Properties of Ornithine Carbamoyltransferase from Pisum sativum L.

Abstract:

:Some properties of ornithine carbamoyltransferase from chloroplasts isolated from leaves of Pisum sativum L. (cv Marzia) were compared with those of the enzyme partially purified (316-fold) from shoots of seedlings after 3 weeks of cultivation.Both preparations showed a pH optimum at pH 8.3 and had the same affinity to ornithine (K(m) = 1.2 millimolar) as well as to carbamoyl phosphate (K(m) = 0.2 millimolar). The approximate molecular weight determined by gel sieving was 77,600.A desalted ammonium sulfate precipitate from 14-day seedlings (inclusive roots and senescing cotyledons) was applied on a column of anion exchanger. The elution pattern showed one peak of ornithine carbamoyl-transferase activity. This elution pattern was the same as observed for the enzyme from chloroplasts.The results suggest the presence of one form of ornithine carbamoyl-transferase in pea seedlings.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

de Ruiter H,Kollöffel C

doi

10.1104/pp.77.3.695

subject

Has Abstract

pub_date

1985-03-01 00:00:00

pages

695-9

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

77

pub_type

杂志文章
  • Soluble and Membrane-Bound β-Glucosidases Are Involved in Trimming the Xyloglucan Backbone.

    abstract::In many flowering plants, xyloglucan is a major component of primary cell walls, where it plays an important role in growth regulation. Xyloglucan can be degraded by a suite of exoglycosidases that remove specific sugars. In this work, we show that the xyloglucan backbone, formed by (1→4)-linked β-d-glucopyranosyl res...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01713

    authors: Sampedro J,Valdivia ER,Fraga P,Iglesias N,Revilla G,Zarra I

    更新日期:2017-02-01 00:00:00

  • Peridinin-Chlorophyll a Proteins of the Dinoflagellate Amphidinium carterae (Plymouth 450).

    abstract::The marine dinoflagellate Amphidinium carterae (Plymouth 450) releases several water-soluble peridinin-chlorophyll a proteins after freezethawing. These chromoproteins have a molecular weight of 39.2 x 10(3) and are comprised of noncovalently bound peridinin and chlorophyll a and a nonoligomeric protein. They have dis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.2.297

    authors: Haxo FT,Kycia JH,Somers GF,Bennett A,Siegelman HW

    更新日期:1976-02-01 00:00:00

  • Development of a radioimmunoassay for the soybean phytoalexin glyceollin I.

    abstract::A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.2.233

    authors: Moesta P,Hahn MG,Grisebach H

    更新日期:1983-10-01 00:00:00

  • Galactinol synthase1. A novel heat shock factor target gene responsible for heat-induced synthesis of raffinose family oligosaccharides in Arabidopsis.

    abstract::Heat shock factors (HSFs) are transcriptional regulators of the heat shock response. The major target of HSFs are the genes encoding heat shock proteins (HSPs), which are known to have a protective function that counteracts cytotoxic effects. To identify other HSF target genes, which may be important determinants for ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.042606

    authors: Panikulangara TJ,Eggers-Schumacher G,Wunderlich M,Stransky H,Schöffl F

    更新日期:2004-10-01 00:00:00

  • Malate Metabolism in Leaf Mitochondria from the Crassulacean Acid Metabolism Plant Kalanchoë blossfeldiana Poelln.

    abstract::The mechanisms and the controlling factors of malate oxidation by mitochondria from leaves of Kalanchoë blossfeldiana Poelln. plants performing Crassulacean acid metabolism were investigated using Percollpurified mitochondria. The effects of pH and of various cofactors (ATP, NAD(+), coenzyme A) on malate dehydrogenase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.4.1039

    authors: Rustin P,Lance C

    更新日期:1986-08-01 00:00:00

  • Vacuolar contents of fruit subepidermal cells from vitis species.

    abstract::Enzymic treatment of mature, anthocyanin-containing grape berry sub-epidermal tissues from DeChaunac grapes released intact protoplasts. Filtration of the protoplast suspension through glass wool under mild suction resulted in the release of vacuoles. The total and individual contents of anthocyanins, flavonol glycosi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.3.686

    authors: Moskowitz AH,Hrazdina G

    更新日期:1981-09-01 00:00:00

  • Isolation and characterization of glutathione S-transferase isozymes from sorghum.

