Abstract:
:Eukaryotic initiation factor 4A (eIF4A) is a highly conserved RNA-stimulated ATPase and helicase involved in the initiation of messenger RNA translation. Previously, we found that eIF4A interacts with cyclin-dependent kinase A (CDKA), the plant ortholog of mammalian CDK1. Here, we show that this interaction occurs only in proliferating cells where the two proteins coassociate with 5'-cap-binding protein complexes, eIF4F or the plant-specific eIFiso4F. CDKA phosphorylates eIF4A on a conserved threonine residue (threonine-164) within the RNA-binding motif 1b TPGR. In vivo, a phospho-null (APGR) variant of the Arabidopsis (Arabidopsis thaliana) eIF4A1 protein retains the ability to functionally complement a mutant (eif4a1) plant line lacking eIF4A1, whereas a phosphomimetic (EPGR) variant fails to complement. The phospho-null variant (APGR) rescues the slow growth rate of roots and rosettes, together with the ovule-abortion and late-flowering phenotypes. In vitro, wild-type recombinant eIF4A1 and its phospho-null variant both support translation in cell-free wheat germ extracts dependent upon eIF4A, but the phosphomimetic variant does not support translation and also was deficient in ATP hydrolysis and helicase activity. These observations suggest a mechanism whereby CDK phosphorylation has the potential to down-regulate eIF4A activity and thereby affect translation.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Bush MS,Pierrat O,Nibau C,Mikitova V,Zheng T,Corke FM,Vlachonasios K,Mayberry LK,Browning KS,Doonan JHdoi
10.1104/pp.16.00435subject
Has Abstractpub_date
2016-09-01 00:00:00pages
128-40issue
1eissn
0032-0889issn
1532-2548pii
pp.16.00435journal_volume
172pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::An interval on barley (Hordeum vulgare L.) chromosome 7 accounting for significant quantitative trait locus effects for winter hardiness were detected in a winter (Dicktoo) x spring (Morex) barley population (P.M. Hayes, T. Blake, T.H.H. Chen, S. Tragoonrung, F. Chen, A. Pan, and B. Liu [1993] Genome 36: 66-71). Two m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.3.1233
更新日期:1995-07-01 00:00:00
abstract::Evidence that indoleacetic acid (IAA) conjugates are metabolized via enzyme-catalyzed hydrolysis to free IAA and that their biological activities are related to the rates at which they are hydrolyzed by the tissue is presented. These conclusions are based on the following observations. Slow but continuous decarboxylat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.6.1424
更新日期:1981-12-01 00:00:00
abstract::Cotyledons excised from dark-grown cucumber (Cucumis sativus L. cv. Aonagajibai) seedlings were incubated in the dark with the cytokinin benzyladenine for different time periods. Then, various greening parameters were examined, including protochlorophyll(ide) to chlorophyll(ide) photoconversion and delta-aminolevulini...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.3.663
更新日期:1982-03-01 00:00:00
abstract::Five blue-green and one red algal species produced carbon monoxide during photosynthetic growth. The blue-green algae synthesized CO and phycocyanobilin in equimolar quantities at identical rates. The red alga, Porphyridium cruentum, incorporated Delta-aminolevulinic acid-5-(14)C into phycoerythrobilin and CO. The rat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.1.72
更新日期:1973-01-01 00:00:00
abstract::Despite much study of the role of S-adenosylmethionine (SAM) in the methylation of DNA, RNA, and proteins, and as a cofactor for a wide range of biosynthetic processes, little is known concerning the intracellular transport of this essential metabolite. Screening of the Arabidopsis (Arabidopsis thaliana) genome yielde...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086975
更新日期:2006-11-01 00:00:00
abstract::The permeability of the Chlorella pyrenoidosa membrane was studied by following the efflux of (14)C-intracellular material from cells which had been allowed to incorporate (14)CO(2) photosynthetically. It was observed that the efflux increased upon treatment with low concentrations (3-30 muM) of 2, 3-dichloro-1, 4-nap...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.2.363
更新日期:1973-02-01 00:00:00
abstract::Fructose 2,6-bisphosphate inhibits phosphoglucomutase noncompetitively with respect to the cofactor glucose 1,6-bisphosphate. Previous studies from our laboratory had shown that phosphoglucomutase was activated by fructose 2,6-bisphosphate in the absence of added glucose 1,6-bisphosphate. The fructose 2,6-bisphosphate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.980
更新日期:1988-12-01 00:00:00
