Redox regulation of Arabidopsis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.

Abstract:

:The cDNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of Arabidopsis encodes a polypeptide with an amino-terminal signal sequence for plastid import. A cDNA fragment encoding the processed form of the enzyme was expressed in Escherichia coli. The resulting protein was purified to electrophoretic homogeneity. The enzyme requires Mn(2+) and reduced thioredoxin (TRX) for activity. Spinach (Spinacia oleracea) TRX f has an apparent dissociation constant for the enzyme of about 0.2 microM. The corresponding constant for TRX m is orders of magnitude higher. In the absence of TRX, dithiothreitol partially activates the enzyme. Upon alkylation of the enzyme with iodoacetamide, the dependence on a reducing agent is lost. These results indicate that the first enzyme in the shikimate pathway of Arabidopsis appears to be regulated by the ferredoxin/TRX redox control of the chloroplast.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Entus R,Poling M,Herrmann KM

doi

10.1104/pp.002626

subject

Has Abstract

pub_date

2002-08-01 00:00:00

pages

1866-71

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

129

pub_type

杂志文章
  • Identification of 3-(O-beta-Glucosyl)-2-Indolone-3-Acetylaspartic Acid as a New Indole-3-Acetic Acid Metabolite in Vicia Seedlings.

    abstract::A new indole-3-acetic acid metabolite was isolated from broad bean (Vicia faba L. cv Chukyo) seedlings. It was a conjugate of dioxindole-3-acetic acid, aspartic acid, and glucose and was identified as 3-(O-beta-glucosyl)-2-indolone-3-acetylaspartic acid (molecular weight 484) from ultraviolet, infrared, nuclear magnet...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.667

    authors: Tsurumi S,Wada S

    更新日期:1985-11-01 00:00:00

  • Contribution of Nonautotrophic Carbon Dioxide Fixation to Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose.

    abstract::Bicarbonate-(14)C was provided to 5- and 11-day-old suspension cultures of Paul's Scarlet rose, and the incorporation of (14)C into lipid, protein, amino acids, and organic acids was determined. The rate of bicarbonate uptake was approximately the same by 5- and 11-day-old cells, but the distribution of (14)C among ce...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.4.643

    authors: Nesius KK,Fletcher JS

    更新日期:1975-04-01 00:00:00

  • Correction of Freezing Artifacts in the Application of Electron Paramagnetic Resonance for Quantification of the Reduction of Ferric Hydroxamate by Chlorella vulgaris.

    abstract::Electron paramagnetic resonance has been used to quantitate reduction of ferric ion bound to hydroxamate siderophores by iron-stressed fungi (DJ Ecker et al. 1982 J Biol Chem 257:8623-8626) and by iron-stressed Chlorella vulgaris (ATCC 11468) (FCT Allnutt, WD Bonner 1984 J Plant Nutr 7: 427-435). Application of this t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.4.739

    authors: Allnutt FC,Bonner WD

    更新日期:1987-04-01 00:00:00

  • Ozone-Induced Alterations in the Accumulation of Newly Synthesized Proteins in Leaves of Maize.

    abstract::We examined the response of leaves of 3-week-old maize (Zea mays L.) to short-term (5 h) fumigation with O3-enriched air (0, 0.12, 0.24, or 0.36 [mu]L/L). Older leaves and leaf tissue developed more severe visible damage at higher external O3 concentrations. To investigate the immediate effect of O3 exposure on the ac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.777

    authors: Pino ME,Mudd JB,Bailey-Serres J

    更新日期:1995-06-01 00:00:00

  • SnRK1 isoforms AKIN10 and AKIN11 are differentially regulated in Arabidopsis plants under phosphate starvation.

    abstract::During phosphate starvation, Snf1-related kinase 1 (SnRK1) activity significantly decreases compared with plants growing under normal nutritional conditions. An analysis of the expression of the genes encoding for the catalytic subunits of SnRK1 showed that these subunits were not affected by phosphate starvation. Tra...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.133298

    authors: Fragoso S,Espíndola L,Páez-Valencia J,Gamboa A,Camacho Y,Martínez-Barajas E,Coello P

    更新日期:2009-04-01 00:00:00

  • Lysine acetylation is a widespread protein modification for diverse proteins in Arabidopsis.

    abstract::Lysine acetylation (LysAc), a form of reversible protein posttranslational modification previously known only for histone regulation in plants, is shown to be widespread in Arabidopsis (Arabidopsis thaliana). Sixty-four Lys modification sites were identified on 57 proteins, which operate in a wide variety of pathways/...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.165852

    authors: Wu X,Oh MH,Schwarz EM,Larue CT,Sivaguru M,Imai BS,Yau PM,Ort DR,Huber SC

