Abstract:
:The effect of vanadate on the plant plasma membrane ATPase were investigated in plasma membrane fractions derived from corn roots (Zea mays L.) and red beets (Beta vulgaris L.). The K(i) for vanadate inhibition of the plasma membrane ATPase from corn roots and red beets was between 6 and 15 micromolar vanadate. In both membrane fractions, 80% to 90% of the total ATPase was inhibited at vanadate concentrations below 100 micromolar. Vanadate inhibition was optimal at pH 6.5, enhanced by the presence of K(+), and was partially reversed by 1 millimolar EDTA. The Mg:ATP kinetics for the plasma membrane ATPase were hyperbolic in both the absence and presence of vanadate. Vanadate decreased both the K(m) and V(max) of the red beet plasma membrane ATPase, indicating that vanadate inhibits the ATPase uncompetitively. These results indicate many similarities with respect to vanadate inhibition between the plant plasma membrane ATPase and other major iontranslocating ATPases from fungal and animal cells. The high sensitivity to vanadate reported here, however, differs from other reports of vanadate inhibition of the plant plasma membrane ATPase from corn, beets, and in some instances oats.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
O'neill SD,Spanswick RMdoi
10.1104/pp.75.3.586subject
Has Abstractpub_date
1984-07-01 00:00:00pages
586-91issue
3eissn
0032-0889issn
1532-2548journal_volume
75pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Diel courses of net CO2 exchange of leaves were studied in Clusia uvitana (Clusiaceae), a tropical Crassulacean acid metabolism (CAM) hemiepiphyte, growing in the crown of a 47-m tall kapok tree on Barro Colorado Island, Panama. Measurements on days without precipitation showed that net uptake of atmospheric CO2 occur...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.3.835
更新日期:1993-07-01 00:00:00
abstract::The trans-Golgi network (TGN) plays a central role in cellular secretion and has been implicated in sorting cargo destined for the plasma membrane. Previously, the Arabidopsis (Arabidopsis thaliana) echidna (ech) mutant was shown to exhibit a dwarf phenotype due to impaired cell expansion. However, ech also has a prev...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.227769
更新日期:2014-03-01 00:00:00
abstract::The control of night-break timing was studied in dark-grown seedlings of Pharbitis nil (Choisy cv. Violet) following a single continuous or skeleton photoperiod. There was a rhythmic response to a red (R) interruption of an inductive dark period, and the phasing of the rhythm was influenced by the preceding light trea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.1.277
更新日期:1982-07-01 00:00:00
abstract::Sucrose synthase (Sus; EC 2.4.1.13) is a key enzyme of sucrose metabolism in plant cells, providing carbon for respiration and for the synthesis of cell wall polymers and starch. Since Sus is important for plant cell growth, insights into its structure, localization, and features are useful for defining the relationsh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.115956
更新日期:2008-08-01 00:00:00
abstract::Phloem protein 2 (PP2) is one of the most abundant and enigmatic proteins in the phloem sap. Although thought to be associated with structural P-protein, PP2 is translocated in the assimilate stream where its lectin activity or RNA-binding properties can exert effects over long distances. Analyzing the diversity of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013086
更新日期:2003-01-01 00:00:00
abstract::Plants of Zea mays were grown with their roots confined to growing tubes, consisting of cylindrical or spherical glass tubes fitted at the bottom with a stopcock. Nutrient solution was circulated past the roots, and when a plant was 21 to 25 days old, the stopcock was closed, the root excised from the plant and connec...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.1.164
更新日期:1987-09-01 00:00:00
abstract::C(4) photosynthesis has evolved multiple times from ancestral C(3) species. Carbonic anhydrase (CA) catalyzes the reversible hydration of CO(2) and is involved in both C(3) and C(4) photosynthesis; however, its roles and the intercellular and intracellular locations of the majority of its activity differ between C(3) ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.137513
更新日期:2009-07-01 00:00:00
