Maintenance of turgor by rapid sealing of puncture wounds in leaf epidermal cells.

Abstract:

:When leaf epidermal cells are puncture wounded with a glass microcapillary tip, a small droplet of fluid is discharged and then evaporates, leaving a solid residue on the cell surface. For puncture wounds of about 3.5 micrometers in diameter, this process is complete within 2 to 3 seconds. A second puncture wound also exhibits a similar discharge, indicating the persistence of some turgor pressure within the cell, despite damage to the cell wall. Direct measurement of turgor on the large epidermal cells of Tradescantia virginiana L. demonstrated that turgor was substantially maintained (91-96%) after puncture wounding. Anatomical and histochemical evidence suggests that the damaged portion of the cell wall was sealed with an amorphous plug of material comprised of pectinaceous polysaccharides. Rapid sealing of puncture wounds and the maintenance of turgor in epidermal cells may be an important functional component of plant adaptation to physical damage such as that caused by insect feeding.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Shackel KA,Polito VS,Ahmadi H

doi

10.1104/pp.97.3.907

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

907-12

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

97

pub_type

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