abstract::The functions of mitochondria during leaf senescence, a type of programmed cell death aimed at the massive retrieval of nutrients from the senescing organ to the rest of the plant, remain elusive. Here, combining experimental and analytical approaches, we showed that mitochondrial integrity in Arabidopsis (Arabidopsis...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01463
更新日期:2016-12-01 00:00:00
abstract::Surface glandular trichomes distributed throughout the aerial parts of sweet basil (Ocimum basilicum) produce and store monoterpene, sesquiterpene, and phenylpropene volatiles. Three distinct basil chemotypes were used to examine the molecular mechanisms underlying the divergence in their monoterpene and sesquiterpene...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.051318
更新日期:2004-11-01 00:00:00
abstract::Aspen (Populus tremuloides Michx.) leaf extracts contain a newly discovered enzyme activity that catalyzes the magnesium ion-dependent elimination of diphosphate from dimethylallyl diphosphate with rearrangement to form isoprene (2-methyl, 1-3-butadiene). This isoprene synthase activity has been partially purified. Th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.4.1588
更新日期:1991-12-01 00:00:00
abstract::The Dof (DNA binding with one finger) transcriptional activator rice (Oryza sativa) prolamin box binding factor (RPBF), which is involved in gene regulation of rice seed storage proteins, has been isolated from rice cDNA expressed sequence tag clones containing the conserved Dof. RPBF is found as a single gene per hap...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082826
更新日期:2006-08-01 00:00:00
abstract::Ochromonas danica Pringsheim, a freshwater chrysomonad, converts d-glucose into l-ascorbic acid over a metabolic pathway that ;inverts' the carbon chain of the sugar. In this respect, l-ascorbic acid formation resembles that found in ascorbic acid-synthesizing animals. It differs from this process in that d-galacturon...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.2.465
更新日期:1982-02-01 00:00:00
abstract::Gas-liquid chromatography-mass spectrometry (GLC-MS) has been used for the separation, detection, and identification of 1,4-benzoxazin-3-ones (hydroxamic acids and lactams) and benzoxazolinones found in maize (Zea mays L.) extracts. Compounds of interest were partitioned into ethyl acetate from aqueous maize seedling ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.1.9
更新日期:1979-01-01 00:00:00
abstract::A large portion of the volatile organic compounds emitted by plants are oxygenated to yield reactive carbonyl species, which have a big impact on atmospheric chemistry. Deposition to vegetation driven by the absorption of reactive carbonyl species into plants plays a major role in cleansing the atmosphere, but the mec...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01045
更新日期:2015-11-01 00:00:00
abstract::We recently identified a cDNA encoding a putative isovaleryl-coenzyme A (CoA) dehydrogenase in Arabidopsis (AtIVD). In animals, this homotetrameric enzyme is located in mitochondria and catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. Ex...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.2.601
更新日期:2001-06-01 00:00:00
abstract::Tomato Atypical Receptor Kinase 1 (TARK1) is a pseudokinase required for postinvasion immunity. TARK1 was originally identified as a target of the Xanthomonas euvesicatoria effector protein Xanthomonas outer protein N (XopN), a suppressor of early defense signaling. How TARK1 participates in immune signal transduction...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01400
更新日期:2020-07-01 00:00:00
abstract::Simultaneous comparisons were made between a freezing-tolerant, cold-acclimating (CA) wild potato species (Solanum commersonii) and a freezing-sensitive, nonacclimating (NA) cultivated species (Solanum tuberosum). Comparative studies allowed differentiation of plasma membrane lipid changes associated with increased fr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.3.793
更新日期:1993-11-01 00:00:00
abstract::The growth of lateral buds (tillers), which are undergoing release from apical dominance, was measured in upright and gravistimulated intact Avena sativa L. cv. ;Victory' (oat) shoots as well as in isolated Avena stem segments treated with kinetin and sucrose. During release, the tiller bud initially shows a slow rate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.811
更新日期:1982-09-01 00:00:00
abstract::Over recent years, a number of initiatives have proposed standard reporting guidelines for functional genomics experiments. Associated with these are data models that may be used as the basis of the design of software tools that store and transmit experiment data in standard formats. Central to the success of such dat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.058875