    更新日期:2011-04-01 00:00:00

  • Overexpression of the FAD3 desaturase gene in a mutant of Arabidopsis.

    abstract::A mutant of Arabidopsis contained increased levels of 18:3 fatty acids and correspondingly decreased levels of 18:2. The fatty acid phenotype was strongly expressed in root and seed tissues and this observation, together with other data, suggested that the mutation leads to increased activity of the endoplasmic reticu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.4.1533

    authors: Shah S,Xin Z,Browse J

    更新日期:1997-08-01 00:00:00

  • Arabidopsis FHY1 protein stability is regulated by light via phytochrome A and 26S proteasome.

    abstract::Phytochrome A (phyA) is the primary photoreceptor mediating responses to far-red light. Among the phyA downstream signaling components, Far-red Elongated Hypocotyl 1 (FHY1) is a genetically defined positive regulator of photomorphogenesis in far-red light. Both physiological and genomic characterization of the fhy1 mu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.067645

    authors: Shen Y,Feng S,Ma L,Lin R,Qu LJ,Chen Z,Wang H,Deng XW

    更新日期:2005-11-01 00:00:00

  • The isolation of spinach chloroplasts in pyrophosphate media.

    abstract::A simplified procedure (involving disruption in sorbitol-pyrophosphate mixtures) permits the separation of spinach chloroplasts which retain the ability to catalyze the photosynthetic assimilation of carbon dioxide and its associated oxygen evolution. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.9.1415

    authors: Cockburn W,Walker DA,Baldry CW

    更新日期:1968-09-01 00:00:00

  • The effect of sucrose on the differentiation of excised fern leaf tissue into either gametophytes of sporophytes.

    abstract::Excised juvenile leaves of Microgramma vacciniifolia (Polypodiaceae) develop sporophytic regenerants when grown on mineral agar with sucrose. The ratio of sporophytes to gametophytes produced from the leaf tissue increases with higher percentages of sucrose such that at 4% sucrose, the induction of aposporous gametoph...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.3.390

    authors: Hirsch AM

    更新日期:1975-09-01 00:00:00

  • Cyclopropane Fatty acids in relation to earliness in spring and drought tolerance in plants.

    abstract::Long chain cyclopropane fatty acids were observed in the sulfolipid fraction extracted from leaves of the early spring plants Galanthus nivalis L. and Anthriscus silvestris L. (Hoffm.). The content of cyclopropane fatty acids with 25 carbon atoms appeared to be clearly correlated with earliness in spring, and it range...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.3.307

    authors: Kuiper PJ,Stuiver B

    更新日期:1972-03-01 00:00:00

  • incurvata13, a novel allele of AUXIN RESISTANT6, reveals a specific role for auxin and the SCF complex in Arabidopsis embryogenesis, vascular specification, and leaf flatness.

    abstract::Auxin plays a pivotal role in plant development by modulating the activity of SCF ubiquitin ligase complexes. Here, we positionally cloned Arabidopsis (Arabidopsis thaliana) incurvata13 (icu13), a mutation that causes leaf hyponasty and reduces leaf venation pattern complexity and auxin responsiveness. We found that i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.207779

    authors: Esteve-Bruna D,Pérez-Pérez JM,Ponce MR,Micol JL

    更新日期:2013-03-01 00:00:00

  • Synthesis and uptake of cytoplasmically synthesized pyruvate, pi dikinase polypeptide by chloroplasts.

    abstract::Polyadenylated RNA was isolated from maize leaves and translated in vitro. In agreement with a previous report by others, we found among the translation products a 110-kilodalton pyruvate orthophosphate dikinase (PPDK) precursor that is about 16 kilodaltons larger than the polypeptide isolated from cells. This maize P...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.807

    authors: Aoyagi K,Bassham JA

    更新日期:1985-08-01 00:00:00

  • Characterization of ATPase activity associated with corn leaf plasma membranes.

