OsPHT1;3 Mediates Uptake, Translocation, and Remobilization of Phosphate under Extremely Low Phosphate Regimes.


:Plant roots rely on inorganic orthophosphate (Pi) transporters to acquire soluble Pi from soil solutions that exists at micromolar levels in natural ecosystems. Here, we functionally characterized a rice (Oryza sativa) Pi transporter, Os Phosphate Transporter-1;3 (OsPHT1;3), that mediates Pi uptake, translocation, and remobilization. OsPHT1;3 was directly regulated by Os Phosphate Starvation Response-2 and, in response to Pi starvation, showed enhanced expression in young leaf blades and shoot basal regions and even more so in roots and old leaf blades. OsPHT1;3 was able to complement a yeast mutant strain defective in five Pi transporters and mediate Pi influx in Xenopus laevis oocytes. Overexpression of OsPHT1;3 led to increased Pi concentration both in roots and shoots. However, unlike that reported for other known OsPHT1 members that facilitate Pi uptake at relatively higher Pi levels, mutation of OsPHT1;3 impaired Pi uptake and root-to-shoot Pi translocation only when external Pi concentration was below 5 μm Moreover, in basal nodes, the expression of OsPHT1;3 was restricted to the phloem of regular vascular bundles and enlarged vascular bundles. An isotope labeling experiment with 32P showed that ospht1;3 mutant lines were impaired in remobilization of Pi from source to sink leaves. Furthermore, overexpression and mutation of OsPHT1;3 led to reciprocal alteration in the expression of OsPHT1;2 and several other OsPHT1 genes. Yeast-two-hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays all demonstrated a physical interaction between OsPHT1;3 and OsPHT1;2. Taken together, our results indicate that OsPHT1;3 acts as a crucial factor for Pi acquisition, root-to-shoot Pi translocation, and redistribution of phosphorus in plants growing in environments with extremely low Pi levels.


Plant Physiol


Plant physiology


Chang MX,Gu M,Xia YW,Dai XL,Dai CR,Zhang J,Wang SC,Qu HY,Yamaji N,Feng Ma J,Xu GH




Has Abstract


2019-02-01 00:00:00














  • Light-Stimulated Carotenoid Biosynthesis during Transformation of Maize Etioplasts Is Regulated by Increased Activity of Isopentenyl Pyrophosphate Isomerase.

    abstract::Light-stimulated carotenoid biosynthesis associated with the transformation of etioplasts to chloroplasts was investigated after dark-grown maize (Zea mays) seedlings were transferred into light. These studies focused on the enzymes of the pathway to detect those enzyme activities that were stimulated in the light and...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Albrecht M,Sandmann G

    更新日期:1994-06-01 00:00:00

  • High genetic variability of herbivore-induced volatile emission within a broad range of maize inbred lines.

    abstract::Maize plants (Zea mays) attacked by caterpillars release a mixture of odorous compounds that attract parasitic wasps, natural enemies of the herbivores. We assessed the genetic variability of these induced volatile emissions among 31 maize inbred lines representing a broad range of genetic diversity used by breeders i...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Degen T,Dillmann C,Marion-Poll F,Turlings TC

    更新日期:2004-08-01 00:00:00

  • The Metabolism of Oat Leaves during Senescence: II. Senescence in Leaves Attached to the Plant.

    abstract::The course of senescence in the first leaves of light-grown Avena seedlings when attached to the plant has been compared with that previously studied in detached leaves and leaf segments. Proteolysis in the leaf, whether attached or detached, is accompanied by markedly polar basipetal transport of amino acids. This po...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Thimann KV,Tetley RR,Van Thanh T

    更新日期:1974-12-01 00:00:00

  • Potassium and sodium absorption kinetics in roots of two tomato species : lycopersicon esculentum and lycopersicon cheesmanii.

    abstract::Excised roots of the tomato species, Lycopersicon esculentum Mill. cv Walter (the commercial species) and of Lycopersicon cheesmanii ssp. minor (Hook.) C.H. Mull. (a wild species from the Galapagos Islands), were used in comparative studies of their absorption of K(+) and Na(+). Uptake of (86)Rb-labeled K(+) and (22)N...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wrona AF,Epstein E

    更新日期:1985-12-01 00:00:00

  • Sodium Requirement for Photosynthesis and Its Relationship with Dinitrogen Fixation and the External CO(2) Concentration in Cyanobacteria.

