Long-Term Growth of Moss in Microfluidic Devices Enables Subcellular Studies in Development.

Abstract:

:Key developmental processes that occur on the subcellular and cellular level or occur in occluded tissues are difficult to access, let alone image and analyze. Recently, culturing living samples within polydimethylsiloxane (PDMS) microfluidic devices has facilitated the study of hard-to-reach developmental events. Here, we show that an early diverging land plant, Physcomitrella patens, can be continuously cultured within PDMS microfluidic chambers. Because the PDMS chambers are bonded to a coverslip, it is possible to image P. patens development at high resolution over long time periods. Using PDMS chambers, we report that wild-type protonemal tissue grows at the same rate as previously reported for growth on solid medium. Using long-term imaging, we highlight key developmental events, demonstrate compatibility with high-resolution confocal microscopy, and obtain growth rates for a slow-growing mutant. By coupling the powerful genetic tools available to P. patens with long-term growth and imaging provided by PDMS microfluidic chambers, we demonstrate the capability to study cellular and subcellular developmental events in plants directly and in real time.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Bascom CS Jr,Wu SZ,Nelson K,Oakey J,Bezanilla M

doi

10.1104/pp.16.00879

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

28-37

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.16.00879

journal_volume

172

pub_type

杂志文章
  • Strigolactone acts downstream of auxin to regulate bud outgrowth in pea and Arabidopsis.

    abstract::During the last century, two key hypotheses have been proposed to explain apical dominance in plants: auxin promotes the production of a second messenger that moves up into buds to repress their outgrowth, and auxin saturation in the stem inhibits auxin transport from buds, thereby inhibiting bud outgrowth. The recent...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.134783

    authors: Brewer PB,Dun EA,Ferguson BJ,Rameau C,Beveridge CA

    更新日期:2009-05-01 00:00:00

  • Antisense suppression of 2-cysteine peroxiredoxin in Arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism.

    abstract::The aim of this study was to characterize the effect of decreased 2-cysteine peroxiredoxin (2-CP) on the leaf anti-oxidative system in Arabidopsis. At three stages of leaf development, two lines of transgenic Arabidopsis mutants with decreased contents of chloroplast 2-CP were compared with wild type and a control lin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.2.823

    authors: Baier M,Noctor G,Foyer CH,Dietz KJ

    更新日期:2000-10-01 00:00:00

  • The Acyl Desaturase CER17 Is Involved in Producing Wax Unsaturated Primary Alcohols and Cutin Monomers.

    abstract::We report n-6 monounsaturated primary alcohols (C26:1, C28:1, and C30:1 homologs) in the cuticular waxes of Arabidopsis (Arabidopsis thaliana) inflorescence stem, a class of wax not previously reported in Arabidopsis. The Arabidopsis cer17 mutant was completely deficient in these monounsaturated alcohols, and CER17 wa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01956

    authors: Yang X,Zhao H,Kosma DK,Tomasi P,Dyer JM,Li R,Liu X,Wang Z,Parsons EP,Jenks MA,Lü S

    更新日期:2017-02-01 00:00:00

  • Pathway-level acceleration of glycogen catabolism by a response regulator in the cyanobacterium Synechocystis species PCC 6803.

    abstract::Response regulators of two-component systems play pivotal roles in the transcriptional regulation of responses to environmental signals in bacteria. Rre37, an OmpR-type response regulator, is induced by nitrogen depletion in the unicellular cyanobacterium Synechocystis species PCC 6803. Microarray and quantitative rea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.232025

    authors: Osanai T,Oikawa A,Numata K,Kuwahara A,Iijima H,Doi Y,Saito K,Hirai MY

    更新日期:2014-04-01 00:00:00

  • Isolation of Guard Cell Protoplasts from Mechanically Prepared Epidermis of Vicia faba Leaves.

    abstract::A method for isolating guard cell protoplasts (GCP) from mechanically prepared epidermis of Vicia faba is described. Epidermis was prepared by homogenizing leaves in a Waring blender in a solution of 10% Ficoll, 5 millimolar CaCl(2), and 0.1% polyvinylpyrrolidone 40 (PVP). Attached mesophyll and epidermal cells were r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.4.1382

    authors: Kruse T,Tallman G,Zeiger E

    更新日期:1989-08-01 00:00:00

  • Suppression of a vegetative MADS box gene of potato activates axillary meristem development.

    abstract::Potato MADS box 1 (POTM1) is a member of the SQUAMOSA-like family of plant MADS box genes isolated from an early stage tuber cDNA library. The RNA of POTM1 is most abundant in vegetative meristems of potato (Solanum tuberosum), accumulating specifically in the tunica and corpus layers of the meristem, the procambium, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.012500

    authors: Rosin FM,Hart JK,Van Onckelen H,Hannapel DJ

    更新日期:2003-04-01 00:00:00

  • Protein cryoprotective activity of a cytosolic small heat shock protein that accumulates constitutively in chestnut stems and is up-regulated by low and high temperatures.