    abstract::Two glutathione S-transferase (GST) isozymes, A1/A1 and B1/B2, were purified from etiolated, O-1,3-dioxolan-2-yl-methyl-2,2,2, -trifluoro-4'-chloroacetophenone-oxime-treated sorghum (Sorghum bicolor L. Moench) shoots. GST A1/A1, a constitutively expressed homodimer, had a subunit molecular mass of 26 kD and an isoelec...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.3.877

    authors: Gronwald JW,Plaisance KL

    更新日期:1998-07-01 00:00:00

  • Effects of CO(2) Concentration on Rubisco Activity, Amount, and Photosynthesis in Soybean Leaves.

    abstract::Growth at an elevated CO(2) concentration resulted in an enhanced capacity for soybean (Glycine max L. Merr. cv Bragg) leaflet photosynthesis. Plants were grown from seed in outdoor controlled-environment chambers under natural solar irradiance. Photosynthetic rates, measured during the seed filling stage, were up to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.4.1310

    authors: Campbell WJ,Allen LH,Bowes G

    更新日期:1988-12-01 00:00:00

  • Kok effect and the quantum yield of photosynthesis : light partially inhibits dark respiration.

    abstract::The linear response of photosynthesis to light at low photon flux densities is known to change abruptly in the vicinity of the light compensation point so that the quantum yield seems to decrease as radiation increases. We studied this ;Kok effect' in attached sunflower (Helianthus annuus L. cv IS894) leaves using gas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.1.95

    authors: Sharp RE,Matthews MA,Boyer JS

    更新日期:1984-05-01 00:00:00

  • Does the Induction of Flowering by Photoperiod Change the Polarity or Other Characteristics of Indole-3-acetic Acid Transport in Petioles for the Short Day Plant, Xanthium?

    abstract::To test the hypothesis that photoinduction acts by changing the ability of the plant to transport hormones, rather than by changing the ability of organs to synthesize them, the transport of carboxy-labeled indole-3-acetic acid was measured in the short day plant Xanthium pensylvanicum. Plants grown under noninductive...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.3.307

    authors: Jacobs WP

    更新日期:1978-03-01 00:00:00

  • FLAVODIIRON2 and FLAVODIIRON4 proteins mediate an oxygen-dependent alternative electron flow in Synechocystis sp. PCC 6803 under CO2-limited conditions.

    abstract::This study aims to elucidate the molecular mechanism of an alternative electron flow (AEF) functioning under suppressed (CO2-limited) photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], reaches a maximum shortly...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.249987

    authors: Shimakawa G,Shaku K,Nishi A,Hayashi R,Yamamoto H,Sakamoto K,Makino A,Miyake C

    更新日期:2015-02-01 00:00:00

  • Effect of Night Temperature on the Activity of Sucrose Phosphate Synthase, Acid Invertase, and Sucrose Synthase in Source and Sink Tissues of Rosa hybrida cv Golden Times.

    abstract::Sucrose phosphate synthase and acid invertase activities in the mature leaves of roses (Rosa hybrida cv Golden Times) were greater in plants grown under a higher night temperature than under a lower temperature regime. In young shoots, the activity of acid invertase was promoted by the lower temperature while that of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.2.447

    authors: Khayat E,Zieslin N

    更新日期:1987-06-01 00:00:00

  • Characterization of a molecular modification of phytochrome that is associated with its conversion to the far-red-absorbing form.

    abstract::Phytochrome that has been photoinduced to pellet by irradiation of intact oat (cv. Garry) shoots and recovered from a pellet obtained by centrifugation of crude extracts exhibits modified behavior when compared to soluble phytochrome isolated from shoots that had never been irradiated. This modified behavior includes ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.4.789

    authors: Boeshore ML,Pratt LH

    更新日期:1981-10-01 00:00:00

  • Double knockout mutants of Arabidopsis grown under normal conditions reveal that the plastidial phosphorylase isozyme participates in transitory starch metabolism.

    abstract::In leaves of two starch-related single-knockout lines lacking either the cytosolic transglucosidase (also designated as disproportionating enzyme 2, DPE2) or the maltose transporter (MEX1), the activity of the plastidial phosphorylase isozyme (PHS1) is increased. In both mutants, metabolism of starch-derived maltose i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.227843

    authors: Malinova I,Mahlow S,Alseekh S,Orawetz T,Fernie AR,Baumann O,Steup M,Fettke J

