Subcellular Distribution of Enzymes of Glycolate Metabolism in the Alga Cyanidium caldarium.

Abstract:

:The intracellular distribution of enzymes capable of catalyzing the reactions from phosphoglycolate to glycerate in the bluegreen colored eucaryotic alga Cyanidium caldarium has been studied. After separating the organelles from a crude homogenate on a linear flotation gradient, the enzymes glycolate oxidase and glutamate-glyoxylate aminotransferase along with catalase were present in the peroxisomal fraction (density: 1.23 grams per cubic centimeter). Serine hydroxymethyltransferase was found in the mitochondrial fraction (density: 1.18 grams per cubic centimeter). In contrast to the observations in green leaves of higher plants, the enzymes for the conversion of serine to glycerate (serine-glyoxylate aminotransferase and hydroxypyruvate reductase) were found only in the soluble fraction of the gradient. The partial characterization of enzymes from Cyanidium participating in glycolate metabolism revealed only slight differences from the corresponding enzymes from higher plants. The phylogenetic implications of the observed similarities between the enigmatic alga Cyanidium and higher plants are discussed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Gross W,Beevers H

doi

10.1104/pp.90.3.799

subject

Has Abstract

pub_date

1989-07-01 00:00:00

pages

799-805

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

90

pub_type

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