    abstract::Heat shock, and other stresses that cause protein misfolding and aggregation, trigger the accumulation of heat shock proteins (HSPs) in virtually all organisms. Among the HSPs of higher plants, those belonging to the small HSP (sHSP) family remain the least characterized in functional terms. We analyzed the occurrence...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.035857

    authors: Lopez-Matas MA,Nuñez P,Soto A,Allona I,Casado R,Collada C,Guevara MA,Aragoncillo C,Gomez L

    更新日期:2004-04-01 00:00:00

  • Inositol polyphosphate 5-phosphatases 1 and 2 are required for regulating seedling growth.

    abstract::Signals can be perceived and amplified at the cell membrane by receptors coupled to the production of a variety of second messengers, including myoinositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]. The myoinositol polyphosphate 5-phosphatases (5PTases; EC 3.1.3.56) comprise a large protein family that hydrolyzes 5-phospha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.089474

    authors: Gunesekera B,Torabinejad J,Robinson J,Gillaspy GE

    更新日期:2007-03-01 00:00:00

  • Regulation of Nicotine Biosynthesis by an Endogenous Target Mimicry of MicroRNA in Tobacco.

    abstract::The interaction between noncoding endogenous target mimicry (eTM) and its corresponding microRNA (miRNA) is a newly discovered regulatory mechanism and plays pivotal roles in various biological processes in plants. Tobacco (Nicotiana tabacum) is a model plant for studying secondary metabolite alkaloids, of which nicot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00649

    authors: Li F,Wang W,Zhao N,Xiao B,Cao P,Wu X,Ye C,Shen E,Qiu J,Zhu QH,Xie J,Zhou X,Fan L

    更新日期:2015-10-01 00:00:00

  • Extremely low frequency resistance fluctuations of chara membrane.

    abstract::The membrane resistivity of resting characean cells was measured and found to fluctuate unpredictably about its resting level. The amplitude of these fluctuations relative to the mean resistivity was normally somewhat larger than the same ratio for the vacuolar voltage; further the resistivity and voltage fluctuations...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.723

    authors: Gokhale AV,Pickard WF

    更新日期:1985-11-01 00:00:00

  • Combining quantitative trait Loci analysis and an ecophysiological model to analyze the genetic variability of the responses of maize leaf growth to temperature and water deficit.

    abstract::Ecophysiological models predict quantitative traits of one genotype in any environment, whereas quantitative trait locus (QTL) models predict the contribution of alleles to quantitative traits under a limited number of environments. We have combined both approaches by dissecting into effects of QTLs the parameters of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.013839

    authors: Reymond M,Muller B,Leonardi A,Charcosset A,Tardieu F

    更新日期:2003-02-01 00:00:00

  • Isolation of Sperms from the Pollen Tubes of Flowering Plants during Fertilization.

    abstract::Sperm cells have been isolated from pollen tubes growing in style segments of the dicotlyledon Rhododendron macgregoriae and the monocotyledon Gladiolus gandavensis by the in vivo/in vitro method at various stages of fertilization. Pollen tubes emerged from the cut end of the style into agar medium, and more than 95% ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.3.647

    authors: Shivanna KR,Xu H,Taylor P,Knox RB

    更新日期:1988-07-01 00:00:00

  • Distinct Mechanisms of the ORANGE Protein in Controlling Carotenoid Flux.

    abstract::β-Carotene adds nutritious value and determines the color of many fruits, including melon (Cucumis melo). In melon mesocarp, β-carotene accumulation is governed by the Orange gene (CmOr) golden single-nucleotide polymorphism (SNP) through a yet to be discovered mechanism. In Arabidopsis (Arabidopsis thaliana), OR incr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01256

    authors: Chayut N,Yuan H,Ohali S,Meir A,Sa'ar U,Tzuri G,Zheng Y,Mazourek M,Gepstein S,Zhou X,Portnoy V,Lewinsohn E,Schaffer AA,Katzir N,Fei Z,Welsch R,Li L,Burger J,Tadmor Y

    更新日期:2017-01-01 00:00:00

  • Influx of na, k, and ca into roots of salt-stressed cotton seedlings : effects of supplemental ca.

