Abstract:
:The Arabidopsis (Arabidopsis thaliana) genome contains four genes encoding putative NADP-malic enzymes (MEs; AtNADP-ME1-ME4). NADP-ME4 is localized to plastids, whereas the other three isoforms do not possess any predicted organellar targeting sequence and are therefore expected to be cytosolic. The plant NADP-MEs can be classified into four groups: groups I and II comprising cytosolic and plastidic isoforms from dicots, respectively; group III containing isoforms from monocots; and group IV composed of both monocots and dicots, including AtNADP-ME1. AtNADP-MEs contained all conserved motifs common to plant NADP-MEs and the recombinant isozymes showed different kinetic and structural properties. NADP-ME2 exhibits the highest specific activity, while NADP-ME3 and NADP-ME4 present the highest catalytic efficiency for NADP and malate, respectively. NADP-ME4 exists in equilibrium of active dimers and tetramers, while the cytosolic counterparts are present as hexamers or octamers. Characterization of T-DNA insertion mutant and promoter activity studies indicates that NADP-ME2 is responsible for the major part of NADP-ME activity in mature tissues of Arabidopsis. Whereas NADP-ME2 and -ME4 are constitutively expressed, the expression of NADP-ME1 and NADP-ME3 is restricted by both developmental and cell-specific signals. These isoforms may play specific roles at particular developmental stages of the plant rather than being involved in primary metabolism.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Wheeler MC,Tronconi MA,Drincovich MF,Andreo CS,Flügge UI,Maurino VGdoi
10.1104/pp.105.065953subject
Has Abstractpub_date
2005-09-01 00:00:00pages
39-51issue
1eissn
0032-0889issn
1532-2548pii
pp.105.065953journal_volume
139pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of the biosynthetic pathway leading to the phenolic methyl ether 3,5-dimethoxytoluene (DMT), the major scent compound of many rose (Rosa x hybrida) varieties. Modern roses are descended from both European and Chinese species, the latter being produ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.070961
更新日期:2006-01-01 00:00:00
abstract::Gas exchange parameters and stomatal physical properties were measured in Tradescantia virginiana plants grown under well-watered conditions and treated daily with either distilled water (control) or 3.0 mM abscisic acid (ABA). Photosynthetic capacity (CO(2) assimilation rate for any given leaf intercellular CO(2) con...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.2.935
更新日期:2001-02-01 00:00:00
abstract::Root nodules were harvested from chamber-grown soybean (Glycine max L. Merrill cv Woodworth) plants throughout development. Apparent nitrogenase activity (acetylene reduction) peaked before seeds began to develop, but a significant amount of activity remained as the seeds matured. Nodule senescence was defined as the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.4.797
更新日期:1983-04-01 00:00:00
abstract::Removing the pods from soybean (Glycine max [L.] Merr. cv Wye) plants induces a change in leaf function which is characterized by a change in the leaf soluble protein pattern. The synthesis of at least four polypeptides ( approximately 27, 29, 54, and 80 kilodaltons) is enhanced, and these polypeptides accumulate to l...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.1.125
更新日期:1983-09-01 00:00:00
abstract::Cobalt was shown to be essential for the symbiotic growth of Azolla filiculoides and Anabacna azollac in the absence of fixed nitrogen. Addition of 0.01 mug/liter cobalt resulted in large increases in yield, chlorophyll content and nitrogen fixation as compared to control cultures without cobalt. Cobalt was not requir...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.5.852
更新日期:1966-05-01 00:00:00
abstract::Preliminary results from differential centrifugation experiments, washing treatments, and enrichment in linear sucrose gradients at a density of 1.09 grams per cubic centimeter all indicated that beta-glucosidase activity in corn root homogenates was associated with a membrane such as tonoplast. A subsequent sucrose d...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.4.861
更新日期:1984-12-01 00:00:00
abstract::Megasporogenesis is a key step during ovule development in angiosperms, but the small number and inaccessibility of these cells have hampered molecular and genome-wide studies. Thus, many questions remain regarding the molecular basis of cell specification, differentiation, and development in the female gametophyte. H...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01254
更新日期:2020-04-01 00:00:00