abstract::Bicarbonate ion, not dissolved CO(2) gas, is shown to increase 4- to 5-fold the rate of dichlorophenol indophenol reduction by isolated maize (Zea mays) chloroplasts. Glutaraldehyde fixed chloroplasts continue to exhibit bicarbonate-dependent 2,6-dichlorophenol indophenol reduction. Bicarbonate is shown to act close t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.2.119
更新日期:1973-08-01 00:00:00
abstract::Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes starch synthase IIa (SSIIa), SSIII, starch bra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.135293
更新日期:2009-03-01 00:00:00
abstract::Shifts in the duration and intensity of ambient temperature impair plant development and reproduction, particularly male gametogenesis. Stress exposure causes meiotic defects or premature spore abortion in male reproductive organs, leading to male sterility. However, little is known about the mechanisms underlying str...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00707
更新日期:2019-10-01 00:00:00
abstract::The flavoprotein (R)-(+)-mandelonitrile lyase (MDL; EC 4.1.2.10) plays a key role in cyanogenesis in rosaceous stone fruits. An MDL gene (mdl3) and its corresponding cDNA (MDL3) were isolated from black cherry (Prunus serotina) and characterized. The mdl3 gene contains 2292 bp of the 5' flanking region, the entire cod...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.4.1359
更新日期:1997-12-01 00:00:00
abstract::An NAD-linked dehydrogenase from Chlorella pyrenoidosa Chick catalyzing the conversion of l-proline to Delta(1)-pyrroline-5-carboxylic acid was partially purified. Delta(1)-Pyrroline-5-carboxylic acid was identified as the product by co-chromatography of it and its o-aminobenzaldehyde derivative with authentic compoun...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.3.440
更新日期:1974-03-01 00:00:00
abstract::Calreticulin (CRT) is a calcium-binding protein in the endoplasmic reticulum (ER) with an established role as a molecular chaper-one. An additional function in signal transduction, specifically in calcium distribution, is suggested but not proven. We have analyzed the expression pattern of Arabidopsis thaliana CRTs fo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.1.29
更新日期:1997-05-01 00:00:00
abstract::This paper reports the purification and structural determination of the mannolipid shown previously (Ericson and Delmer 1977 Plant Physiol 59: 341-347) to serve as an intermediate in glycoprotein synthesis in cotyledons of Phaseolus vulgaris. The mannolipid was purified by chromatography in organic solvents on diethyl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.1.25
更新日期:1978-01-01 00:00:00
abstract::We recently identified a cDNA encoding a putative isovaleryl-coenzyme A (CoA) dehydrogenase in Arabidopsis (AtIVD). In animals, this homotetrameric enzyme is located in mitochondria and catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. Ex...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.2.601
更新日期:2001-06-01 00:00:00
abstract::A fluorescent dye sensitive to membrane potential was used to follow the plasma-membrane potential in the unicellular halo-tolerant alga Dunaliella salina. The signal observed during dissipation of the plasma membrane potential by the addition of excess K(+) and valinomycin, or a protonophore, was taken as a measure o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.2.403
更新日期:1990-06-01 00:00:00
abstract::Superoxide dismutases (SODs) are key components of the plant antioxidant defense system. While plastidic and cytosolic isoforms have been extensively studied, the importance of mitochondrial SOD at a cellular and whole-plant level has not been established. To address this, transgenic Arabidopsis (Arabidopsis thaliana)...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.113613
更新日期:2008-05-01 00:00:00
abstract::Cells of Anabaena PCC 7119 and of a mutant strain of Nostoc muscorum unable to fix dinitrogen, grown at pH 8 and under low CO(2) tension (air), showed a reduced capacity for photosynthesis when cultured in the absence of sodium, this inhibition being followed by symptoms of photooxidation, such as chlorosis, oxygen co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.2.585
更新日期:1987-10-01 00:00:00
abstract::Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD(+)-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD(+)-d-lactat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.2.167
