Effects of vanadate on the plasma membrane ATPase of red beet and corn.

Abstract:

:The effect of vanadate on the plant plasma membrane ATPase were investigated in plasma membrane fractions derived from corn roots (Zea mays L.) and red beets (Beta vulgaris L.). The K(i) for vanadate inhibition of the plasma membrane ATPase from corn roots and red beets was between 6 and 15 micromolar vanadate. In both membrane fractions, 80% to 90% of the total ATPase was inhibited at vanadate concentrations below 100 micromolar. Vanadate inhibition was optimal at pH 6.5, enhanced by the presence of K(+), and was partially reversed by 1 millimolar EDTA. The Mg:ATP kinetics for the plasma membrane ATPase were hyperbolic in both the absence and presence of vanadate. Vanadate decreased both the K(m) and V(max) of the red beet plasma membrane ATPase, indicating that vanadate inhibits the ATPase uncompetitively. These results indicate many similarities with respect to vanadate inhibition between the plant plasma membrane ATPase and other major iontranslocating ATPases from fungal and animal cells. The high sensitivity to vanadate reported here, however, differs from other reports of vanadate inhibition of the plant plasma membrane ATPase from corn, beets, and in some instances oats.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

O'neill SD,Spanswick RM

doi

10.1104/pp.75.3.586

subject

Has Abstract

pub_date

1984-07-01 00:00:00

pages

586-91

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

75

pub_type

杂志文章
  • Short-Term Regulation of Crassulacean Acid Metabolism Activity in a Tropical Hemiepiphyte, Clusia uvitana.

    abstract::Diel courses of net CO2 exchange of leaves were studied in Clusia uvitana (Clusiaceae), a tropical Crassulacean acid metabolism (CAM) hemiepiphyte, growing in the crown of a 47-m tall kapok tree on Barro Colorado Island, Panama. Measurements on days without precipitation showed that net uptake of atmospheric CO2 occur...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.3.835

    authors: Zotz G,Winter K

    更新日期:1993-07-01 00:00:00

  • ECHIDNA protein impacts on male fertility in Arabidopsis by mediating trans-Golgi network secretory trafficking during anther and pollen development.

    abstract::The trans-Golgi network (TGN) plays a central role in cellular secretion and has been implicated in sorting cargo destined for the plasma membrane. Previously, the Arabidopsis (Arabidopsis thaliana) echidna (ech) mutant was shown to exhibit a dwarf phenotype due to impaired cell expansion. However, ech also has a prev...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.227769

    authors: Fan X,Yang C,Klisch D,Ferguson A,Bhaellero RP,Niu X,Wilson ZA

    更新日期:2014-03-01 00:00:00

  • Photoperiodic Control of Flowering in Dark-Grown Seedlings of Pharbitis nil Choisy : The Effect of Skeleton and Continuous Light Photoperiods.

    abstract::The control of night-break timing was studied in dark-grown seedlings of Pharbitis nil (Choisy cv. Violet) following a single continuous or skeleton photoperiod. There was a rhythmic response to a red (R) interruption of an inductive dark period, and the phasing of the rhythm was influenced by the preceding light trea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.1.277

    authors: Lumsden P,Thomas B,Vince-Prue D

    更新日期:1982-07-01 00:00:00

  • Sucrose synthase is associated with the cell wall of tobacco pollen tubes.

    abstract::Sucrose synthase (Sus; EC 2.4.1.13) is a key enzyme of sucrose metabolism in plant cells, providing carbon for respiration and for the synthesis of cell wall polymers and starch. Since Sus is important for plant cell growth, insights into its structure, localization, and features are useful for defining the relationsh...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.115956

    authors: Persia D,Cai G,Del Casino C,Faleri C,Willemse MT,Cresti M

    更新日期:2008-08-01 00:00:00

  • Diversity of the superfamily of phloem lectins (phloem protein 2) in angiosperms.

    abstract::Phloem protein 2 (PP2) is one of the most abundant and enigmatic proteins in the phloem sap. Although thought to be associated with structural P-protein, PP2 is translocated in the assimilate stream where its lectin activity or RNA-binding properties can exert effects over long distances. Analyzing the diversity of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.013086

    authors: Dinant S,Clark AM,Zhu Y,Vilaine F,Palauqui JC,Kusiak C,Thompson GA

    更新日期:2003-01-01 00:00:00

  • Errors in the Measurement of Root Pressure and Exudation Volume Flow Rate Caused by Damage during the Transfer of Unsupported Roots between Solutions.

