Purification of the Major Soybean Leaf Acid Phosphatase That Is Increased by Seed-Pod Removal.

Abstract:

:Fruit removal for 5 weeks after flowering increased acid phosphatase activity 10-fold in soybean (Glycine max L. Merr. Var Hobbit) leaves compared with normal seed-pod-bearing plants. The major acid phosphatase activity in leaves was purified over 2700-fold, yielding a single polypeptide of 51 kD with a specific activity of 1353 units/mg protein using p-nitrophenylphosphate as the substrate. Isoelectric focusing demonstrated that the purified protein co-migrated with a majority of the activity that increased in leaves following seed-pod removal. Immunoblot analysis demonstrated that at least part of the increased activity was due to an increased abundance of the phosphatase protein. In situ enzyme activity staining localized most of the total phosphatase activity to vascular tissues, the leaf paraveinal mesophyll cell layer, and the lower epidermis. This distribution and the response to seed-pod removal paralleled previous results for soybean vegetative storage protein (VSP) [alpha] and [beta]. However, in a native polyacrylamide gel the VSP detected by immunological staining of electrophoretically transferred protein did not migrate with the majority of the phosphatase activity. Fractionation of crude leaf protein on concanavalin A-Sepharose yielded a fraction containing 97% of the total VSP but only 0.1% of the total acid phosphatase activity.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Staswick PE,Papa C,Huang JF,Rhee Y

doi

10.1104/pp.104.1.49

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

49-57

issue

1

eissn

0032-0889

issn

1532-2548

pii

104/1/49

journal_volume

104

pub_type

杂志文章
  • A water-soluble chlorophyll protein in cauliflower may be identical to BnD22, a drought-induced, 22-kilodalton protein in rapeseed.

    abstract::A water-soluble chlorophyll protein (WSCP) in cauliflower (Brassica oleracea L.) was purified and its N-terminal sequence was determined. Forty-six of 48 residues of the sequence completely matched those of the drought-induced 22-kD protein (BnD22) in rapeseed (Brassica napus L.). All 40 sequenced residues of WSCP fro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.2.841

    authors: Nishio N,Satoh H

    更新日期:1997-10-01 00:00:00

  • A common fluence threshold for first positive and second positive phototropism in Arabidopsis thaliana.

    abstract::The relationship between the amount of light and the amount of response for any photobiological process can be based on the number of incident quanta per unit time (fluence rate-response) or on the number of incident quanta during a given period of irradiation (fluence-response). Fluence-response and fluence rate-r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.4.1605

    authors: Janoudi A,Poff KL

    更新日期:1990-01-01 00:00:00

  • Sodium absorption by barley roots: its mediation by mechanism 2 of alkali cation transport.

    abstract::When barley roots absorb Na(+) at concentrations ranging from 1 to 50 mm, in the presence of low concentrations of Ca(2+) and K(+), absorption of Na(+) is mediated by carrier mechanism 2 of alkali cation transport, mechanism 1 being unavailable for Na(+) transport under these conditions. The absorption isotherm depict...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.3.319

    authors: Rains DW,Epstein E

    更新日期:1967-03-01 00:00:00

  • Effects of ethylene, kinetin, and calcium on growth and wall composition of pea epicotyls.

    abstract::Ethylene supplied with indoleacetic acid at 0.1 and 1 mum inhibited elongation and enhanced swelling in epicotyls of decapitated and derooted pea seedlings (Pisum sativum L., var. Alaska). These growth responses were correlated with the development of cell walls rich in weak acid-extractable materials and pectic uroni...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.3.450

    authors: Mondal MH,Nance JF

    更新日期:1975-03-01 00:00:00

  • The Arabidopsis family GT43 glycosyltransferases form two functionally nonredundant groups essential for the elongation of glucuronoxylan backbone.

    abstract::There exist four members of family GT43 glycosyltransferases in the Arabidopsis (Arabidopsis thaliana) genome, and mutations of two of them, IRX9 and IRX14, have previously been shown to cause a defect in glucuronoxylan (GX) biosynthesis. However, it is currently unknown whether IRX9 and IRX14 perform the same biochem...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.155309

    authors: Lee C,Teng Q,Huang W,Zhong R,Ye ZH

    更新日期:2010-06-01 00:00:00

  • iTILLING: a personalized approach to the identification of induced mutations in Arabidopsis.

