Biosynthesis of cytidine diphosphate diglyceride by enzyme preparations from cauliflower.

Abstract:

:The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme has a pH optimum of 5.6, an optimal Mn(2+) concentration of 1mm and an optimal Mg(2+) concentration of 30 mm. At the optimal concentration, Mn(2+) was 4 times better than Mg(2+). The utilization of biosynthesized cytidine diphosphate diglyceride can be enhanced by addition of inositol, thereby causing the formation of phosphatidyl inositol.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Sumida S,Mudd JB

doi

10.1104/pp.45.6.719

subject

Has Abstract

pub_date

1970-06-01 00:00:00

pages

719-22

issue

6

eissn

0032-0889

issn

1532-2548

journal_volume

45

pub_type

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