A Seed Storage Protein with Possible Self-Affinity through Lectin-Like Binding.

Abstract:

:The primary storage protein of oat (Avena sativa L.) seeds, globulin, was shown to have a specific carbohydrate-binding activity. The globulin was capable of hemagglutinating rabbit red blood cells and this hemagglutination was inhibited by the beta-glucan, laminarin, as well as by carbohydrate which had been cleaved from the native globulin. Globulin with carbohydrate-binding activity was isolated from cell wall preparations and from defatted flour. The lectin activity apparently resides in the alpha-subunit of the globulin and has affinity for the carbohydrate which is O-glycosidically linked to the globulin. A portion of this carbohydrate is attached to the beta-subunit. Two affinity columns were synthesized utilizing laminarin and the carbohydrate from the native globulin as ligands. The hemagglutinating activity bound to both of these columns. The activity was specifically eluted from the globulin-carbohydrate affinity column with carbohydrate cleaved from native globulin by an alkali-catalyzed beta-elimination. The possible roles of this unique self-binding capacity are discussed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Langston-Unkefer PJ,Gade W

doi

10.1104/pp.74.3.675

subject

Has Abstract

pub_date

1984-03-01 00:00:00

pages

675-80

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

74

pub_type

杂志文章
  • Regulation of ADP-Glucose Pyrophosphorylase from Chlorella vulgaris.

    abstract::ADP-glucose pyrophosphorylase was partially purified from Chlorella vulgaris 11h. 3-Phosphoglycerate activated the enzyme by lowering the Michaelis constant for glucose-1-phosphate (from 0.97 to 0.36 millimolar in the presence of 2 millimolar phosphoglycerate) and ATP (from 0.23 to 0.10 millimolar), as well as increas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.3.601

    authors: Nakamura Y,Imamura M

    更新日期:1985-07-01 00:00:00

  • Potassium Transport in Corn Roots : III. Perturbation by Exogenous NADH and Ferricyanide.

    abstract::It has recently been reported that plasmalemma electron transport may be involved in the generation of H(+) gradients and the uptake of ions into root tissue. We report here on the influence of extracellular NADH and ferricyanide on K(+) ((86)Rb(+)) influx, K(+) ((86)Rb(+)) efflux, net apparent H(+) efflux, and O(2) c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.2.429

    authors: Kochian LV,Lucas WJ

    更新日期:1985-02-01 00:00:00

  • Oxalate exudation by taro in response to Al

    abstract::Roots of taro (Colocasia esculenta [L.] Schott cvs Bun-long and Lehua maoli) exuded increasing concentrations of oxalate with increasing Al stress. This exudation was a specific response to excess Al and not to P deficiency. Addition of oxalate to Al-containing solutions ameliorated the toxic effect of Al. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.3.861

    authors: Ma Z,Miyasaka SC

    更新日期:1998-11-01 00:00:00

  • In Vitro Prenylation of the Small GTPase Rac13 of Cotton.

    abstract::Previous work (D.P. Delmer, J. Pear, A. Andrawis, D. Stalker [1995] Mol Gen Genet 248: 43-51) has identified a gene in cotton (Gossypium hirsutum), Rac13, that encodes a small, signal-transducing GTPase and shows high expression in the fiber at the time of transition from primary to secondary wall synthesis. Since Rac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.4.1491

    authors: Trainin T,Shmuel M,Delmer DP

    更新日期:1996-12-01 00:00:00

  • Relationship between Nitrate Uptake, Flux, and Reduction and the Accumulation of Reduced Nitrogen in Maize (Zea mays L.): II. EFFECT OF NUTRIENT NITRATE CONCENTRATION.

    abstract::Two maize hybrids were grown under growth chamber conditions on solution or vermiculite medium that contained 2.5, 7.5, or 15 millimolar nitrate. The objectives were to determine: (a) the effect of nitrate supply on N metabolism and growth and (b) the interrelationship between nitrate uptake, flux, and reduction on th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.6.1184

    authors: Reed AJ,Hageman RH

    更新日期:1980-12-01 00:00:00

  • Endoplasmic Reticulum Formation during Germination of Wheat Seeds : A QUANTITATIVE ELECTRON MICROSCOPE STUDY.

    abstract::This study demonstrates germination-induced ultrastructural changes in wheat (Triticum aestivum L. cv Arthur) aleurone cells. Seeds imbided for 4 hours in water contained endoplasmic reticulum (ER) or ER-like membranes as vesicles or as short segments of membrane associated with the spherosomes on the periphery of ale...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1319

    authors: Buckhout TJ,Gripshover BM,Morré DJ

    更新日期:1981-12-01 00:00:00

  • Effect of Heat Shock on Plant Growth and on Lipid and beta-Glucan Syntheses.

