Abstract:
:We have constructed a chimeric gene consisting of the promoter, first exon, and first intron of a maize ubiquitin gene (Ubi-1) and the coding sequence of the bar gene from Streptomyces hygroscopicus. This construct was transferred into rice (Oryza sativa L.) protoplasts via electroporation, and 10 plants were regenerated from calli that had been selected for resistance to exogenously supplied bialaphos. Transgenic plants grown in a greenhouse were resistant to both bialaphos and phosphinothricine at a dosage lethal to untransformed control plants. Evidence of stable integration of the transferred gene into the genome of the regenerated primary transformant plants was obtained from Southern blot analysis. In addition, northern blot analysis indicated expression and proper splicing of the maize ubiquitin gene first intron from the primary chimeric transcript in these transgenic rice plants, and western blot analysis and enzymic assays verified expression of the active bar gene product. Apparent mendelian segregation for bialaphos resistance in T(1) progeny of primary transformants was confirmed.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Toki S,Takamatsu S,Nojiri C,Ooba S,Anzai H,Iwata M,Christensen AH,Quail PH,Uchimiya Hdoi
10.1104/pp.100.3.1503subject
Has Abstractpub_date
1992-11-01 00:00:00pages
1503-7issue
3eissn
0032-0889issn
1532-2548journal_volume
100pub_type
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