The effect of sucrose on the differentiation of excised fern leaf tissue into either gametophytes of sporophytes.

Abstract:

:Excised juvenile leaves of Microgramma vacciniifolia (Polypodiaceae) develop sporophytic regenerants when grown on mineral agar with sucrose. The ratio of sporophytes to gametophytes produced from the leaf tissue increases with higher percentages of sucrose such that at 4% sucrose, the induction of aposporous gametophytes is a rare occurrence. Experiments varying the osmotic potential with sorbitol and those holding the osmotic potential of the culture medium constant while varying the sucrose level indicate that the effect of sucrose on the differentiation of fern leaf tissue into either gametophyte or sporophyte is nutritional rather than osmotic. A significant effect of sucrose in altering the differentiation of fern leaf tissue is the increased rate of senescence promoted by high sucrose concentrations.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Hirsch AM

doi

10.1104/pp.56.3.390

subject

Has Abstract

pub_date

1975-09-01 00:00:00

pages

390-3

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

56

pub_type

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