Identification and characterization of nucleotide-binding site-leucine-rich repeat genes in the model plant Medicago truncatula.

abstract：:DNA coding for the enzymatically active subunit A of diphtheria toxin was placed under the control of the cauliflower mosaic virus 35S promoter and the Agrobacterium left transfer-DNA gene 7 polyadenylation signal. Agrobacteria carrying a binary plant vector with the chimeric diphtheria toxin A gene had very low trans...

journal_title：Plant physiology

authors： Czako M,An G

更新日期：1991-03-01 00:00:00

abstract：:Ribulose 1,5-diphosphate carboxylase (RUDPcase) from autotrophically grown Euglena gracilis was purified to homogeneity as measured by analytical ultracentrifugation, polyacrylamide gel electrophoresis, and immunoprecipitation reactions. The enzyme represented about 9% of total protein and 24% of soluble protein in th...

journal_title：Plant physiology

authors： Rabinowitz H,Reisfeld A,Sagher D,Edelman M

更新日期：1975-09-01 00:00:00

abstract：:Plants accumulate the lipids phosphatidic acid (PA), diacylglycerol (DAG), and triacylglycerol (TAG) during cold stress, but how plants balance the levels of these lipids to mediate cold responses remains unknown. The enzymes ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE (DGAT) and DIACYLGLYCEROL KINASE (DGK) catalyz...

journal_title：Plant physiology

authors： Tan WJ,Yang YC,Zhou Y,Huang LP,Xu L,Chen QF,Yu LJ,Xiao S

更新日期：2018-07-01 00:00:00

abstract：:The enzyme 3'-AMP nucleotidase was purified 2,500- to 5,000-fold from extracts of an acetone powder of wheat (Triticum aestivum) embryonic axes germinated for 40 hours. Sodium dodecyl sulfate acrylamide gel electrophoresis and chromatography on Biogel-P100 indicate that the enzyme is monomeric with a molecular weight ...

journal_title：Plant physiology

authors： Zhu ZP,Marsh L,Marcus A

更新日期：1983-02-01 00:00:00

abstract：:Single-cell RNA sequencing (scRNA-seq) has been used extensively to study cell-specific gene expression in animals, but it has not been widely applied to plants. Here, we describe the use of a commercially available droplet-based microfluidics platform for high-throughput scRNA-seq to obtain single-cell transcriptomes...

journal_title：Plant physiology

authors： Ryu KH,Huang L,Kang HM,Schiefelbein J

更新日期：2019-04-01 00:00:00

abstract：:Excised roots of the tomato species, Lycopersicon esculentum Mill. cv Walter (the commercial species) and of Lycopersicon cheesmanii ssp. minor (Hook.) C.H. Mull. (a wild species from the Galapagos Islands), were used in comparative studies of their absorption of K(+) and Na(+). Uptake of (86)Rb-labeled K(+) and (22)N...

journal_title：Plant physiology

authors： Wrona AF,Epstein E

更新日期：1985-12-01 00:00:00

abstract：:The surface tension (γ) of xylem sap plays a key role in stabilizing air-water interfaces at the pits between water- and gas-filled conduits to avoid air seeding at low water potentials. We studied seasonal changes in xylem sap γ in Picea abies and Pinus mugo growing at the alpine timberline. We analyzed their vulnera...

journal_title：Plant physiology

authors： Losso A,Beikircher B,Dämon B,Kikuta S,Schmid P,Mayr S

更新日期：2017-11-01 00:00:00

abstract：:We conducted a comprehensive metabolic phenotyping of potato (Solanum tuberosum L. cv Desiree) tuber tissue that had been modified either by transgenesis or exposure to different environmental conditions using a recently developed gas chromatography-mass spectrometry profiling protocol. Applying this technique, we wer...

journal_title：Plant physiology

authors： Roessner U,Willmitzer L,Fernie AR

更新日期：2001-11-01 00:00:00

abstract：:Winter cultivars of rye (Secale cereale L., cv Musketeer) and wheat (Triticum aestivum L. cvs Kharkov and Monopol), but not a spring cultivar of wheat (Glenlea), grown at cold-hardening temperatures showed, at high irradiances, a higher proportion of oxidized to reduced primary, stable quinone receptor (QA) than did t...

journal_title：Plant physiology

authors： Oquist G,Hurry VM,Huner N

更新日期：1993-01-01 00:00:00

abstract：:Gas exchange and plant luminescence (delayed light emission) of a single red kidney bean leaf undergoing synchronous oscillations in gas exchange were recorded and analyzed. Introduction of 1.1 microliter per liter SO(2) during these oscillations produced increases in plant luminescence that, when averaged over a port...