    abstract::Cells of Anabaena PCC 7119 and of a mutant strain of Nostoc muscorum unable to fix dinitrogen, grown at pH 8 and under low CO(2) tension (air), showed a reduced capacity for photosynthesis when cultured in the absence of sodium, this inhibition being followed by symptoms of photooxidation, such as chlorosis, oxygen co...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Maeso ES,Piñas FF,Gonzalez MG,Valiente EF

    更新日期:1987-10-01 00:00:00

  • Far-Red Light Reflection from Green Leaves and Effects on Phytochrome-Mediated Assimilate Partitioning under Field Conditions.

    abstract::The influence of plant spacing and row orientation on spectral distribution of light received by growing soybean (Gylcine max [L.] Merr.) plants was measured under field conditions. Light absorption, reflection and transmission of individual leaves showed that most of the blue and red was absorbed while most of the fa...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Kasperbauer MJ

    更新日期:1987-10-01 00:00:00

  • Carbon isotope discrimination varies genetically in c(4) species.

    abstract::Carbon-isotope discrimination (Delta) is used to distinguish between different photosynthetic pathways. It has also been shown that variation in Delta occurs among varieties of C(3) species, but not as yet, in C(4) species. We now report that Delta also varies among genotypes of sorghum (Sorghum bicolor Moench), a C(4...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hubick KT,Hammer GL,Farquhar GD,Wade LJ,von Caemmerer S,Henderson SA

    更新日期:1990-02-01 00:00:00

  • Responses of Rj(1) and rj(1) Soybean Isolines to Inoculation with Bradyrhizobium japonicum.

    abstract::We evaluated the symbiotic phenotypes of nodulation-restrictive and normal soybean isolines by inoculating Clark (genotypically Rj(1)Rj(1)) and mutant Clark-rj(1) (genotypically rj(1)rj(1)) seedlings in plastic growth pouches. Nodules first appeared on Clark seedlings inoculated with Bradyrhizobium japonicum USDA 94 a...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Pueppke SG,Payne JH

    更新日期:1987-08-01 00:00:00

  • Sucrose Synthase Expression during Cold Acclimation in Wheat.

    abstract::When wheat (Triticum aestivum) seedlings are exposed to a cold temperature (2-4 degrees C) above 0 degrees C, sucrose accumulates and sucrose synthase activity increases. The effect of a cold period on the level of sucrose synthase (SS) was investigated. Using antibodies against wheat germ SS, Western blots studies sh...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Crespi MD,Zabaleta EJ,Pontis HG,Salerno GL

    更新日期:1991-07-01 00:00:00

  • Changes in the Physical State of Membrane Lipids during Senescence of Rose Petals.

    abstract::Changes in the physical state of microsomal membrane lipids during senescence of rose flower petals (Rosa hyb. L. cv Mercedes) were measured by x-ray diffraction analysis. During senescence of cut flowers held at 22 degrees C, lipid in the ordered, gel phase appeared in the otherwise disordered, liquid-crystalline pha...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Faragher JD,Wachtel E,Mayak S

    更新日期:1987-04-01 00:00:00

  • Water relationships and incorporation of C assimilates in tubers of potato plants differing in potassium nutrition.

    abstract::Potato (Solanum tuberosum L. cv Saturna) plants were grown in pots with varying supplies of K fertilizer (1.25, 5, and 10 grams K(2)O per 12 kilograms soil). Four weeks after midflower, plants were supplied with (14)CO(2) for 12 hours and osmotic and water potential in tubers were determined. Assimilation of (14)CO(2)...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Beringer H,Haeder HE,Lindhauer M

    更新日期:1983-12-01 00:00:00

  • A Seed Storage Protein with Possible Self-Affinity through Lectin-Like Binding.

    abstract::The primary storage protein of oat (Avena sativa L.) seeds, globulin, was shown to have a specific carbohydrate-binding activity. The globulin was capable of hemagglutinating rabbit red blood cells and this hemagglutination was inhibited by the beta-glucan, laminarin, as well as by carbohydrate which had been cleaved ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Langston-Unkefer PJ,Gade W

    更新日期:1984-03-01 00:00:00

  • AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis.

    abstract::In Arabidopsis (Arabidopsis thaliana), the Shaker K(+) channel AKT1 conducts K(+) uptake in root cells, and its activity is regulated by CBL1/9-CIPK23 complexes as well as by the AtKC1 channel subunit. CIPK23 and AtKC1 are both involved in the AKT1-mediated low-K(+) (LK) response; however, the relationship between the...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wang XP,Chen LM,Liu WX,Shen LK,Wang FL,Zhou Y,Zhang Z,Wu WH,Wang Y

