Abstract:
:Transgenic Flaveria bidentis (a C4 species) plants with an antisense gene directed against the mRNA of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) were used to examine the relationship between the CO2 assimilation rate, Rubisco content, and carbon isotope discrimination. Reduction in the amount of Rubisco in the transgenic plants resulted in reduced CO2 assimilation rates and increased carbon isotope discrimination of leaf dry matter. The H2O exchange was similar in transgenic and wild-type plants, resulting in higher ratios of intercellular to ambient CO2 partial pressures. Carbon isotope discrimination was measured concurrently with CO2 and H2O exchange on leaves of the control plants and T1 progeny with a 40% reduction in Rubisco. From the theory of carbon isotope discrimination in the C4 species, we conclude that the reduction in the Rubisco content in the transgenic plants has led to an increase in bundle-sheath CO2 concentration and CO2 leakage from the bundle sheath; however, some down-regulation of the C4 cycle also occurred.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Von Caemmerer S,Millgate A,Farquhar GD,Furbank RTdoi
10.1104/pp.113.2.469subject
Has Abstractpub_date
1997-02-01 00:00:00pages
469-477issue
2eissn
0032-0889issn
1532-2548pii
113/2/469journal_volume
113pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::We have characterized two related cDNAs (RCI2A and RCI2B) corresponding to genes from Arabidopsis thaliana, the expression of which is transiently induced by low, nonfreezing temperatures. RCI2A and RCI2B encode small (54 amino acids), highly hydrophobic proteins that bear two potential transmembrane domains. They sho...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.2.569
更新日期:1997-10-01 00:00:00
abstract::Three novel beta-xylan xylanohydrolases capable of dissociating ferulated arabinoxylan (Feraxan) from maize (Zea mays L. hybrid B73 x Mo17) coleoptile sections and two conventional beta-xylan xylanohydrolases (xylanases) were purified from a Bacillus subtilis industrial enzyme preparation (Novo Ban L-120). The Feraxan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.4.883
更新日期:1988-08-01 00:00:00
abstract::Threonine dehydratase/deaminase (TD), the first enzyme in the isoleucine biosynthetic pathway, is feedback inhibited by isoleucine. By screening M2 populations of ethyl methane sulfonate-treated Arabidopsis thaliana Columbia wild-type seeds, we isolated five independent mutants that were resistant to L-O-methylthreoni...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.1.43
更新日期:1995-01-01 00:00:00
abstract::Polyamines are small ubiquitous molecules that have been involved in nearly all developmental processes, including the stress response. Nevertheless, no direct evidence of a role of polyamines in the wound response has been described. We have studied the expression of genes involved in polyamine biosynthesis in respon...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.009951
更新日期:2002-11-01 00:00:00
abstract::In plants, photoreceptors transfer light signals to phytochrome-interacting factors (PIFs), inducing the rapid phosphorylation and degradation of PIFs to promote photomorphogenesis. However, the phosphatase responsible for PIF dephosphorylation remains unknown. In this study, we identified a type 1 protein phosphatase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01729
更新日期:2016-03-01 00:00:00
abstract::Evidence for photosynthetic linear electron transport in guard cells was obtained with two sensitive methods of high spacial resolution. Light-dependent diaminobenzidine oxidation (an indicator of PSI) and DCMU-sensitive, light-dependent thiocarbamyl nitroblue tetrazolium reduction (an indicator of PSII) were observed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.2.420
更新日期:1983-02-01 00:00:00
abstract::Leaves of Kalanchoë pinnata were exposed in the dark to air (allowing the fixation of CO(2) into malic acid) or 2% O(2), 0% CO(2) (preventing malic acid accumulation). They were then exposed to bright light in the presence or absence of external CO(2) and light dependent inhibition of photosynthetic properties assesse...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.1.117
更新日期:1988-01-01 00:00:00
abstract::Plant roots rely on inorganic orthophosphate (Pi) transporters to acquire soluble Pi from soil solutions that exists at micromolar levels in natural ecosystems. Here, we functionally characterized a rice (Oryza sativa) Pi transporter, Os Phosphate Transporter-1;3 (OsPHT1;3), that mediates Pi uptake, translocation, and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01097
