Abstract:
:The influence of plasma membrane lipid components on the activity of the H(+)-ATPase has been studied by determining the effect of surfactants on membrane lipids and ATPase activity of oat (Avena sativa L.) root plasma membrane vesicles purified by a two-phase partitioning procedure. Triton X-100, at 25 to 1 (weight/weight) Triton to plasma membrane protein, an amount that causes maximal activation of the ATPase in the ATPase assay, extracted 59% of the membrane protein but did not solubilize the bulk of the ATPase. The Triton-insoluble proteins had associated with them, on a micromole per milligram protein basis, only 14% as much phospholipid, but 38% of the glycolipids and sterols, as compared with the native membranes. The Triton insoluble ATPase could still be activated by Triton X-100. When solubilized by lysolecithin, there were still sterols associated with the ATPase fraction. Free sterols were found associated with the ATPase in the same relative proportions, whether treated with surfactants or not. We suggest that surfactants activate the ATPase by altering the hydrophobic environment around the enzyme. We propose that sterols, through their interaction with the ATPase, may be essential for ATPase activity.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Sandstrom RP,Cleland REdoi
10.1104/pp.90.4.1524subject
Has Abstractpub_date
1989-08-01 00:00:00pages
1524-31issue
4eissn
0032-0889issn
1532-2548journal_volume
90pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::A mutation in the ALTERED XYLOGLUCAN9 (AXY9) gene was found to be causative for the decreased xyloglucan acetylation phenotype of the axy9.1 mutant, which was identified in a forward genetic screen for Arabidopsis (Arabidopsis thaliana) mutants. The axy9.1 mutant also exhibits decreased O-acetylation of xylan, implyin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.256479
更新日期:2015-04-01 00:00:00
abstract::Excised juvenile leaves of Microgramma vacciniifolia (Polypodiaceae) develop sporophytic regenerants when grown on mineral agar with sucrose. The ratio of sporophytes to gametophytes produced from the leaf tissue increases with higher percentages of sucrose such that at 4% sucrose, the induction of aposporous gametoph...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.3.390
更新日期:1975-09-01 00:00:00
abstract::Cyanobacteria unable to fix atmospheric nitrogen have evolved sophisticated adaptations to survive to long periods of nitrogen starvation. These genetic programs are still largely unknown-as evidenced by the many proteins whose expression is regulated in response to nitrogen availability, but which belong to unknown o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00802
更新日期:2020-12-01 00:00:00
abstract::Premature leaf senescence affects plant growth and yield in rice. NAD plays critical roles in cellular redox reactions and remains at a sufficient level in the cell to prevent cell death. Although numerous factors affecting leaf senescence have been identified, few involving NAD biosynthetic pathways have been describ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01898
更新日期:2016-06-01 00:00:00
abstract::The gross tissue distribution, intracellular fate, and chemical behavior of Ni(2+) in soybean plants (Glycine max cv. Williams) were investigated. Following root absorption, Ni was highly mobile in the plant, with leaves being the major sink in the shoots for Ni during vegetative growth. A senescence >70% of the Ni pr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.4.566
更新日期:1978-10-01 00:00:00
abstract::Effects of O(2) and some respiratory inhibitors on the induction and release of bud dormancy were examined with the aerial tubers of different ages of Begonia evansiana Andr. Oxygen was needed not only for tuber sprouting but also during the chilling process at 2 to 5 C to break tuber dormancy. If the mature tubers we...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.3.504
更新日期:1973-03-01 00:00:00
abstract::Electron transport activity at the cell surface of intact oat seedlings (Avena sativa L. cv Garry) was examined by measuring the oxidation and/or reduction of agents in the medium bathing the roots. Oxidation of NADH with or without added electron acceptors and reduction of ferricyanide by an endogenous electron donor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.2.386
更新日期:1984-10-01 00:00:00
