Abstract:
:The occurrence of fungicidal, elemental S is well documented in certain specialized prokaryotes, but has rarely been detected in eukaryotes. Elemental S was first identified in this laboratory as a novel phytoalexin in the xylem of resistant genotypes of Theobroma cacao, after infection by the vascular, fungal pathogen Verticillium dahliae. In the current work, this phenomenon is demonstrated in a resistant line of tomato, Lycopersicon esculentum, in response to V. dahliae. A novel gas chromatography-mass spectroscopy method using isotope dilution analysis with 34S internal standard was developed to identify unambiguously and quantify 32S in samples of excised xylem. Accumulation of S in vascular tissue was more rapid and much greater in the disease-resistant than in the disease-susceptible line. Levels of S detected in the resistant variety (approximately 10 microg g-1 fresh weight excised xylem) were fungitoxic to V. dahliae (spore germination was inhibited >90% at approximately 3 microg mL-1). Scanning electron microscopy-energy dispersive x-ray microanalysis confirmed accumulation of S in vascular but not in pith cells and in greater amounts and frequency in the Verticillium spp.-resistant genotype. More intensive localizations of S were occasionally detected in xylem parenchyma cells, vessel walls, vascular gels, and tyloses, structures in potential contact with and linked with defense to V. dahliae. Transient increases in concentrations of sulfate, glutathione, and Cys of vascular tissues from resistant but not susceptible lines after infection may indicate a perturbation of S metabolism induced by elemental S formation; this is discussed in terms of possible S biogenesis.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Williams JS,Hall SA,Hawkesford MJ,Beale MH,Cooper RMsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
150-9issue
1eissn
0032-0889issn
1532-2548journal_volume
128pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Intact spinach and barley chloroplast normally incorporate (14)C-acetate into palmitate and oleate as the major (14)C fatty acids. Addition of nitrite markedly altered the relative patterns of the products with the appearance of stearate, a drop in oleate, but no marked change in palmitate. Arsenite greatly increased ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.4.497
更新日期:1972-04-01 00:00:00
abstract::The stamen, which consists of an anther and a filament, is the male reproductive organ in a flower. The specification of stamen identity in Arabidopsis (Arabidopsis thaliana) is controlled by a combination of the B genes APETALA3 (AP3) and PISTILLATA, the C gene AGAMOUS (AG), and the E genes SEPALLATA1 (SEP1) to SEP4....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.145896
更新日期:2009-11-01 00:00:00
abstract::Rapidly dried Tortula ruralis, a drought-tolerant moss, is known to synthesize proteins on rehydration at a much lower rate than the slowly dried moss. The reasons for this low rate of protein synthesis are unclear. We have found that during rehydration of rapidly dried moss, there is a negative correlation between th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.4.1094
更新日期:1987-12-01 00:00:00
abstract::Injection of [(14)C]tryptophan into one seed in a soybean fruit resulted in recovery of radiolabel in a fraction that cochromatographed with indoleacetic acid (IAA) through three successive high performance liquid chromatography separations. Label was found in the putative IAA fraction in all of the fruit tissues samp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.2.454
更新日期:1986-10-01 00:00:00
abstract::Syntheses are described for tetra and pentadeutero indole-3-acetic acid (IAA) labeled in positions 4, 5, 6, 7 or 2, 4, 5, 6, 7 of the indole moiety. Polydeuterated IAA is proposed as an internal standard for gas chromatographic-mass spectrometric analysis of IAA by selected ion monitoring. Nanogram amounts of IAA may ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.4.775
更新日期:1980-10-01 00:00:00
abstract::SIRK is a K(+) channel identified in grapevine (Vitis vinifera), belonging to the so-called Shaker family. The highest sequence similarities it shares with the members of this family are found with channels of the KAT type, although SIRK displays a small ankyrin domain. This atypical feature provides a key to understa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010529
更新日期:2002-02-01 00:00:00
abstract::A prominent feature of plant cells is the rapid, incessant movement of the organelles traditionally defined as cytoplasmic streaming and attributed to actomyosin motility. We sequenced six complete Nicotiana benthamiana cDNAs that encode class XI and class VIII myosins. Phylogenetic analysis indicates that these two c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.113647
