Abstract:
:1-Deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) catalyzes the first committed step of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis. In Arabidopsis, DXR is encoded by a single-copy gene. We have cloned a full-length cDNA corresponding to this gene. A comparative analysis of all plant DXR sequences known to date predicted an N-terminal transit peptide for plastids, with a conserved cleavage site, and a conserved proline-rich region at the N terminus of the mature protein, which is not present in the prokaryotic DXR homologs. We demonstrate that Arabidopsis DXR is targeted to plastids and localizes into chloroplasts of leaf cells. The presence of the proline-rich region in the mature Arabidopsis DXR was confirmed by detection with a specific antibody. A proof of the enzymatic function of this protein was obtained by complementation of an Escherichia coli mutant defective in DXR activity. The expression pattern of beta-glucuronidase, driven by the DXR promoter in Arabidopsis transgenic plants, together with the tissue distribution of DXR transcript and protein, revealed developmental and environmental regulation of the DXR gene. The expression pattern of the DXR gene parallels that of the Arabidopsis 1-deoxy-D-xylulose 5-phosphate synthase gene, but the former is slightly more restricted. These genes are expressed in most organs of the plant including roots, with higher levels in seedlings and inflorescences. The block of the 2-C-methyl-D-erythritol 4-phosphate pathway in Arabidopsis seedlings with fosmidomycin led to a rapid accumulation of DXR protein, whereas the 1-deoxy-D-xylulose 5-phosphate synthase protein level was not altered. Our results are consistent with the participation of the Arabidopsis DXR gene in the control of the 2-C-methyl-D-erythritol 4-phosphate pathway.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Carretero-Paulet L,Ahumada I,Cunillera N,Rodríguez-Concepción M,Ferrer A,Boronat A,Campos Ndoi
10.1104/pp.003798subject
Has Abstractpub_date
2002-08-01 00:00:00pages
1581-91issue
4eissn
0032-0889issn
1532-2548journal_volume
129pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Northern blot hybridization analyzes revealed that poly(A(+)) RNAs homologous to eight heat shock (HS)-specific cDNA clones were induced by arsenite (As) or Cd treatments. The mRNAs accumulated slower, and maximum accumulations were consistently lower than HS-induced levels. Prolonged treatment with low concentrations...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.4.1048
更新日期:1988-04-01 00:00:00
abstract::The water fern, Azolla caroliniana Willd., containing the symbiotic, heterocystous blue-green alga, Anabaena azollae, has been studied under various growth conditions to characterize its light-dependent production of H(2). The response of H(2) production to N(2) and C(2)H(2) and the absence of a differential effect of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.2.119
更新日期:1976-08-01 00:00:00
abstract::The enormous diversity of seed traits is an intriguing feature and critical for the overwhelming success of higher plants. In particular, seed mass is generally regarded to be key for seedling development but is mostly approximated by using scanning methods delivering only two-dimensional data, often termed seed size....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01122
更新日期:2016-11-01 00:00:00
abstract::We evaluated the symbiotic phenotypes of nodulation-restrictive and normal soybean isolines by inoculating Clark (genotypically Rj(1)Rj(1)) and mutant Clark-rj(1) (genotypically rj(1)rj(1)) seedlings in plastic growth pouches. Nodules first appeared on Clark seedlings inoculated with Bradyrhizobium japonicum USDA 94 a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.4.1291
更新日期:1987-08-01 00:00:00
abstract::The increase in proline induced by ABA, a process stimulated by NaCl or KCl in barley leaves, did not occur when Na(+) (or K(+)) was present in the external medium as the gluconate salt, namely with an anion unable to permeate the plasma membrane. However, proline increase was restored, to different extents, by the ad...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.3.927
更新日期:1988-03-01 00:00:00
abstract::An efficient negative selection procedure is crucial to the isolation of rare homologous recombinants in gene targeting. Although gene targeting is a common practice in lower eukaryotes and is becoming routine in mammals, its application to plants has not been achieved. In this report, we have evaluated an antisense c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.1.287
更新日期:1993-05-01 00:00:00
