Abstract:
:Key developmental processes that occur on the subcellular and cellular level or occur in occluded tissues are difficult to access, let alone image and analyze. Recently, culturing living samples within polydimethylsiloxane (PDMS) microfluidic devices has facilitated the study of hard-to-reach developmental events. Here, we show that an early diverging land plant, Physcomitrella patens, can be continuously cultured within PDMS microfluidic chambers. Because the PDMS chambers are bonded to a coverslip, it is possible to image P. patens development at high resolution over long time periods. Using PDMS chambers, we report that wild-type protonemal tissue grows at the same rate as previously reported for growth on solid medium. Using long-term imaging, we highlight key developmental events, demonstrate compatibility with high-resolution confocal microscopy, and obtain growth rates for a slow-growing mutant. By coupling the powerful genetic tools available to P. patens with long-term growth and imaging provided by PDMS microfluidic chambers, we demonstrate the capability to study cellular and subcellular developmental events in plants directly and in real time.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Bascom CS Jr,Wu SZ,Nelson K,Oakey J,Bezanilla Mdoi
10.1104/pp.16.00879subject
Has Abstractpub_date
2016-09-01 00:00:00pages
28-37issue
1eissn
0032-0889issn
1532-2548pii
pp.16.00879journal_volume
172pub_type
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