A Plant Virus-Based Vector System for Gene Function Studies in Pepper.

Abstract:

:While pepper (Capsicum annuum) is a highly recalcitrant species for genetic transformation studies, plant virus-based vectors can provide alternative and powerful tools for transient regulation and functional analysis of genes of interest in pepper. In this study, we established an effective virus-based vector system applicable for transient gain- and loss-of-function studies in pepper using Broad bean wilt virus2 (BBWV2). We engineered BBWV2 as a dual gene expression vector for simultaneous expression of two recombinant proteins in pepper cells. In addition, we established enhanced and stable expression of recombinant proteins from the BBWV2-based dual vector via coexpression of a heterologous viral suppressor of RNA silencing. We also developed a BBWV2-based virus-induced gene silencing (VIGS) vector, and we successfully silenced the phytoene desaturase gene (PDS) using the BBWV2-based VIGS vector in various pepper cultivars. Additionally, we optimized the BBWV2-based VIGS system in pepper by testing the efficiency of PDS gene silencing under different conditions. This BBWV2-based vector system represents a convenient approach for rapid and simple analysis of gene functions in pepper.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Choi B,Kwon SJ,Kim MH,Choe S,Kwak HR,Kim MK,Jung C,Seo JK

doi

10.1104/pp.19.00836

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

867-880

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.19.00836

journal_volume

181

pub_type

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