    更新日期:2014-02-01 00:00:00

  • Ion fluxes and abscisic Acid-induced proline accumulation in barley leaf segments.

    abstract::The increase in proline induced by ABA, a process stimulated by NaCl or KCl in barley leaves, did not occur when Na(+) (or K(+)) was present in the external medium as the gluconate salt, namely with an anion unable to permeate the plasma membrane. However, proline increase was restored, to different extents, by the ad...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.3.927

    authors: Pesci P

    更新日期:1988-03-01 00:00:00

  • The isolation of spinach chloroplasts in pyrophosphate media.

    abstract::A simplified procedure (involving disruption in sorbitol-pyrophosphate mixtures) permits the separation of spinach chloroplasts which retain the ability to catalyze the photosynthetic assimilation of carbon dioxide and its associated oxygen evolution. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.9.1415

    authors: Cockburn W,Walker DA,Baldry CW

    更新日期:1968-09-01 00:00:00

  • Effects of chilling on the biochemical and functional properties of thylakoid membranes.

    abstract::The mechanism of chilling resistance was investigated in 4-week-old plants of the chilling-sensitive cultivated tomato, Lycopersicon esculentum Mill. cv H722, and rooted cuttings of its chilling-resistant wild relative, L. hirsutum Humb. and Bonpl., which were chilled for 3 days at 2 degrees C with a 14-hour photoperi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.2.663

    authors: Walker MA,McKersie BD,Pauls KP

    更新日期:1991-10-01 00:00:00

  • l-Phenylalanine Ammonia-lyase (Maize): Partial Purification and Response to Gibberellic Acid and Cycloheximide of l-Phenylalanine and l-Tyrosine Ammonia-lyase Activities.

    abstract::Extracts of maize leaf sheath tissue deaminate both l-phenylalanine and l-tyrosine. The activities with both substrates are enhanced by treating the plant with gibberellic acid. Both activities decrease rapidly at the same rate when tissue is incubated in a moist atmosphere, and this decrease can be slowed by treatmen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.4.480

    authors: Reid PD

    更新日期:1972-10-01 00:00:00

  • WEREWOLF, a regulator of root hair pattern formation, controls flowering time through the regulation of FT mRNA stability.

    abstract::A key floral activator, FT, integrates stimuli from long-day, vernalization, and autonomous pathways and triggers flowering by directly regulating floral meristem identity genes in Arabidopsis (Arabidopsis thaliana). Since a small amount of FT transcript is sufficient for flowering, the FT level is strictly regulated ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.176685

    authors: Seo E,Yu J,Ryu KH,Lee MM,Lee I

    更新日期:2011-08-01 00:00:00

  • Trehalose Toxicity in Cuscuta reflexa: CORRELATION WITH LOW TREHALASE ACTIVITY.

    abstract::A toxic effect of alpha,alpha-trehalose in an angiospermic plant, Cuscuta reflexa (dodder), is described. This disaccharide and its analogs, 2-aminotrehalose and 4-aminotrehalose, induced a rapid blackening of the terminal region of the vine which is involved in elongation growth. From the results of in vitro growth o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1369

    authors: Veluthambi K,Mahadevan S,Maheshwari R

    更新日期:1981-12-01 00:00:00

  • Phosphatidylinositol 4,5-Bisphosphate Phospholipase C and Phosphomonoesterase in Dunaliella salina Membranes.

    abstract::In comparison with other cell organelles, the Dunaliella salina plasma membrane was found to be highly enriched in phospholipase C activity toward exogenous [(3)H]phosphatidylinositol 4,5-bisphosphate (PIP(2)). Based on release of [(3)H]inositol phosphates, the plasma membrane exhibited a PIP(2)-phospholipase C activi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.1115

    authors: Einspahr KJ,Peeler TC,Thompson GA

    更新日期:1989-07-01 00:00:00

  • Identification of plant hormones from cotton ovules.

    abstract::An extract from 8-day-old cotton ovules (Gossypium hirsutum L.) was partitioned into three fractions and each fraction was derivatized and analyzed separately. Gas-liquid chromatography and computer-controlled gas-liquid chromatography-mass spectrometry were used to separate, measure, and identify the naturally occurr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.3.550

    authors: Shindy WW,Smith OE

    更新日期:1975-03-01 00:00:00

  • Sulfur economy and cell wall biosynthesis during sulfur limitation of Chlamydomonas reinhardtii.

