Abstract:
:Recent experiments show that exogenous NADH increases the O(2) consumption and uptake of inorganic ions into isolated corn (Zea mays L. Pioneer Hybrid 3320) root protoplasts (Lin 1982, Proc Natl Acad Sci USA 79: 3773-3776). A mild treatment of protoplasts with trypsin released most of the NADH oxidation system from the plasmalemma (Lin 1982 Plant Physiol 70: 326-328). Further studies on this system showed that exogenous NADH (1.5 millimolar) tripled the proton efflux from the protoplasts thus generating a greater electrochemical proton gradient across the plasmalemma. Trypsin also released ubiquinone (11.95 nanomoles per milligrams protein) but not flavin or cytochrome from the system. Kinetic analyses showed that 1.5 millimolar NADH quadrupled V(max) of the mechanism I (saturable) component of K(+) uptake, while K(m) was not affected. Diethylstibestrol and vanadate inhibited basal (ATPase-mediated) K(+) influx and H(+) efflux, while NADH-stimulated K(+) uptake was not or only slightly inhibited. p-Chloromercuribenzene-sulfonic acid, N,N'-dicyclohexylcarbodiimide, ethidium bromide, and oligomycin inhibited both ATPase- and NADH-mediated H(+) and K(+) fluxes. A combination of 10 millimolar fusicoccin and 1.5 millimolar NADH gave an 11-fold increase of K(+) influx and a more than 3-fold increase of H(+) efflux. It is concluded that a plasmalemma ATPase is not involved in the NADH-mediated ion transport mechanism. NADH oxidase is a -SH containing enzyme (protein) and the proton channel is an important element in this transport system. Fusicoccin synergistically stimulates the effect of NADH on K(+) uptake.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Lin Wdoi
10.1104/pp.74.2.219subject
Has Abstractpub_date
1984-02-01 00:00:00pages
219-22issue
2eissn
0032-0889issn
1532-2548journal_volume
74pub_type
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