    abstract::Plants of Zea mays were grown with their roots confined to growing tubes, consisting of cylindrical or spherical glass tubes fitted at the bottom with a stopcock. Nutrient solution was circulated past the roots, and when a plant was 21 to 25 days old, the stopcock was closed, the root excised from the plant and connec...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.1.164

    authors: Miller DM

    更新日期:1987-09-01 00:00:00

  • Loss of the transit peptide and an increase in gene expression of an ancestral chloroplastic carbonic anhydrase were instrumental in the evolution of the cytosolic C4 carbonic anhydrase in Flaveria.

    abstract::C(4) photosynthesis has evolved multiple times from ancestral C(3) species. Carbonic anhydrase (CA) catalyzes the reversible hydration of CO(2) and is involved in both C(3) and C(4) photosynthesis; however, its roles and the intercellular and intracellular locations of the majority of its activity differ between C(3) ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.137513

    authors: Tanz SK,Tetu SG,Vella NG,Ludwig M

    更新日期:2009-07-01 00:00:00

  • Bicarbonate ion as a critical factor in photosynthetic oxygen evolution.

    abstract::Bicarbonate ion, not dissolved CO(2) gas, is shown to increase 4- to 5-fold the rate of dichlorophenol indophenol reduction by isolated maize (Zea mays) chloroplasts. Glutaraldehyde fixed chloroplasts continue to exhibit bicarbonate-dependent 2,6-dichlorophenol indophenol reduction. Bicarbonate is shown to act close t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.2.119

    authors: Stemler A,Govindjee

    更新日期:1973-08-01 00:00:00

  • Proteins from multiple metabolic pathways associate with starch biosynthetic enzymes in high molecular weight complexes: a model for regulation of carbon allocation in maize amyloplasts.

    abstract::Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes starch synthase IIa (SSIIa), SSIII, starch bra...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.135293

    authors: Hennen-Bierwagen TA,Lin Q,Grimaud F,Planchot V,Keeling PL,James MG,Myers AM

    更新日期:2009-03-01 00:00:00

  • Male Sterility in Maize after Transient Heat Stress during the Tetrad Stage of Pollen Development.

    abstract::Shifts in the duration and intensity of ambient temperature impair plant development and reproduction, particularly male gametogenesis. Stress exposure causes meiotic defects or premature spore abortion in male reproductive organs, leading to male sterility. However, little is known about the mechanisms underlying str...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00707

    authors: Begcy K,Nosenko T,Zhou LZ,Fragner L,Weckwerth W,Dresselhaus T

    更新日期:2019-10-01 00:00:00

  • Sequencing, genomic organization, and preliminary promoter analysis of a black cherry (R)-(+)-mandelonitrile lyase gene.

    abstract::The flavoprotein (R)-(+)-mandelonitrile lyase (MDL; EC 4.1.2.10) plays a key role in cyanogenesis in rosaceous stone fruits. An MDL gene (mdl3) and its corresponding cDNA (MDL3) were isolated from black cherry (Prunus serotina) and characterized. The mdl3 gene contains 2292 bp of the 5' flanking region, the entire cod...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.4.1359

    authors: Hu Z,Poulton JE

    更新日期:1997-12-01 00:00:00

  • Nicotinamide Adenine Dinucleotide-dependent Proline Dehydrogenase in Chlorella.

    abstract::An NAD-linked dehydrogenase from Chlorella pyrenoidosa Chick catalyzing the conversion of l-proline to Delta(1)-pyrroline-5-carboxylic acid was partially purified. Delta(1)-Pyrroline-5-carboxylic acid was identified as the product by co-chromatography of it and its o-aminobenzaldehyde derivative with authentic compoun...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.3.440

    authors: McNamer AD,Stewart CR

    更新日期:1974-03-01 00:00:00

  • Abundant accumulation of the calcium-binding molecular chaperone calreticulin in specific floral tissues of Arabidopsis thaliana.

    abstract::Calreticulin (CRT) is a calcium-binding protein in the endoplasmic reticulum (ER) with an established role as a molecular chaper-one. An additional function in signal transduction, specifically in calcium distribution, is suggested but not proven. We have analyzed the expression pattern of Arabidopsis thaliana CRTs fo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.1.29

    authors: Nelson DE,Glaunsinger B,Bohnert HJ

    更新日期:1997-05-01 00:00:00

  • Glycoprotein Synthesis in Plants: II. Structure of the Mannolipid Intermediate.