    abstract::Primary leaves of 8-day-old Phaseolus vulgaris plants dipped in water at 46.5 to 47.5 C for 2 minutes were found to have decreased synthesis of lipid and beta-glucan compared to controls; UDP-glucose was used as substrate by cell-free preparations. As time elapsed, leaf growth, which was inhibited within the first 12 ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.1.118

    authors: Ordin L,Itai C,Benzioni A,Musolan C,Kindinger JI

    更新日期:1974-01-01 00:00:00

  • Abscisic acid deficiency causes changes in cuticle permeability and pectin composition that influence tomato resistance to Botrytis cinerea.

    abstract::A mutant of tomato (Solanum lycopersicum) with reduced abscisic acid (ABA) production (sitiens) exhibits increased resistance to the necrotrophic fungus Botrytis cinerea. This resistance is correlated with a rapid and strong hydrogen peroxide-driven cell wall fortification response in epidermis cells that is absent in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.158972

    authors: Curvers K,Seifi H,Mouille G,de Rycke R,Asselbergh B,Van Hecke A,Vanderschaeghe D,Höfte H,Callewaert N,Van Breusegem F,Höfte M

    更新日期:2010-10-01 00:00:00

  • Method for overcoming the antiethylene effects of ag.

    abstract::A technique is described for eliminating the antiethylene effects of the Ag(+) ion in the intact pea plant (Pisum sativum). The technique is based on the ability of the ethylene mimic, acetylene, to negate the antiethylene effect of Ag(+), presumably through salt formation, and subsequently to induce the ethylene resp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.4.616

    authors: Beyer EM

    更新日期:1978-10-01 00:00:00

  • Effects of sulfanilamide and methotrexate on 13C fluxes through the glycine decarboxylase/serine hydroxymethyltransferase enzyme system in arabidopsis.

    abstract::In C3 plants large amounts of photorespiratory glycine (Gly) are converted to serine by the tetrahydrofolate (THF)-dependent activities of the Gly decarboxylase complex (GDC) and serine hydroxymethyltransferase (SHMT). Using 13C nuclear magnetic resonance, we monitored the flux of carbon through the GDC/SHMT enzyme sy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.1.137

    authors: Prabhu V,Chatson KB,Lui H,Abrams GD,King J

    更新日期:1998-01-01 00:00:00

  • Confounding of alternate respiration by lipoxygenase activity.

    abstract::The initial burst of respiratory activity (Q(o) (2)) of imbibing soybean (Glycine max [L.] Merr. var. Wayne) seed tissue is cyanide-insensitive, and sensitive to salicylhydroxamate: presumptive evidence for the presence of alternate respiration. The initial O(2) consumption is also highly sensitive to propyl gallate. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.3.470

    authors: Parrish DJ,Leopold AC

    更新日期:1978-09-01 00:00:00

  • Natural variation in sensitivity to a loss of chloroplast translation in Arabidopsis.

    abstract::Mutations that eliminate chloroplast translation in Arabidopsis (Arabidopsis thaliana) result in embryo lethality. The stage of embryo arrest, however, can be influenced by genetic background. To identify genes responsible for improved growth in the absence of chloroplast translation, we examined seedling responses of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.249052

    authors: Parker N,Wang Y,Meinke D

    更新日期:2014-12-01 00:00:00

  • Effect of Growth Temperature on the Lipid and Fatty Acid Composition, and the Dependence on Temperature of Light-induced Redox Reactions of Cytochrome f and of Light Energy Redistribution in the Thermophilic Blue-Green Alga Synechococcus lividus.

    abstract::The thermophilic blue-green alga Synechococcus lividus was grown at 55 and 38 C. Arrhenius plots of the transient reduction of cytochrome during actinic illumination with light that excited both pigment systems revealed breaks near 43 and 26 C for cells grown at 55 C. In cells grown at 38 C these breaks occurred near ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.3.524

    authors: Fork DC

    更新日期:1979-03-01 00:00:00

  • Phosphatidylcholine Synthesis in Castor Bean Endosperm : I. Metabolism of l-Serine.

