Metabolic bypass of the tricarboxylic acid cycle during lipid mobilization in germinating oilseeds. Regulation Of nad+-dependent isocitrate dehydrogenase versus fumarase


:Biosynthesis of sucrose from triacylglycerol requires the bypass of the CO2-evolving reactions of the tricarboxylic acid (TCA) cycle. The regulation of the TCA cycle bypass during lipid mobilization was examined. Lipid mobilization in Brassica napus was initiated shortly after imbibition of the seed and proceeded until 2 d postimbibition, as measured by in vivo [1-14C]acetate feeding to whole seedlings. The activity of NAD+-isocitrate dehydrogenase (a decarboxylative enzyme) was not detected until 2 d postimbibition. RNA-blot analysis of B. napus seedlings demonstrated that the mRNA for NAD+-isocitrate dehydrogenase was present in dry seeds and that its level increased through the 4 d of the experiment. This suggested that NAD+-isocitrate dehydrogenase activity was regulated by posttranscriptional mechanisms during early seedling development but was controlled by mRNA level after the 2nd or 3rd d. The activity of fumarase (a component of the nonbypassed section of the TCA cycle) was low but detectable in B. napus seedlings at 12 h postimbibition, coincident with germination, and increased for the next 4 d. RNA-blot analysis suggested that fumarase activity was regulated primarily by the level of its mRNA during germination and early seedling development. It is concluded that posttranscriptional regulation of NAD+-isocitrate dehydrogenase activity is one mechanism of restricting carbon flux through the decarboxylative section of the TCA cycle during lipid mobilization in germinating oilseeds.


Plant Physiol


Plant physiology


Falk KL,Behal RH,Xiang C,Oliver DJ




Has Abstract


1998-06-01 00:00:00












  • Inhibition by fusicoccin of germination of pea seeds.

    abstract::Fusicoccin inhibits the germination of pea (Pisum sativum L. cv Progress 9) seeds by decreasing the growth of the embryonal axis and by stimulating the fresh weight increase of the cotyledons. The growth of isolated embryonal axes in the presence of sucrose and KCl is stimulated by fusicoccin. The effect of fusicoccin...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lioi L,Petruzzelli L,Morgutti S,Cocucci SM

    更新日期:1984-11-01 00:00:00

  • Effect of arabinogalactan proteins from the root caps of pea and Brassica napus on Aphanomyces euteiches zoospore chemotaxis and germination.

    abstract::Root tips of many plant species release a number of border, or border-like, cells that are thought to play a major role in the protection of root meristem. However, little is currently known on the structure and function of the cell wall components of such root cells. Here, we investigate the sugar composition of the ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cannesan MA,Durand C,Burel C,Gangneux C,Lerouge P,Ishii T,Laval K,Follet-Gueye ML,Driouich A,Vicré-Gibouin M

    更新日期:2012-08-01 00:00:00

  • Calcium Requirement for Indoleacetic Acid-induced Acidification by Avena Coleoptiles.

    abstract::The ionic specificity of IAA-induced acidification by Avena coleoptiles was studied, using zwitterionic, presumably impermeant buffers. The acidification was almost totally dependent on divalent cations with an order of effectiveness of Ca(2+) >/= Sr(2+) > Mn(2+), Mg(2+); whereas other polyvalent cations tested were i...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cohen JD,Nadler KD

    更新日期:1976-03-01 00:00:00

  • Characteristics of teratomas regenerated in vitro from octopine-type crown gall.

    abstract::Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growi...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Owens LD

    更新日期:1982-01-01 00:00:00

  • Saturation Kinetics of the Velocity of Stomatal Closing in Response to CO(2).

    abstract::Stomatal closing movements in response to changes from CO(2)-free to CO(2)-containing air were recorded in leaf sections of Zea mays using air flow porometers. The response to CO(2) was fast; the shortest lag between the application of 300 microliters CO(2) per liter of air and the beginning of a stomatal response was...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Raschke K

    更新日期:1972-02-01 00:00:00

  • Functional analysis of the final steps of the 1-deoxy-D-xylulose 5-phosphate (DXP) pathway to isoprenoids in plants using virus-induced gene silencing.

    abstract::Isoprenoid biosynthesis in plant plastids occurs via the 1-deoxy-d-xylulose 5-phosphate (DXP) pathway. We used tobacco rattle virus (TRV) to posttranscriptionally silence the expression of the last two enzymes of this pathway, the IspG-encoded (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS) and the IspH-en...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Page JE,Hause G,Raschke M,Gao W,Schmidt J,Zenk MH,Kutchan TM

