Possible involvement of calmodulin and the cytoskeleton in electrofusion of plant protoplasts.

Abstract:

:Calmodulin antagonists, trifluoperazine, chlorpromazine, calmidazolium, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), strongly inhibited the electrofusion of barley (Hordeum vulgare L. cv Moor) protoplasts with a marked increase of broken fusion products, after 60 minutes of incubation. W-5, a dechlorinated analog of W-7, was found less effective for the inhibition than W-7. Ethyleneglycol-bis(beta- aminoethylether)-N,N'-tetraacetic acid a Ca(2+) chelator, La(3+), a surface Ca(2+) antagonist, and verapamil, a Ca(2+) channel blocker, also inhibited electrofusion. Dielectrophoresis was inhibited by La(3+). A microtubule inhibitor, vinblastine, inhibited electrofusion strongly while colchicine, slightly. A microfilament inhibitor, cytochalasin B, promoted fused cells to become spherical while phalloidin did not affect electrofusion.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Abe S,Takeda J

doi

10.1104/pp.81.4.1151

subject

Has Abstract

pub_date

1986-08-01 00:00:00

pages

1151-5

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

81

pub_type

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