    abstract::A Mg(2+)-dependent, cation-stimulated ATPase was associated with plasma membranes isolated from corn leaf mesophyll protoplasts. Potassium was the preferred monovalent cation for stimulating the ATPase above the Mg(2+)-activated level. The enzyme was substrate-specific for ATP, was inhibited by N,N'-dicyclohexylcarbod...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.3.521

    authors: Perlin DS,Spanswick RM

    更新日期:1981-09-01 00:00:00

  • Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterization

    abstract::A 135-kD actin-bundling protein was purified from pollen tubes of lily (Lilium longiflorum) using its affinity to F-actin. From a crude extract of the pollen tubes, this protein was coprecipitated with exogenously added F-actin and then dissociated from F-actin by treating it with high-ionic-strength solution. The pro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.4.1421

    authors: Yokota E,Shimmen KiTT

    更新日期:1998-04-01 00:00:00

  • L1 division and differentiation patterns influence shoot apical meristem maintenance.

    abstract::Plant development requires regulation of both cell division and differentiation. The class 1 KNOTTED1-like homeobox (KNOX) genes such as knotted1 (kn1) in maize (Zea mays) and SHOOTMERISTEMLESS in Arabidopsis (Arabidopsis thaliana) play a role in maintaining shoot apical meristem indeterminacy, and their misexpression...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.076075

    authors: Kessler S,Townsley B,Sinha N

    更新日期:2006-08-01 00:00:00

  • Selective delipidation of the plasma membrane by surfactants : enrichment of sterols and activation of ATPase.

    abstract::The influence of plasma membrane lipid components on the activity of the H(+)-ATPase has been studied by determining the effect of surfactants on membrane lipids and ATPase activity of oat (Avena sativa L.) root plasma membrane vesicles purified by a two-phase partitioning procedure. Triton X-100, at 25 to 1 (weight/w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.4.1524

    authors: Sandstrom RP,Cleland RE

    更新日期:1989-08-01 00:00:00

  • Specific localization of the respiratory alternative oxidase in meristematic and xylematic tissues from developing soybean roots and hypocotyls.

    abstract::We used tissue printing and specific immunostaining to examine the localization of the alternative oxidase (AOX) protein in correlation with measurements of AOX capacity. Selected root and hypocotyl regions were analyzed during the first 14 d of growth. It is shown that AOX protein is localized in the apical meristem ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.4.1499

    authors: Hilal M,Castagnaro A,Moreno H,Massa EM

    更新日期:1997-12-01 00:00:00

  • Constant Phycobilisome Size in Chromatically Adapted Cells of the Cyanobacterium Tolypothrix tenuis, and Variation in Nostoc sp.

    abstract::Phycobilisomes of Tolypothrix tenuis, a cyanobacterium capable of complete chromatic adaptation, were studied from cells grown in red and green light, and in darkness. The phycobilisome size remained constant irrespective of the light quality. The hemidiscoidal phycobilisomes had an average diameter of about 52 nanome...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.4.943

    authors: Ohki K,Gantt E,Lipschultz CA,Ernst MC

    更新日期:1985-12-01 00:00:00

  • Gibberellin biosynthesis mutations and root development in pea.

    abstract::Dwarf mutants of pea (Pisum sativum), with impaired gibberellin (GA) biosynthesis in the shoot, were studied to determine whether the roots of these genotypes had altered elongation and GA levels. Mutations na, lh-2, and ls-1 reduced GA levels in root tips and taproot elongation, although in lh-2 and ls-1 roots the re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.2.627

    authors: Yaxley JR,Ross JJ,Sherriff LJ,Reid JB

    更新日期:2001-02-01 00:00:00

  • Synthesis of chymotrypsin inhibitor I protein in potato leaflets induced by detachment.

    abstract::Chymotrypsin inhibitor I is a protein that can be induced to accumulate in potato leaflets within hr in the light when leaflets are detached from intact plants and supplied with water. This increase in inhibitor I is not accompanied by an increase in all proteins in the detached leaflets. The accumulation is polar, pr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.11.1859

    authors: Ryan CA

    更新日期:1968-11-01 00:00:00

  • Role of RNA and protein synthesis in abscission.

    abstract::The cell separation aspect of abscission is thought to involve the action of specific cell wall degrading enzymes. Enzymes represent synthesis which in turn is preceded by the synthesis of specific RNA molecules, and it follows that inhibition of either of these processes would also block abscission. Since abscission ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Abeles FB

    更新日期:1968-09-01 00:00:00

  • Defense-Related Transcript Accumulation in Phaseolus vulgaris L. Colonized by the Arbuscular Mycorrhizal Fungus Glomus intraradices Schenck Smith.