更新日期:2005-05-01 00:00:00
abstract::Ascorbic acid oxidase activity in Myrothecium verrucaria extracts resulted in O(2) uptake exceeding 0.5 mole per mole of ascorbic acid and in CO(2) evolution. Measurement of oxidized ascorbic acid at completion of the reaction demonstrated that an average of 10% of the oxidized product disappeared. A comparison of the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.10.1553
更新日期:1966-12-01 00:00:00
abstract::Carbonyl sulfide (COS), a substrate for carbonic anhydrase, inhibited alkalization of the medium, O(2) evolution, dissolved inorganic carbon accumulation, and photosynthetic CO(2) fixation at pH 7 or higher by five species of unicellular green algae that had been air-adapted for forming a CO(2)-concentrating process. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.2.578
更新日期:1992-02-01 00:00:00
abstract::Although researchers have established that DNA methylation and active demethylation are dynamically regulated in plant cells, the molecular mechanism for the regulation of active DNA demethylation is not well understood. By using an Arabidopsis (Arabidopsis thaliana) line expressing the Promoter RESPONSIVE TO DEHYDRAT...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.252106
更新日期:2015-03-01 00:00:00
abstract::The basic/helix-loop-helix (bHLH) transcription factors and their homologs form a large family in plant and animal genomes. They are known to play important roles in the specification of tissue types in animals. On the other hand, few plant bHLH proteins have been studied functionally. Recent completion of whole genom...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.080580
更新日期:2006-08-01 00:00:00
abstract::Membrane preparation (sedimenting between 13,000(g) and 80,000(g)) of germinating radish seeds (Raphanus sativus L.) was active in hydrolyzing ATP and, to a lesser extent, a variety of other phosphorylated compounds. Dicyclohexylcarbodiimide (DCCD) and diethylstilbestrol significantly inhibited the ATPase activity (40...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.2.377
更新日期:1981-08-01 00:00:00
abstract::Indole-3-acetic acid (IAA) labeled with (13)C in the six carbons of the benzene ring is described for use as an internal standard for quantitative mass spectral analysis of IAA by gas chromatography/selected ion monitoring. [(13)C(6)]IAA was compared to the available deuterium labeled compounds and shown to offer the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.1.14
更新日期:1986-01-01 00:00:00
abstract::Plants coordinate the expression of photosynthesis-related genes in response to growth and environmental changes. In species that conduct two-cell C4 photosynthesis, expression of photosynthesis genes is partitioned such that leaf mesophyll and bundle sheath cells accumulate different components of the photosynthetic ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01053
更新日期:2020-03-01 00:00:00
abstract::The influence of plasma membrane lipid components on the activity of the H(+)-ATPase has been studied by determining the effect of surfactants on membrane lipids and ATPase activity of oat (Avena sativa L.) root plasma membrane vesicles purified by a two-phase partitioning procedure. Triton X-100, at 25 to 1 (weight/w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.4.1524
更新日期:1989-08-01 00:00:00
abstract::Ribulose 1,5-diphosphate carboxylase (RUDPcase) from autotrophically grown Euglena gracilis was purified to homogeneity as measured by analytical ultracentrifugation, polyacrylamide gel electrophoresis, and immunoprecipitation reactions. The enzyme represented about 9% of total protein and 24% of soluble protein in th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.3.345
更新日期:1975-09-01 00:00:00
abstract::Stimulation of active oxygen metabolism occurs during the early stages of interactions involving bacteria and plant cell suspensions. Although many cellular processes are known to affect active oxygen metabolism in plants, it is not known which of these factors affect active oxygen levels during plant-bacteria interac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.353
更新日期:1995-05-01 00:00:00
abstract::Cell-cell and extracellular cell matrix (ECM) interactions provide cells with information essential for controlling morphogenesis, cell-fate specification, and cell death. In animals, one of the major groups of enzymes that degrade the ECM is the matrix metalloproteinases (MMPs). Here, we report the characterization o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.3.917
更新日期:2000-07-01 00:00:00