更新日期:2008-03-01 00:00:00
abstract::Sucrose synthase (Sus; EC 2.4.1.13) is a key enzyme of sucrose metabolism in plant cells, providing carbon for respiration and for the synthesis of cell wall polymers and starch. Since Sus is important for plant cell growth, insights into its structure, localization, and features are useful for defining the relationsh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.115956
更新日期:2008-08-01 00:00:00
abstract::The influence of osmoticum treatments on early kernel development of maize (Zea mays L.) was studied using an in vitro culture method. Kernels with subtending cob sections were placed in culture at 5 days after pollination. Sucrose (0.29, 0.44, or 0.58 molar) and sorbitol (0, 0.15, or 0.29 molar) were used to obtain s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.3.1051
更新日期:1992-07-01 00:00:00
abstract::Effects of red and blue light at irradiances from 1.6 to 28.3 micromolar per square meter per second on chloroplast pigments, light-harvesting pigment-proteins associated with photosystem II, and the corresponding mRNA were evaluated in maize (Zea mays L.) plants (OP Golden Bantum) grown for 14 days under 14 hours lig...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.1.163
更新日期:1989-09-01 00:00:00
abstract::Isozymes of glutathione reductase (GR) have been purified from red spruce (Picea rubens Sarg.) needles. Two isozymes could be separated by anion-exchange chromatography from both nonhardened or cold-hardened tissue. Based on chromatographic elution profiles, the isozymes were designated GR-1NH and GR-2NH in preparatio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.1.205
更新日期:1994-05-01 00:00:00
abstract::The phytochrome (phy) family of sensory photoreceptors (phyA-phyE in Arabidopsis thaliana) induces changes in target-gene expression upon light-induced translocation to the nucleus, where certain members interact with selected members of the constitutively nuclear basic helix-loop-helix transcription factor family, su...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.105601
更新日期:2007-11-01 00:00:00
abstract::Photoinhibition reduces photosynthetic productivity; however, it is difficult to quantify accurately in complex canopies partly because of a lack of high-resolution structural data on plant canopy architecture, which determines complex fluctuations of light in space and time. Here, we evaluate the effects of photoinhi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00722
更新日期:2015-10-01 00:00:00
abstract::Five different regions of the first foliage leaf of etiolated barley seedlings were studied with respect to leaf growth, plastid growth and replication, differentiation of etioplasts, and conversion of etioplasts into chloroplasts upon illumination. Ultrastructural changes of the plastids were correlated with chloroph...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.2.148
更新日期:1974-08-01 00:00:00
abstract::Glycine decarboxylase (GDC) plays an important role in the photorespiratory metabolism of plants. GDC is composed of four subunits (P, H, L, and T) with the P-subunit (GLDP) serving as the actual decarboxylating unit. In C(3) plants, GDC can be found in all photosynthetic cells, whereas in leaves of C(3)-C(4) intermed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.114462
更新日期:2008-04-01 00:00:00
abstract::Previous experiments using in situ RNA hybridization have shown that the mRNAs encoding phenylalanine ammonia-lyase, 4-coumarate:coenzyme A ligase, and pathogenesis-related protein 1 accumulated transiently around fungal infection sites in parsley (Petroselinum crispum) leaf buds. These studies have now been extended ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.1.131
更新日期:1996-09-01 00:00:00
abstract::Lysine acetylation (LysAc), a form of reversible protein posttranslational modification previously known only for histone regulation in plants, is shown to be widespread in Arabidopsis (Arabidopsis thaliana). Sixty-four Lys modification sites were identified on 57 proteins, which operate in a wide variety of pathways/...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.165852
更新日期:2011-04-01 00:00:00
abstract::Changes in the physical state of microsomal membrane lipids during senescence of rose flower petals (Rosa hyb. L. cv Mercedes) were measured by x-ray diffraction analysis. During senescence of cut flowers held at 22 degrees C, lipid in the ordered, gel phase appeared in the otherwise disordered, liquid-crystalline pha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.4.1037