更新日期:1974-02-01 00:00:00
abstract::The nature of the association between nitrate reductase (NR) and membranes was examined. Nitrate reductase activity (NRA) associated with the microsomal fraction of barley (Hordeum vulgare L.) roots amounted to 0.6 to 0.8% of soluble NRA following sonication in the presence of 250 mM KI and repeated osmotic shock. T...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.3.925
更新日期:1994-01-01 00:00:00
abstract::The metabolism of labeled indole-3-acetic acid (IAA-2-(14)C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were asp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.5.666
更新日期:1976-11-01 00:00:00
abstract::The allocation and turnover of photosynthetically assimilated (14)CO(2) in lipid and protein fractions of soybean (Glycine max L. Clark) leaves and stem materials was measured. In whole plant labeling experiments, allocation of photosynthate from a pulse of (14)CO(2) into polymeric compounds was: 25% to proteins in 4 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.2.266
更新日期:1985-02-01 00:00:00
abstract::Metal accumulation in seeds is a prerequisite for germination and establishment of plants but also for micronutrient delivery to humans. To investigate metal transport processes and their interactions in seeds, we focused on METAL TOLERANCE PROTEIN8 (MTP8), a tonoplast transporter of the manganese (Mn) subclade of cat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01646
更新日期:2017-07-01 00:00:00
abstract::We have identified a wall hydrolytic enzyme from Trichoderma reesei with potent ability to induce extension of heat-inactivated type I cell walls. It is a small (23-kD) endo-1,4-beta-glucanase (Cel12A) belonging to glycoside hydrolase family 12. Extension of heat-inactivated walls from cucumber (Cucumis sativus cv Bur...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.127.1.324
更新日期:2001-09-01 00:00:00
abstract::It is demonstrated that, in etiolated pea (Pisum sativum) epicotyls, ethylene affects the activation of both monomeric GTP-binding proteins (monomeric G-proteins) and protein kinases. For monomeric G-proteins, the effect may be a rapid (2 min) and bimodal up-regulation, a transiently unimodal activation, or a transien...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.015057
更新日期:2003-04-01 00:00:00
abstract::A novel alpha-L-fucosyltransferase capable of transferring L-fucose (L-Fuc) from GDP-L-Fuc to the O-2 of alpha-L-arabinofuranosyl residue (GDP-L-Fuc:alpha-L-arabinofuranoside 2-alpha-L-fucosyltransferase) has been found in the microsomal fraction of primary roots from 6-d-old radish (Raphanus sativus L.) seedlings. En...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.2.665
更新日期:1996-02-01 00:00:00
abstract::The mechanisms of arsenic (As) hyperaccumulation in Pteris vittata, the first identified As hyperaccumulator, are unknown. We investigated the interactions of arsenate and phosphate on the uptake and distribution of As and phosphorus (P), and As speciation in P. vittata. In an 18-d hydroponic experiment with varying c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.008185
更新日期:2002-11-01 00:00:00
abstract::Uptake of Ca(2+) by suspension-cultured pear (Pyrus communis L. cv Passe Crassane) cells and protoplasts was significantly enhanced by exposure to 38 degrees C compared to 25 degrees C. The increased uptake was specific for Ca(2+) and was not due to cell wall binding. Tissues pretreated at 38 degrees C showed increase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.1.153
更新日期:1987-05-01 00:00:00
abstract::Bacterial blight is a devastating disease of rice (Oryza sativa) caused by Xanthomonas oryzae pv oryzae (Xoo). Zinc finger proteins harboring the motif with three conserved cysteine residues and one histidine residue (CCCH) belong to a large family. Although at least 67 CCCH-type zinc finger protein genes have been id...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.191379
更新日期:2012-02-01 00:00:00
abstract::Some clones of Spirodela polyrrhiza form dormant bodies called turions which require several weeks of chilling treatment before they proceed to renew growth and develop into vegetative fronds. The individual fronds of Spirodela are less than 5 mm long and can be grown aseptically in liquid culture. Turion formation an...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.11.1866
更新日期:1968-11-01 00:00:00