    更新日期:2016-04-01 00:00:00

  • Storage and maintaining activity of ribulose bisphosphate carboxylase/oxygenase.

    abstract::Purified ribulose-1,5-bisphosphate carboxylase/oxygenase in 50% saturated (NH(4))(2)SO(4) was stable when frozen as small beads in liquid nitrogen and stored at -80 C. When stored as a slurry at 4 C most of the activity was lost within four weeks. This loss was due not only to enzyme polymerization. Activity in old pr...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hall NP,McCurry SD,Tolbert NE

    更新日期:1981-06-01 00:00:00

  • Ascorbate biosynthesis in mitochondria is linked to the electron transport chain between complexes III and IV.

    abstract::Ascorbic acid is synthesized from galactono-gamma-lactone (GL) in plant tissues. An improved extraction procedure involving ammonium sulfate precipitation of membrane proteins from crude leaf homogenates yielded a simple, quick method for determining tissue activities of galactono-gamma-lactone dehydrogenase (GLDH). T...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bartoli CG,Pastori GM,Foyer CH

    更新日期:2000-05-01 00:00:00

  • Regulation of Corn Leaf Nitrate Reductase : II. Synthesis and Turnover of the Enzyme's Activity and Protein.

    abstract::The appearance and disappearance of NADH:nitrate reductase (NR) in the leaves of corn (Zea mays L. W64A x W182E) were studied using activity assays, an enzyme-linked immunosorbent assay (ELISA) and western blotting. N-starved, etiolated corn plants were treated with nutrients containing either 35 millimolar NH(4)-nitr...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Remmler JL,Campbell WH

    更新日期:1986-02-01 00:00:00

  • Proton Transport Activity of the Purified Vacuolar H-ATPase from Oats : Direct Stimulation by Cl.

    abstract::To determine whether the detergent-solubilized and purified vacuolar H(+)-ATPase from plants was active in H(+) transport, we reconstituted the purified vacuolar ATPase from oat roots (Avena sativa var Lang). Triton-solubilized ATPase activity was purified by gel filtration and ion exchange chromatography. Incorporati...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ward JM,Sze H

    更新日期:1992-07-01 00:00:00

  • Extracellular Matrix Assembly in Diatoms (Bacillariophyceae) (I. A Model of Adhesives Based on Chemical Characterization and Localization of Polysaccharides from the Marine Diatom Achnanthes longipes and Other Diatoms).

    abstract::Extracellular adhesives from the diatoms Achnanthes longipes, Amphora coffeaeformis, Cymbella cistula, and Cymbella mexicana were characterized by monosaccharide and methylation analysis, lectin-fluorescein isothiocyanate localization, and cytochemical staining. Polysaccharide was the major component of adhesives form...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wustman BA,Gretz MR,Hoagland KD

    更新日期:1997-04-01 00:00:00

  • Short-term leaf elongation kinetics of maize in response to salinity are independent of the root.

    abstract::The essentiality of roots to the short-term responses of leaf elongation to salinity was tested by removing the roots of maize (Zea mays L.) from the shoots and comparing the initial short-term response of leaf elongation to that with intact plants. Eightday-old seedlings growing in solution culture were treated with ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cramer GR,Bowman DC

    更新日期:1991-03-01 00:00:00

  • A New UV-A/B Protecting Pigment in the Terrestrial Cyanobacterium Nostoc commune.

    abstract::A new ultraviolet (UV)-A/B absorbing pigment with maxima at 312 and 330 nanometers from the cosmopolitan terrestrial cyanobacterium Nostoc commune is described. The pigment is found in high amounts (up to 10% of dry weight) in colonies grown under solar UV radiation but only in low concentrations in laboratory culture...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Scherer S,Chen TW,Böger P

    更新日期:1988-12-01 00:00:00

  • Effects of changing stomatal width in a red pine forest on soil water content, leaf water potential, bole diameter, and growth.

    abstract::Spraying a 16 meter tall stand of red pine (Pinus resinosa Ait.) with 10(-3)m phenylmercuric acetate in early June and again in mid-July resulted in the water use between June 1 and October 25 being reduced by almost 10%. It was demonstrated that this was caused by an increase in the leaf resistance with partial stoma...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Turner NC,Waggoner PE

    更新日期:1968-06-01 00:00:00

  • Light and CO(2) Response of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activation in Arabidopsis Leaves.