更新日期:2019-02-01 00:00:00
abstract::We examined cell length, mitosis, and root meristem "cuticle" in different tissues of geostimulated, red light-exposed primary roots of corn (Zea Mays, Wisconsin hybrid 64A x 22R). The examination was done at 15-minute intervals for a period of 240 minutes. Differences in cell elongation between the upper and lower si...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.1.7
更新日期:1978-01-01 00:00:00
abstract::Abscisic acid (ABA) in the stem of akamatsu (Pinus densiflora) was identified and quantified by gas chromatography-mass spectrometry-selected ion monitoring using hexadeuterated ABA as an internal standard. tert-Butyldimethylsilyl ester was used as a derivative of ABA. This derivative had high sensitivity and selectiv...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.525
更新日期:1988-11-01 00:00:00
abstract::ATP sulfurylase and 5'-adenylylsulfate (APS) reductase catalyze two reactions in the sulfate assimilation pathway. Cell fractionation of Arabidopsis leaves revealed that ATP sulfurylase isoenzymes exist in the chloroplast and the cytosol, whereas APS reductase is localized exclusively in chloroplasts. During developme...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.2.715
更新日期:2000-10-01 00:00:00
abstract::Complementary DNA (cDNA) clones encoding calmodulin isoforms were isolated from an Arabidopsis leaf lambdagt10 library by screening with cloned barley calmodulin cDNA probes. Two cDNAs, one a 626-base pair partial-length clone (ACaM-1) and one a 1400-base pair full-length clone (ACaM-2), encode calmodulin polypeptides...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.4.1196
更新日期:1991-08-01 00:00:00
abstract::Branched-chain amino acids (BCAAs) are synthesized in plants from branched-chain keto acids, but their metabolism is not completely understood. The interface of BCAA metabolism lies with branched-chain aminotransferases (BCAT) that catalyze both the last anabolic step and the first catabolic step. In this study, six B...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.154922
更新日期:2010-07-01 00:00:00
abstract::Recently, we isolated dehydroipomeamarone, a new sesquiterpenoid from sweet potato (Ipomoea batatas Lam.) root tissue infected with Ceratocystis fimbriata (Ell. et Halst.). The purpose of this investigation was to determine whether dehydroipomeamarone was a precursor in the biosynthetic pathway of ipomeamarone. The in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.4.649
更新日期:1974-04-01 00:00:00
abstract::Excised juvenile leaves of Microgramma vacciniifolia (Polypodiaceae) develop sporophytic regenerants when grown on mineral agar with sucrose. The ratio of sporophytes to gametophytes produced from the leaf tissue increases with higher percentages of sucrose such that at 4% sucrose, the induction of aposporous gametoph...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.3.390
更新日期:1975-09-01 00:00:00
abstract::Auxin-binding protein 1 subsp. mays (ABP1) has been suggested as a receptor mediating auxin-induced cell expansion and differentiation. In maize (Zea mays), ABP1 is encoded by a single gene, Abp1. The TATA and CAAT promoter elements as well as the transcriptional start site were previously identified and all were foun...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.1.369
更新日期:2000-09-01 00:00:00
abstract::The response of cortical microtubules to low temperature and freezing was assessed for root tips of cold-acclimated and non-acclimated winter rye (Secale cereale L. cv Puma) seedlings using indirect immunofluorescence microscopy with antitubulin antibodies. Roots cooled to 0 or -3 degrees C were fixed for immunofluore...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.1.77
更新日期:1990-05-01 00:00:00
abstract::Two hydroxamic acids isolated from maize extracts, 2,4-dihydroxy-7-methoxy-1,4-(2H)-benzoxazin-3(4H)-one (DIMBOA) and the 2-O-beta-d-glucopyranoside of DIMBOA, inhibit photophosphorylation by spinach chloroplasts. Both cyclic and noncyclic photophosphorylations were inhibited to the same extent. The concentrations pro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.4.941
更新日期:1981-10-01 00:00:00
abstract::Phosphoenolpyruvate carboxylase was purified from corn root tips about 80-fold by centrifugation, ammonium sulfate fractionation, and anion exchange and gel filtration chromatography. The resulting preparation was essentially free from malate dehydrogenase, isocitrate dehydrogenase, malate enzyme, NADH oxidase, and py...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.12.1919
