Dark-Induced Senescence Causes Localized Changes in DNA Methylation.

Abstract:

:Senescence occurs in a programmed manner to dismantle the vegetative tissues and redirect nutrients towards metabolic pathways supporting reproductive success. External factors can trigger the senescence program as an adaptive strategy, indicating that this terminal program is controlled at different levels. It has been proposed that epigenetic factors accompany the reprogramming of the senescent genome; however, the mechanism and extent of this reprogramming remain unknown. Using bisulphite conversion followed by sequencing, we assessed changes in the methylome of senescent Arabidopsis (Arabidopsis thaliana) leaves induced by darkness and monitored their effect on gene and transposable element (TE) expression with transcriptome sequencing. Upon dark-induced senescence, genes controlling chromatin silencing were collectively down-regulated. As a consequence, the silencing of TEs was impaired, causing in particular young TEs to become preferentially reactivated. In parallel, heterochromatin at chromocenters was decondensed. Despite the disruption of the chromatin maintenance network, the global DNA methylation landscape remained highly stable, with localized changes mainly restricted to CHH methylation. Together, our data show that the terminal stage of plant life is accompanied by global changes in chromatin structure but only localized changes in DNA methylation, adding another example of the dynamics of DNA methylation during plant development.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Trejo-Arellano MS,Mehdi S,de Jonge J,Dvorák Tomastíková E,Köhler C,Hennig L

doi

10.1104/pp.19.01154

subject

Has Abstract

pub_date

2020-02-01 00:00:00

pages

949-961

issue

2

eissn

0032-0889

issn

1532-2548

pii

pp.19.01154

journal_volume

182

pub_type

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