    abstract::We have identified two novel periplasmic/cell wall polypeptides that specifically accumulate during sulfur limitation of Chlamydomonas reinhardtii. These polypeptides, present at high levels in the extracellular polypeptide fraction from a sulfur-deprived, cell wall-minus C. reinhardtii strain, have apparent molecular...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Takahashi H,Braby CE,Grossman AR

    更新日期:2001-10-01 00:00:00

  • Unique Motifs and Length of Hairpin in Oleosin Target the Cytosolic Side of Endoplasmic Reticulum and Budding Lipid Droplet.

    abstract::Plant cytosolic lipid droplets (LDs) are covered with a layer of phospholipids and oleosin and were extensively studied before those in mammals and yeast. Oleosin has short amphipathic N- and C-terminal peptides flanking a conserved 72-residue hydrophobic hairpin, which penetrates and stabilizes the LD Oleosin is synt...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00366

    authors: Huang CY,Huang AHC

    更新日期:2017-08-01 00:00:00

  • Endogenous abscisic acid as a key switch for natural variation in flooding-induced shoot elongation.

    abstract::Elongation of leaves and stem is a key trait for survival of terrestrial plants during shallow but prolonged floods that completely submerge the shoot. However, natural floods at different locations vary strongly in duration and depth, and, therefore, populations from these locations are subjected to different selecti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.162792

    authors: Chen X,Pierik R,Peeters AJ,Poorter H,Visser EJ,Huber H,de Kroon H,Voesenek LA

    更新日期:2010-10-01 00:00:00

  • Identification of Putative Substrates of SEC2, a Chloroplast Inner Envelope Translocase.

    abstract::Most chloroplast proteins are synthesized in the cytosol and imported into chloroplasts. Many imported proteins are further targeted to the thylakoid membrane and lumen by the SEC1, TAT, or SRP/ALB3 translocases. Others are targeted to the inner chloroplast envelope membrane by undescribed translocases. Recently, a se...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00012

    authors: Li Y,Martin JR,Aldama GA,Fernandez DE,Cline K

    更新日期:2017-04-01 00:00:00

  • The histidine kinase Hik34 is involved in thermotolerance by regulating the expression of heat shock genes in synechocystis.

    abstract::Histidine kinases (Hiks) in Synechocystis sp. PCC 6803 are involved in the transduction of signals associated with various kinds of environmental stress. To examine the potential role in thermotolerance of Hiks, we used genome microarray analysis to screen a Hik knockout library for mutations that affected the express...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.059097

    authors: Suzuki I,Kanesaki Y,Hayashi H,Hall JJ,Simon WJ,Slabas AR,Murata N

    更新日期:2005-07-01 00:00:00

  • Early Gravi-Electrical Responses in Bean Epicotyls.

    abstract::The relationship between gravitropism and surface electrical potentials was studied using etiolated epicotyls of adzuki bean (Phaseolus angularis). Early downward curvature (or transient positive gravitropic response) was observed about 1 min after gravistimulation. The downward curvature was closely related to the sp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.3.875

    authors: Shigematsu H,Toko K,Matsuno T,Yamafuji K

    更新日期:1994-07-01 00:00:00

  • Development of Enzymes Involved in Photosynthetic Carbon Assimilation in Greening Seedlings of Maize (Zea mays).

    abstract::Upon illumination of dark-grown maize seedlings (5 days old) with incandescent light, there occurred a nearly simultaneous increase, after a certain lag period, in the activities of enzymes engaged in the C(4) pathway and the Calvin-Benson cycle. The light-induced biosynthesis of chlorophyll (a and b) precedes the inc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.2.198

    authors: Kobayashi H,Asami S,Akazawa T

    更新日期:1980-02-01 00:00:00

  • Light-induced growth promotion by SPA1 counteracts phytochrome-mediated growth inhibition during de-etiolation.

    abstract::Previous evidence has suggested that SPA1 is a signal transduction component that appears to require phytochrome A for function in seedling photomorphogenesis. Using digital image analysis, we examined the time course of growth inhibition induced by red light in spa1 mutants to test the interpretation that SPA1 functi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.3.1291

    authors: Parks BM,Hoecker U,Spalding EP

    更新日期:2001-07-01 00:00:00