Abstract:
:We have identified a wall hydrolytic enzyme from Trichoderma reesei with potent ability to induce extension of heat-inactivated type I cell walls. It is a small (23-kD) endo-1,4-beta-glucanase (Cel12A) belonging to glycoside hydrolase family 12. Extension of heat-inactivated walls from cucumber (Cucumis sativus cv Burpee Pickler) hypocotyls was induced by Cel12A after a distinct lag time and was accompanied by a large increase in wall plasticity and elasticity. Cel12A also increased the rate of stress relaxation of isolated walls at very short times (<200 ms; equivalent to reducing t(0), a parameter that estimates the minimum relaxation time). Similar changes in wall plasticity and elasticity were observed in wheat (Triticum aestivum cv Pennmore Winter) coleoptile (type II) walls, which showed only a negligible extension in response to Cel12A treatment. Thus, Cel12A modifies both type I and II walls, but substantial extension is found only in type I walls. Cel12A has strong endo-glucanase activity against xyloglucan and (1-->3,1-->4)-beta-glucan, but did not exhibit endo-xylanase, endo-mannase, or endo-galactanase activities. In terms of kinetics of action and effects on wall rheology, wall loosening by Cel12A differs qualitatively from the action by expansins, which induce wall extension by a non-hydrolytic polymer creep mechanism. The action by Cel12A mimics some of the changes in wall rheology found after auxin-induced growth. The strategy used here to identify Cel12A could be used to identify analogous plant enzymes that cause auxin-induced changes in cell wall rheology.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Yuan S,Wu Y,Cosgrove DJdoi
10.1104/pp.127.1.324subject
Has Abstractpub_date
2001-09-01 00:00:00pages
324-33issue
1eissn
0032-0889issn
1532-2548journal_volume
127pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Allene oxide synthase (AOS; hydroperoxide dehydratase; EC 4.2.1.92) catalyzes the first step in the biosynthesis of jasmonic acid from lipoxygenase-derived hydroperoxides of free fatty acids. Using the AOS cDNA from tomato (Lycopersicon esculentum), in which the role of jasmonic acid in wound-induced defense gene acti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.4.1335
更新日期:2000-04-01 00:00:00
abstract::Oleuropein, a terpene-derived glycosylated secoiridoid biosynthesized exclusively by members of the Oleaceae family, is involved in a two-component defense system comprising a β-glucosidase that activates oleuropein into a toxic glutaraldehyde-like structure. Oleuropein and its deglycosylated derivatives have high pha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00512
更新日期:2017-07-01 00:00:00
abstract::To establish infection, pathogens deliver effectors into host cells to target immune signaling components, including elements of mitogen-activated protein kinase (MPK) cascades. The virulence function of AvrRpt2, one of the first identified Pseudomonas syringae effectors, involves cleavage of the plant defense regulat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00336
更新日期:2016-07-01 00:00:00
abstract::The relative efficiency (RE) of N(2) fixation (RE = 1 - [H(2) evolved in air]/[acetylene reduced]) was investigated in a Rhizobium strain lacking uptake hydrogenase activity (Hup(-)). Variation in RE of such strains presumably reflects changes in the electron allocation coefficient of nitrogenase. By artificially exte...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.1.156
更新日期:1983-05-01 00:00:00
abstract::Diatoms constitute a major phylum of phytoplankton biodiversity in ocean water and freshwater ecosystems. They are known to respond to some chemical variations of the environment by the accumulation of triacylglycerol, but the relative changes occurring in membrane glycerolipids have not yet been studied. Our goal was...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.252395
更新日期:2015-01-01 00:00:00
abstract::Previously we showed that acid invertase activity increased and then decreased rapidly in wounded sweet potato (Ipomoea batatas Liam.) root tissue, and that the tissue contained a heat-stable, proteinaceous inhibitor with a molecular weight of about 19,500 daltons.In response to wounding of sweet potato root tissue, i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.5.879
更新日期:1977-05-01 00:00:00
abstract::Serine hydroxymethyltransferase from mammalian and bacterial sources is a pyridoxal-5'-phosphate-containing enzyme, but the requirement of pyridoxal-5'-phosphate for the activity of the enzyme from plant sources is not clear. The specific activity of serine hydroxymethyltransferase isolated from mung bean (Vigna radia...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.2.351