    abstract::Endosperm halves from 3-day-old castor bean (Ricinus communis var Hale) were incubated for 30 minutes with l-[(14)C]serine, after which label was observed in ethanolamine, choline, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, ethanolaminephosphate, and CDPethanolamine, but not in cholinephosphate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.1.78

    authors: Kinney AJ,Moore TS

    更新日期:1987-05-01 00:00:00

  • The Plastid Genome of Polytoma uvella Is the Largest Known among Colorless Algae and Plants and Reflects Contrasting Evolutionary Paths to Nonphotosynthetic Lifestyles.

    abstract::The loss of photosynthesis is frequently associated with parasitic or pathogenic lifestyles, but it also can occur in free-living, plastid-bearing lineages. A common consequence of becoming nonphotosynthetic is the reduction in size and gene content of the plastid genome. In exceptional circumstances, it can even resu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01628

    authors: Figueroa-Martinez F,Nedelcu AM,Smith DR,Reyes-Prieto A

    更新日期:2017-02-01 00:00:00

  • Oxygen-18 and deuterium labeling studies of choline oxidation by spinach and sugar beet.

    abstract::Chenopods synthesize betaine by a two-step oxidation of choline: choline --> betaine aldehyde --> betaine. The pathway is chloroplastic; the first step has been shown in isolated spinach (Spinacia oleracea L.) chloroplasts to be O(2)- and light-dependent, the role of light being to provide reducing power (P Weigel, EA...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.695

    authors: Lerma C,Hanson AD,Rhodes D

    更新日期:1988-11-01 00:00:00

  • Effect of Inhibition of Glycosylation on the Appearance of a 60 kD Membrane Glycopolypeptide in Endomembrane Fractions of Soybean Root.

    abstract::Endomembrane (endoplasmic reticulum, Golgi apparatus, plasma membrane) proteins of soybean (Glycine max) root cells are highly glycosylated. We investigated whether N-linked oligosaccharide moieties are essential for the correct intracellular transport of plant endomembrane glycoproteins. Excised roots were incubated ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.1.299

    authors: Lafayette PR,Travis RL

    更新日期:1989-01-01 00:00:00

  • Xylem sap flow as affected by metabolic inhibitors and girdling.

    abstract::The flow of sap in Populus tremuloides was studied by the thermoelectric method immediately prior to and at several periods of time after radical girdling. Similarly, the flow of sap in Betula papyrifera was studied before and after girdling or injection with metabolic inhibitors or a filtrate of a culture of Ceratocy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.4.641

    authors: Kurtzman RH

    更新日期:1966-04-01 00:00:00

  • Alfalfa Root Nodule Carbon Dioxide Fixation : II. Partial Purification and Characterization of Root Nodule Phosphoenolpyruvate Carboxylase.

    abstract::A nonphotosynthetic phosphoenolpyruvate carboxylase (EC 4.1.1.31) was partially purified from the cytosol of root nodules of alfalfa. The enzyme was purified 86-fold by ammonium sulfate fractionation, DEAE-cellulose, hydroxylapatite chromatography, and reactive agarose with a final yield of 32%. The enzyme exhibited a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.1.261

    authors: Vance CP,Stade S

    更新日期:1984-05-01 00:00:00

  • Development of photochemical activity in relation to pigment and membrane protein accumulation in chloroplasts of barley and its virescens mutant.

    abstract::The development of photochemical activity in relation to pigment and membrane protein accumulation in chloroplasts of greening wild-type barley (Hordeum vulgare L. cv. Gateway) and its virescens mutant were studied. The rate of chlorophyll accumulation per plastid was faster in the wild-type than in the mutant seedlin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.6.1392

    authors: Kyle DJ,Zalik S

    更新日期:1982-06-01 00:00:00

  • An egg apparatus-specific enhancer of Arabidopsis, identified by enhancer detection.

    abstract::Despite a central role in angiosperm reproduction, few gametophyte-specific genes and promoters have been isolated, particularly for the inaccessible female gametophyte (embryo sac). Using the Ds-based enhancer-detector line ET253, we have cloned an egg apparatus-specific enhancer (EASE) from Arabidopsis (Arabidopsis ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.068262

    authors: Yang W,Jefferson RA,Huttner E,Moore JM,Gagliano WB,Grossniklaus U

    更新日期:2005-11-01 00:00:00

  • Role of Potassium in Carbon Dioxide Assimilation in Medicago sativa L.

    abstract::Alfalfa was grown hydroponically in 0, 0.6, and 4.8 millimolar K in order to determine the influence of tissue level of K on photosynthesis, dark respiration, photorespiration, stomatal and mesophyll resistance to CO(2), photosystem I and II activity, and synthesis and activity of ribulose 1,5-bisphosphate carboxylase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.5.878

    authors: Peoples TR,Koch DW

    更新日期:1979-05-01 00:00:00

  • Branching Mutant rms-2 in Pisum sativum (Grafting Studies and Endogenous Indole-3-Acetic Acid Levels).

