Enzymes of starch metabolism in the developing rice grain.

Abstract:

:The levels of starch, soluble sugars, protein, and enzymes involved in starch metabolism-alpha-amylase, beta-amylase, phosphorylase, Q-enzyme, R-enzyme, and starch synthetase -were assayed in dehulled developing rice grains (Oryzasativa L., variety IR8). Phosphorylase, Q-enzyme, and R-enzyme had peak activities 10 days after flowering, whereas alpha- and beta-amylases had maximal activities 14 days after flowering. Starch synthetase bound to the starch granule increased in activity up to 21 days after flowering. These enzymes (except the starch synthetases) were also detected by polyacrylamide gel electrophoresis. Their activity in grains at the midmilky stage (8-10 days after flowering) was determined in five pairs of lines with low and high amylose content from different crosses. The samples had similar levels of amylases, phosphorylase, R-enzyme, and Q-enzyme. The samples consistently differed in their levels of starch synthetase bound to the starch granule, which was proportional to amylose content. Granule-bound starch synthetase may be responsible for the integrity of amylose in the developing starch granule.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Baun LC,Palmiano EP,Perez CM,Juliano BO

doi

10.1104/pp.46.3.429

subject

Has Abstract

pub_date

1970-09-01 00:00:00

pages

429-34

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

46

pub_type

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