journal_title：Plant physiology

authors： Ellenson JL

更新日期：1985-08-01 00:00:00

abstract：:Calcium flux in sunflower (Helianthus annuus L. cv Russian mammoth) hypocotyl was measured with a Ca(2+) electrode as the increase or decrease in Ca(2+) in an aqueous solution (10 micromolar CaCl(2)) in contact with either the basal or apical end of 20 millimeter segments. Ca(2+) efflux was significantly higher at the...

journal_title：Plant physiology

authors： de Guzman CC,Dela Fuente RK

更新日期：1986-06-01 00:00:00

abstract：:Norway spruce (Picea abies) forests suffer periodic fatal attacks by the bark beetle Ips typographus and its fungal associate, Ceratocystis polonica. Norway spruce protects itself against fungal and bark beetle invasion by the production of terpenoid resins, but it is unclear whether resins or other defenses are effec...

journal_title：Plant physiology

authors： Hammerbacher A,Schmidt A,Wadke N,Wright LP,Schneider B,Bohlmann J,Brand WA,Fenning TM,Gershenzon J,Paetz C

更新日期：2013-07-01 00:00:00

abstract：:Nucleotide metabolism in potato (Solanum tuberosum) mitochondria was studied using 31P-nuclear magnetic resonance spectroscopy and the O2 electrode. Immediately following the addition of ADP, ATP synthesis exceeded the rate of oxidative phosphorylation, fueled by succinate oxidation, due to mitochondrial adenylate kin...

journal_title：Plant physiology

authors： Roberts J,Aubert S,Gout E,Bligny R,Douce R

更新日期：1997-01-01 00:00:00

abstract：:Chloroplast thylakoid protein phosphatase activity was measured using (32)P-labeled histone as an exogenous substrate and an assay of the (32)Pi released involving formation of a phosphomolybdate complex and organic extraction. The activity was liberated from wheat (Triticum aestivum) thylakoids by washing the membran...

journal_title：Plant physiology

authors： Sun G,Bailey D,Jones MW,Markwell J

更新日期：1989-01-01 00:00:00

abstract：:Movement of [(14)C]kinetin and [(14)C]gibberellic acid was examined in cotton (Gossypium hirsutum L.) cotyledonary petiole sections independent of label uptake or exit from the tissue. Sections 20 millimeters in length were taken from well watered, stressed, and poststressed plants. Transport capacity was determined u...

journal_title：Plant physiology

authors： Davenport TL,Jordan WR,Morgan PW

更新日期：1979-01-01 00:00:00

abstract：:Legume-rhizobia symbiosis is a time-limited process due to the onset of senescence, which results in the degradation of host plant cells and symbiosomes. A number of transcription factors, proteases, and functional genes have been associated with nodule senescence; however, whether other proteases or transcription fac...

journal_title：Plant physiology

authors： Deng J,Zhu F,Liu J,Zhao Y,Wen J,Wang T,Dong J

更新日期：2019-12-01 00:00:00

abstract：:The chloroplast response in the green alga Dunaliella salina to irradiance stress was investigated. Cells were grown under low light (LL) at 100 [mu]mol photons m-2 s-1 or high light (HL) at 2000 [mu]mol photons m-2 s-1 incident intensity. LL-grown cells had a low chlorophyll (Chl) a/b ratio, an abundance of light-har...

journal_title：Plant physiology

authors： Webb MR,Melis A

更新日期：1995-03-01 00:00:00

abstract：:Experiments were conducted with segments of corn roots to investigate whether nitrate reductase (NR) is compartmentalized in particular groups of cells that collectively form the root symplastic pathway. A microsurgical technique was used to separate cells of the epidermis, of the cortex, and of the stele. The presenc...

journal_title：Plant physiology

authors： Rufty TW,Thomas JF,Remmler JL,Campbell WH,Volk RJ

更新日期：1986-11-01 00:00:00

abstract：:The cytokinin activity has been isolated and identified from extracts of immature mango (Mangifera indica L.) seeds. The structures of zeatin, zeatin riboside, and N(6)-(Delta(2)-isopentenyl)adenine riboside were confirmed on the basis of their chromatographic behavior and mass spectra of trimethylsilyl derivatives. B...

journal_title：Plant physiology

authors： Chen WS

更新日期：1983-02-01 00:00:00

abstract：:Glucose modulates plant metabolism, growth, and development. In Arabidopsis (Arabidopsis thaliana), Hexokinase1 (HXK1) is a glucose sensor that may trigger abscisic acid (ABA) synthesis and sensitivity to mediate glucose-induced inhibition of seedling development. Here, we show that the intensity of short-term respons...