    abstract::Isogenic lines of pea (Pisum sativum L.) were used to determine the physiological site of action of the Rms-2 gene, which maintains apical dominance, and its effect on endogenous free indole-3-acetic acid (IAA) levels. In mutant rms-2 scions, which normally produce lateral branches below node 3 and above node 7, apica...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.3.953

    authors: Beveridge CA,Ross JJ,Murfet IC

    更新日期:1994-03-01 00:00:00

  • Chorismate Mutase Isoenzymes from Selected Plants and Their Immunological Comparison with the Isoenzymes from Sorghum bicolor.

    abstract::The isoenzyme pattern of chorismate mutase (EC 5.4.99.5) was examined by diethylaminoethyl-cellulose chromatography in a wide variety of plants. All plants contained a regulated form of chorismate mutase (CM-1), and most contained an additional, unregulated form (CM-2). The regulatory properties of CM-1 differed signi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.3.717

    authors: Singh BK,Lonergan SG,Conn EE

    更新日期:1986-07-01 00:00:00

  • Replication of DNA by nuclei isolated from soybean suspension cultures.

    abstract::DNA replication was studied in nuclei isolated from soybean cells grown in suspension culture. The isolation procedure involved the preparation of protoplasts, their lysis with a nonionic detergent and purification of nuclei. These nuclei synthesized low molecular weight DNA and joined these fragments into DNA of inte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.4.726

    authors: Roman R

    更新日期:1980-10-01 00:00:00

  • Endogenous NO(3) in the Root as a Source of Substrate for Reduction in the Light.

    abstract::An experiment was conducted to investigate the reduction of endogenous NO(3) (-), which had been taken up by plants in darkness, during the course of the subsequent light period. Vegetative, nonnodulated soybean plants (Glycine max [L]. Merrill, ;Ransom') were exposed to 1.0 millimolar (15)NO(3) (-) for 12 hours in da...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.4.1421

    authors: Rufty TW,Volk RJ,Mackown CT

    更新日期:1987-08-01 00:00:00

  • Long-Term Growth of Moss in Microfluidic Devices Enables Subcellular Studies in Development.

    abstract::Key developmental processes that occur on the subcellular and cellular level or occur in occluded tissues are difficult to access, let alone image and analyze. Recently, culturing living samples within polydimethylsiloxane (PDMS) microfluidic devices has facilitated the study of hard-to-reach developmental events. Her...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00879

    authors: Bascom CS Jr,Wu SZ,Nelson K,Oakey J,Bezanilla M

    更新日期:2016-09-01 00:00:00

  • Abscisic Acid and the Photoperiodic Induction of Dormancy in Salix viminalis L.

    abstract::A series of growth room experiments was carried out aiming to establish the role of abscisic acid on dormancy of Salix viminalis L. The inhibitor content and abscisic acid levels of extracts from roots, sap, leaves, and apical tissues of willow were measured using biological assay and gas-liquid chromatography.No evid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.4.774

    authors: Alvim R,Saunders PF,Barros RS

    更新日期:1979-04-01 00:00:00

  • Interactions between potassium and calcium in their absorption by intact barley plants. I. Effects of potassium on calcium absorption.

    abstract::Increasing concentrations of K (20, 200, 2000 mum) in the nutrient solution depressed Ca content and concentration in barley plants growing in nutrient solutions of low Ca concentrations (250 and 2500 mum). Increasing K from 20 to 200 mum depressed Ca absorption more than increasing K from 200 to 2000 mum K.The strong...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.10.1717

    authors: Johansen C,Edwards DG,Loneragan JF

    更新日期:1968-10-01 00:00:00

  • Mode of Action Studies on Nitrodiphenyl Ether Herbicides : II. The Role of Photosynthetic Electron Transport in Scenedesmus obliquus.

    abstract::The nitrodiphenyl ether herbicide 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitroacetophenone oxime-o-(acetic acid, methyl ester) (DPEI) induces light- and O(2)-dependent lipid peroxidation and chlorophyll (Chl) bleaching in the green alga Scenedesmus obliquus. Under conditions of O(2)-limitation, these effects are di...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.2.674

    authors: Bowyer JR,Hallahan BJ,Camilleri P,Howard J

    更新日期:1989-02-01 00:00:00