更新日期:1987-04-01 00:00:00
abstract::Anthocyanins are responsible for the color of many flowers, fruits, and vegetables. An interesting and unique Purple (Pr) gene mutation in cauliflower (Brassica oleracea var botrytis) confers an abnormal pattern of anthocyanin accumulation, giving the striking mutant phenotype of intense purple color in curds and a fe...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.164160
更新日期:2010-11-01 00:00:00
abstract::A water-soluble, ethanol-insoluble extract of autolytically inactive tomato (Lycopersicon esculentum Mill.) pericarp tissue contains a series of galacturonic acid-containing (pectic) oligosaccharides that will elicit a transient increase in ethylene biosynthesis when applied to pericarp discs cut from mature green fru...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.2.575
更新日期:1994-10-01 00:00:00
abstract::While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01347
更新日期:2016-12-01 00:00:00
abstract::Symbiodiniaceae are symbiotic dinoflagellates that provide photosynthetic products to corals. Because corals are distributed across a wide range of depths in the ocean, Symbiodiniaceae species must adapt to various light environments to optimize their photosynthetic performance. However, as few biochemical studies of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00726
更新日期:2020-08-01 00:00:00
abstract::Using a bioinformatics analysis of public Arabidopsis (Arabidopsis thaliana) microarray data, we propose here a novel regulatory program, combining transcriptional and posttranslational controls, which participate in modulating fluxes of amino acid metabolism in response to abiotic stresses. The program includes the f...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.115733
更新日期:2008-05-01 00:00:00
abstract::Plant species differ in response to high available manganese (Mn), but the mechanisms of sensitivity and tolerance are poorly understood. In solution culture, greater than or equal to 30 µm Mn decreased the growth of soybean (Glycine max), but white lupin (Lupinus albus), narrow-leafed lupin (Lupin angustifolius), and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00726
更新日期:2015-11-01 00:00:00
abstract::We used tissue printing and specific immunostaining to examine the localization of the alternative oxidase (AOX) protein in correlation with measurements of AOX capacity. Selected root and hypocotyl regions were analyzed during the first 14 d of growth. It is shown that AOX protein is localized in the apical meristem ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.4.1499
更新日期:1997-12-01 00:00:00
abstract::The effect of initial oxygen concentration on the rate and extent of oxidation of the respiratory chain carriers of anaerobic mitochondria from mung bean (Phaseolus aureus) seedlings was examined. The substrate was succinate, with malonate added to give malonate to succinate ratios of 6 to 12, thereby minimizing the f...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.1.95
更新日期:1972-07-01 00:00:00
abstract::The effect of vanadate on the plant plasma membrane ATPase were investigated in plasma membrane fractions derived from corn roots (Zea mays L.) and red beets (Beta vulgaris L.). The K(i) for vanadate inhibition of the plasma membrane ATPase from corn roots and red beets was between 6 and 15 micromolar vanadate. In bot...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.3.586
更新日期:1984-07-01 00:00:00
abstract::A circadian rhythm of O(2) evolution has been found in Euglena gracilis, Klebs strain Z. The rhythm persists for at least 5 days in constant dim light and temperature, but damps out in constant bright light. The phase of this rhythm can be shifted by a pulse of bright light and the period length is not changed over a ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.2.150
更新日期:1978-02-01 00:00:00
abstract::Relative elemental growth rate (REGR) profiles describe spatial patterns of growth intensity; they are indispensable for causal growth analyses. Published methods of REGR profile determination from marking experiments fall in two classes: the profile is either described by a series of segmental growth rates, or calcul...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.4.1395
更新日期:1997-04-01 00:00:00
abstract::Plasmalemma redox activity in the diatom Thalassiosira is competitively inhibited by antiserum prepared against algal nitrate reductase (NR), and fluorescent labeling experiments reveal the binding of NR antiserum to the cell surface. Furthermore, the external electron acceptor Cu bathophenanthroline disulfonate cause...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.1.143
更新日期:1988-05-01 00:00:00