Pyruvate dehydrogenase complex from germinating castor bean endosperm.

Abstract:

:Subcellular organelles from castor bean (Ricinus communis) endosperm were isolated on discontinuous sucrose gradients from germinating seeds which were 1 to 7 days postimbibition. Marker enzyme activities of the organelles were measured (fumarase, catalase, and triose phosphate isomerase) and the homogeneity of the organelle fractions was examined by electron microscopy. Pyruvate dehydrogenase complex activity was measured only in the mitochondrial fraction and attempts to activate or release the enzyme from the proplastid were not successful. A pathway is proposed for the most efficient use of endosperm carbon for de novo fatty acid biosynthesis that does not require the presence of the pyruvate dehydrogenase complex in the proplastid to provide acetyl-coenzymeA.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Rapp BJ,Randall DD

doi

10.1104/pp.65.2.314

subject

Has Abstract

pub_date

1980-02-01 00:00:00

pages

314-8

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

65

pub_type

杂志文章
  • The Transcription Factor MYB29 Is a Regulator of ALTERNATIVE OXIDASE1a.

    abstract::Plants sense and integrate a variety of signals from the environment through different interacting signal transduction pathways that involve hormones and signaling molecules. Using ALTERNATIVE OXIDASE1a (AOX1a) gene expression as a model system of retrograde or stress signaling between mitochondria and the nucleus, MY...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01494

    authors: Zhang X,Ivanova A,Vandepoele K,Radomiljac J,Van de Velde J,Berkowitz O,Willems P,Xu Y,Ng S,Van Aken O,Duncan O,Zhang B,Storme V,Chan KX,Vaneechoutte D,Pogson BJ,Van Breusegem F,Whelan J,De Clercq I

    更新日期:2017-03-01 00:00:00

  • Possible involvement of calmodulin and the cytoskeleton in electrofusion of plant protoplasts.

    abstract::Calmodulin antagonists, trifluoperazine, chlorpromazine, calmidazolium, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), strongly inhibited the electrofusion of barley (Hordeum vulgare L. cv Moor) protoplasts with a marked increase of broken fusion products, after 60 minutes of incubation. W-5, a dechlorinate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.4.1151

    authors: Abe S,Takeda J

    更新日期:1986-08-01 00:00:00

  • Alterations in Chloroplast Thylakoids during an in Vitro Freeze-Thaw Cycle.

    abstract::Plastocyanin and chloroplast coupling factor 1 (CF(1)) are released from spinach (Spinacia oleracea L.) thylakoids during a slow freezethaw cycle. CF(1) addition increases the proton uptake of thylakoids previously frozen in sucrose concentrations of 15 mm to 100 mm. Addition of CF(1) and plastocyanin restores the pro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.5.673

    authors: Garber MP,Steponkus PL

    更新日期:1976-05-01 00:00:00

  • Role of asparagine in the photorespiratory nitrogen metabolism of pea leaves.

    abstract::In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.2.334

    authors: Ta TC,Joy KW,Ireland RJ

    更新日期:1985-06-01 00:00:00

  • The reb1-1 mutation of Arabidopsis. Effect on the structure and localization of galactose-containing cell wall polysaccharides.

    abstract::The Arabidopsis (Arabidopsis thaliana) root epidermal bulger1-1 (reb1-1) mutant (allelic to root hair defective1 [rhd1]) is characterized by a reduced root elongation rate and by bulging of trichoblast cells. The REB1/RHD1 gene belongs to a family of UDP-D-Glucose 4-epimerases involved in the synthesis of D-Galactose ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.074997

    authors: Nguema-Ona E,Andème-Onzighi C,Aboughe-Angone S,Bardor M,Ishii T,Lerouge P,Driouich A

    更新日期:2006-04-01 00:00:00

  • Effect of Potassium Deficiency upon Translocation of C in Detached Blades of Sugarcane.

    abstract::The translocation of radioactive photosynthate was studied in blades detached from plants which had been grown in nutrient solution with and without potassium. Basipetal translocation decreased in the blades of plants deprived of potassium, even when deficiency symptoms were not visible. In such slightly potassium-def...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.2.183

    authors: Hartt CE

    更新日期:1970-02-01 00:00:00

  • Uptake and Utilization of Xylem-borne Amino Compounds by Shoot Organs of a Legume.