abstract::A combined system has been developed in which epidermal cell turgor, leaf water potential, and gas exchange were determined for transpiring leaves of Tradescantia virginiana L. Uniform and stable values of turgor were observed in epidermal cells (stomatal complex cells were not studied) under stable environmental cond...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.1.66
更新日期:1985-05-01 00:00:00
abstract::As a result of the phenylpropanoid pathway, many Brassicaceae produce considerable amounts of soluble hydroxycinnamate conjugates, mainly sinapate esters. From oilseed rape (Brassica napus), we cloned two orthologs of the Arabidopsis (Arabidopsis thaliana) gene reduced epidermal fluorescence1 (REF1) encoding a conifer...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.215491
更新日期:2013-04-01 00:00:00
abstract::Microsomal membranes from suspension-cultured Catharanthus roseus cells possess an enzymic activity that catalyzes the ATP-dependent phosphorylation of phosphatidic acid (PA) to form diacylglycerol pyrophosphate (H. Behrbohm, J.B. Wissing [1993] FEBS Lett 315: 95-99). This enzyme activity, PA kinase, was purified and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.4.1243
更新日期:1993-08-01 00:00:00
abstract::Arabidopsis (Arabidopsis thaliana) GOLDEN2-LIKE (GLK) transcription factors promote chloroplast biogenesis by regulating the expression of photosynthesis-related genes. Arabidopsis GLK1 is also known to participate in retrograde signaling from chloroplasts to the nucleus. To elucidate the mechanism by which GLK1 is re...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01546
更新日期:2017-01-01 00:00:00
abstract::Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the starchy endosperm protein of rice (Oryza sativa L. Japonica cv Koshihikari) during seed development confirmed that storage protein begins to accumulate about 5 days after flowering. Two polypeptide groups, 22 to 23 and 37 to 39 kilodaltons, the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.4.1094
更新日期:1982-10-01 00:00:00
abstract::The plant-specific translation initiation complex eIFiso4F is encoded by three genes in Arabidopsis (Arabidopsis thaliana)-genes encoding the cap binding protein eIFiso4E (eifiso4e) and two isoforms of the large subunit scaffolding protein eIFiso4G (i4g1 and i4g2). To quantitate phenotypic changes, a phenomics platfor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00557
更新日期:2019-09-01 00:00:00
abstract::Stomatal closing movements in response to changes from CO(2)-free to CO(2)-containing air were recorded in leaf sections of Zea mays using air flow porometers. The response to CO(2) was fast; the shortest lag between the application of 300 microliters CO(2) per liter of air and the beginning of a stomatal response was...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.2.229
更新日期:1972-02-01 00:00:00
abstract::The correct separation of homologous chromosomes during meiosis I, and sister chromatids during meiosis II, relies on the tight control of the cohesion complex. The phosphorylation and subsequent cleavage of the meiotic recombination protein REC8 (REC8-like family protein [SYN1] in Arabidopsis [Arabidopsis thaliana]),...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00281
更新日期:2018-09-01 00:00:00
abstract::After the application of 2.36 Curies per millimole [2,3-(3)H]gibberellin A(20) (GA(20)) to 21-day-old maize (Zea mays L., hybrid CM7 x CM49) plants, etiolated maize seedlings, or maturing maize cobs, a number of (3)H-metabolites were observed. The principal acidic (pH 3.0), ethyl acetate-soluble metabolite was identif...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.6.1614
更新日期:1982-12-01 00:00:00
abstract::Phosphoenolpyruvate carboxylase was purified from corn root tips about 80-fold by centrifugation, ammonium sulfate fractionation, and anion exchange and gel filtration chromatography. The resulting preparation was essentially free from malate dehydrogenase, isocitrate dehydrogenase, malate enzyme, NADH oxidase, and py...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.12.1919
更新日期:1968-12-01 00:00:00
abstract::Key developmental processes that occur on the subcellular and cellular level or occur in occluded tissues are difficult to access, let alone image and analyze. Recently, culturing living samples within polydimethylsiloxane (PDMS) microfluidic devices has facilitated the study of hard-to-reach developmental events. Her...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00879
更新日期:2016-09-01 00:00:00
abstract::As a step to understanding how the photoreceptor phytochrome acts to change the transcription of specific nuclear genes in Lemna gibba, we wish to compare promoter elements involved in negative regulation by phytochrome with those involved in positive regulation. We have isolated three genes negatively regulated by ph...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.101.3.915