    abstract::TILLING (for Targeting Induced Local Lesions IN Genomes) is a well-established method for identifying plants carrying point mutations in genes of interest. A traditional TILLING project requires a significant investment of time and resources to establish the mutant population and screening infrastructure. Here, we des...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.159897

    authors: Bush SM,Krysan PJ

    更新日期:2010-09-01 00:00:00

  • Insertional inactivation of the methionine s-methyltransferase gene eliminates the s-methylmethionine cycle and increases the methylation ratio.

    abstract::Methionine (Met) S-methyltransferase (MMT) catalyzes the synthesis of S-methyl-Met (SMM) from Met and S-adenosyl-Met (Ado-Met). SMM can be reconverted to Met by donating a methyl group to homocysteine (homo-Cys), and concurrent operation of this reaction and that mediated by MMT sets up the SMM cycle. SMM has been hyp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.018846

    authors: Kocsis MG,Ranocha P,Gage DA,Simon ES,Rhodes D,Peel GJ,Mellema S,Saito K,Awazuhara M,Li C,Meeley RB,Tarczynski MC,Wagner C,Hanson AD

    更新日期:2003-04-01 00:00:00

  • Developmental Physiology of Bean Leaf Plastids II. Negative Contrast Electron Microscopy of Tubular Membranes in Prolamellar Bodies.

    abstract::Proplastids and prolamellar bodies with tubular membranes were isolated from the dark grown primary leaves of bean seedlings (Phaseolus vulgaris L.). The combination of fluorescence microscopy and negative contrast electron microscopy provided the tentative identification of protochlorophyll holochrome as a constituen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.11.1769

    authors: Kahn A

    更新日期:1968-11-01 00:00:00

  • Starch Biosynthesis in Developing Wheat Grain : Evidence against the Direct Involvement of Triose Phosphates in the Metabolic Pathway.

    abstract::We have used (13)C-labeled sugars and nuclear magnetic resonance (NMR) spectrometry to study the metabolic pathway of starch biosynthesis in developing wheat grain (Triticum aestivum cv Mardler). Our aim was to examine the extent of redistribution of (13)C between carbons atoms 1 and 6 of [1-(13)C] or [6-(13)C]glucose...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.2.311

    authors: Keeling PL,Wood JR,Tyson RH,Bridges IG

    更新日期:1988-06-01 00:00:00

  • Steps linking the photosynthetic light reactions to the biological clock require calcium.

    abstract::The effect of modifying calcium concentration on the expression of the photosynthesis circadian rhythm was examined in Euglena gracilis, Klebs strain Z. Expression of the oxygen evolution rhythm required the presence of both extracellular and intracellular calcium. Several treatments were found to uncouple the rate of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.1.110

    authors: Lonergan TA

    更新日期:1990-05-01 00:00:00

  • Functional genomics of eukaryotic photosynthesis using insertional mutagenesis of Chlamydomonas reinhardtii.

    abstract::The unicellular green alga Chlamydomonas reinhardtii is a widely used model organism for studies of oxygenic photosynthesis in eukaryotes. Here we describe the development of a resource for functional genomics of photosynthesis using insertional mutagenesis of the Chlamydomonas nuclear genome. Chlamydomonas cells were...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.055244

    authors: Dent RM,Haglund CM,Chin BL,Kobayashi MC,Niyogi KK

    更新日期:2005-02-01 00:00:00

  • Isolation and Characterization of O-methyltransferases Involved in the Biosynthesis of Glaucine in Glaucium flavum.

    abstract::Transcriptome resources for the medicinal plant Glaucium flavum were searched for orthologs showing identity with characterized O-methyltransferases (OMTs) involved in benzylisoquinoline alkaloid biosynthesis. Seven recombinant proteins were functionally tested using the signature alkaloid substrates for six OMTs: nor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01240

    authors: Chang L,Hagel JM,Facchini PJ

    更新日期:2015-10-01 00:00:00

  • New photoresponses of phycomyces.

    abstract::The influence of light on asexual fruiting and mycelial growth of Phycomyces blakesleeanus Burgeff was studied by means of fruiting body counts and size measurements in cultures on solid media under varied incubation conditions. Five types of photoresponses were shown by ATCC Strain 8743a: (a) photoinduction of giant ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.3.570

    authors: Thornton RM

    更新日期:1973-03-01 00:00:00

  • A novel phospholipase D of Arabidopsis that is activated by oleic acid and associated with the plasma membrane.

    abstract::Oleate-dependent phospholipase D (PLD; EC 3.1.4.4) has been reported in animal systems, but its molecular nature is unkown. Multiple PLDs have been characterized in plants, but none of the previously cloned PLDs exhibits the oleate-activated activity. Here, we describe the biochemical and molecular identification and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Wang C,Wang X

