Abstract:
:The interaction between noncoding endogenous target mimicry (eTM) and its corresponding microRNA (miRNA) is a newly discovered regulatory mechanism and plays pivotal roles in various biological processes in plants. Tobacco (Nicotiana tabacum) is a model plant for studying secondary metabolite alkaloids, of which nicotine accounts for approximately 90%. In this work, we identified four unique tobacco-specific miRNAs that were predicted to target key genes of the nicotine biosynthesis and catabolism pathways and an eTM, novel tobacco miRNA (nta)-eTMX27, for nta-miRX27 that targets QUINOLINATE PHOSPHORIBOSYLTRANSFERASE2 (QPT2) encoding a quinolinate phosphoribosyltransferase. The expression level of nta-miRX27 was significantly down-regulated, while that of QPT2 and nta-eTMX27 was significantly up-regulated after topping, and consequently, nicotine content increased in the topping-treated plants. The topping-induced down-regulation of nta-miRX27 and up-regulation of QPT2 were only observed in plants with a functional nta-eTMX27 but not in transgenic plants containing an RNA interference construct targeting nta-eTMX27. Our results demonstrated that enhanced nicotine biosynthesis in the topping-treated tobacco plants is achieved by nta-eTMX27-mediated inhibition of the expression and functions of nta-miRX27. To our knowledge, this is the first report about regulation of secondary metabolite biosynthesis by an miRNA-eTM regulatory module in plants.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Li F,Wang W,Zhao N,Xiao B,Cao P,Wu X,Ye C,Shen E,Qiu J,Zhu QH,Xie J,Zhou X,Fan Ldoi
10.1104/pp.15.00649subject
Has Abstractpub_date
2015-10-01 00:00:00pages
1062-71issue
2eissn
0032-0889issn
1532-2548pii
pp.15.00649journal_volume
169pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Spruce (Picea spp.) and other conifers employ terpenoid-based oleoresin as part of their defense against herbivores and pathogens. The short-chain isoprenyl diphosphate synthases (IDS) are situated at critical branch points in terpene biosynthesis, producing the precursors of the different terpenoid classes. To determ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.228940
更新日期:2014-02-01 00:00:00
abstract::Much of the ADP-Glc required for starch synthesis in the plastids of cereal endosperm is synthesized in the cytosol and transported across the plastid envelope. To provide information on the nature and role of the plastidial ADP-Glc transporter in barley (Hordeum vulgare), we screened a collection of low-starch mutant...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.045203
更新日期:2004-08-01 00:00:00
abstract::Plants undergoing heat and low-CO2 stresses emit large amounts of volatile isoprenoids compared with those in stress-free conditions. One hypothesis posits that the balance between reducing power availability and its use in carbon assimilation determines constitutive isoprenoid emission rates in plants and potentially...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.246207
更新日期:2014-10-01 00:00:00
abstract::Transgenic Flaveria bidentis (a C4 species) plants with an antisense gene directed against the mRNA of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) were used to examine the relationship between the CO2 assimilation rate, Rubisco content, and carbon isotope discrimination. Reduction in the amount of Rubisc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.2.469
更新日期:1997-02-01 00:00:00
abstract::Ethylene evolved from monoecious and gynoecious cucumber (Cucumis sativus) plants grown under short and long day conditions was determined. More ethylene was evolved from floral buds and apices bearing buds than from whole seedlings of comparable weight. More ethylene also was evolved from apices of the gynoecious tha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.6.998
更新日期:1972-06-01 00:00:00
abstract::Wood is an important raw material and environmentally cost-effective renewable source of energy. However, the molecular biology of wood formation (i.e. secondary growth) is surprisingly understudied. A novel experimental system was employed to study the molecular regulation of secondary xylem formation in Arabidopsis....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.038844
更新日期:2004-06-01 00:00:00
abstract::Root iron mobilization genes are induced by iron deficiency downstream of an unknown signaling mechanism. The FER gene, encoding a basic helix-loop-helix domain protein and putative transcription factor, is required for induction of iron mobilization genes in roots of tomato (Lycopersicon esculentum). To study upstrea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.054270
更新日期:2005-03-01 00:00:00
