Abstract:
:A Mg(2+)-dependent, cation-stimulated ATPase was associated with plasma membranes isolated from corn leaf mesophyll protoplasts. Potassium was the preferred monovalent cation for stimulating the ATPase above the Mg(2+)-activated level. The enzyme was substrate-specific for ATP, was inhibited by N,N'-dicyclohexylcarbodiimide, diethylstilbestrol, p-chloromercuribenzoate, and orthovanadate, but was insensitive to oligomycin or sodium azide. A K(m) of 0.28 millimolar Mg(2+)-ATP was determined for the K(+)-ATPase, and the principal effect of potassium was on the V(max) for ATP hydrolysis. Since potassium stimulation was not saturated at high concentrations, a nonspecific role was proposed for potassium stimulation. A nonspecific phosphatase was also found to be associated with corn leaf plasma membranes. However, it could not be determined positively whether this activity represented a separate enzyme.The cation-stimulated ATPase of corn leaves is biochemically similar to other plant plasma membrane enzymes. Thus, the ATPase can serve as a reliable qualitative plasma membrane marker providing its activity is well characterized.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Perlin DS,Spanswick RMdoi
10.1104/pp.68.3.521subject
Has Abstractpub_date
1981-09-01 00:00:00pages
521-6issue
3eissn
0032-0889issn
1532-2548journal_volume
68pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Despite the fact that fungal diseases are a growing menace for conifers in modern silviculture, only a very limited number of molecular markers for pathogen resistance have been validated in conifer species. A previous genetic study indicated that the resistance of Norway spruce (Picea abies) to Heterobasidion annosum...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00685
更新日期:2016-08-01 00:00:00
abstract::Function of the cyanide-sensitive mitochondrial electron transport system was required for germination of the Zea mays embryo. Respiration of the standard electron transport system (rather than the alternate oxidase) began immediately upon initiation of imbibition. This respiration depended upon cytochrome c oxidase a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.1.295
更新日期:1990-05-01 00:00:00
abstract::The chloroplastic and cytosolic forms of spinach (Spinacia oleracea cv Long Standing Bloomsdale) leaf NADH:dihydroxyacetone phosphate (DHAP) reductase were separated and partially purified. The chloroplastic form was stimulated by dithiothreitol, reduced thioredoxin, dihydrolipoic acid, 6-phosphogluconate, and phospha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.2.379
更新日期:1988-06-01 00:00:00
abstract::Secreted slime isolated from the incubation medium of Zea mays roots maintained axenically contains fucose, arabinose, xylose, galactose, and glucose as the major monosaccharides. The slime preparation contains low levels (3% weight/weight [w/w]) of uronic acids. Methylation analysis reveals an extraordinarily diverse...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.3.771
更新日期:1986-03-01 00:00:00
abstract::Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is the precursor for the biosynthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl-CoA in the plastid is not known, but two enzymes are thought to be involved: acetyl-CoA synthetase and plastidic pyruvate dehydrogenas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.2.497
更新日期:2000-06-01 00:00:00
abstract::The succulent leaf of the obligate Crassulacean acid metabolism plant Crassula falcata comprises two distinct types of parenchyma. The peripheral tissue is dark green, whereas the central tissue is relatively colorless. We have investigated whether the conventional interpretation of Crassulacean acid metabolism-simply...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.633
更新日期:1988-11-01 00:00:00
abstract::Inactivation of inward-rectifying K+ channels (IK,in) by a rise in cytosolic free [Ca2+] ([Ca2+]i) is a key event leading to solute loss from guard cells and stomatal closure. However, [Ca2+]i action on IK,in has never been quantified, nor are its origins well understood. We used membrane voltage to manipulate [Ca2+]i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.1.277
更新日期:1999-01-01 00:00:00
