Abstract:
:A mutant of Arabidopsis contained increased levels of 18:3 fatty acids and correspondingly decreased levels of 18:2. The fatty acid phenotype was strongly expressed in root and seed tissues and this observation, together with other data, suggested that the mutation leads to increased activity of the endoplasmic reticulum 18:2 desaturase encoded by the FAD3 gene. Gel-blot analysis of RNA from wild-type and mutant plants established that FAD3 transcript levels were increased 80% in the mutant relative to the wild type. Genetic analysis demonstrated a linkage between the new mutation and the fad3 locus. Linkage of the mutation to fad3 raises the possibility that the lesion is an alteration to the promoter or another regulatory region of the FAD3 gene, which results in increased transcription.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Shah S,Xin Z,Browse Jdoi
10.1104/pp.114.4.1533subject
Has Abstractpub_date
1997-08-01 00:00:00pages
1533-9issue
4eissn
0032-0889issn
1532-2548pii
114/4/1533journal_volume
114pub_type
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