    abstract::Amino compounds representative of the major N solutes of xylem sap were pulse-fed (10 to 20 minutes) singly in (14)C-labeled form to cut transpiring shoots of white lupin (Lupinus albus L.). (14)C distribution was studied by autoradiography and radioassays of phloem sap, leaflet tissues, and shoot parts harvested at i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.6.1076

    authors: McNeil DL,Atkins CA,Pate JS

    更新日期:1979-06-01 00:00:00

  • A Seed Storage Protein with Possible Self-Affinity through Lectin-Like Binding.

    abstract::The primary storage protein of oat (Avena sativa L.) seeds, globulin, was shown to have a specific carbohydrate-binding activity. The globulin was capable of hemagglutinating rabbit red blood cells and this hemagglutination was inhibited by the beta-glucan, laminarin, as well as by carbohydrate which had been cleaved ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.3.675

    authors: Langston-Unkefer PJ,Gade W

    更新日期:1984-03-01 00:00:00

  • Induction of curvature in maize roots by calcium or by thigmostimulation: role of the postmitotic isodiametric growth zone.

    abstract::We examined the response of primary roots of maize (Zea mays L. cv Merit) to unilateral application of calcium with particular attention to the site of application, the dependence on growth rate, and possible contributions of thigmotropic stimulation during application. Unilateral application of agar to the root cap ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.762

    authors: Ishikawa H,Evans ML

    更新日期:1992-01-01 00:00:00

  • Changes in the Enzymes for Fatty Acid Synthesis and Desaturation during Acclimation of Developing Soybean Seeds to Altered Growth Temperature.

    abstract::Temperature-induced changes in the enzymes for fatty acid synthesis and desaturation were studied in developing soybean seeds (Glycine max L. var Williams 82). Changes were induced by culture of the seed pods for 20 hours in liquid media at 20, 25, or 35 degrees C. Linoleoyl and oleoyl desaturases were 94 and 10 times...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.2.760

    authors: Cheesbrough TM

    更新日期:1989-06-01 00:00:00

  • The effect soaking pea seeds with or without seedcoats has on seedling growth.

    abstract::Pea seeds (Pisum sativum L. ;Alaska') with intact seedcoats (WC) and with seedcoats removed (WOC) were soaked in distilled water for 24 hours at 20 degrees . The water, containing the pea diffusate, was decanted after the second, fourth, sixth, eighth, twelfth, and twenty-fourth hour and analyzed for total nitrogen, a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.2.255

    authors: Larson LA

    更新日期:1968-02-01 00:00:00

  • The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy.

    abstract::Seed dormancy is a common phase of the plant life cycle, and several parts of the seed can contribute to dormancy. Whole seeds, seeds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) were used in experiments designed to identify components of the Arabidopsis seed that cont...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.093435

    authors: Bethke PC,Libourel IG,Aoyama N,Chung YY,Still DW,Jones RL

    更新日期:2007-03-01 00:00:00

  • Growth Response of Halophila engelmanni Aschers. to Sulfide, Copper, and Organic Nitrogen in Marine Sediments.

    abstract::Growth response of the seagrass Halophila engelmanni Aschers. was studied using defined culture techniques. When added to the rhizosphere, nutrient-seawater medium supported vigorous plant growth, if substantial Cu (10 times that in modified Hoagland solution) plus organic N were present. H(2)S up to 0.2 millimolar in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.4.975

    authors: Pulich WM

    更新日期:1983-04-01 00:00:00

  • Expression of the Large ATP Synthase Gene Cluster in Spinach Plastids during Light-Induced Development.

    abstract::The large ATP synthase gene cluster in spinach (Spinacia oleracea) plastids encodes four of the six chloroplast-encoded ATP synthase subunits. Expression of this cluster was examined to determine its response to light-induced plastid development. Spinach plastid transcripts were isolated from etiolated tissues, etiola...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.3.1164

    authors: Green CD,Hollingsworth MJ

    更新日期:1992-11-01 00:00:00

  • Flash Inactivation of Oxygen Evolution: IDENTIFICATION OF S(2) AS THE TARGET OF INACTIVATION BY TRIS.

