study, we established an effective virus-based vector system applicable for transient gain- and loss-of-function studies in pepper using Broad bean wilt virus2 (BBWV2). We engineered BBWV2 as a dual gene expression vector for simultaneous expression of two recombinant proteins in pepper cells. In addition, we established enhanced and stable expression of recombinant proteins from the BBWV2-based dual vector via coexpression of a heterologous viral suppressor of RNA silencing. We also developed a BBWV2-based virus-induced gene silencing (VIGS) vector, and we successfully silenced the phytoene desaturase gene (PDS) using the BBWV2-based VIGS vector in various pepper cultivars. Additionally, we optimized the BBWV2-based VIGS system in pepper by testing the efficiency of PDS gene silencing under different conditions. This BBWV2-based vector system represents a convenient approach for rapid and simple analysis of gene functions in pepper.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Choi B,Kwon SJ,Kim MH,Choe S,Kwak HR,Kim MK,Jung C,Seo JKdoi
10.1104/pp.19.00836subject
Has Abstractpub_date
2019-11-01 00:00:00pages
867-880issue
3eissn
0032-0889issn
1532-2548pii
pp.19.00836journal_volume
181pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::When wheat (Triticum aestivum) seedlings are exposed to a cold temperature (2-4 degrees C) above 0 degrees C, sucrose accumulates and sucrose synthase activity increases. The effect of a cold period on the level of sucrose synthase (SS) was investigated. Using antibodies against wheat germ SS, Western blots studies sh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.3.887
更新日期:1991-07-01 00:00:00
abstract::Recently, we isolated dehydroipomeamarone, a new sesquiterpenoid from sweet potato (Ipomoea batatas Lam.) root tissue infected with Ceratocystis fimbriata (Ell. et Halst.). The purpose of this investigation was to determine whether dehydroipomeamarone was a precursor in the biosynthetic pathway of ipomeamarone. The in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.4.649
更新日期:1974-04-01 00:00:00
abstract::To function, the catalytic sites of Rubisco (EC 4.1.1.39) need to be activated by the reversible carbamylation of a lysine residue within the sites followed by rapid binding of magnesium. The activation of Rubisco in vivo requires the presence of the regulatory protein Rubisco activase. This enzyme is thought to aid t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056077
更新日期:2005-02-01 00:00:00
abstract::The relationship between the state of oligomerization and activity of purified maize leaf phosphoenolpyruvate carboxylase using size exclusion high performance liquid chromatography was examined. Maximum activities of 35 to 38 micromoles per minute per milligram protein were found when 100% of the enzyme was in its te...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.848
更新日期:1986-04-01 00:00:00
abstract::The molecular basis of plant osmosensing remains unknown. Arabidopsis (Arabidopsis thaliana) Histidine Kinase1 (AHK1) can complement the osmosensitivity of yeast (Saccharomyces cerevisiae) osmosensor mutants lacking Synthetic Lethal of N-end rule1 and SH3-containing Osmosensor and has been proposed to act as a plant o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.209791
更新日期:2013-02-01 00:00:00
abstract::Previous experiments using in situ RNA hybridization have shown that the mRNAs encoding phenylalanine ammonia-lyase, 4-coumarate:coenzyme A ligase, and pathogenesis-related protein 1 accumulated transiently around fungal infection sites in parsley (Petroselinum crispum) leaf buds. These studies have now been extended ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.1.131
更新日期:1996-09-01 00:00:00
abstract::Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.1.67
更新日期:2000-01-01 00:00:00
abstract::Under normal field growth conditions, canola (Brassica napus) seeds produce chloroplasts during early seed development and then catabolize the photosynthetic machinery during seed maturation, producing mature seeds at harvest that are essentially free of chlorophyll (Chl). However, frost exposure early in canola seed ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.084483
更新日期:2006-09-01 00:00:00