abstract::Many race- or isolate-specific disease resistance responses of plants toward pathogens (incompatible interactions) invoke hypersensitive response (HR)-like programmed cell death (PCD) and the coordinated activation of mitogen-activated protein kinases homologous with Arabidopsis (Arabidopsis thaliana) AtMPK6 and AtMPK...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.119511
更新日期:2008-06-01 00:00:00
abstract::This study aims to elucidate the molecular mechanism of an alternative electron flow (AEF) functioning under suppressed (CO2-limited) photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], reaches a maximum shortly...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.249987
更新日期:2015-02-01 00:00:00
abstract::The influence of naturally occurring phenolic acids on phosphate uptake by barley (Hordeum vulgare L. cv. Karlsberg) roots was examined using (32)P-labeled phosphate. Without exception, all compounds tested, namely, benzoic, 2-hydroxybenzoic, 4-hydroxybenzoic, 3,4-dihydroxybenzoic, 3,4,5-trihydroxybenzoic, 4-hydroxy-3...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.6.1037
更新日期:1973-06-01 00:00:00
abstract::The factors that mediate specific responses to the plant hormone ethylene are not fully defined. In particular, it is not known how signaling at the receptor complex can control distinct subsets of ethylene responses. Mutations at the Green-ripe (Gr) and reversion to ethylene sensitivity1 (rte1) loci, which encode hom...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.205476
更新日期:2012-12-01 00:00:00
abstract::Sex determination in cucumber (Cucumis sativus L.) is controlled largely by three genes: F, m, and a. The F and m loci interact to produce monoecious (M_f_) or gynoecious (M_f_) sex phenotypes. Ethylene and factors that induce ethylene biosynthesis, such as 1-aminocyclopropane-1-carboxylate (ACC) and auxin, also enhan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.3.987
更新日期:1997-03-01 00:00:00
abstract::Cotyledon explants from germinated 1-day-old soybean seedling were inoculated with single or mixed strains of Agrobacterium tumefaciens. Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region and a binary vector (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.570
更新日期:1988-11-01 00:00:00
abstract::Root iron mobilization genes are induced by iron deficiency downstream of an unknown signaling mechanism. The FER gene, encoding a basic helix-loop-helix domain protein and putative transcription factor, is required for induction of iron mobilization genes in roots of tomato (Lycopersicon esculentum). To study upstrea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.054270
更新日期:2005-03-01 00:00:00
abstract::Growth of the aerial part of the plant is dependent upon the maintenance of the shoot apical meristem (SAM). A balance between the self-renewing stem cells in the central zone (CZ) and organogenesis in the peripheral zone (PZ) is essential for the integrity, function, and maintenance of the SAM. Understanding how the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.171462
更新日期:2011-06-01 00:00:00
abstract::Maize (Zea mays L.) kernel pedicels, including vascular tissues, pedicel parenchyma, placento-chalazal tissue, and the surrounding pericarp, contained two forms of glutamine synthetase (EC 6.3.1.2), separable by anion exchange chromatography under mildly acidic conditions. The earlier-eluting activity (GS(p1)), but no...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.3.868
更新日期:1989-11-01 00:00:00
abstract::"Active Fe" in plants has generally been interpreted to be ferrous iron. This study evaluated some of the commonly followed active Fe determination procedures based on Fe(II) measurements. In each of 12 species examined, leaf extracts exhibited strong reducing activity and quickly reduced any added Fe(III) with or wit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.3.1017
更新日期:1990-07-01 00:00:00
abstract::Gravity is a critical environmental factor affecting the morphology and functions of organisms on the Earth. Plants sense changes in the gravity vector (gravistimulation) and regulate their growth direction accordingly. In Arabidopsis (Arabidopsis thaliana) seedlings, gravistimulation, achieved by rotating the specime...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.223313
更新日期:2013-10-01 00:00:00
