Development of a radioimmunoassay for the soybean phytoalexin glyceollin I.

abstract：:Fruit removal for 5 weeks after flowering increased acid phosphatase activity 10-fold in soybean (Glycine max L. Merr. Var Hobbit) leaves compared with normal seed-pod-bearing plants. The major acid phosphatase activity in leaves was purified over 2700-fold, yielding a single polypeptide of 51 kD with a specific activ...

journal_title：Plant physiology

authors： Staswick PE,Papa C,Huang JF,Rhee Y

更新日期：1994-01-01 00:00:00

abstract：:A comparative study was made of the effects of auxin (alpha-naphthalene acetic acid), kinetin (6-furfurylaminopurine) and a mixture of auxin and kinetin applied in vivo on synthesis of RNA and protein and the distribution of such synthesis amongst the subcellular fractions of sections of endocarp from Kentucky Wonder ...

journal_title：Plant physiology

authors： Sacher JA

更新日期：1967-10-01 00:00:00

abstract：:Root nodules were harvested from chamber-grown soybean (Glycine max L. Merrill cv Woodworth) plants throughout development. Apparent nitrogenase activity (acetylene reduction) peaked before seeds began to develop, but a significant amount of activity remained as the seeds matured. Nodule senescence was defined as the ...

journal_title：Plant physiology

authors： Pfeiffer NE,Torres CM,Wagner FW

更新日期：1983-04-01 00:00:00

abstract：:The oat (Avena sativa L.) seed globulin was found to be synthesized in vitro as 60,000 to 64,000 dalton precursors. In vivo protein labeling yielded polypeptides of 58,000 to 62,000 daltons, suggesting cleavage of signal sequences from the precursors. Further cleavage is apparently required to separate the alpha and b...

journal_title：Plant physiology

authors： Brinegar AC,Peterson DM

更新日期：1982-12-01 00:00:00

abstract：:The phytohormone auxin regulates virtually every aspect of plant development. The hormone directly mediates the interaction between the two members of the auxin coreceptor complex, a TRANSPORT INHIBITOR RESPONSE (TIR1)/AUXIN SIGNALING F-BOX protein and an AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressor....

journal_title：Plant physiology

authors： Yu H,Moss BL,Jang SS,Prigge M,Klavins E,Nemhauser JL,Estelle M

更新日期：2013-05-01 00:00:00

abstract：:The addition of primary amines to the growth medium of the unicellular green alga Chlamydomonas reinhardtii disrupts cell wall assembly in both vegetative and zygotic cells. Primary amines are competitive inhibitors of the protein-cross-linking activity of transglutaminases. Two independent assays for transglutaminase...

journal_title：Plant physiology

authors： Waffenschmidt S,Kusch T,Woessner JP

更新日期：1999-11-01 00:00:00

abstract：:Sucrose:sucrose 1-fructosyltransferase (1-SST), an enzyme involved in fructan biosynthesis, was purified to homogeneity from tubers of Helianthus tuberosus that were harvested in the accumulation phase. Gel filtration under native conditions predicted a molecular mass of about 67 kD. Electrophoresis or gel filtration ...

journal_title：Plant physiology

authors： Koops AJ,Jonker HH

更新日期：1996-04-01 00:00:00

abstract：:The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activi...

journal_title：Plant physiology

authors： Sumida S,Mudd JB

更新日期：1970-06-01 00:00:00

abstract：:Allene oxide synthase (AOS; hydroperoxide dehydratase; EC 4.2.1.92) catalyzes the first step in the biosynthesis of jasmonic acid from lipoxygenase-derived hydroperoxides of free fatty acids. Using the AOS cDNA from tomato (Lycopersicon esculentum), in which the role of jasmonic acid in wound-induced defense gene acti...

journal_title：Plant physiology

authors： Sivasankar S,Sheldrick B,Rothstein SJ

更新日期：2000-04-01 00:00:00

abstract：:The plant-specific translation initiation complex eIFiso4F is encoded by three genes in Arabidopsis (Arabidopsis thaliana)-genes encoding the cap binding protein eIFiso4E (eifiso4e) and two isoforms of the large subunit scaffolding protein eIFiso4G (i4g1 and i4g2). To quantitate phenotypic changes, a phenomics platfor...

journal_title：Plant physiology

authors： Lellis AD,Patrick RM,Mayberry LK,Lorence A,Campbell ZC,Roose JL,Frankel LK,Bricker TM,Hellmann HA,Mayberry RW,Zavala AS,Choy GS,Wylie DC,Abdul-Moheeth M,Masood A,Prater AG,Van Hoorn HE,Cole NA,Browning KS