    更新日期:2001-11-01 00:00:00

  • Short-Term Regulation of Crassulacean Acid Metabolism Activity in a Tropical Hemiepiphyte, Clusia uvitana.

    abstract::Diel courses of net CO2 exchange of leaves were studied in Clusia uvitana (Clusiaceae), a tropical Crassulacean acid metabolism (CAM) hemiepiphyte, growing in the crown of a 47-m tall kapok tree on Barro Colorado Island, Panama. Measurements on days without precipitation showed that net uptake of atmospheric CO2 occur...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.3.835

    authors: Zotz G,Winter K

    更新日期:1993-07-01 00:00:00

  • A proposed mechanism for the inhibitory effects of oxidative stress on Rubisco assembly and its subunit expression.

    abstract::In Chlamydomonas reinhardtii, a light-induced oxidative stress shifts the glutathione pool toward its oxidized form, resulting in a translational arrest of the large subunit (LSU) of Rubisco. We show here that the translational arrest of LSU is tightly coordinated with cessation of Rubisco assembly, and both processes...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056341

    authors: Cohen I,Knopf JA,Irihimovitch V,Shapira M

    更新日期:2005-02-01 00:00:00

  • Effects of decenylsuccinic Acid on p uptake and translocation by barley and winter wheat.

    abstract::After 3 days of exposure to 10(-3) and 10(-4) M decenylsuccinic acid, winter wheat plants wilted and died. Decenylsuccinate at 10(-3) M inhibited (32)P uptake by barley roots and wheat roots and resulted in significant (P

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.1.1

    authors: Green DG,Ferguson WS,Warder FG

    更新日期:1970-01-01 00:00:00

  • Short and long term effects of root and shoot chilling of ransom soybean.

    abstract::The immediate short term effects on some physiological processes and the long term effects on morphology and reproductive development of root- and shoot-chilled soybeans (Glycine max L. cv Ransom) were studied. Roots or shoots of 16- or 17-day-old plants were chilled at 10 degrees C for one week, and then rewarmed to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.778

    authors: Musser RL,Thomas SA,Kramer PJ

    更新日期:1983-11-01 00:00:00

  • Endogenous Bioactive Jasmonate Is Composed of a Set of (+)-7-iso-JA-Amino Acid Conjugates.

    abstract::Jasmonates (JAs) regulate a wide range of plant defense and development processes. The bioactive JA is perceived by its receptor COI1 to trigger the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins and subsequently derepress the JAZ-repressed transcription factors for activation of expression of JA-responsive genes....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00906

    authors: Yan J,Li S,Gu M,Yao R,Li Y,Chen J,Yang M,Tong J,Xiao L,Nan F,Xie D

    更新日期:2016-12-01 00:00:00

  • Overexpression of a Calcium-Dependent Protein Kinase Confers Salt and Drought Tolerance in Rice by Preventing Membrane Lipid Peroxidation.

    abstract::The OsCPK4 gene is a member of the complex gene family of calcium-dependent protein kinases in rice (Oryza sativa). Here, we report that OsCPK4 expression is induced by high salinity, drought, and the phytohormone abscisic acid. Moreover, a plasma membrane localization of OsCPK4 was observed by transient expression as...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.230268

    authors: Campo S,Baldrich P,Messeguer J,Lalanne E,Coca M,San Segundo B

    更新日期:2014-06-01 00:00:00

  • Refined annotation of the Arabidopsis genome by complete expressed sequence tag mapping.

    abstract::Expressed sequence tags (ESTs) currently encompass more entries in the public databases than any other form of sequence data. Thus, EST data sets provide a vast resource for gene identification and expression profiling. We have mapped the complete set of 176,915 publicly available Arabidopsis EST sequences onto the Ar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.018101

    authors: Zhu W,Schlueter SD,Brendel V

    更新日期:2003-06-01 00:00:00

  • Further Characterization on the Transport Property of Plasmalemma NADH Oxidation System in Isolated Corn Root Protoplasts.

    abstract::Recent experiments show that exogenous NADH increases the O(2) consumption and uptake of inorganic ions into isolated corn (Zea mays L. Pioneer Hybrid 3320) root protoplasts (Lin 1982, Proc Natl Acad Sci USA 79: 3773-3776). A mild treatment of protoplasts with trypsin released most of the NADH oxidation system from th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.2.219

    authors: Lin W

    更新日期:1984-02-01 00:00:00

  • Biosynthesis of the Dihydrochalcone Sweetener Trilobatin Requires Phloretin Glycosyltransferase2.