    abstract::This paper reports the purification and structural determination of the mannolipid shown previously (Ericson and Delmer 1977 Plant Physiol 59: 341-347) to serve as an intermediate in glycoprotein synthesis in cotyledons of Phaseolus vulgaris. The mannolipid was purified by chromatography in organic solvents on diethyl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.1.25

    authors: Delmer DP,Kulow C,Ericson MC

    更新日期:1978-01-01 00:00:00

  • The mitochondrial isovaleryl-coenzyme a dehydrogenase of arabidopsis oxidizes intermediates of leucine and valine catabolism.

    abstract::We recently identified a cDNA encoding a putative isovaleryl-coenzyme A (CoA) dehydrogenase in Arabidopsis (AtIVD). In animals, this homotetrameric enzyme is located in mitochondria and catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. Ex...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.2.601

    authors: Däschner K,Couée I,Binder S

    更新日期:2001-06-01 00:00:00

  • Plasma membrane potential of the alga dunaliella, and its relation to osmoregulation.

    abstract::A fluorescent dye sensitive to membrane potential was used to follow the plasma-membrane potential in the unicellular halo-tolerant alga Dunaliella salina. The signal observed during dissipation of the plasma membrane potential by the addition of excess K(+) and valinomycin, or a protonophore, was taken as a measure o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.403

    authors: Oren-Shamir M,Pick U,Avron M

    更新日期:1990-06-01 00:00:00

  • Decrease in manganese superoxide dismutase leads to reduced root growth and affects tricarboxylic acid cycle flux and mitochondrial redox homeostasis.

    abstract::Superoxide dismutases (SODs) are key components of the plant antioxidant defense system. While plastidic and cytosolic isoforms have been extensively studied, the importance of mitochondrial SOD at a cellular and whole-plant level has not been established. To address this, transgenic Arabidopsis (Arabidopsis thaliana)...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.113613

    authors: Morgan MJ,Lehmann M,Schwarzländer M,Baxter CJ,Sienkiewicz-Porzucek A,Williams TC,Schauer N,Fernie AR,Fricker MD,Ratcliffe RG,Sweetlove LJ,Finkemeier I

    更新日期:2008-05-01 00:00:00

  • Sodium Requirement for Photosynthesis and Its Relationship with Dinitrogen Fixation and the External CO(2) Concentration in Cyanobacteria.

    abstract::Cells of Anabaena PCC 7119 and of a mutant strain of Nostoc muscorum unable to fix dinitrogen, grown at pH 8 and under low CO(2) tension (air), showed a reduced capacity for photosynthesis when cultured in the absence of sodium, this inhibition being followed by symptoms of photooxidation, such as chlorosis, oxygen co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.2.585

    authors: Maeso ES,Piñas FF,Gonzalez MG,Valiente EF

    更新日期:1987-10-01 00:00:00

  • Enzymes related to lactate metabolism in green algae and lower land plants.

    abstract::Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD(+)-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD(+)-d-lactat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.2.167

    authors: Gruber PJ,Frederick SE,Tolbert NE

    更新日期:1974-02-01 00:00:00

  • Characterization of the association of nitrate reductase with barley (Hordeum vulgare L.) root membranes.

    abstract::The nature of the association between nitrate reductase (NR) and membranes was examined. Nitrate reductase activity (NRA) associated with the microsomal fraction of barley (Hordeum vulgare L.) roots amounted to 0.6 to 0.8% of soluble NRA following sonication in the presence of 250 mM KI and repeated osmotic shock. T...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.3.925

    authors: Meyerhoff PA,Fox TC,Travis RL,Huffaker RC

    更新日期:1994-01-01 00:00:00

  • Metabolism of Indole-3-Acetic Acid: III. Identification of Metabolites Isolated from Crown Gall Callus Tissue.

    abstract::The metabolism of labeled indole-3-acetic acid (IAA-2-(14)C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were asp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.5.666

    authors: Feung CS,Hamilton RH,Mumma RO

    更新日期:1976-11-01 00:00:00

  • Allocation and Turnover of Photosynthetically Assimilated CO(2) in Leaves of Glycine max L. Clark.

    abstract::The allocation and turnover of photosynthetically assimilated (14)CO(2) in lipid and protein fractions of soybean (Glycine max L. Clark) leaves and stem materials was measured. In whole plant labeling experiments, allocation of photosynthate from a pulse of (14)CO(2) into polymeric compounds was: 25% to proteins in 4 ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.2.266

    authors: Kagawa T,Wong JH

    更新日期:1985-02-01 00:00:00

  • Metal Tolerance Protein 8 Mediates Manganese Homeostasis and Iron Reallocation during Seed Development and Germination.