    abstract::Brief saturating light flashes were used to probe the mechanism of inactivation of O(2) evolution by Tris in chloroplasts. Maximum inactivation with a single flash and an oscillation with period of four on subsequent flashes was observed. Analyses of the oscillations suggested that only the charge-collecting O(2)-evol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.4.735

    authors: Frasch WD,Cheniae GM

    更新日期:1980-04-01 00:00:00

  • Evidence for a Block between Plastoquinone and Cytochrome f in a Photosynthetic Mutant of Lemna with Abnormal Flowering Behavior.

    abstract::Mutant strain 1073 of Lemna perpusilla is concluded to be blocked between plastoquinone and cytochrome f in the photosynthetic electron transport system. The location of the block is based on the following observations of activities in chloroplasts isolated from the mutant and wild-type plants. (a) Relative to wild ty...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.4.577

    authors: Shahak Y,Posner HB,Avron M

    更新日期:1976-04-01 00:00:00

  • Salicylic Acid in Rice (Biosynthesis, Conjugation, and Possible Role).

    abstract::Salicylic acid (SA) is a natural inducer of disease resistance in some dicotyledonous plants. Rice seedlings (Oryza sativa L.) had the highest levels of SA among all plants tested for SA content (between 0.01 and 37.19 [mu]g/g fresh weight). The second leaf of rice seedlings had slightly lower SA levels than any young...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.633

    authors: Silverman P,Seskar M,Kanter D,Schweizer P,Metraux JP,Raskin I

    更新日期:1995-06-01 00:00:00

  • The activity of the chloroplastic Ndh complex is regulated by phosphorylation of the NDH-F subunit.

    abstract::Hydrogen peroxide (H(2)O(2)) induces increases, to different degrees, in transcripts, protein levels, and activity of the Ndh complex (EC 1.6.5.3). In the present work, we have compared the effects of relatively excess light, H(2)O(2), dimethylthiourea (a scavenger of H(2)O(2)), and/or EGTA (a Ca(2+) chelator) on the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.020321

    authors: Lascano HR,Casano LM,Martín M,Sabater B

    更新日期:2003-05-01 00:00:00

  • The relationship of the peroxidative indoleacetic Acid oxidase system to in vivo ethylene synthesis in cotton.

    abstract::Since peroxidase and manganese have been implicated in both auxin destruction and ethylene production, the effect of auxins and high tissue levels of manganese on the peroxidative indoleacetic acid oxidase system and the internal level of ethylene was determined in cotton (Gossypium hirsutum L. cv. Watson GL-7). The h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.4.555

    authors: Fowler JL,Morgan PW

    更新日期:1972-04-01 00:00:00

  • Two-Dimensional Electrophoretic Studies of the Proteins and Polypeptides in Mature Pollen Grains and the Male Germ Unit of Plumbago zeylanica.

    abstract::Three fractions (male germ unit [MGU]-rich, cytoplasmic-particulate and water-soluble proteins) were isolated from pollen of Plumbago zeylanica L. Proteins were extracted using a phenol procedure and polypeptide patterns were compared on one- and two-dimensional polyacrylamide gels. The MGU-rich fraction contains the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.764

    authors: Geltz NR,Russell SD

    更新日期:1988-11-01 00:00:00

  • Chlorophyll Formation and Photosynthetic Competence in Euglena During Light-Induced Chloroplast Development in the Presence of 3, (3,4-dichlorophenyl) 1,1-Dimethyl Urea (DCMU).

    abstract::The possibility that photosynthetic competence is gratuitous for light-induced chloroplast development in Euglena gracilis var. bacillaris was examined by incubating dark-grown resting cells in the light with DCMU, an inhibitor of photosynthesis. Under these conditions photosynthetic carbon dioxide fixation was inhibi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.12.1716

    authors: Schiff JA,Zeldin MH,Rubman J

    更新日期:1967-12-01 00:00:00

  • Sodium and potassium absorption by bean stem tissue.

    abstract::The effect of various periods of pretreatment in CaSO(4) solutions (aging) on the absorption of Na and K by bean stem slices was investigated. Freshly sliced tissue absorbed Na over the entire range of concentrations studied (0.02-50 mm). Potassium absorption by fresh tissue was nil at concentrations below 0.5 mm but ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.4.547

    authors: Rains DW

    更新日期:1969-04-01 00:00:00

  • Fat Metabolism in Higher Plants: XLVII. The Effect of Nitrite and Other Anions on the Formation of Unsaturated Fatty Acids by Isolated Chloroplasts.