abstract::The growth of transgenic tomato (Lycopersicon esculentum) plants that express in their apoplast yeast invertase under the control of the cauliflower mosaic virus 35S promoter is severely inhibited. The higher the level of invertase, the greater the inhibition of growth. A second phenotypic characteristic of these tran...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.2.420
更新日期:1991-02-01 00:00:00
abstract::Plants commonly live in a symbiotic association with arbuscular mycorrhizal fungi (AMF). They invest photosynthetic products to feed their fungal partners, which, in return, provide mineral nutrients foraged in the soil by their intricate hyphal networks. Intriguingly, AMF can link neighboring plants, forming common m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.195727
更新日期:2012-06-01 00:00:00
abstract::Using photoacoustic spectroscopy, state 1-state 2 transitions were demonstrated in vivo in intact sugar maple leaves (Acer saccharum Marsh.) by following the changes in energy storage of photosystems (PS) I and II. Energy storage measured with 650 nm modulated light (light 2) in the presence of background white light ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.1.330
更新日期:1991-09-01 00:00:00
abstract::Glutathione is generally accepted as the principal electron donor for dehydroascorbate (DHA) reduction. Moreover, both glutathione and DHA affect cell cycle progression in plant cells. But other mechanisms for DHA reduction have been proposed. To investigate the connection between DHA and glutathione, we have evaluate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.033548
更新日期:2004-04-01 00:00:00
abstract::The sugars in the endosperm of a developing seed have many potential roles, including the supply of carbon to the developing embryo and controlling gene expression in it. Our understanding of their metabolism is, however, fragmentary and is confined to a very few species (especially Vicia spp.). To develop a quantitat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010868
更新日期:2003-01-01 00:00:00
abstract::Simultaneous comparisons were made between a freezing-tolerant, cold-acclimating (CA) wild potato species (Solanum commersonii) and a freezing-sensitive, nonacclimating (NA) cultivated species (Solanum tuberosum). Comparative studies allowed differentiation of plasma membrane lipid changes associated with increased fr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.3.793
更新日期:1993-11-01 00:00:00
abstract::Microarrays measure the expression of large numbers of genes simultaneously and can be used to delve into interaction networks involving many genes at a time. However, it is often difficult to decide to what extent knowledge about the expression of genes gleaned in one model organism can be transferred to other specie...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.109603
更新日期:2008-02-01 00:00:00
abstract::SIRK is a K(+) channel identified in grapevine (Vitis vinifera), belonging to the so-called Shaker family. The highest sequence similarities it shares with the members of this family are found with channels of the KAT type, although SIRK displays a small ankyrin domain. This atypical feature provides a key to understa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010529
更新日期:2002-02-01 00:00:00
abstract::O(2) consumption by the desiccation-tolerant moss Tortula ruralis and the desiccation-intolerant Cratoneuron filicinum increased markedly during the latter stages of desiccation. ATP content of the mosses during desiccation was not correlated with O(2) consumption, but was influenced by the rate at which the mosses lo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.1.13
更新日期:1979-07-01 00:00:00
abstract::One of the two branches of the α-linolenic acid metabolism pathway is catalyzed by 12-oxo-phytodienoic acid reductase I, and the other is involved in jasmonic acid (JA) synthesis. The former is known to be active in the response to salinity tolerance in wheat (Triticum aestivum), but the participation of the latter in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.227595
更新日期:2014-02-01 00:00:00
abstract::First leaves and flag leaves of the wheat species Triticum aestivum cv Anza (6x), T. boeoticum Boiss (2x) L. were examined for content of pyruvate, orthophosphate dikinase (PPDK), phosphoenolpyruvate carboxylase (PEPC), and ribulose 1,5-bisphosphate carboxylase (RuBPC) by protein blot analyses using antibodies to maiz...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.1.96
更新日期:1986-09-01 00:00:00