    更新日期:2004-04-01 00:00:00

  • Biosynthetic Origin of Gibberellins A(3) and A(7) in Cell-Free Preparations from Seeds of Marah macrocarpus and Malus domestica.

    abstract::Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A(9) (GA(9)) and 2,3-dehydroGA(9) to GA(7); GA(9) was also metabolized to GA(4) in a branch pathway. The preparation from Marah seeds also metabolized GA(5) to GA(3) in high yield; GA(6) was a minor...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Albone KS,Gaskin P,Macmillan J,Phinney BO,Willis CL

    更新日期:1990-09-01 00:00:00

  • Metabolic engineering of the chloroplast genome using the Echerichia coli ubiC gene reveals that chorismate is a readily abundant plant precursor for p-hydroxybenzoic acid biosynthesis.

    abstract::p-Hydroxybenzoic acid (pHBA) is the major monomer in liquid crystal polymers. In this study, the Escherichia coli ubiC gene that codes for chorismate pyruvate-lyase (CPL) was integrated into the tobacco (Nicotiana tabacum) chloroplast genome under the control of the light-regulated psbA 5' untranslated region. CPL cat...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Viitanen PV,Devine AL,Khan MS,Deuel DL,Van Dyk DE,Daniell H

    更新日期:2004-12-01 00:00:00

  • Ethylene production by auxin-deprived, suspension-cultured pear fruit cells in response to auxins, stress, or precursor.

    abstract::Auxin-deprived, mannitol-supplemented, suspension-cultured pear (Pyrus communis L. Passe Crassane) fruit cells produce large quantities (20-40 nanoliters ethylene per 10(6) cells per hour) of ethylene in response to auxins, CuCl(2) or 1-amino-cyclopropane-1-carboxylic acid (ACC). Maximum rates of production are achiev...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Puschmann R,Romani R

    更新日期:1983-12-01 00:00:00

  • Alpha-amylase synthesis in wheat kernels as influenced by the seed coat.

    abstract::The effect of seed coat removal on the synthesis of alpha-amylase isoenzymes in wheat was investigated. The immature wheat endosperm-aleurone (seed coat and embryo detached) produced considerably less alpha-amylase activity than immature whole or de-embryonated wheat kernels, when incubated under identical conditions ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Marchylo BA,Lacroix LJ,Kruger JE

    更新日期:1981-01-01 00:00:00

  • ADP/ATP Translocator from Pea Root Plastids (Comparison with Translocators from Spinach Chloroplasts and Pea Leaf Mitochondria).

    abstract::The kinetic properties of the adenosine 5[prime]-diphosphate/adenosine 5[prime]-triphosphate (ADP/ATP) translocator from pea (Pisum sativum L.) root plastids were determined by silicone oil filtering centrifugation and compared with those of spinach (Spinacia oleracea L.) chloroplasts and pea leaf mitochondria. In add...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Schunemann D,Borchert S,Flugge UI,Heldt HW

    更新日期:1993-09-01 00:00:00

  • Kinetic and spectral resolution of multiple nonphotochemical quenching components in Arabidopsis leaves.

    abstract::Using novel specially designed instrumentation, fluorescence emission spectra were recorded from Arabidopsis (Arabidopsis thaliana) leaves during the induction period of dark to high-light adaptation in order to follow the spectral changes associated with the formation of nonphotochemical quenching. In addition to an ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lambrev PH,Nilkens M,Miloslavina Y,Jahns P,Holzwarth AR

    更新日期:2010-03-01 00:00:00

  • Myosin XI-K Is required for rapid trafficking of Golgi stacks, peroxisomes, and mitochondria in leaf cells of Nicotiana benthamiana.

    abstract::A prominent feature of plant cells is the rapid, incessant movement of the organelles traditionally defined as cytoplasmic streaming and attributed to actomyosin motility. We sequenced six complete Nicotiana benthamiana cDNAs that encode class XI and class VIII myosins. Phylogenetic analysis indicates that these two c...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Avisar D,Prokhnevsky AI,Makarova KS,Koonin EV,Dolja VV

    更新日期:2008-03-01 00:00:00

  • Phytoalexin Elicitor Activity of Carbohydrates from Phytophthora megasperma f.sp. glycinea and Other Sources.