    abstract::Intact spinach and barley chloroplast normally incorporate (14)C-acetate into palmitate and oleate as the major (14)C fatty acids. Addition of nitrite markedly altered the relative patterns of the products with the appearance of stearate, a drop in oleate, but no marked change in palmitate. Arsenite greatly increased ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.4.497

    authors: Kannangara CG,Stumpf PK

    更新日期:1972-04-01 00:00:00

  • Units of freezing of deep supercooled water in woody xylem.

    abstract::The low temperature exotherms (LTE) of 1-year-old twigs of Haralson apple (Malus pumila Mill.), shagbark hickory (Carya ovata [Mill.] K. Koch), green ash (Fraxinus pennsylvanica Marsh), honey locust (Gleditsia triacanthos L.), American chestnut (Castanea dentata [Marsh] Borkh.), and red oak (Quercus rubra L.) were det...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.1.40

    authors: Hong SG,Sucoff E

    更新日期:1980-07-01 00:00:00

  • Photosynthetic carbon metabolism of isolated corn chloroplasts.

    abstract::Chloroplasts have been isolated from 4- to 6-day-old corn (Zea mays) leaves capable of assimilating 45 micromoles CO(2) per milligram chlorophyll per hour. The effects of various factors such as inorganic phosphate, reducing agents, inhibitors, intermediates of the photosynthetic carbon reduction cycle, organic acids,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.4.607

    authors: O'neal D,Hew CS,Latzko E,Gibbs M

    更新日期:1972-04-01 00:00:00

  • Role of Ethylene in the Geotropic Response of Bermudagrass (Cynodon dactylon L. Pers.) Stolons.

    abstract::We studied the relationship between ethylene and gravity-induced upward bending of bermudagrass (Cynodon dactylon L. Pers.) stolons. Ethylene production begins within 3 hours of the onset of gravistimulation, and increases thereafter until the 15th hour, after which it declines. There is a close positive relationship ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.4.1251

    authors: Balatti PA,Willemöes JG

    更新日期:1989-12-01 00:00:00

  • Involvement of Singlet Oxygen in 5-Aminolevulinic Acid-Induced Photodynamic Damage of Cucumber (Cucumis sativus L.) Chloroplasts.

    abstract::Cucumber (Cucumis sativus L., cv Poinsette) plants were sprayed with 20 millimolar 5-aminolevulinic acid and then incubated in the dark for 14 hours. The intact chloroplasts were isolated from the above plants in the dark and were exposed to weak light (250 micromoles per square meter per second). Within 30 minutes, p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.1.7

    authors: Chakraborty N,Tripathy BC

    更新日期:1992-01-01 00:00:00

  • Genetic control of chloroplast pigment development in soybeans as a function of leaf and plant maturity.

    abstract::Changes in the major chloroplast pigments of pigment-deficient genotypes of soybeans were studied as a function of leaf age and plant age. The trends of pigment development are plotted as a function of relative leaf age at two periods of plant development. Comparisons are made between aging leaves from different nodes...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.4.759

    authors: Eskins K

    更新日期:1981-04-01 00:00:00

  • Arabidopsis putative selenium-binding protein1 expression is tightly linked to cellular sulfur demand and can reduce sensitivity to stresses requiring glutathione for tolerance.

    abstract::Selenium-Binding Protein1 (SBP1) gene expression was studied in Arabidopsis (Arabidopsis thaliana) seedlings challenged with several stresses, including cadmium (Cd), selenium {selenate [Se(VI)] and selenite [Se(IV)]}, copper (Cu), zinc (Zn), and hydrogen peroxide (H(2)O(2)) using transgenic lines expressing the lucif...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.144808

    authors: Hugouvieux V,Dutilleul C,Jourdain A,Reynaud F,Lopez V,Bourguignon J

    更新日期:2009-10-01 00:00:00

  • Auxin receptors of maize coleoptile membranes do not have ATPase activity.

    abstract::Membrane-localized auxin-binding sites from coleoptiles and primary leaves of Zea mays L. which may be auxin receptors can be fully solubilized by 1 to 1.5 mg of Triton X-100 per mg of membrane protein (about 1 mg per gram of original tissue fresh weight), while 70% of the basal (Mg(2+))-ATPase and 85% of the K(+)-sti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.4.581

    authors: Cross JW,Briggs WR

    更新日期:1978-04-01 00:00:00