Abstract:
:Calmodulin antagonists, trifluoperazine, chlorpromazine, calmidazolium, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), strongly inhibited the electrofusion of barley (Hordeum vulgare L. cv Moor) protoplasts with a marked increase of broken fusion products, after 60 minutes of incubation. W-5, a dechlorinated analog of W-7, was found less effective for the inhibition than W-7. Ethyleneglycol-bis(beta- aminoethylether)-N,N'-tetraacetic acid a Ca(2+) chelator, La(3+), a surface Ca(2+) antagonist, and verapamil, a Ca(2+) channel blocker, also inhibited electrofusion. Dielectrophoresis was inhibited by La(3+). A microtubule inhibitor, vinblastine, inhibited electrofusion strongly while colchicine, slightly. A microfilament inhibitor, cytochalasin B, promoted fused cells to become spherical while phalloidin did not affect electrofusion.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Abe S,Takeda Jdoi
10.1104/pp.81.4.1151subject
Has Abstractpub_date
1986-08-01 00:00:00pages
1151-5issue
4eissn
0032-0889issn
1532-2548journal_volume
81pub_type
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