abstract::A light-induced shrinkage of chloroplasts in vivo could be detected with chloroplasts isolated within 2 minutes of harvesting pea plants. As determined both by packed volume and Coulter counter, the mean volume of chloroplasts from plants in the dark was 39 mu(3), whereas it was 31 mu(3) for chloroplasts from plants i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.5.781
更新日期:1968-05-01 00:00:00
abstract::We found inconsistencies in the commonly used data for chlorophyll analysis in 80% acetone. Recently developed extinction coefficients for chlorophyll b in N,N-dimethylformamide (DMF) based on values from 80% acetone are low as a result of these inconsistencies. We determined extinction coefficients of chlorophyll a (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.2.483
更新日期:1985-02-01 00:00:00
abstract::Phototransformation of phytochrome in lettuce seeds (Lactuca sativa L. var. Grand Rapids) was examined by testing germination responses of seeds irradiated at various temperatures. Temperature variations from 0 to 50 C had no influence on the germination of partially hydrated seeds (about 15% water content) irradiated...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.2.249
更新日期:1972-08-01 00:00:00
abstract::Plant reproduction depends on the concerted activation of many genes to ensure correct communication between pollen and pistil. Here, we queried the whole transcriptome of Arabidopsis (Arabidopsis thaliana) in order to identify genes with specific reproductive functions. We used the Affymetrix ATH1 whole genome array ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.169813
更新日期:2011-04-01 00:00:00
abstract::The rates of absorption and metabolism of 3-amino-2,5-dichlorobenzoic acid (amiben) were investigated in 3-day-old roots of amiben-sensitive velvetleaf (Abutilon theophrasti Medic.) and amiben-tolerant morningglory [Ipomoea hederacea (L.) Jacq.].The initial rates of amiben absorption and binding were identically conce...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.6.854
更新日期:1969-06-01 00:00:00
abstract::A theory is presented to explain the phloem mobility of certain systemic xenobiotics that are not weak acids. It is shown that there is a theoretically optimum permeability that permits optimum circulation through the symplasm and apoplast (including the phloem and xylem) of Solanum tuberosum plants. The optimum perme...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.2.367
更新日期:1979-02-01 00:00:00
abstract::Calcium phosphate-boric acid treatments and UDP-glucose both elicited aniline blue fluorescent, periodic acid-Schiff's reagent-resistant, deposits in association with the cell walls of cowpea (Vigna sinensis [Torner] Savi cv. Early Ramshorn) tissue. Those deposits induced by calcium phosphateboric acid treatment ultra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.2.366
更新日期:1982-02-01 00:00:00
abstract::The carbon dioxide (CO2)-concentrating mechanism of cyanobacteria is characterized by the occurrence of Rubisco-containing microcompartments called carboxysomes within cells. The encapsulation of Rubisco allows for high-CO2 concentrations at the site of fixation, providing an advantage in low-CO2 environments. Cyanoba...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.237941
更新日期:2014-05-01 00:00:00
abstract::Photooxidative destruction of chloroplasts by exposure of norflurazon-treated cucumber (Cucumis sativus L.) seedlings to white light leads to reduced levels of the nuclear-encoded, peroxisomal enzyme hydroxypyruvate reductase. The partial reduction in hydroxypyruvate reductase activity under photooxidative conditions ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.2.681
更新日期:1992-06-01 00:00:00
abstract::4-Hydroxyphenylpyruvate dioxygenase (4HPPD) catalyzes the formation of homogentisate (2,5-dihydroxyphenylacetate) from p-hydroxyphenylpyruvate and molecular oxygen. In plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine. We repo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.4.1507
更新日期:1999-04-01 00:00:00
abstract::Storage protein synthesis is dependent on available nitrogen in the seed, which may be controlled by amino acid import via specific transporters. To analyze their rate-limiting role for seed protein synthesis, a Vicia faba amino acid permease, VfAAP1, has been ectopically expressed in pea (Pisum sativum) and Vicia nar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056523
更新日期:2005-04-01 00:00:00
abstract::The idea that embolized xylem vessels can be refilled while adjacent vessels remain under tension is difficult to accept if the cavitated vessels remain hydraulically connected to vessels under tension. A mechanism by which embolized conduits could be hydraulically isolated from adjacent conduits requires the existenc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.3.1015
