Abstract:
:Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersicon esculentum) plants. The two new members, P69E and P69F, are organized in a cluster and arranged in a tandem form. mRNA expression analysis and studies of transgenic Arabidopsis plants transformed with promoter-beta-glucuronidase fusions for each of these two genes revealed that they are differentially regulated, with each showing a highly specific mRNA expression pattern. P69E mRNA is expressed only in roots, while P69F mRNA is expressed only in hydathodes. A comparison of all the P69 amino acid sequences, gene structure, expression profiles, and clustered organization suggests a working model for P69 gene family evolution.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Jordá L,Conejero V,Vera Pdoi
10.1104/pp.122.1.67subject
Has Abstractpub_date
2000-01-01 00:00:00pages
67-74issue
1eissn
0032-0889issn
1532-2548journal_volume
122pub_type
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