Histochemical Compartmentation of Photosynthesis in the Crassulacean Acid Metabolism Plant Crassula falcata.

Abstract:

:The succulent leaf of the obligate Crassulacean acid metabolism plant Crassula falcata comprises two distinct types of parenchyma. The peripheral tissue is dark green, whereas the central tissue is relatively colorless. We have investigated whether the conventional interpretation of Crassulacean acid metabolism-simply, temporal separation of light and dark CO(2) fixation within individual cells-is sufficient. Ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) and chlorophyll, indicating the photosynthetic-carbon-reduction pathway, were concentrated in peripheral tissue. Specific activities of P-enolpyruvate carboxylase (4.1.1.31) and of NAD(+)-malic enzyme (1.1.1.39), indicating capacity for dark CO(2) fixation and release, respectively, were high in both types of parenchyma. Measured directly as malic acid decline at the beginning of the photoperiod, CO(2) "storage" occurred in both tissues. These data indicate that there is a spatial component to Crassulacean acid metabolism in C. falcata.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Springer SA,Outlaw WH

doi

10.1104/pp.88.3.633

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

633-8

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

88

pub_type

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