更新日期:2000-07-01 00:00:00
abstract::We describe the development and utilization of a new imaging technology for plant biology, optical coherence microscopy (OCM), which allows true in vivo visualization of plants and plant cells. This novel technology allows the direct, in situ (e.g. plants in soil), three-dimensional visualization of cells and events i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.1.3
更新日期:2000-05-01 00:00:00
abstract::The effect of modifying calcium concentration on the expression of the photosynthesis circadian rhythm was examined in Euglena gracilis, Klebs strain Z. Expression of the oxygen evolution rhythm required the presence of both extracellular and intracellular calcium. Several treatments were found to uncouple the rate of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.1.110
更新日期:1990-05-01 00:00:00
abstract::PEX5 and PEX7 are receptors required for the import of peroxisome-bound proteins containing one of two peroxisomal targeting signals (PTS1 or PTS2). To better understand the role of PEX5 in plant peroxisomal import, we characterized the Arabidopsis (Arabidopsis thaliana) pex5-10 mutant, which has a T-DNA insertion in ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.162479
更新日期:2010-12-01 00:00:00
abstract::"Active Fe" in plants has generally been interpreted to be ferrous iron. This study evaluated some of the commonly followed active Fe determination procedures based on Fe(II) measurements. In each of 12 species examined, leaf extracts exhibited strong reducing activity and quickly reduced any added Fe(III) with or wit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.3.1017
更新日期:1990-07-01 00:00:00
abstract::Sperm cells have been isolated from pollen tubes growing in style segments of the dicotlyledon Rhododendron macgregoriae and the monocotyledon Gladiolus gandavensis by the in vivo/in vitro method at various stages of fertilization. Pollen tubes emerged from the cut end of the style into agar medium, and more than 95% ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.3.647
更新日期:1988-07-01 00:00:00
abstract::Phosphate (Pi) is one of the least available plant nutrients found in the soil. A significant amount of phosphate is bound in organic forms in the rhizosphere. Phosphatases produced by plants and microbes are presumed to convert organic phosphorus into available Pi, which is absorbed by plants. In this study we descri...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.2.728
更新日期:2001-02-01 00:00:00
abstract::Sycamore (Acer pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-dichlorophenoxyacetic acid (2,4-D) in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this ;auxin requirement,' it has been shown by gas ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.4.927
更新日期:1983-04-01 00:00:00
abstract::Eukaryotic circadian clocks utilize the ubiquitin proteasome system to precisely degrade clock proteins. In plants, the F-box-type E3 ubiquitin ligases ZEITLUPE (ZTL), FLAVIN-BINDING, KELCH REPEAT, F-BOX1 (FKF1), and LOV KELCH PROTEIN2 (LKP2) regulate clock period and couple the clock to photoperiodic flowering in res...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00331
更新日期:2018-07-01 00:00:00
abstract::Increasing concentrations of K (20, 200, 2000 mum) in the nutrient solution depressed Ca content and concentration in barley plants growing in nutrient solutions of low Ca concentrations (250 and 2500 mum). Increasing K from 20 to 200 mum depressed Ca absorption more than increasing K from 200 to 2000 mum K.The strong...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.10.1717
更新日期:1968-10-01 00:00:00
abstract::Disc electrophoresis was used to separate water soluble proteins from hardy, non-hardy, and frost killed cabbage (Brassica oleracea var. capitata) leaves. Amidoschwarz staining failed to reveal any new bands as a result of hardening although the relative amounts of proteins in individual bands changed. Sulfhydryl grou...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.2.168
更新日期:1969-02-01 00:00:00
abstract::The nature of the inorganic carbon (C(i)) species actively taken up by cyanobacteria CO(2) or HCO(3) (-) has been investigated. The kinetics of CO(2) uptake, as well as that of HCO(3) (-) uptake, indicated the involvement of a saturable process. The apparent affinity of the uptake mechanism for CO(2) was higher than t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.3.599
更新日期:1984-11-01 00:00:00