    abstract::High Na(+) concentrations may disrupt K(+) and Ca(2+) transport and interfere with growth of many plant species, cotton (Gossypium hirsutum L.) included. Elevated Ca(2+) levels often counteract these consequences of salinity. The effect of supplemental Ca(2+) on influx of Ca(2+), K(+), and Na(+) in roots of intact, sa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.3.510

    authors: Cramer GR,Lynch J,Läuchli A,Epstein E

    更新日期:1987-03-01 00:00:00

  • Isolation of a kaurene synthetase inhibitor from castor bean seedlings and cell suspension cultures.

    abstract::Biosynthesis of ent-kaurene was investigated in extracts of cell suspension cultures and seedlings of castor bean. Both cell-free extracts contain an inhibitor of kaurene synthetase. The inhibition affects mainly the cyclization of geranylgeranyl pyrophosphate to copalyl pyrophosphate (activity A) and has little or no...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.6.1169

    authors: Gafni Y,Shechter I

    更新日期:1981-06-01 00:00:00

  • Glutamine metabolism in corn kernels cultured in vitro.

    abstract::The fate of glutamine, the major source of nitrogen supplied to the developing maize endosperm, has been examined in endosperm tissues of corn caryopsis grown under sterile conditions. In the culture system, [U-(14)C]glutamine was included in the medium or was injected directly into the endosperm. Samples were harvest...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.3.520

    authors: Misra S,Oaks A

    更新日期:1985-03-01 00:00:00

  • Nickel in Plants: II. Distribution and Chemical Form in Soybean Plants.

    abstract::The gross tissue distribution, intracellular fate, and chemical behavior of Ni(2+) in soybean plants (Glycine max cv. Williams) were investigated. Following root absorption, Ni was highly mobile in the plant, with leaves being the major sink in the shoots for Ni during vegetative growth. A senescence >70% of the Ni pr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.4.566

    authors: Cataldo DA,Garland TR,Wildung RE

    更新日期:1978-10-01 00:00:00

  • Specificity of deoxyribonucleic Acid intercalating compounds in the control of phenylalanine ammonia lyase and pisatin levels.

    abstract::Compounds with planar triple ring systems such as acridine orange, 9-amino acridine, 9-amino-1,2,3,4-tetrahydroacridine (tacrine), 6,9-diamino-2-ethoxyacridine lactate monohydrate (DE-acridine), 6-chloro-9-(3'-diethylamino-2'-hydroxypropylamino) -2-methoxyacridine.2 HCl (CDM-acridine), quinacrine, 6-chloro-9-(4'-dieth...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.3.346

    authors: Hadwiger LA,Schwochau ME

    更新日期:1971-03-01 00:00:00

  • RML1 and RML2, Arabidopsis genes required for cell proliferation at the root tip.

    abstract::New cells are produced from the meristematic tissues located at the shoot and root tip throughout the life of higher plants. To investigate the genetic mechanism regulating meristematic activity, we isolated and characterized four single-gene, recessive mutants in Arabidopsis thaliana called root meristemless (rml). C...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.2.365

    authors: Cheng JC,Seeley KA,Sung ZR

    更新日期:1995-02-01 00:00:00

  • Strigolactone Hormones and Their Stereoisomers Signal through Two Related Receptor Proteins to Induce Different Physiological Responses in Arabidopsis.

    abstract::Two α/β-fold hydrolases, KARRIKIN INSENSITIVE2 (KAI2) and Arabidopsis thaliana DWARF14 (AtD14), are necessary for responses to karrikins (KARs) and strigolactones (SLs) in Arabidopsis (Arabidopsis thaliana). Although KAI2 mediates responses to KARs and some SL analogs, AtD14 mediates SL but not KAR responses. To furth...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.240036

    authors: Scaffidi A,Waters MT,Sun YK,Skelton BW,Dixon KW,Ghisalberti EL,Flematti GR,Smith SM

    更新日期:2014-07-01 00:00:00

  • Possible involvement of calmodulin and the cytoskeleton in electrofusion of plant protoplasts.

    abstract::Calmodulin antagonists, trifluoperazine, chlorpromazine, calmidazolium, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), strongly inhibited the electrofusion of barley (Hordeum vulgare L. cv Moor) protoplasts with a marked increase of broken fusion products, after 60 minutes of incubation. W-5, a dechlorinate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.4.1151

    authors: Abe S,Takeda J

    更新日期:1986-08-01 00:00:00

  • Scopoletin 8-Hydroxylase-Mediated Fraxetin Production Is Crucial for Iron Mobilization.

    abstract::Iron (Fe) is an essential mineral nutrient and an important factor for the composition of natural plant communities. Low Fe availability in aerated soils with neutral or alkaline pH has led to the evolution of elaborate mechanisms that extract Fe from the soil solution. In Arabidopsis (Arabidopsis thaliana), Fe is acq...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.00178

    authors: Tsai HH,Rodríguez-Celma J,Lan P,Wu YC,Vélez-Bermúdez IC,Schmidt W

    更新日期:2018-05-01 00:00:00

  • The purple cauliflower arises from activation of a MYB transcription factor.