    abstract::Three unique classes of carbohydrates were isolated from the hyphal cell walls of Phytophthora megasperma f.sp. glycinea (Pmg) and compared with other substances for their activity as elicitors of the phytoalexin glyceollin in soybean tissues. Glucomannans extracted from cell walls with soybean beta-1,3-endoglucanase ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Keen NT,Yoshikawa M,Wang MC

    更新日期:1983-03-01 00:00:00

  • Cytochrome c is released in a reactive oxygen species-dependent manner and is degraded via caspase-like proteases in tobacco Bright-Yellow 2 cells en route to heat shock-induced cell death.

    abstract::To gain some insight into the mechanism of plant programmed cell death, certain features of cytochrome c (cyt c) release were investigated in heat-shocked tobacco (Nicotiana tabacum) Bright-Yellow 2 cells in the 2- to 6-h time range. We found that 2 h after heat shock, cyt c is released from intact mitochondria into t...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Vacca RA,Valenti D,Bobba A,Merafina RS,Passarella S,Marra E

    更新日期:2006-05-01 00:00:00

  • Cytokinin-to-Auxin Ratios and Morphology of Shoots and Tissues Transformed by a Chimeric Isopentenyl Transferase Gene.

    abstract::Tissues transformed with the isopentenyl transferase (ipt) gene cloned from the T-DNA region of the Ti plasmid or with the ipt gene placed under the control of the cauliflower mosaic virus 35S promoter (35S-ipt) were analyzed for auxin and cytokinin. Free and total indole-3-acetic acid (IAA) levels in 35S-ipt-transfor...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Smigocki AC,Owens LD

    更新日期:1989-11-01 00:00:00

  • Regulation of Gene Expression by Ethylene in Wild-Type and rin Tomato (Lycopersicon esculentum) Fruit.

    abstract::Levels of ethylene biosynthesis and ethylene-inducible gene expression in wild-type tomato (Lycopersicon esculentum) fruit and in nonripening fruit from the tomato mutant rin (ripening inhibitor) were compared in order to investigate the mechanism of ethylene action. Whereas wild-type tomato fruit dramatically increas...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lincoln JE,Fischer RL

    更新日期:1988-10-01 00:00:00

  • Regulation of the Photosynthesis Rhythm in Euglena gracilis: I. Carbonic Anhydrase and Glyceraldehyde-3-Phosphate Dehydrogenase Do Not Regulate the Photosynthesis Rhythm.

    abstract::A circadian rhythm of O(2) evolution has been found in Euglena gracilis, Klebs strain Z. The rhythm persists for at least 5 days in constant dim light and temperature, but damps out in constant bright light. The phase of this rhythm can be shifted by a pulse of bright light and the period length is not changed over a ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lonergan TA,Sargent ML

    更新日期:1978-02-01 00:00:00

  • Dormancy, Turion Formation, and Germination by Different Clones of Spirodela polyrrhiza.

    abstract::Some clones of Spirodela polyrrhiza form dormant bodies called turions which require several weeks of chilling treatment before they proceed to renew growth and develop into vegetative fronds. The individual fronds of Spirodela are less than 5 mm long and can be grown aseptically in liquid culture. Turion formation an...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Perry TO

    更新日期:1968-11-01 00:00:00

  • An auxin-inducible element in soybean SAUR promoters.

    abstract::The soybean SAUR (Small Auxin-Up RNA) genes are transcriptionally induced by exogenous auxins within a few minutes after hormone application. This response is specifically induced by auxins primarily in epidermal and cortical cells within elongation zones of hypocotyls and epicotyls. We have previously shown that an 8...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Li Y,Liu ZB,Shi X,Hagen G,Guilfoyle TJ

    更新日期:1994-09-01 00:00:00


    abstract::An experimental chemical N-[2-(2-oxo-1-imidazolidinyl)ethyl]-N'-phenylurea (EDU), is an effective protectant against acute and chronic foliar injury due to ozone (0(3)) when sprayed on intact leaves or supplied to the plants through soil application. An 0(3)-sensitive snap bean cultivar (Phaseolus vulgaris L. ;Bush Bl...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lee EH,Bennett JH

    更新日期:1982-06-01 00:00:00

  • A simple theory regarding ambimobility of xenobiotics with special reference to the nematicide, oxamyl.

    abstract::A theory is presented to explain the phloem mobility of certain systemic xenobiotics that are not weak acids. It is shown that there is a theoretically optimum permeability that permits optimum circulation through the symplasm and apoplast (including the phloem and xylem) of Solanum tuberosum plants. The optimum perme...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Tyree MT