abstract::An alpha-L-fucosidase (EC 3.2.1.51) able to release the t-fucosyl residue from the side chain of xyloglucan oligosaccharides has been detected in the leaves of Arabidopsis plants. Moreover, an alpha-L-fucosidase with similar substrate specificity was purified from cabbage (Brassica oleracea) leaves to render a single ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Photoconvertible fluorescent proteins (FPs) are recent additions to the biologists' toolbox for understanding the living cell. Like green fluorescent protein (GFP), monomeric EosFP is bright green in color but is efficiently photoconverted into a red fluorescent form using a mild violet-blue excitation. Here, we repor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.165431
更新日期:2010-12-01 00:00:00
abstract::Rhizomes are organs of fundamental importance to plant competitiveness and invasiveness. We have identified genes expressed at substantially higher levels in rhizomes than other plant parts, and explored their functional categorization, genomic organization, regulatory motifs, and association with quantitative trait l...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082891
更新日期:2006-11-01 00:00:00
abstract::The Arabidopsis (Arabidopsis thaliana) orthologs of Brca2, a protein whose mutations are involved in breast cancer in humans, were previously shown to be essential at meiosis. In an attempt to better understand the Brca2-interacting properties, we examined four partners of the two isoforms of Brca2 identified in Arabi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.075838
更新日期:2006-03-01 00:00:00
abstract::Reciprocal grafts, and applications of gibberellin (GA) and indoleacetic acid (IAA) were used to localize the site of control for stem elongation in cucumber (Cucumis sativus L.). Dwarf and tall plants were reciprocally grafted to determine influence of stems and roots on stem elongation. At 21 days there were no sign...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.6.942
更新日期:1974-06-01 00:00:00
abstract::The ALCR/alcA (alc) two-component, ethanol-inducible gene expression system provides stringent control of transgene expression in genetically modified plants. ALCR is an ethanol-activated transcription factor that can drive expression from the ALCR-responsive promoter (alcA). However, the alc system has been shown to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.059659
更新日期:2005-07-01 00:00:00
abstract::Winter- and summertime-active desert annual species were grown at different temperatures to assess their capacity for photosynthetic acclimation. Thermal stability of photosynthesis was determined from responses of chlorophyll fluorescence to increased temperature. Photosynthesis in winter ephemerals grown at 28 degre...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.4.786
更新日期:1984-04-01 00:00:00
abstract::Extracts of light-grown Cucumis sativus L. seedlings catalyzed the oxidation of indole-3-acetaldehyde to indole-3-acetic acid. No added cofactors were required. Inhibitor studies indicated that the enzyme is a metalloflavoprotein. While indole-3-aldehyde, benzaldehyde, and phenylacetaldehyde partially inhibited the ox...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.1.107
更新日期:1978-01-01 00:00:00
abstract::We have studied the effects of ATP and ADP on the oxidation of malate by coupled and uncoupled mitochondria prepared from etiolated hypocotyls of mung bean (Vigna radiata L.).In coupled mitochondria, ATP (1 millimolar) increased pyruvate production and decreased oxaloacetate formation without altering the rate of oxyg...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.1.21
更新日期:1984-09-01 00:00:00
abstract::Effects of the herbicide safeners N,N-diallyl-2,2-dichloroacetamide and 4-dichloroacetyl-3,4-dihydro-3-methyl-2H-1,4-benzooxazin (CGA 154281) on the contents in cysteine and glutathione, on the assimilation of (35)SO(4) (2-), and on the enzymes of assimilatory sulfate reduction were analyzed in roots and primary leave...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.4.1808
更新日期:1990-12-01 00:00:00
abstract::Histone H2B monoubiquitination (H2Bub) is being recognized as a regulatory mechanism that controls a range of cellular processes in plants, but the molecular mechanisms of H2Bub that are involved in responses to biotic stress are largely unknown. In this study, we used wild-type and H2Bub loss-of-function mutations of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.234567
更新日期:2014-04-01 00:00:00
abstract::Magnaporthe oryzae is a fungal pathogen that causes rice (Oryza sativa) blast. SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are key components in vesicle trafficking in eukaryotic cells and are known to contribute to fungal pathogen resistance. Syntaxin of Plants121 (SYP121), a Qa-SN...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01013
更新日期:2019-04-01 00:00:00