更新日期:1993-03-01 00:00:00
abstract::The lipid and protein composition of the plasma membrane isolated from mulberry (Morus bombycis Koidz.) bark cells was analyzed throughout the cold acclimation period under natural and controlled environment conditions. There was a significant increase in phospholipids and unsaturation of their fatty acids during cold...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.1.257
更新日期:1984-09-01 00:00:00
abstract::The occurrence of fungicidal, elemental S is well documented in certain specialized prokaryotes, but has rarely been detected in eukaryotes. Elemental S was first identified in this laboratory as a novel phytoalexin in the xylem of resistant genotypes of Theobroma cacao, after infection by the vascular, fungal pathoge...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::We have previously shown that in Nicotiana sylvestris cytoplasmic male-sterile (CMS) mutants where the mtDNA lacks the nad7 gene coding for a subunit of respiratory Complex I (NADH:ubiquinone oxidoreductase, EC 1.6.5.3), glycine (Gly) oxidation was lower than in the wild type and insensitive to rotenone, suggesting Co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.3.1239
更新日期:2000-11-01 00:00:00
abstract::Cotyledons of light-grown soybean (Glycine max L. var Wayne) seedlings were used as a model system to study the possibility that aging requires qualitative changes in protein synthesis. Cotyledons reached a final stage of senescence and then abscised about 22 days after imbibition. Cotyledon senescence was reversed at...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.4.861
更新日期:1983-04-01 00:00:00
abstract::Evidence that indoleacetic acid (IAA) conjugates are metabolized via enzyme-catalyzed hydrolysis to free IAA and that their biological activities are related to the rates at which they are hydrolyzed by the tissue is presented. These conclusions are based on the following observations. Slow but continuous decarboxylat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.6.1424
更新日期:1981-12-01 00:00:00
abstract::A beta-primeverosidase from tea (Camellia sinensis) plants is a unique disaccharide-specific glycosidase, which hydrolyzes aroma precursors of beta-primeverosides (6-O-beta-D-xylopyranosyl-beta-D-glucopyranosides) to liberate various aroma compounds, and the enzyme is deeply concerned with the floral aroma formation i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.011023
更新日期:2002-12-01 00:00:00
abstract::An NAD-linked dehydrogenase from Chlorella pyrenoidosa Chick catalyzing the conversion of l-proline to Delta(1)-pyrroline-5-carboxylic acid was partially purified. Delta(1)-Pyrroline-5-carboxylic acid was identified as the product by co-chromatography of it and its o-aminobenzaldehyde derivative with authentic compoun...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.3.440
更新日期:1974-03-01 00:00:00
abstract::Mitochondria isolated from Kalanchoë daigremontiana, a Crassulacean acid metabolism plant, decarboxylate added malate to pyruvate at rates of up to 100 micromoles per hour per milligram original chlorophyll in the presence of ADP. Omitting ADP reduces this rate by approximately 50%. Antimycin A inhibits malate decarbo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.4.675
更新日期:1980-04-01 00:00:00
abstract::42K+ and 86Rb+ were used to determine the subcellular distribution of potassium in Chlamydomonas reinhardtii by compartmental analysis. In both wild type and a mutant strain, three distinct compartments (referred to as I, II, and III) were apparent. Using 42K+, we found that these had half-lives for K+ exchange of 1.0...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.4.1537
更新日期:1995-08-01 00:00:00
abstract::Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added. Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium. Little or no growth o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.5.598
更新日期:1970-05-01 00:00:00
abstract::Light-induced conversion of NAD to NADP was investigated in higher plants. Upon illumination, conversion of NAD to NADP was observed in intact leaves of wheat and pea following incubation in the dark. This conversion was also observed in mesophyll protoplasts of wheat leaves when they were isolated in the dark or isol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.2.324
更新日期:1981-08-01 00:00:00
abstract::We studied aquaporins in maize (Zea mays), an important crop in which numerous studies on plant water relations have been carried out. A maize cDNA, ZmTIP1, was isolated by reverse transcription-coupled PCR using conserved motifs from plant aquaporins. The derived amino acid sequence of ZmTIP1 shows 76% sequence ident...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.4.1143
更新日期:1998-08-01 00:00:00