    更新日期:1979-02-01 00:00:00

  • Electrostatic Changes in Lycopersicon esculentum Root Plasma Membrane Resulting from Salt Stress.

    abstract::Salinity-induced alterations in tomato (Lypersicon esculentum Mill. cv Heinz 1350) root plasma membrane properties were studied and characterized using a membrane vesicle system. Equivalent rates of MgATP-dependent H(+)-transport activity were measured by quinacrine fluorescence (DeltapH) in plasma membrane vesicles i...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Suhayda CG,Giannini JL,Briskin DP,Shannon MC

    更新日期:1990-06-01 00:00:00

  • Effects of decenylsuccinic Acid on p uptake and translocation by barley and winter wheat.

    abstract::After 3 days of exposure to 10(-3) and 10(-4) M decenylsuccinic acid, winter wheat plants wilted and died. Decenylsuccinate at 10(-3) M inhibited (32)P uptake by barley roots and wheat roots and resulted in significant (P

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Green DG,Ferguson WS,Warder FG

    更新日期:1970-01-01 00:00:00

  • Abscisic acid deficiency causes changes in cuticle permeability and pectin composition that influence tomato resistance to Botrytis cinerea.

    abstract::A mutant of tomato (Solanum lycopersicum) with reduced abscisic acid (ABA) production (sitiens) exhibits increased resistance to the necrotrophic fungus Botrytis cinerea. This resistance is correlated with a rapid and strong hydrogen peroxide-driven cell wall fortification response in epidermis cells that is absent in...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Curvers K,Seifi H,Mouille G,de Rycke R,Asselbergh B,Van Hecke A,Vanderschaeghe D,Höfte H,Callewaert N,Van Breusegem F,Höfte M

    更新日期:2010-10-01 00:00:00

  • Photosynthetic Decline from High Temperature Stress during Maturation of Wheat : II. Interaction with Source and Sink Processes.

    abstract::High temperature stress reduces grain growth in wheat (Triticum aestivum L.) by altering source activity and sink capacity. The impact of stress on source and sink interactions in two wheat cultivars of differing source thermotolerance was monitored by analysis of chlorophyll fluorescence transients, Fv (variable fluo...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Harding SA,Guikema JA,Paulsen GM

    更新日期:1990-03-01 00:00:00

  • The enhancement of phototropin-induced phototropic curvature in Arabidopsis occurs via a photoreversible phytochrome A-dependent modulation of auxin responsiveness.

    abstract::The induction of phototropism in etiolated (dark-grown) seedlings exposed to an unidirectional pulse or extended irradiation with low fluence rate blue light (BL) requires the action of the phototropin (nph1) BL receptor. Although cryptochromes and phytochromes are not required for phototropic induction, these photore...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Stowe-Evans EL,Luesse DR,Liscum E

    更新日期:2001-06-01 00:00:00

  • Increased ratio of electron transport to net assimilation rate supports elevated isoprenoid emission rate in eucalypts under drought.

    abstract::Plants undergoing heat and low-CO2 stresses emit large amounts of volatile isoprenoids compared with those in stress-free conditions. One hypothesis posits that the balance between reducing power availability and its use in carbon assimilation determines constitutive isoprenoid emission rates in plants and potentially...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Dani KG,Jamie IM,Prentice IC,Atwell BJ

    更新日期:2014-10-01 00:00:00

  • Differential induction of plant volatile biosynthesis in the lima bean by early and late intermediates of the octadecanoid-signaling pathway.

    abstract::Plants are able to respond to herbivore damage with de novo biosynthesis of an herbivore-characteristic blend of volatiles. The signal transduction initiating volatile biosynthesis may involve the activation of the octadecanoid pathway, as exemplified by the transient increase of endogenous jasmonic acid (JA) in leave...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Koch T,Krumm T,Jung V,Engelberth J,Boland W

    更新日期:1999-09-01 00:00:00

  • Activation of Chloroplast NADP-linked Glyceraldehyde-3-Phosphate Dehydrogenase by the Ferredoxin/Thioredoxin System.

    abstract::NADP-glyceraldehyde-3-P dehydrogenase of spinach (Spinacia oleracea) chloroplasts was activated by thioredoxin that was reduced either photochemically with ferredoxin and ferredoxin-thioredoxin reductase or chemically with dithiothreitol. The activation process that was observed with the soluble protein fraction from ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wolosiuk RA,Buchanan BB

    更新日期:1978-04-01 00:00:00