abstract::Contrasting metabolic regimes operate in Opuntia basilaris Engelm. and Bigelov, before and after precipitation. During periods of drought, atmospheric CO(2) exchange and transpiration are greatly reduced throughout the day/night cycle by stomatal closure and a highly impervious cuticle. The hypothesis is that endogeno...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.6.539
更新日期:1973-12-01 00:00:00
abstract::The analysis of substituent constants for the lipophilic and electronic factors in the auxin activity of substituted phenylacetic acids in elongation of coleoptile segments shows that these factors parallel those for the phenoxyacetic acids but assign reactivity in growth promotion to the meta position of phenylacetic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.11.1519
更新日期:1967-11-01 00:00:00
abstract::Rice (Oryza sativa) accumulates prolamins and glutelins as storage proteins. The latter storage protein is synthesized on the endoplasmic reticulum (ER) as a 57-kD proglutelin precursor, which is then processed into acidic and basic subunits in the protein storage vacuole. Three esp2 mutants, CM1787, EM44, and EM747, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010624
更新日期:2002-04-01 00:00:00
abstract::An NADPH-dependent NO2--reducing system was reconstituted in vitro using ferredoxin (Fd) NADP+ oxidoreductase (FNR), Fd, and nitrite reductase (NiR) from the green alga Chlamydomonas reinhardtii. NO2- reduction was dependent on all protein components and was operated under either aerobic or anaerobic conditions. NO2- ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.1.303
更新日期:1998-05-01 00:00:00
abstract::Purified glycopeptides derived from yeast invertase act as highly potent elicitors in suspension-cultured tomato (Lycopersicon esculentum [L.] Mill) cells, inducing ethylene biosynthesis and phenylalanine ammonia lyase half-maximally at concentrations of 1 to 5 nM. We previously demonstrated the presence of a high-aff...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.4.1659
更新日期:1996-12-01 00:00:00
abstract::Sections of sugar beet (Beta vulgaris L.) taproot were incubated in various concentrations of mannitol. At 0.4, 0.6, and 0.8 molar, the membrane electrical potential difference (E(m)) averaged about -130 millivolts; at 0.2 molar, about -90 millivolts; and at 0 molar, between -60 and -80 millivolts. Additions of 10 mil...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.4.1148
更新日期:1986-12-01 00:00:00
abstract::The water-soluble polysaccharides (SEPS) secreted into the medium by suspension-cultured sycamore cells were examined to determine whether the polysaccharides were the same as those present in the walls of sycamore cells. The SEPS were made more amenable to fractionation by treatment with a highly purified alpha-1,4-e...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.1012
更新日期:1986-04-01 00:00:00
abstract::Histone H2B monoubiquitination (H2Bub1) is an important regulatory mechanism in eukaryotic gene transcription and is essential for normal plant development. However, the function of H2Bub1 in reproductive development remains elusive. Here, we report rice (Oryza sativa) HISTONE MONOUBIQUITINATION1 (OsHUB1) and OsHUB2, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.256578
更新日期:2015-08-01 00:00:00
abstract::Rhizobium promoters involved in the formation of root nodules on leguminous plants are activated by flavonoids in plant root exudate. A series of Rhizobium strains which all contain the inducible Rhizobium leguminosarum nodA promoter fused to the Escherichia coli lacZ gene, and which differ only in the source of the r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.4.1298
更新日期:1988-04-01 00:00:00
abstract::Seed production generally requires the mating of opposite sex gametes. Apomixis, an asexual mode of reproduction, avoids both meiotic reduction and egg fertilization. The essential feature of apomixis is that an embryo is formed autonomously by parthenogenesis from an unreduced egg of an embryo sac generated through a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.062059
更新日期:2005-08-01 00:00:00
abstract::An oxidative chain reaction of sulfite initiated by the superoxide ion produced in the Mehler reaction has been implicated in the damage of plants exposed to sulfur dioxide. The toxicity of SO(2) may be alleviated by free radical scavenging systems acting to terminate this chain reaction. Hence, the relative sensitivi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.1.88
更新日期:1991-09-01 00:00:00
abstract::The intracellular distribution of enzymes capable of catalyzing the reactions from phosphoglycolate to glycerate in the bluegreen colored eucaryotic alga Cyanidium caldarium has been studied. After separating the organelles from a crude homogenate on a linear flotation gradient, the enzymes glycolate oxidase and gluta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.3.799