abstract::Side chains of sugar beet (Beta vulgaris) pectins, which are mainly composed of arabinose (Ara) and galactose (Gal) residues, are esterified by ferulic acid units. Enzymatic hydrolysis of beet cell walls yielded several feruloylated oligosaccharides, which were separated by hydrophobic interaction chromatography. Two ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.035311
更新日期:2004-03-01 00:00:00
abstract::Cell clones were isolated from a population of cultured tomato (Lycopersicon esculentum Mill cv VFNT-cherry) cells and their tolerance to polyethylene glycol (PEG)-induced water stress was measured. Considerable variation for tolerance among the clones was found. Tolerance differences between clones appeared to be spo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.3.645
更新日期:1983-07-01 00:00:00
abstract::Nitrate or ammonium was added to soybean (Glycine max L. Merrill cv Corsoy) plants grown in plastic pouches 10 days after nodules first appeared. By the third day of treatment with 10 millimolar nitrate, nitrogenase specific activity (per unit nodule weight) had decreased to 15% to 25% of that of untreated plants. Lon...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.5.1236
更新日期:1982-11-01 00:00:00
abstract::After incubation for 1 hr with (75)Se-selenate, excised roots of Astragalus crotalariae, a selenium-accumulating species, and A. lentiginosus, a nonaccumulator, had absorbed radioactivity to levels well over the external concentration. About 98% of the radioactivity was ethanol-soluble, and when analyzed by column and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.6.893
更新日期:1969-06-01 00:00:00
abstract::The photosynthetic performance of synchronously grown Chlamydomonas reinhardtii alternated rhythmically during the cell cycle. The activity of the "CO(2) concentrating mechanism" including the ability to accumulate CO(2) internally and the activity of carbonic anhydrase peaked after 6 to 9 hours of light and reached m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.2.604
更新日期:1986-02-01 00:00:00
abstract::Extracellular calmodulin (ExtCaM) exerts multiple functions in animals and plants, but the mode of ExtCaM action is not well understood. In this paper, we provide evidence that ExtCaM stimulates a cascade of intracellular signaling events to regulate stomatal movement. Analysis of the changes of cytosolic free Ca2+ ([...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.047837
更新日期:2004-12-01 00:00:00
abstract::When petunia (Petunia hybrida Vilm, cv Rosy Morn) cells are cultured in the presence of 2 [mu]M antimycin A (AA), respiration proceeds mainly via the cyanide-resistant pathway. Cyanide-resistant respiratory rates were higher in mitochondria from AA cells than in control mitochondria. Compared with control cells, an in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.2.617
更新日期:1997-10-01 00:00:00
abstract::We functionally characterized the grape (Vitis vinifera) VvPIP2;4N (for Plasma membrane Intrinsic Protein) aquaporin gene. Expression of VvPIP2;4N in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that VvPIP2;...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.203455
更新日期:2012-10-01 00:00:00
abstract::The effect of strong irradiance (2000 micromole photons per square meter per second) on PSII heterogeneity in intact cells of Chlamydomonas reinhardtii was investigated. Low light (LL, 15 micromole photons per square meter per second) grown C. reinhardtii are photoinhibited upon exposure to strong irradiance, and the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.4.1196
更新日期:1990-04-01 00:00:00
abstract::Dwarf mutants of pea (Pisum sativum), with impaired gibberellin (GA) biosynthesis in the shoot, were studied to determine whether the roots of these genotypes had altered elongation and GA levels. Mutations na, lh-2, and ls-1 reduced GA levels in root tips and taproot elongation, although in lh-2 and ls-1 roots the re...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.2.627
更新日期:2001-02-01 00:00:00
abstract::The cultivated tomato (Lycopersicon esculentum) has a unipinnate compound leaf. In the developing leaf primordium, major leaflet initiation is basipetal, and lobe formation and early vascular differentiation are acropetal. We show that engineered alterations in the expression of a tomato homeobox gene, LeT6, can cause...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.3.771
更新日期:1998-07-01 00:00:00