    abstract::Anthocyanins are responsible for the color of many flowers, fruits, and vegetables. An interesting and unique Purple (Pr) gene mutation in cauliflower (Brassica oleracea var botrytis) confers an abnormal pattern of anthocyanin accumulation, giving the striking mutant phenotype of intense purple color in curds and a fe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.164160

    authors: Chiu LW,Zhou X,Burke S,Wu X,Prior RL,Li L

    更新日期:2010-11-01 00:00:00

  • Maize Photosystem I : Identification of the Subunit which Binds Plastocyanin.

    abstract::Photosystem I (PSI) has been isolated from mesophyll chloroplasts of mature maize leaves. The isolated PSI (PSI-200) was used as starting material for preparing an antenna-depleted core (PSI-100). Both of these preparations appear to be quite analogous to PSI complexes isolated from other plant tissue sources, such as...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.1.445

    authors: Wynn RM,Luong C,Malkin R

    更新日期:1989-09-01 00:00:00

  • Inositol Metabolism in Plants. III. Conversion of Myo-inositol-2-H to Cell Wall Polysaccharides in Sycamore (Acer pseudoplatanus L.) Cell Culture.

    abstract::Prolonged growth of cell cultures of sycamore (Acer pseudoplatanus L.) on agar medium containing myo-inositol-2-(3)H resulted in incorporation of label predominately into uronosyl and pentosyl units of cell wall polysaccharides. Procedures normally used to distinguish between pectic substance and hemicellulose yielded...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.9.1489

    authors: Roberts RM,Loewus F

    更新日期:1966-11-01 00:00:00

  • The U-Box/ARM E3 ligase PUB13 regulates cell death, defense, and flowering time in Arabidopsis.

    abstract::The components in plant signal transduction pathways are intertwined and affect each other to coordinate plant growth, development, and defenses to stresses. The role of ubiquitination in connecting these pathways, particularly plant innate immunity and flowering, is largely unknown. Here, we report the dual roles for...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.192617

    authors: Li W,Ahn IP,Ning Y,Park CH,Zeng L,Whitehill JG,Lu H,Zhao Q,Ding B,Xie Q,Zhou JM,Dai L,Wang GL

    更新日期:2012-05-01 00:00:00

  • Chemiluminescence in Wounded Root Tissue : EVIDENCE FOR PEROXIDASE INVOLVEMENT.

    abstract::Root and stem segments from soybean (Merrill cv. ;Bragg') showed an enhanced chemiluminescence upon mechanical injury. Roots emitted more light than did stems. Light emission was diminished by CN(-) and N(3) (-) but was not affected by rotenone and antimycin A. Catalase quenched chemiluminescence in wounded root segme...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.1.43

    authors: Salin ML,Bridges SM

    更新日期:1981-01-01 00:00:00

  • Characterization of a granule-bound starch synthase isoform found in the pericarp of wheat.

    abstract::Waxy wheat (Triticum aestivum L.) lacks the waxy protein, which is also known as granule-bound starch synthase I (GBSSI). The starch granules of waxy wheat endosperm and pollen do not contain amylose and therefore stain red-brown with iodine. However, we observed that starch from pericarp tissue of waxy wheat stained ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.2.451

    authors: Nakamura T,Vrinten P,Hayakawa K,Ikeda J

    更新日期:1998-10-01 00:00:00

  • Relative insensitivity of mitochondria in hardened and nonhardened rye coleoptile cells to freezing in situ.

    abstract::Mitochondria were isolated from excised coleoptiles of hardened and nonhardened winter rye (Secale cereale L. cv. Puma) seedlings which had been frozen extracellularly to different temperatures. No significant differences in the respiratory functions (ADP/O and respiratory control) were observed between mitochondria i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.5.713

    authors: Singh J,de la Roche AI,Siminovitch D

    更新日期:1977-11-01 00:00:00

  • Characterization of the branched-chain amino acid aminotransferase enzyme family in tomato.

    abstract::Branched-chain amino acids (BCAAs) are synthesized in plants from branched-chain keto acids, but their metabolism is not completely understood. The interface of BCAA metabolism lies with branched-chain aminotransferases (BCAT) that catalyze both the last anabolic step and the first catabolic step. In this study, six B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.154922

    authors: Maloney GS,Kochevenko A,Tieman DM,Tohge T,Krieger U,Zamir D,Taylor MG,Fernie AR,Klee HJ

    更新日期:2010-07-01 00:00:00