abstract::An Arabidopsis (Arabidopsis thaliana) L. Heynh mutant deficient in an isoform of adenylate kinase (ADK; At2g37250) was isolated by reverse genetics. It contains a T-DNA insertion 377 bp downstream of the start point of transcription. The mutant lacks At2g37250 transcripts and has a mild reduction in total cellular ADK...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056143
更新日期:2005-01-01 00:00:00
abstract::A beta-d-glucanase highly specific for glucans containing a linkage sequence ... Glc 1 --> 4 Glc 1 --> 3 Glc 1 --> 4 Glc ... has been isolated from several commercial preparations of Bacillus subtilis alpha-amylase including one purified by repeated crystallization. The beta-d-glucanase will not hydrolyze cellulose or...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.2.300
更新日期:1977-08-01 00:00:00
abstract::N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.2.442
更新日期:1984-06-01 00:00:00
abstract::The incorporation of (14)C-2-mevalonic acid into transfer RNA and ribosomal RNA (high molecular weight RNA) in rapidly growing, cytokinin-dependent tobacco (Nicotiana tabacum var. Wisconsin No. 38) callus cultures has been investigated. Approximately 40% of the label incorporated into transfer RNA was present in a rib...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.5.853
更新日期:1975-05-01 00:00:00
abstract::The resistive and reactive components of electrical impedance were measured for birdsfoot trefoil (Lotus corniculatus L.) stems at freezing temperatures to -8 degrees C. As temperature decreased the specific resistance at frequencies between 49 hertz and 1.11 megahertz of stems from cold acclimated plants increased mo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.1.275
更新日期:1988-01-01 00:00:00
abstract::Two benzoyl-l-tyrosine p-nitroanilide hydrolases (BTPAases I and II) were purified from the etiolated leaves of Zea mays L. and characterized. BTPAase I was electrophoretically homogeneous and consisted of two identical subunits having a molecular weight of 53,000. The molecular weight of BTPAase II was 65,000. The Mi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.3.770
更新日期:1987-07-01 00:00:00
abstract::Phenol inhibits the Hill reaction with several Hill oxidants and the accompanying non-cyclic phosphorylation. It inhibits also pseudocyclic and cyclic phosphorylation. Partial reactions which are dependent on cyclic electron flow are inhibited too, but electron flow from ascorbate via dichlorophenolindophenol to TPN i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.4.573
更新日期:1967-04-01 00:00:00
abstract::The incorporation of [(14)C]phenylalanine and [1,6-(14)C]shikimic acid into tyrosine was investigated in the callus of Portulaca grandiflora, var. JR (L.). By inhibiting phenylalanine with 1-alpha-aminooxy-beta-phenyl-propionic acid and tyrosinase with 1-cysteine-HCl and hydrazine-hydrate, the possible synthesis of ty...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.2.272
更新日期:1981-08-01 00:00:00
abstract::Changes in the galactose content of the noncellulosic polysaccharides of tomato (Mill) fruit cell walls were analyzed under various conditions. On the plant, galactan decreased gradually during fruit growth. As normal fruits ripened, the loss of galactan increased sharply; this was not observed in attached rin fruits ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.3.532
更新日期:1980-09-01 00:00:00
abstract::Aluminum (Al) and proton (H⁺) tolerances are essential traits for plants to adapt to acid soil environments. In Arabidopsis (Arabidopsis thaliana), these tolerances are mediated by a zinc-finger transcription factor, SENSITIVE TO PROTON RHIZOTOXICITY1 (AtSTOP1), which regulates the transcription of multiple genes crit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.218958
更新日期:2013-08-01 00:00:00
abstract::Activity of the enzyme ADPglucose pyrophosphorylase is known to be reduced in maize (Zea mays L.) endosperm mutants at two independent loci, Shrunken-2 (Sh(2)) and Brittle-2 (Bt(2)). Spinach leaf ADPglucose pyrophosphorylase has previously been shown to comprise two subunits of 51 and 54 kilodaltons. Anti-bodies raise...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.4.881
更新日期:1990-04-01 00:00:00
abstract::While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01347
更新日期:2016-12-01 00:00:00