更新日期:1991-02-01 00:00:00
abstract::The phytochrome (phy) family of sensory photoreceptors (phyA-phyE in Arabidopsis thaliana) induces changes in target-gene expression upon light-induced translocation to the nucleus, where certain members interact with selected members of the constitutively nuclear basic helix-loop-helix transcription factor family, su...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.105601
更新日期:2007-11-01 00:00:00
abstract::Either NO(3) (-) (16 millimolar) or NH(4) (+) (1 millimolar) completely inhibited infection and nodulation of white clover seedlings (Trifoliin repens) inoculated with Rhizobium trifolii. The binding of R. trifolii to root hairs and the immunologically detectable levels of the plant lectin, trifoliin, on the root hair...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.1.18
更新日期:1978-07-01 00:00:00
abstract::The absorption characteristics of Cd(2+) by 10- to 12-day-old soybean plants (Glycine max cv Williams) were investigated with respect to influence of Cd concentration on adsorption to root surfaces, root absorption, transport kinetics and interaction with the nutrient cations Cu(2+), Fe(2+), Mn(2+), and Zn(2+). The fr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.3.844
更新日期:1983-11-01 00:00:00
abstract::A technical procedure is described to follow the in vitro release of the ;male germ unit' and the sperm cells from three tricellular pollen species (Brassica, Zea, and Triticum). The condition of the sperm cell was controlled using light microscopy. In addition, for the first time, the sperm cells viability has been c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.3.464
更新日期:1987-03-01 00:00:00
abstract::Selenium-Binding Protein1 (SBP1) gene expression was studied in Arabidopsis (Arabidopsis thaliana) seedlings challenged with several stresses, including cadmium (Cd), selenium {selenate [Se(VI)] and selenite [Se(IV)]}, copper (Cu), zinc (Zn), and hydrogen peroxide (H(2)O(2)) using transgenic lines expressing the lucif...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.144808
更新日期:2009-10-01 00:00:00
abstract::The cDNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of Arabidopsis encodes a polypeptide with an amino-terminal signal sequence for plastid import. A cDNA fragment encoding the processed form of the enzyme was expressed in Escherichia coli. The resulting protein was purified to electrophoretic homogeneit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.002626
更新日期:2002-08-01 00:00:00
abstract::It is well established that the expression of Bacillus thuringiensis (B.t.) toxin genes in higher plants is severely limited at the mRNA level, but the cause remains controversial. Elucidating whether mRNA accumulation is limited transcriptionally or posttranscriptionally could contribute to effective gene design as w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.4.1445
更新日期:1998-08-01 00:00:00
abstract::Endomembrane (endoplasmic reticulum, Golgi apparatus, plasma membrane) proteins of soybean (Glycine max) root cells are highly glycosylated. We investigated whether N-linked oligosaccharide moieties are essential for the correct intracellular transport of plant endomembrane glycoproteins. Excised roots were incubated ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.1.299
更新日期:1989-01-01 00:00:00
abstract::Relationships between net plant CO(2) exchange rate (CER) and canopy development were examined in ;jubilee' tomato over the initial 4 weeks of vegetative growth. A comparison was made between two plant groups that were alternatively exposed to 200 or 800 microeinsteins per square meter per second midday irradiation to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.3.711
更新日期:1986-03-01 00:00:00
abstract::The effects of a severe water deficit on total root (L(t)) and axial (L(x)) hydraulic conductances and on the development of the hypodermis, endodermis, and xylem were studied in sorghum (Sorghum bicolor L.). Water deficit was imposed in the upper rooting zone while the lower zones were kept moist. L(t) and L(x) were ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.1.203
更新日期:1992-05-01 00:00:00
abstract::We have developed a rapid procedure for isolating a fraction enriched in plasma membrane from Dunaliella salina using an aqueous two-phase system (dextran/polyethylene glycol, 6.7%/6.7%). An enriched plasma membrane fraction, free of chloroplast and mitochondrial contamination, could be obtained in 2.5 hours. Plasma m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.3.970
