Gibberellin biosynthesis mutations and root development in pea.

Abstract:

:Dwarf mutants of pea (Pisum sativum), with impaired gibberellin (GA) biosynthesis in the shoot, were studied to determine whether the roots of these genotypes had altered elongation and GA levels. Mutations na, lh-2, and ls-1 reduced GA levels in root tips and taproot elongation, although in lh-2 and ls-1 roots the reduction in elongation was small (less than 15%). The na mutation reduced taproot length by about 50%. The roots of na plants elongated in response to applied GA(1) and recombining na with mutation sln (which blocks GA catabolism) increased GA(1) levels in root tips and completely restored normal root development. In shoots, Mendel's le-1 mutation impairs the 3beta-hydroxylation of GA(20) to the bioactive GA(1), resulting in dwarfism. However, GA(1) and GA(20) levels were normal in le-1 roots, as was root development. The null mutation le-2 also did not reduce root GA levels or elongation. The results support the theory that GAs are important for normal root elongation in pea, and indicate that a 3beta-hydroxylase gene other than LE operates in pea roots.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Yaxley JR,Ross JJ,Sherriff LJ,Reid JB

doi

10.1104/pp.125.2.627

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

627-33

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

125

pub_type

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