更新日期:1989-07-01 00:00:00
abstract::It was previously reported that the hypernodulating soybean (Glycine max [L.] Merr.) mutants, derived from the cultivar Williams, had higher root concentration of isoflavonoid compounds (daidzein, genistein, and coumestrol) than did Williams at 9 to 12 days after inoculation with Bradyrhizobium japonicum. These compou...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.4.1277
更新日期:1991-08-01 00:00:00
abstract::In sweet pea (Lathyrus odoratus L.) the mutant allele l reduced the level of gibberellin A1 (GA1) in expanding leaflets and resulted in smaller, more oval leaflets compared with the wild type. The apical portions of 6-d-old wild-type (L) seedlings also contained less GA1 and produced smaller, more oval leaflets than d...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.2.603
更新日期:1993-06-01 00:00:00
abstract::(13)C nuclear magnetic resonance spectroscopy of intact leaves of Kalanchoë tubiflora was used to observe Crassulacean acid metabolism in vivo. (13)C signals from C-4 of malate were observed after overnight exposure of leaves to (13)CO(2). Illumination of the labeled leaves resulted in a gradual decrease in the malate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.2.517
更新日期:1983-10-01 00:00:00
abstract::The temporal and spatial expression patterns of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes were investigated in pollinated orchid (Phalaenopsis spp.) flowers. Pollination signals initiate a cascade of development events in multiple floral organs, including the induction of ethylene biosynthesis, whic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.1.419
更新日期:1998-01-01 00:00:00
abstract::The phytochromes are one of the means via which plants obtain information about their immediate environment and the changing seasons. Phytochromes have important roles in developmental events such as the switch to flowering, the timing of which can be crucial for the reproductive success of the plant. Analysis of phyB...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.018135
更新日期:2003-04-01 00:00:00
abstract::The effect of temperature on respiration and kinetics of H(2)PO(4) (-) and K(+) uptake in corn roots was determined in the range of 2 to 42 C. The response of uptake to temperature, determined from Q(10) and activation energy (Ea) data, for the anion and the cation differ significantly, especially in the range of upta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.4.815
更新日期:1981-04-01 00:00:00
abstract::Callus cultures of peanut (Arachis hypogaea L. cv Valencia Tennessee Red) cotyledons grown on Linsmaier and Skoog medium containing normal levels of auxin and cytokinin do not synthesize either 4-methyl-eneglutamic acid or 4-methyleneglutamine, which nonprotein amino acids are normally found in significant amounts in ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.1.161
更新日期:1984-09-01 00:00:00
abstract::In Solanaceae, the S-specific interaction between the pistil S-RNase and the pollen S-Locus F-box protein controls self-incompatibility (SI). Although this interaction defines the specificity of the pollen rejection response, the identification of three pistil essential modifier genes unlinked to the S-locus (HT-B, 12...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01884
更新日期:2017-11-01 00:00:00
abstract::Photorespiration rates in tissue cultures of a C(4) plant, Portulaca oleracea, were compared to those in tissue cultures of a C(3) plant, Streptanthus tortuosus. The C(4) plant tissue cultures have one-half to one-third the photorespiration rate of the C(3) plant tissue cultures and respond to varying O(2) concentrati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.4.573
更新日期:1976-10-01 00:00:00
abstract::DNA polymerase zeta catalytic subunit REV3 is known to play an important role in the repair of DNA damage induced by cross-linking and methylating agents. Here, we demonstrate that in Arabidopsis (Arabidopsis thaliana), the basic polymerase activity of REV3 is essential for resistance protection against these differen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00806
更新日期:2015-12-01 00:00:00
abstract::Gene expression in the aleurone and endosperm is highly regulated during both seed development and germination. Studies of alpha-amylase expression in the aleurone of barley (Hordeum vulgare) have generated the current paradigm for hormonal control of gene expression in germinating cereal grain. Gene expression studie...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.3.929
更新日期:1994-11-01 00:00:00