    abstract::Epidemics of obesity and type 2 diabetes drive strong consumer interest in plant-based low-calorie sweeteners. Trilobatin is a sweetener found at high concentrations in the leaves of a range of crabapple (Malus) species, but not in domesticated apple (Malus × domestica) leaves, which contain trilobatin's bitter positi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00807

    authors: Wang Y,Yauk YK,Zhao Q,Hamiaux C,Xiao Z,Gunaseelan K,Zhang L,Tomes S,López-Girona E,Cooney J,Li H,Chagné D,Ma F,Li P,Atkinson RG

    更新日期:2020-10-01 00:00:00

  • Appearance of an Alternate Pathway Cyanide-resistant during Germination of Seeds of Cicer arietinum.

    abstract::The combined action of the inhibitors antimycin A and cyanide with benzohydroxamic acid indicates the presence of a cyanide-resistant pathway of respiration in chick pea (Cicer arietinum L.) seeds. The appearance of this pathway takes place during germination. During the first 12 hours of germination, the respiration ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.4.524

    authors: Burguillo Pde L,Nicolás G

    更新日期:1977-10-01 00:00:00

  • Saturation Kinetics of the Velocity of Stomatal Closing in Response to CO(2).

    abstract::Stomatal closing movements in response to changes from CO(2)-free to CO(2)-containing air were recorded in leaf sections of Zea mays using air flow porometers. The response to CO(2) was fast; the shortest lag between the application of 300 microliters CO(2) per liter of air and the beginning of a stomatal response was...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.2.229

    authors: Raschke K

    更新日期:1972-02-01 00:00:00

  • Lignification in sugarcane: biochemical characterization, gene discovery, and expression analysis in two genotypes contrasting for lignin content.

    abstract::Sugarcane (Saccharum spp.) is currently one of the most efficient crops in the production of first-generation biofuels. However, the bagasse represents an additional abundant lignocellulosic resource that has the potential to increase the ethanol production per plant. To achieve a more efficient conversion of bagasse ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.225250

    authors: Bottcher A,Cesarino I,Santos AB,Vicentini R,Mayer JL,Vanholme R,Morreel K,Goeminne G,Moura JC,Nobile PM,Carmello-Guerreiro SM,Anjos IA,Creste S,Boerjan W,Landell MG,Mazzafera P

    更新日期:2013-12-01 00:00:00

  • Nod factor elicits two separable calcium responses in Medicago truncatula root hair cells.

    abstract::Modulation of intracellular calcium levels plays a key role in the transduction of many biological signals. Here, we characterize early calcium responses of wild-type and mutant Medicago truncatula plants to nodulation factors produced by the bacterial symbiont Sinorhizobium meliloti using a dual-dye ratiometric imagi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.005546

    authors: Shaw SL,Long SR

    更新日期:2003-03-01 00:00:00

  • The DEK1 Calpain Linker Functions in Three-Dimensional Body Patterning in Physcomitrella patens.

    abstract::The DEFECTIVE KERNEL1 (DEK1) calpain is a conserved 240-kD key regulator of three-dimensional body patterning in land plants acting via mitotic cell plane positioning. The activity of the cytosolic C-terminal calpain protease is regulated by the membrane-anchored DEK1 MEM, which is connected to the calpain via the 600...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00925

    authors: Johansen W,Ako AE,Demko V,Perroud PF,Rensing SA,Mekhlif AK,Olsen OA

    更新日期:2016-10-01 00:00:00

  • Regions of differential cell elongation and mitosis, and root meristem morphology in different tissues of geotropically stimulated maize root apices.

    abstract::We examined cell length, mitosis, and root meristem "cuticle" in different tissues of geostimulated, red light-exposed primary roots of corn (Zea Mays, Wisconsin hybrid 64A x 22R). The examination was done at 15-minute intervals for a period of 240 minutes. Differences in cell elongation between the upper and lower si...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.1.7

    authors: Shen-Miller J,McNitt RE,Wojciechowski M

    更新日期:1978-01-01 00:00:00

  • Dissection of the control of anion homeostasis by associative transcriptomics in Brassica napus.

    abstract::To assess the variation in nutrient homeostasis in oilseed rape and to identify the genes responsible for this variation, we determined foliar anion levels in a diversity panel of Brassica napus accessions, 84 of which had been genotyped previously using messenger RNA sequencing. We applied associative transcriptomics...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.239947

    authors: Koprivova A,Harper AL,Trick M,Bancroft I,Kopriva S

    更新日期:2014-09-01 00:00:00