abstract::Chloroplast division is driven by a macromolecular complex containing components that are positioned on the cytosolic surface of the outer envelope, the stromal surface of the inner envelope, and in the intermembrane space. The only constituents of the division apparatus identified thus far are the tubulin-like protei...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::A method for isolating guard cell protoplasts (GCP) from mechanically prepared epidermis of Vicia faba is described. Epidermis was prepared by homogenizing leaves in a Waring blender in a solution of 10% Ficoll, 5 millimolar CaCl(2), and 0.1% polyvinylpyrrolidone 40 (PVP). Attached mesophyll and epidermal cells were r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.4.1382
更新日期:1989-08-01 00:00:00
abstract::The relative effects of water stress on growth parameters of subterranean clover (Trifolium subterraneum L. cv. Woogenellup) dependent on either N(2) or 8 millimolar NH(4)NO(3) for N were examined. Whole-plant carbon exchange rate (CER), acetylene reduction (AR), dry matter production, and Kjeldahl N accumulation were...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.2.416
更新日期:1982-02-01 00:00:00
abstract::Tumor formation in Vitis species and hybrids, incited by Agrobacterium tumefaciens, was altered by chemical, physical, developmental, and genetic variables. Knowledge of the effect of these variables was used to develop a stringent in vitro assay system to select parents for a study of genetic factors that modulate tu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.1.121
更新日期:1991-05-01 00:00:00
abstract::Nitrate reductase (NR) is the first enzyme in nitrate assimilation, a critical process for plant survival. The regulation of NR gene expression is complex, involving both internal and external factors. Of these, nitrate induction of NR gene expression has been studied most extensively and is well conserved among bacte...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.2.477
更新日期:1994-10-01 00:00:00
abstract::To our knowledge, this report describes the first application of video imaging of Chl fluorescence to the study of light utilization in photosystem II of attached leaves of Phaseolus vulgaris infected with the obligate biotrophic fungus Uromyces appendiculatus (race 38). The video-based detection system produced a spa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.163
更新日期:1995-05-01 00:00:00
abstract::Though Helminthosporium maydis race T (HmT) toxin decreased active Ca(2+) uptake into mitochondria isolated from susceptible (T) but not resistant (N) corn (Kimber, Sze, 1984 Plant Physiol 74: 804-809 the mode of toxin action is not understood. This study shows that HmT toxin or A23187 (a Ca(2+) ionophore) dissipated ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.1.235
更新日期:1984-05-01 00:00:00
abstract::The relationship among physiological, injury, growth, and yield responses was examined in field-grown green pepper (Capsicum annuum L. ;California Wonder') subjected to two airborne environmental stresses. The primary objectives were to determine if the stresses could cause alterations in the plant responses, and to d...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.2.477
更新日期:1988-10-01 00:00:00
abstract::Crude plasma membranes of corn (Zea mays L.) roots were obtained according to MI De Michelis and RM Spanswick (1986 Plant Physiol 81: 542-547). This preparation, which contained tightly sealed vesicles displaying Mg-ATP dependent H(+)-transport, was purified by phase partitioning. The percentage of inside-out vesicles...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.2.491
更新日期:1988-06-01 00:00:00
abstract::Three forms of pyrophosphate fructose-6-phosphate 1-phosphotransferase (PFP) were purified from both green and red tomato (Lycopersicon esculentum) fruit: (a) a classical form (designated Q(2)) containing alpha- (66 kilodalton) and beta- (60 kilodalton) subunits; (b) a form (Q(1)) containing a beta-doublet subunit; an...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.2.499
更新日期:1990-10-01 00:00:00
abstract::The nucleotide-binding site (NBS)-Leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes, and is one of the largest gene families in plant genomes. We have identified 333 nonredundant NBS-LRRs in the current Medicago truncatula draft genome (Mt1.0), likely representing ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.104588
更新日期:2008-01-01 00:00:00