更新日期：2019-09-01 00:00:00

abstract：:Ethanol addition or the exposure of dark-grown resting Euglena to light produced a transient increase in the rate of fumarase synthesis as measured by pulse-labeling with [(35)S]sulfate and immunoprecipitation. The half-life of fumarase in the dark and in the light was similar. In the plastidless mutant W(3)BUL, fumar...

journal_title：Plant physiology

authors： Rikin A,Schwartzbach SD

更新日期：1989-05-01 00:00:00

abstract：:Nonautotrophic CO(2) metabolism in Opuntia echinocarpa roots was studied with techniques of manometry and radiometry. The roots were grown in a one-quarter strength nutrient solution for several days; the distal 2 cm was used for physiological studies. The roots assimilated significant quantities of (14)CO(2) and appe...

journal_title：Plant physiology

authors： Ting IP,Dugger WM

更新日期：1966-03-01 00:00:00

abstract：:Following excision, etiolated epicotyl segments of Pisum sativum L. cv. Alaska exhibit a marked hyperpolarization of membrane potential which is followed by a linear accumulation of K(+) when segments are incubated in Higinbotham nutrient solution. Segments aged for several hours and then reexcised display only a slig...

journal_title：Plant physiology

authors： Pierce WS

更新日期：1981-04-01 00:00:00

abstract：:Tryptophan decarboxylase (TDC) is a cytosolic enzyme that catalyzes an early step of the terpenoid indole alkaloid biosynthetic pathway by decarboxylation of L-tryptophan to produce the protoalkaloid tryptamine. In the present study, recombinant TDC was targeted to the chloroplast, cytosol, and endoplasmic reticulum (...

journal_title：Plant physiology

authors： Di Fiore S,Li Q,Leech MJ,Schuster F,Emans N,Fischer R,Schillberg S

更新日期：2002-07-01 00:00:00

abstract：:Formation of the chlorophyll and heme precursor delta-aminolevulinic acid (ALA) from glutamate in soluble extracts of Chlorella vulgaris, Euglena gracilis, and Cyanidium caldarium was stimulated by addition of low molecular weight RNA derived from greening algae or plant tissue. Enzyme extracts were prepared for the A...

journal_title：Plant physiology

authors： Weinstein JD,Mayer SM,Beale SI

更新日期：1986-12-01 00:00:00

abstract：:Synechocystis (PCC 6714) is photoinhibited at low photon flux density when illuminated in medium devoid of Na(+). Photosynthetic activity and variable fluorescence yield were measured in intact cells during the course of inhibition and during recovery from inhibition in order to characterize sequential inhibitory even...

journal_title：Plant physiology

authors： Zhao J,Brand JJ

更新日期：1989-09-01 00:00:00

abstract：:Extracts of resting and germinating (3 days at 20 degrees C) wheat (Triticum aestivum L. cv Ruso) grains rapidly hydrolyzed various benzyloxycarbonyldipeptides (Z-dipeptides) at pH 4 to 6. Similar activities were present in extracts of mature flag leaves. Fractionation by chromatography on CM-cellulose and on Sephadex...

journal_title：Plant physiology

authors： Mikola L

更新日期：1986-07-01 00:00:00

abstract：:A chimeric gene containing the patatin promoter and the transit-peptide region of the small-subunit carboxylase gene was utilized to direct expression of Escherichia coli glycogen synthase (glgA) to potato (Solanum tuberosum) tuber amyloplasts. Expression of the glgA gene product in tuber amyloplasts was between 0.007...

journal_title：Plant physiology

authors： Shewmaker CK,Boyer CD,Wiesenborn DP,Thompson DB,Boersig MR,Oakes JV,Stalker DM

更新日期：1994-04-01 00:00:00

abstract：:In maize (Zea mays) and other grasses, changes in orientation of stems are perceived by pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. The amyloplast-containing bundle sheath cells are the sites of gravity perception, although the initial steps of gravit...

journal_title：Plant physiology

authors： Johannes E,Collings DA,Rink JC,Allen NS

更新日期：2001-09-01 00:00:00

abstract：:The mesophyll of sugar beet (Beta vulgaris L.) leaves emits red (chlorophyll a) fluorescence and blue-green fluorescence when excited with ultraviolet light. The intensity of blue-green fluorescence was increased in mesophylls affected by iron deficiency. This increase was large and progressive. It was concomitant wit...