    abstract::Accumulation of mRNAs for the defense-related genes phenylalanine ammonia-lyase (PAL), chalcone synthase, chitinase (CHT), glucanase, and hydroxyproline-rich glycoprotein were examined in roots of dark red kidney bean (Phaseolus vulgaris L. cv Moncalm) colonized by the arbuscular mycorrhizal fungus Glomus intraradices...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.2.675

    authors: Blee KA,Anderson AJ

    更新日期:1996-02-01 00:00:00

  • Overproduction in Escherichia coli and Characterization of a Soybean Ferric Leghemoglobin Reductase.

    abstract::We previously cloned and sequenced a cDNA encoding soybean ferric leghemoglobin reductase (FLbR), an enzyme postulated to play an important role in maintaining leghemoglobin in a functional ferrous state in nitrogen-fixing root nodules. This cDNA was sub-cloned into an expression plasmid, pTrcHis C, and overexpressed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.1.203

    authors: Ji L,Becana M,Sarath G,Shearman L,Klucas RV

    更新日期:1994-09-01 00:00:00

  • Rhythmic Differences in the Basipetal Movement of Indoleacetic Acid between Separated Upper and Lower Halves of Geotropically Stimulated Corn Coleoptiles.

    abstract::Rhythmic fluctuation in the basipetal movement of auxin occurs in corn (Zea mays) coleoptiles oriented either in the vertical or in the horizontal position. This periodicity of transport rate varies from region to region in a horizontal coleoptile. Between an upper and lower half coleoptile (with respect to gravity), ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.2.166

    authors: Shen-Miller J

    更新日期:1973-08-01 00:00:00

  • Effect of substrate salinity on the ability for protein synthesis in pea roots.

    abstract::THE EFFECT OF SALINITY ON INCORPORATION OF AMINO ACIDS INTO ROOT TIP PROTEIN IS APPARENTLY OF DUAL NATURE: in presence of salts the uptake is depressed and the normal metabolic pathways are disturbed. If the roots were grown at high salt concentration, uptake and incorporation are affected even if they are carried out...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.7.1115

    authors: Kahane I,Poljakoff-Mayber A

    更新日期:1968-07-01 00:00:00

  • Reciprocal responses in the interaction between Arabidopsis and the cell-content-feeding chelicerate herbivore spider mite.

    abstract::Most molecular-genetic studies of plant defense responses to arthropod herbivores have focused on insects. However, plant-feeding mites are also pests of diverse plants, and mites induce different patterns of damage to plant tissues than do well-studied insects (e.g. lepidopteran larvae or aphids). The two-spotted spi...

    journal_title:Plant physiology

    pub_type: 杂志文章,meta分析

    doi:10.1104/pp.113.231555

    authors: Zhurov V,Navarro M,Bruinsma KA,Arbona V,Santamaria ME,Cazaux M,Wybouw N,Osborne EJ,Ens C,Rioja C,Vermeirssen V,Rubio-Somoza I,Krishna P,Diaz I,Schmid M,Gómez-Cadenas A,Van de Peer Y,Grbic M,Clark RM,Van Leeuwen T,

    更新日期:2014-01-01 00:00:00

  • A CCCH-type zinc finger nucleic acid-binding protein quantitatively confers resistance against rice bacterial blight disease.

    abstract::Bacterial blight is a devastating disease of rice (Oryza sativa) caused by Xanthomonas oryzae pv oryzae (Xoo). Zinc finger proteins harboring the motif with three conserved cysteine residues and one histidine residue (CCCH) belong to a large family. Although at least 67 CCCH-type zinc finger protein genes have been id...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.191379

    authors: Deng H,Liu H,Li X,Xiao J,Wang S

    更新日期:2012-02-01 00:00:00

  • Potassium Fluxes in Chlamydomonas reinhardtii (II. Compartmental Analysis).

    abstract::42K+ and 86Rb+ were used to determine the subcellular distribution of potassium in Chlamydomonas reinhardtii by compartmental analysis. In both wild type and a mutant strain, three distinct compartments (referred to as I, II, and III) were apparent. Using 42K+, we found that these had half-lives for K+ exchange of 1.0...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.4.1537

    authors: Malhotra B,Glass A

    更新日期:1995-08-01 00:00:00

  • The Arabidopsis Paf1c complex component CDC73 participates in the modification of FLOWERING LOCUS C chromatin.

    abstract::FLOWERING LOCUS C (FLC) is a key repressor of flowering in Arabidopsis (Arabidopsis thaliana) and is regulated, both positively and negatively, by posttranslational histone modifications. For example, vernalization (the promotion of flowering by cold temperatures) epigenetically silences FLC expression through repress...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.158386

    authors: Yu X,Michaels SD

    更新日期:2010-07-01 00:00:00