journal_title：Plant physiology

authors： Matiolli CC,Tomaz JP,Duarte GT,Prado FM,Del Bem LE,Silveira AB,Gauer L,Corrêa LG,Drumond RD,Viana AJ,Di Mascio P,Meyer C,Vincentz M

更新日期：2011-10-01 00:00:00

abstract：:We report here a method for the isolation of high specific activity phosphoglycerate kinase (EC 2.7.2.3) from chloroplasts. The enzyme has been purified over 200-fold from pea (Pisum sativum L.) stromal extracts to apparent homogeneity with 23% recovery. Negative cooperativity is observed with the two enzyme phosphogl...

journal_title：Plant physiology

authors： Macioszek J,Anderson JB,Anderson LE

更新日期：1990-09-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase was purified from corn root tips about 80-fold by centrifugation, ammonium sulfate fractionation, and anion exchange and gel filtration chromatography. The resulting preparation was essentially free from malate dehydrogenase, isocitrate dehydrogenase, malate enzyme, NADH oxidase, and py...

journal_title：Plant physiology

authors： Ting IP

更新日期：1968-12-01 00:00:00

abstract：:Control of flowering and the regulation of plant architecture have been thoroughly investigated in a number of well-studied dicot plants such as Arabidopsis, Antirrhinum, and tobacco. However, in many important monocot seed crops, molecular information on plant reproduction is still limited. To investigate the regulat...

journal_title：Plant physiology

authors： Jensen CS,Salchert K,Nielsen KK

更新日期：2001-03-01 00:00:00

abstract：:Extracts of light-grown Cucumis sativus L. seedlings catalyzed the oxidation of indole-3-acetaldehyde to indole-3-acetic acid. No added cofactors were required. Inhibitor studies indicated that the enzyme is a metalloflavoprotein. While indole-3-aldehyde, benzaldehyde, and phenylacetaldehyde partially inhibited the ox...

journal_title：Plant physiology

authors： Bower PJ,Brown HM,Purves WK

更新日期：1978-01-01 00:00:00

abstract：:Drought stress is one of the main environmental problems encountered by crop growers. Reduction in arable land area and reduced water availability make it paramount to identify and develop strategies to allow crops to be more resilient in water-limiting environments. The plant hormone abscisic acid (ABA) plays an impo...

journal_title：Plant physiology

authors： Brugière N,Zhang W,Xu Q,Scolaro EJ,Lu C,Kahsay RY,Kise R,Trecker L,Williams RW,Hakimi S,Niu X,Lafitte R,Habben JE

更新日期：2017-11-01 00:00:00

abstract：:We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogena...

journal_title：Plant physiology

authors： Zhang C,Barthelson RA,Lambert GM,Galbraith DW

更新日期：2008-05-01 00:00:00

abstract：:Short-term ion uptake into roots of Limnobium stoloniferum was followed extracellularly with ion selective macroelectrodes. Cytosolic or vacuolar pH, together with the electrical membrane potential, was recorded with microelectrodes both located in the same young root hair. At the onset of chloride, phosphate, and nit...

journal_title：Plant physiology

authors： Ullrich CI,Novacky AJ

更新日期：1990-12-01 00:00:00

abstract：:Six Arabidopsis (Arabidopsis thaliana) clade A protein phosphatase 2Cs (PP2Cs) have established abscisic acid (ABA) signaling roles; however, phenotypic roles of the remaining three "HAI" PP2Cs, Highly ABA-Induced1 (HAI1), AKT1-Interacting PP2C1/HAI2, and HAI3, have remained unclear. HAI PP2C mutants had enhanced prol...

journal_title：Plant physiology

authors： Bhaskara GB,Nguyen TT,Verslues PE

更新日期：2012-09-01 00:00:00

abstract：:The effect of external CO2 concentration on the expression of carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was examined in pea (Pisum sativum cv Little Marvel) leaves. Enzyme activities and their transcript levels were reduced in plants grown at 1000 [mu]L/L CO2 compared with p...

journal_title：Plant physiology

authors： Majeau N,Coleman JR

更新日期：1996-10-01 00:00:00

abstract：:Upon Agrobacterium tumefaciens infection of a host plant, Tumor morphology root (Tmr) a bacterial adenosine phosphate-isopentenyltransferase (IPT), creates a metabolic bypass in the plastid for direct synthesis of trans-zeatin (tZ) with 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate as the prenyl donor. To understand ...

journal_title：Plant physiology

authors： Ueda N,Kojima M,Suzuki K,Sakakibara H

更新日期：2012-07-01 00:00:00