journal_title：Plant physiology

authors： Morales F,Cerovic ZG,Moya I

更新日期：1994-09-01 00:00:00

abstract：:In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in imma...

journal_title：Plant physiology

authors： Ciavatta VT,Morillon R,Pullman GS,Chrispeels MJ,Cairney J

更新日期：2001-12-01 00:00:00

abstract：:Biosynthesis of sucrose from triacylglycerol requires the bypass of the CO2-evolving reactions of the tricarboxylic acid (TCA) cycle. The regulation of the TCA cycle bypass during lipid mobilization was examined. Lipid mobilization in Brassica napus was initiated shortly after imbibition of the seed and proceeded unti...

journal_title：Plant physiology

authors： Falk KL,Behal RH,Xiang C,Oliver DJ

更新日期：1998-06-01 00:00:00

abstract：:A phosphatidylinositol-specific phospholipase C of plant stems (EC 3.1.4.10) assayed at pH 6.6 and at 30 degrees C cleaved phosphatidylinositol such that more than 85% of the product was inositol-1-phosphate. Other phospholipids were cleaved 5 to 10% or less under these conditions. The phospholipase had both a soluble...

journal_title：Plant physiology

authors： Pfaffmann H,Hartmann E,Brightman AO,Morré DJ

更新日期：1987-12-01 00:00:00

abstract：:Leaves of woad (Isatis tinctoria L.) were found to incorporate efficiently tritiated indoleacetaldoxime and (35)S from (35)S-l-cystine into glucobrassicin and sulfoglucobrassicin. Time course of incorporation of (35)S from (35)S-cystine into the glucosinolates indicated that glucobrassicin was formed first and then su...

journal_title：Plant physiology

authors： Mahadevan S,Stowe BB

更新日期：1972-07-01 00:00:00

abstract：:The pattern of endoproteolytic activities occurring during wheat (Triticum aestivum, cultivar Chinese Spring) grain development was investigated. Total endoprotease activity, assayed in solution with azocasein as a substrate, increased during the early stages of grain development to reach a maximum at 15 d postanthesi...

journal_title：Plant physiology

authors： Dominguez F,Cejudo FJ

更新日期：1996-11-01 00:00:00

abstract：:A full-genome microarray of the (oxy)photosynthetic cyanobacterium Synechocystis sp. PCC 6803 was used to identify genes that were transcriptionally regulated by growth in iron (Fe)-deficient versus Fe-sufficient media. Transcript accumulation for 3,165 genes in the genome was analyzed using an analysis of variance mo...

journal_title：Plant physiology

authors： Singh AK,McIntyre LM,Sherman LA

更新日期：2003-08-01 00:00:00

abstract：:Lys deacylases are essential regulators of cell biology in many contexts. Here, we have identified CddA (cyanobacterial deacetylase/depropionylase), a Lys deacylase enzyme expressed in the cyanobacterium Synechococcus sp. PCC 7002 that has both deacetylase and depropionylase activity. Loss of the gene cddA led to slow...

journal_title：Plant physiology

authors： Liu X,Yang M,Liu Y,Ge F,Zhao J

更新日期：2020-10-01 00:00:00

abstract：:Root and stem segments from soybean (Merrill cv. ;Bragg') showed an enhanced chemiluminescence upon mechanical injury. Roots emitted more light than did stems. Light emission was diminished by CN(-) and N(3) (-) but was not affected by rotenone and antimycin A. Catalase quenched chemiluminescence in wounded root segme...

journal_title：Plant physiology

authors： Salin ML,Bridges SM

更新日期：1981-01-01 00:00:00

abstract：:The response of cortical microtubules to low temperature and freezing was assessed for root tips of cold-acclimated and non-acclimated winter rye (Secale cereale L. cv Puma) seedlings using indirect immunofluorescence microscopy with antitubulin antibodies. Roots cooled to 0 or -3 degrees C were fixed for immunofluore...

journal_title：Plant physiology

authors： Kerr GP,Carter JV

更新日期：1990-05-01 00:00:00

abstract：:When tobacco (Nicotiana tabacum L. var. Virginia Gold) plants were pretreated with Na ((22)Na) several days before detopping, from 2.3 to 4.9% of Na previously accumulated in roots appeared in the xylem exudate in 7 days after detopping. Na from the external medium, however, was readily transported to the exudate. Mor...

journal_title：Plant physiology

authors： Wallace A,Abou-Zamzam AM,Mueller RT

更新日期：1972-09-01 00:00:00