更新日期:1968-12-01 00:00:00
abstract::The succulent leaf of the obligate Crassulacean acid metabolism plant Crassula falcata comprises two distinct types of parenchyma. The peripheral tissue is dark green, whereas the central tissue is relatively colorless. We have investigated whether the conventional interpretation of Crassulacean acid metabolism-simply...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.633
更新日期:1988-11-01 00:00:00
abstract::In order to study the effects of desiccation on a photosynthetic system, light harvesting and light-induced electron transport processes were examined in pea cotyledons at various moisture levels, using in vivo fluorescence excitation spectra and fluorescence induction kinetics. Water sorption isotherms yielded thermo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.1.248
更新日期:1985-09-01 00:00:00
abstract::We have previously reported the use of a combination of computational simulations and targeted experiments to build a first generation mathematical model of peppermint (Menthaxpiperita) essential oil biosynthesis. Here, we report on the expansion of this approach to identify the key factors controlling monoterpenoid e...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.152256
更新日期:2010-04-01 00:00:00
abstract::Excision of spinach (Spinacia oleracea L.) leaves had no effect on photosynthetic rates, but altered normal carbon partitioning to favor increased formation of starch and decreased formation of sucrose. The changes were evident within 2 hours after excision. Concurrently, leaf fructose-2,6-bisphosphate content increas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.2.445
更新日期:1984-02-01 00:00:00
abstract::In the last few years, much attention has been given to the role of proteins that accumulate during water deficit. In this work, we analyzed the electrophoretic patterns of basic protein extracts, enriched for a number of cell-wall proteins, from bean (Phaseolus vulgaris L.) seedlings and 21-d-old plants subjected to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.4.1119
更新日期:1995-04-01 00:00:00
abstract::The aim of this work was to investigate starch granule numbers in Arabidopsis (Arabidopsis thaliana) leaves. Lack of quantitative information on the extent of genetic, temporal, developmental, and environmental variation in granule numbers is an important limitation in understanding control of starch degradation and t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.186957
更新日期:2012-02-01 00:00:00
abstract::A physiologically significant level of intracellular carbonic anhydrase has been identified in Chlamydomonas reinhardtii after lysis of the cell wall-less mutant, cw15, and two intracellular polypeptides have been identified which bind to anti-carbonic anhydrase antisera. The susceptibility of the intracellular activi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.3.904
更新日期:1989-03-01 00:00:00
abstract::Nicotianamine (NA) occurs in all plants and chelates metal cations, including FeII, but reportedly not FeIII. However, a comparison of the FeII and ZnII affinity constants of NA and various FeIII-chelating aminocarboxylates suggested that NA should chelate FeIII. High-voltage electrophoresis of the FeNA complex formed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.3.1107
更新日期:1999-03-01 00:00:00
abstract::The appearance and disappearance of NADH:nitrate reductase (NR) in the leaves of corn (Zea mays L. W64A x W182E) were studied using activity assays, an enzyme-linked immunosorbent assay (ELISA) and western blotting. N-starved, etiolated corn plants were treated with nutrients containing either 35 millimolar NH(4)-nitr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.2.442
更新日期:1986-02-01 00:00:00
abstract::Aspen (Populus tremuloides Michx.) leaf extracts contain a newly discovered enzyme activity that catalyzes the magnesium ion-dependent elimination of diphosphate from dimethylallyl diphosphate with rearrangement to form isoprene (2-methyl, 1-3-butadiene). This isoprene synthase activity has been partially purified. Th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.4.1588
更新日期:1991-12-01 00:00:00
abstract::Eukaryotic elongation factor 1alpha (eEF1A) can be post-translationally modified by the addition of phosphorylglycerylethanolamine (PGE). [(14)C]Ethanolamine was incorporated into the PGE modification, and with carrot (Daucus carota L.) suspension culture cells, eEF1A was the only protein that incorporated detectable ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.3.957
更新日期:2000-03-01 00:00:00