Overexpression of the FAD3 desaturase gene in a mutant of Arabidopsis.

Abstract:

:A mutant of Arabidopsis contained increased levels of 18:3 fatty acids and correspondingly decreased levels of 18:2. The fatty acid phenotype was strongly expressed in root and seed tissues and this observation, together with other data, suggested that the mutation leads to increased activity of the endoplasmic reticulum 18:2 desaturase encoded by the FAD3 gene. Gel-blot analysis of RNA from wild-type and mutant plants established that FAD3 transcript levels were increased 80% in the mutant relative to the wild type. Genetic analysis demonstrated a linkage between the new mutation and the fad3 locus. Linkage of the mutation to fad3 raises the possibility that the lesion is an alteration to the promoter or another regulatory region of the FAD3 gene, which results in increased transcription.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Shah S,Xin Z,Browse J

doi

10.1104/pp.114.4.1533

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

1533-9

issue

4

eissn

0032-0889

issn

1532-2548

pii

114/4/1533

journal_volume

114

pub_type

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