OsPHT1;3 Mediates Uptake, Translocation, and Remobilization of Phosphate under Extremely Low Phosphate Regimes.


:Plant roots rely on inorganic orthophosphate (Pi) transporters to acquire soluble Pi from soil solutions that exists at micromolar levels in natural ecosystems. Here, we functionally characterized a rice (Oryza sativa) Pi transporter, Os Phosphate Transporter-1;3 (OsPHT1;3), that mediates Pi uptake, translocation, and remobilization. OsPHT1;3 was directly regulated by Os Phosphate Starvation Response-2 and, in response to Pi starvation, showed enhanced expression in young leaf blades and shoot basal regions and even more so in roots and old leaf blades. OsPHT1;3 was able to complement a yeast mutant strain defective in five Pi transporters and mediate Pi influx in Xenopus laevis oocytes. Overexpression of OsPHT1;3 led to increased Pi concentration both in roots and shoots. However, unlike that reported for other known OsPHT1 members that facilitate Pi uptake at relatively higher Pi levels, mutation of OsPHT1;3 impaired Pi uptake and root-to-shoot Pi translocation only when external Pi concentration was below 5 μm Moreover, in basal nodes, the expression of OsPHT1;3 was restricted to the phloem of regular vascular bundles and enlarged vascular bundles. An isotope labeling experiment with 32P showed that ospht1;3 mutant lines were impaired in remobilization of Pi from source to sink leaves. Furthermore, overexpression and mutation of OsPHT1;3 led to reciprocal alteration in the expression of OsPHT1;2 and several other OsPHT1 genes. Yeast-two-hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays all demonstrated a physical interaction between OsPHT1;3 and OsPHT1;2. Taken together, our results indicate that OsPHT1;3 acts as a crucial factor for Pi acquisition, root-to-shoot Pi translocation, and redistribution of phosphorus in plants growing in environments with extremely low Pi levels.


Plant Physiol


Plant physiology


Chang MX,Gu M,Xia YW,Dai XL,Dai CR,Zhang J,Wang SC,Qu HY,Yamaji N,Feng Ma J,Xu GH




Has Abstract


2019-02-01 00:00:00














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  • Endogenous Gibberellins from Sporophytes of Two Tree Ferns, Cibotium glaucum and Dicksonia antarctica.

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    authors: Yamane H,Fujioka S,Spray CR,Phinney BO,Macmillan J,Gaskin P,Takahashi N

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    authors: Cho MJ,Harper JE

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    authors: Ackerson RC

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  • Growth, Water Relations, and Accumulation of Organic and Inorganic Solutes in Roots of Maize Seedlings during Salt Stress.

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    authors: Rodriguez HG,Roberts J,Jordan WR,Drew MC

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    authors: Katavic V,Reed DW,Taylor DC,Giblin EM,Barton DL,Zou J,Mackenzie SL,Covello PS,Kunst L

    更新日期:1995-05-01 00:00:00

  • Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature.

    abstract::The identity and scope of chloroplast and mitochondrial proteases in higher plants has only started to become apparent in recent years. Biochemical and molecular studies suggested the existence of Clp, FtsH, and DegP proteases in chloroplasts, and a Lon protease in mitochondria, although currently the full extent of t...

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    更新日期:2001-04-01 00:00:00

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    authors: Michael W,Schultz A,Meshcheryakov AB,Ehwald R

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    authors: Fukaki H,Fujisawa H,Tasaka M

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    abstract::Under normal field growth conditions, canola (Brassica napus) seeds produce chloroplasts during early seed development and then catabolize the photosynthetic machinery during seed maturation, producing mature seeds at harvest that are essentially free of chlorophyll (Chl). However, frost exposure early in canola seed ...

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