    abstract::The requirements for activation of ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) were investigated in leaves of Arabidopsis wild-type and a mutant incapable of light activating rubisco in vivo. Upon illumination with saturating light intensities, the activation state of rubisco increased 2-fold in the wild...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Salvucci ME,Portis AR,Ogren WL

    更新日期:1986-03-01 00:00:00

  • circRNAs Are Involved in the Rice-Magnaporthe oryzae Interaction.

    abstract::Circular RNAs (circRNAs) play roles in various biological processes, but their functions in the rice (Oryza sativa) response to Magnaporthe oryzae remain elusive. Here, we demonstrate that circRNAs are involved in the rice-M. oryzae interaction using comparative circRNA-sequencing and transgenic approaches. We identif...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fan J,Quan W,Li GB,Hu XH,Wang Q,Wang H,Li XP,Luo X,Feng Q,Hu ZJ,Feng H,Pu M,Zhao JQ,Huang YY,Li Y,Zhang Y,Wang WM

    更新日期:2020-01-01 00:00:00

  • Floral stimulus movement in perilla and flower inhibition caused by noninduced leaves.

    abstract::Photoperiodic control of flowering in the short day plant Perilla involves the transmission of a floral stimulus from induced leaves to the shoot apex. We have studied the basipetal movement of this stimulus and of (14)C-labeled assimilates in plants with an induced leaf (donor) grafted into the uppermost internode of...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: King RW,Zeevaart JA

    更新日期:1973-04-01 00:00:00

  • Effects of Air Contact on Growth, Inorganic Carbon Sources, and Nitrogen Uptake by an Amphibious Freshwater Macrophyte.

    abstract::Callitriche cophocarpa Sendtner is a heterophyllous amphibious macrophyte that produces apical rosettes of floating leaves. The importance of air contact for inorganic carbon and N uptake and for growth was investigated. Plants were grown with the floating rosette in contact with air of various humidities (10, 50, and...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Madsen TV,Breinholt M

    更新日期:1995-01-01 00:00:00

  • Inactivation of maize phosphoenolpyruvate carboxylase by urea.

    abstract::Phosphoenolpyruvate carboxylase purified from leaves of maize (Zea mays, L.) is sensitive to the presence of urea. Exposure to 2.5 m urea for 30 min completely inactivates the enzyme, whereas for a concentration of 1.5 m urea, about 1 h is required. Malate appears to have no effect on inactivation by urea of phosphoen...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wedding RT,Dole P,Chardot TP,Wu MX

    更新日期:1992-11-01 00:00:00

  • Changes in Mitochondrial Respiratory Chain Components of Petunia Cells during Culture in the Presence of Antimycin A.

    abstract::When petunia (Petunia hybrida Vilm, cv Rosy Morn) cells are cultured in the presence of 2 [mu]M antimycin A (AA), respiration proceeds mainly via the cyanide-resistant pathway. Cyanide-resistant respiratory rates were higher in mitochondria from AA cells than in control mitochondria. Compared with control cells, an in...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wagner AM,Wagner MJ

    更新日期:1997-10-01 00:00:00

  • Kinetic Variance of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Isolated from Diverse Taxonomic Sources : II. Analysis by Two Dual Label Methods.

    abstract::Two dual label methods were used to investigate kinetic variability of ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (EC In addition to using [1-(14)C,5-(3)H]RuBP (method 1), we describe here the detailed assay with (14)CO(2) and [5-(3)H]RuBP (method 2), which generates [(3)H,(14)C]3-phosphoglyceri...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Kent SS,Tomany MJ

    更新日期:1984-07-01 00:00:00

  • Calcium Levels Affect the Ability to Immunolocalize Calmodulin to Cortical Microtubules.

    abstract::Calcium affects the stability of cortical microtubules (MTs) in lysed protoplasts. This calmodulin (CaM)-mediated interaction may provide a mechanism that serves to integrate cellular behavior with MT function. To test the hypothesis that CaM associates with these MTs, monoclonal antibodies were produced against CaM, ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fisher DD,Cyr RJ

    更新日期:1993-10-01 00:00:00

  • Ovule abortion in Arabidopsis triggered by stress.

    abstract::Environmental stresses frequently decrease plant fertility. In Arabidopsis, the effect of salt stress on reproduction was examined using plants grown in hydroponic medium. Salt stress inhibited microsporogenesis and stamen filament elongation. Because plants grown in hydroponic media can be rapidly and transiently str...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Sun K,Hunt K,Hauser BA

    更新日期:2004-08-01 00:00:00