    abstract::Metal accumulation in seeds is a prerequisite for germination and establishment of plants but also for micronutrient delivery to humans. To investigate metal transport processes and their interactions in seeds, we focused on METAL TOLERANCE PROTEIN8 (MTP8), a tonoplast transporter of the manganese (Mn) subclade of cat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01646

    authors: Eroglu S,Giehl RFH,Meier B,Takahashi M,Terada Y,Ignatyev K,Andresen E,Küpper H,Peiter E,von Wirén N

    更新日期:2017-07-01 00:00:00

  • A fungal endoglucanase with plant cell wall extension activity.

    abstract::We have identified a wall hydrolytic enzyme from Trichoderma reesei with potent ability to induce extension of heat-inactivated type I cell walls. It is a small (23-kD) endo-1,4-beta-glucanase (Cel12A) belonging to glycoside hydrolase family 12. Extension of heat-inactivated walls from cucumber (Cucumis sativus cv Bur...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.127.1.324

    authors: Yuan S,Wu Y,Cosgrove DJ

    更新日期:2001-09-01 00:00:00

  • Ethylene rapidly up-regulates the activities of both monomeric GTP-binding proteins and protein kinase(s) in epicotyls of pea.

    abstract::It is demonstrated that, in etiolated pea (Pisum sativum) epicotyls, ethylene affects the activation of both monomeric GTP-binding proteins (monomeric G-proteins) and protein kinases. For monomeric G-proteins, the effect may be a rapid (2 min) and bimodal up-regulation, a transiently unimodal activation, or a transien...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.015057

    authors: Moshkov IE,Novikova GV,Mur LA,Smith AR,Hall MA

    更新日期:2003-04-01 00:00:00

  • alpha-L-fucosyltransferases from radish primary roots.

    abstract::A novel alpha-L-fucosyltransferase capable of transferring L-fucose (L-Fuc) from GDP-L-Fuc to the O-2 of alpha-L-arabinofuranosyl residue (GDP-L-Fuc:alpha-L-arabinofuranoside 2-alpha-L-fucosyltransferase) has been found in the microsomal fraction of primary roots from 6-d-old radish (Raphanus sativus L.) seedlings. En...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.2.665

    authors: Misawa H,Tsumuraya Y,Kaneko Y,Hashimoto Y

    更新日期:1996-02-01 00:00:00

  • Mechanisms of arsenic hyperaccumulation in Pteris vittata. Uptake kinetics, interactions with phosphate, and arsenic speciation.

    abstract::The mechanisms of arsenic (As) hyperaccumulation in Pteris vittata, the first identified As hyperaccumulator, are unknown. We investigated the interactions of arsenate and phosphate on the uptake and distribution of As and phosphorus (P), and As speciation in P. vittata. In an 18-d hydroponic experiment with varying c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.008185

    authors: Wang J,Zhao FJ,Meharg AA,Raab A,Feldmann J,McGrath SP

    更新日期:2002-11-01 00:00:00

  • Effect of high temperature on calcium uptake by suspension-cultured pear fruit cells.

    abstract::Uptake of Ca(2+) by suspension-cultured pear (Pyrus communis L. cv Passe Crassane) cells and protoplasts was significantly enhanced by exposure to 38 degrees C compared to 25 degrees C. The increased uptake was specific for Ca(2+) and was not due to cell wall binding. Tissues pretreated at 38 degrees C showed increase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.1.153

    authors: Klein JD,Ferguson IB

    更新日期:1987-05-01 00:00:00

  • A CCCH-type zinc finger nucleic acid-binding protein quantitatively confers resistance against rice bacterial blight disease.

    abstract::Bacterial blight is a devastating disease of rice (Oryza sativa) caused by Xanthomonas oryzae pv oryzae (Xoo). Zinc finger proteins harboring the motif with three conserved cysteine residues and one histidine residue (CCCH) belong to a large family. Although at least 67 CCCH-type zinc finger protein genes have been id...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.191379

    authors: Deng H,Liu H,Li X,Xiao J,Wang S

    更新日期:2012-02-01 00:00:00

  • Dormancy, Turion Formation, and Germination by Different Clones of Spirodela polyrrhiza.

    abstract::Some clones of Spirodela polyrrhiza form dormant bodies called turions which require several weeks of chilling treatment before they proceed to renew growth and develop into vegetative fronds. The individual fronds of Spirodela are less than 5 mm long and can be grown aseptically in liquid culture. Turion formation an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.11.1866

    authors: Perry TO

    更新日期:1968-11-01 00:00:00