更新日期:1989-03-01 00:00:00
abstract::The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.193771
更新日期:2012-06-01 00:00:00
abstract::The interaction of tropisms is important in determining the final growth form of the plant body. In roots, gravitropism is the predominant tropistic response, but phototropism also plays a role in the oriented growth of roots in flowering plants. In blue or white light, roots exhibit negative phototropism that is medi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013847
更新日期:2003-03-01 00:00:00
abstract::Phytohemagglutinin, the lectin of the common bean Phaseolus vulgaris, has a high mannose and a modified (fucosylated) oligosaccharide on each polypeptide. Fractionation by high performance liquid chromatography of tryptic digests of [(3)H]fucose or [(3)H]glucosamine labeled phytohemagglutinin, followed by amino acid s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.1.320
更新日期:1986-05-01 00:00:00
abstract::Under stress conditions such as high light intensity or nutrient starvation, cells of the unicellular alga Dunaliella bardawil overproduce beta-carotene, which is accumulated in the plastids in newly formed triacylglycerol droplets. We report here that the formation of these sequestering structures and beta-carotene a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.4.1239
更新日期:1998-04-01 00:00:00
abstract::Glutathione is generally accepted as the principal electron donor for dehydroascorbate (DHA) reduction. Moreover, both glutathione and DHA affect cell cycle progression in plant cells. But other mechanisms for DHA reduction have been proposed. To investigate the connection between DHA and glutathione, we have evaluate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.033548
更新日期:2004-04-01 00:00:00
abstract::Arabidopsis (Arabidopsis thaliana) GARP (Golden2, ARR-B, Psr1) family transcription factors, GOLDEN2-LIKE1 and -2 (GLK1/2), function in different biological processes; however, whether and how these transcription factors modulate the response to abscisic acid (ABA) remain unknown. In this study, we used a glk1 glk2 do...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01466
更新日期:2019-04-01 00:00:00
abstract::Methionine (Met) S-methyltransferase (MMT) catalyzes the synthesis of S-methyl-Met (SMM) from Met and S-adenosyl-Met (Ado-Met). SMM can be reconverted to Met by donating a methyl group to homocysteine (homo-Cys), and concurrent operation of this reaction and that mediated by MMT sets up the SMM cycle. SMM has been hyp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.018846
更新日期:2003-04-01 00:00:00
abstract::The effect of a cold treatment on the carbohydrate status of the scales and flower stalk of Tulipa gesneriana L. cv Apeldoorn bulbs during growth after planting was studied and compared with bulbs not given cold treatment. Bulbs were stored dry for 12 weeks at 5[deg]C (precooled) or 17[deg]C (noncooled). Only the 5[de...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.2.515
更新日期:1994-02-01 00:00:00
abstract::Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses. While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata al...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00473
更新日期:2019-09-01 00:00:00
abstract::The unicellular green alga Chlorella kessleri can induce monosaccharide-H+ symport catalyzing the energy-dependent transport of D-glucose (D-Glc) and several other pentoses and hexoses across the plasmalemma. The gene coding for the inducible HUP1 monosaccharide-H+ symporter has been cloned and the protein has been ch...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.1.33
更新日期:1995-01-01 00:00:00
abstract::The Arabidopsis (Arabidopsis thaliana) genome contains four genes encoding putative NADP-malic enzymes (MEs; AtNADP-ME1-ME4). NADP-ME4 is localized to plastids, whereas the other three isoforms do not possess any predicted organellar targeting sequence and are therefore expected to be cytosolic. The plant NADP-MEs can...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.065953
更新日期:2005-09-01 00:00:00
abstract::Stem cold hardening developed to different levels in citrus types tested in controlled environments. Exotherms indicated ice spread was more uniform and rapid in unhardened than in cold-hardened stems. All attempts to inhibit the functioning of citrus leaves resulted in less cold hardening in the stems. Citrus leaves ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.4.540
更新日期:1975-10-01 00:00:00